q5 high gc enhancer buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs q5 high gc enhancer buffer
    Q5 High Gc Enhancer Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 high gc enhancer buffer/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    q5 high gc enhancer buffer - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Molecular, phylogenetic and developmental analyses of Sall proteins in bilaterians
    Article Snippet: Paragraph title: Cloning, sequencing and RNA probe generation ... PCR amplification reactions were performed with Phusion HF DNA polymerase, Q5 high-fidelity DNA polymerase and Q5 high GC enhancer buffer (New England Biolabs, Ipswich, MA), according to the manufacturer’s suggested ratios.

    Amplification:

    Article Title: Molecular, phylogenetic and developmental analyses of Sall proteins in bilaterians
    Article Snippet: .. PCR amplification reactions were performed with Phusion HF DNA polymerase, Q5 high-fidelity DNA polymerase and Q5 high GC enhancer buffer (New England Biolabs, Ipswich, MA), according to the manufacturer’s suggested ratios. .. Amplified PCR products were run on 1% agarose gels, gel-purified (GeneClean Turbo kit, MP Biomedicals, Solon, OH) and cloned into pGem-T Easy vector (Promega, Madison, WI).

    Synthesized:

    Article Title: Molecular, phylogenetic and developmental analyses of Sall proteins in bilaterians
    Article Snippet: PCR amplification reactions were performed with Phusion HF DNA polymerase, Q5 high-fidelity DNA polymerase and Q5 high GC enhancer buffer (New England Biolabs, Ipswich, MA), according to the manufacturer’s suggested ratios. .. Digoxigenin (DIG)-labeled and fluorescein (FITC)-labeled antisense and sense RNAs were synthesized from purified pGEM-T easy plasmid DNA that was amplified using SP6 and T7 primers via PCR.

    Spectrophotometry:

    Article Title: Molecular, phylogenetic and developmental analyses of Sall proteins in bilaterians
    Article Snippet: The purity and concentration of total RNA was verified with a NanoDrop ND-1000 Spectrophotometer (Thermo Scientific, Wilmington, DE) and approximately 1 µg of total RNA from each developmental stage was used to synthesize cDNA (iScript cDNA Synthesis kit, Bio-Rad, Hercules, CA). .. PCR amplification reactions were performed with Phusion HF DNA polymerase, Q5 high-fidelity DNA polymerase and Q5 high GC enhancer buffer (New England Biolabs, Ipswich, MA), according to the manufacturer’s suggested ratios.

    Purification:

    Article Title: Molecular, phylogenetic and developmental analyses of Sall proteins in bilaterians
    Article Snippet: High-quality total RNA from embryos of the gastropods L. gigantea and C. fornicata was extracted using TriZol and purification methods followed the manufacturer’s suggested protocol. .. PCR amplification reactions were performed with Phusion HF DNA polymerase, Q5 high-fidelity DNA polymerase and Q5 high GC enhancer buffer (New England Biolabs, Ipswich, MA), according to the manufacturer’s suggested ratios.

    Concentration Assay:

    Article Title: Molecular, phylogenetic and developmental analyses of Sall proteins in bilaterians
    Article Snippet: The purity and concentration of total RNA was verified with a NanoDrop ND-1000 Spectrophotometer (Thermo Scientific, Wilmington, DE) and approximately 1 µg of total RNA from each developmental stage was used to synthesize cDNA (iScript cDNA Synthesis kit, Bio-Rad, Hercules, CA). .. PCR amplification reactions were performed with Phusion HF DNA polymerase, Q5 high-fidelity DNA polymerase and Q5 high GC enhancer buffer (New England Biolabs, Ipswich, MA), according to the manufacturer’s suggested ratios.

    Polymerase Chain Reaction:

    Article Title: Molecular, phylogenetic and developmental analyses of Sall proteins in bilaterians
    Article Snippet: .. PCR amplification reactions were performed with Phusion HF DNA polymerase, Q5 high-fidelity DNA polymerase and Q5 high GC enhancer buffer (New England Biolabs, Ipswich, MA), according to the manufacturer’s suggested ratios. .. Amplified PCR products were run on 1% agarose gels, gel-purified (GeneClean Turbo kit, MP Biomedicals, Solon, OH) and cloned into pGem-T Easy vector (Promega, Madison, WI).

    Labeling:

    Article Title: Molecular, phylogenetic and developmental analyses of Sall proteins in bilaterians
    Article Snippet: PCR amplification reactions were performed with Phusion HF DNA polymerase, Q5 high-fidelity DNA polymerase and Q5 high GC enhancer buffer (New England Biolabs, Ipswich, MA), according to the manufacturer’s suggested ratios. .. Template DNA was used with SP6 and T7 RNA polymerase (Invitrogen, Carlsbad, CA, USA), DIG and fluorescein labeling mix (Roche, Indianapolis, IN, USA) to generate probes.

    Sequencing:

    Article Title: Molecular, phylogenetic and developmental analyses of Sall proteins in bilaterians
    Article Snippet: Paragraph title: Cloning, sequencing and RNA probe generation ... PCR amplification reactions were performed with Phusion HF DNA polymerase, Q5 high-fidelity DNA polymerase and Q5 high GC enhancer buffer (New England Biolabs, Ipswich, MA), according to the manufacturer’s suggested ratios.

    Plasmid Preparation:

    Article Title: Molecular, phylogenetic and developmental analyses of Sall proteins in bilaterians
    Article Snippet: PCR amplification reactions were performed with Phusion HF DNA polymerase, Q5 high-fidelity DNA polymerase and Q5 high GC enhancer buffer (New England Biolabs, Ipswich, MA), according to the manufacturer’s suggested ratios. .. Amplified PCR products were run on 1% agarose gels, gel-purified (GeneClean Turbo kit, MP Biomedicals, Solon, OH) and cloned into pGem-T Easy vector (Promega, Madison, WI).

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  • 95
    New England Biolabs q5 high gc enhancer buffer
    Q5 High Gc Enhancer Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 high gc enhancer buffer/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    q5 high gc enhancer buffer - by Bioz Stars, 2020-04
    95/100 stars
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