q5 hifi polymerase  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    New England Biolabs q5 hifi polymerase
    Q5 Hifi Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 hifi polymerase/product/New England Biolabs
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    q5 hifi polymerase - by Bioz Stars, 2020-04
    94/100 stars

    Images

    Related Articles

    Electroporation:

    Article Title: Genome-wide CRISPR screen for essential cell growth mediators in mutant KRAS colorectal cancers
    Article Snippet: The human GeCKO v2 A library pooled plasmid (lentiCRISPR v2) was obtained from Addgene (cat # 1000000048) and amplified according to the recommended protocols by electroporation in Lucigen Endura electrocompetent cells. .. Full-length oligonucleotides were amplified by PCR using Q5 HiFi polymerase (New England Biolabs) and size-selected on 2% agarose gels.

    Transfection:

    Article Title: Endosomal Escape of Antisense Oligonucleotides Internalized by Stabilin Receptors Is Regulated by Rab5C and EEA1 During Endosomal Maturation
    Article Snippet: Rab5C plasmid was PCR amplified using Q5-HiFi Polymerase (NEB) for 18 cycles. .. Rab5CS35 N plasmids were transfected into 190-HARE cells and incubated for 24 h, followed by siRNA treatment.

    Amplification:

    Article Title: Endosomal Escape of Antisense Oligonucleotides Internalized by Stabilin Receptors Is Regulated by Rab5C and EEA1 During Endosomal Maturation
    Article Snippet: .. Rab5C plasmid was PCR amplified using Q5-HiFi Polymerase (NEB) for 18 cycles. .. Samples were Dpn1 digested for 1 h to remove methylated template DNA, and heat shock transformed into competent Escherichia coli cells.

    Article Title: Repurposing CRISPR/Cas9 for in situ functional assays
    Article Snippet: Off-target loci (with the exception of #4 and #7) ( ) were amplified by PCR using Phusion HiFi polymerase (New England Biolabs) with five cycles of annealing temperature at 56°C followed by 20 cycles with an annealing temperature of 68°C. .. Conditions used to PCR off-target locus #4 were the same; however, Q5 HiFi polymerase (New England Biolabs) was used.

    Article Title: Genome-wide CRISPR screen for essential cell growth mediators in mutant KRAS colorectal cancers
    Article Snippet: .. Full-length oligonucleotides were amplified by PCR using Q5 HiFi polymerase (New England Biolabs) and size-selected on 2% agarose gels. .. PCR inserts were cloned into BsmBI-digested lentiGuide-Puro (Addgene # 52963) using Gibson assembly (New England Biolabs), transformed into Endura electrocompetent cells, and amplified similarly to the GeCKO library.

    Article Title: VCP/p97 Is a Proviral Host Factor for Replication of Chikungunya Virus and Other Alphaviruses
    Article Snippet: .. Plasmid Contruction Valosin-containing protein and VCP domains N and ND1 were amplified by PCR from HEK293T cDNA using the primers indicated in and Q5 HiFi polymerase (NEB) following manufacturer recommendations. .. PCR were ligated in p3XFLAG-CMVTM -10 (Sigma) using Not I and Hin dIII restriction digestions (NEB) to generate flag-tagged VCP full length expression plasmid (FL) and mutants N and ND1.

    Clone Assay:

    Article Title: Genome-wide CRISPR screen for essential cell growth mediators in mutant KRAS colorectal cancers
    Article Snippet: Full-length oligonucleotides were amplified by PCR using Q5 HiFi polymerase (New England Biolabs) and size-selected on 2% agarose gels. .. PCR inserts were cloned into BsmBI-digested lentiGuide-Puro (Addgene # 52963) using Gibson assembly (New England Biolabs), transformed into Endura electrocompetent cells, and amplified similarly to the GeCKO library.

    Methylation:

    Article Title: Endosomal Escape of Antisense Oligonucleotides Internalized by Stabilin Receptors Is Regulated by Rab5C and EEA1 During Endosomal Maturation
    Article Snippet: Rab5C plasmid was PCR amplified using Q5-HiFi Polymerase (NEB) for 18 cycles. .. Samples were Dpn1 digested for 1 h to remove methylated template DNA, and heat shock transformed into competent Escherichia coli cells.

    Mutagenesis:

    Article Title: Endosomal Escape of Antisense Oligonucleotides Internalized by Stabilin Receptors Is Regulated by Rab5C and EEA1 During Endosomal Maturation
    Article Snippet: Paragraph title: Rab5C (S35 N) point mutant ... Rab5C plasmid was PCR amplified using Q5-HiFi Polymerase (NEB) for 18 cycles.

    Article Title: Repurposing CRISPR/Cas9 for in situ functional assays
    Article Snippet: Paragraph title: Deep sequencing to assess mutation spectrum and targeting specificity ... Conditions used to PCR off-target locus #4 were the same; however, Q5 HiFi polymerase (New England Biolabs) was used.

    Article Title: VCP/p97 Is a Proviral Host Factor for Replication of Chikungunya Virus and Other Alphaviruses
    Article Snippet: Plasmid Contruction Valosin-containing protein and VCP domains N and ND1 were amplified by PCR from HEK293T cDNA using the primers indicated in and Q5 HiFi polymerase (NEB) following manufacturer recommendations. .. D2 domain was generated using site-directed mutagenesis (SDM) kit from NEB following manufacturer recommendations and primers given in .

    CRISPR:

    Article Title: Repurposing CRISPR/Cas9 for in situ functional assays
    Article Snippet: Genomic regions flanking p53 and predicted CRISPR target sites were amplified using barcoded primers with engineered adaptor regions. .. Conditions used to PCR off-target locus #4 were the same; however, Q5 HiFi polymerase (New England Biolabs) was used.

    Incubation:

    Article Title: Endosomal Escape of Antisense Oligonucleotides Internalized by Stabilin Receptors Is Regulated by Rab5C and EEA1 During Endosomal Maturation
    Article Snippet: Rab5C plasmid was PCR amplified using Q5-HiFi Polymerase (NEB) for 18 cycles. .. Rab5CS35 N plasmids were transfected into 190-HARE cells and incubated for 24 h, followed by siRNA treatment.

    Real-time Polymerase Chain Reaction:

    Article Title: Endosomal Escape of Antisense Oligonucleotides Internalized by Stabilin Receptors Is Regulated by Rab5C and EEA1 During Endosomal Maturation
    Article Snippet: Rab5C plasmid was PCR amplified using Q5-HiFi Polymerase (NEB) for 18 cycles. .. After 24 h of siRNA treatment, PS-ASOs (0.1 μM) were added and incubated for 24 h, followed by qPCR targeting Malat-1 as described above.

    Polymerase Chain Reaction:

    Article Title: Endosomal Escape of Antisense Oligonucleotides Internalized by Stabilin Receptors Is Regulated by Rab5C and EEA1 During Endosomal Maturation
    Article Snippet: .. Rab5C plasmid was PCR amplified using Q5-HiFi Polymerase (NEB) for 18 cycles. .. Samples were Dpn1 digested for 1 h to remove methylated template DNA, and heat shock transformed into competent Escherichia coli cells.

    Article Title: Repurposing CRISPR/Cas9 for in situ functional assays
    Article Snippet: .. Conditions used to PCR off-target locus #4 were the same; however, Q5 HiFi polymerase (New England Biolabs) was used. .. To amplify off-target locus #7, a touchdown PCR protocol with Q5 HiFi polymerase (New England Biolabs) was used.

    Article Title: Genome-wide CRISPR screen for essential cell growth mediators in mutant KRAS colorectal cancers
    Article Snippet: .. Full-length oligonucleotides were amplified by PCR using Q5 HiFi polymerase (New England Biolabs) and size-selected on 2% agarose gels. .. PCR inserts were cloned into BsmBI-digested lentiGuide-Puro (Addgene # 52963) using Gibson assembly (New England Biolabs), transformed into Endura electrocompetent cells, and amplified similarly to the GeCKO library.

    Article Title: VCP/p97 Is a Proviral Host Factor for Replication of Chikungunya Virus and Other Alphaviruses
    Article Snippet: .. Plasmid Contruction Valosin-containing protein and VCP domains N and ND1 were amplified by PCR from HEK293T cDNA using the primers indicated in and Q5 HiFi polymerase (NEB) following manufacturer recommendations. .. PCR were ligated in p3XFLAG-CMVTM -10 (Sigma) using Not I and Hin dIII restriction digestions (NEB) to generate flag-tagged VCP full length expression plasmid (FL) and mutants N and ND1.

    Article Title: Repurposing CRISPR/Cas9 for in situ functional assays
    Article Snippet: Conditions used to PCR off-target locus #4 were the same; however, Q5 HiFi polymerase (New England Biolabs) was used. .. To amplify off-target locus #7, a touchdown PCR protocol with Q5 HiFi polymerase (New England Biolabs) was used.

    Generated:

    Article Title: VCP/p97 Is a Proviral Host Factor for Replication of Chikungunya Virus and Other Alphaviruses
    Article Snippet: Plasmid Contruction Valosin-containing protein and VCP domains N and ND1 were amplified by PCR from HEK293T cDNA using the primers indicated in and Q5 HiFi polymerase (NEB) following manufacturer recommendations. .. D2 domain was generated using site-directed mutagenesis (SDM) kit from NEB following manufacturer recommendations and primers given in .

    Low Protein Binding:

    Article Title: Genome-wide CRISPR screen for essential cell growth mediators in mutant KRAS colorectal cancers
    Article Snippet: Full-length oligonucleotides were amplified by PCR using Q5 HiFi polymerase (New England Biolabs) and size-selected on 2% agarose gels. .. The supernatant was harvested after 60 h, centrifuged at 3000 rpm at 4°C for 10 min, filtered through a 0.45 μm low protein binding membrane (Millipore), and concentrated using an Amicon Ultra-15 Centrifugal Filter (Millipore) for 40 min at 4°C at 4000 rpm.

    Expressing:

    Article Title: VCP/p97 Is a Proviral Host Factor for Replication of Chikungunya Virus and Other Alphaviruses
    Article Snippet: Plasmid Contruction Valosin-containing protein and VCP domains N and ND1 were amplified by PCR from HEK293T cDNA using the primers indicated in and Q5 HiFi polymerase (NEB) following manufacturer recommendations. .. PCR were ligated in p3XFLAG-CMVTM -10 (Sigma) using Not I and Hin dIII restriction digestions (NEB) to generate flag-tagged VCP full length expression plasmid (FL) and mutants N and ND1.

    Touchdown PCR:

    Article Title: Repurposing CRISPR/Cas9 for in situ functional assays
    Article Snippet: Conditions used to PCR off-target locus #4 were the same; however, Q5 HiFi polymerase (New England Biolabs) was used. .. To amplify off-target locus #7, a touchdown PCR protocol with Q5 HiFi polymerase (New England Biolabs) was used.

    Article Title: Repurposing CRISPR/Cas9 for in situ functional assays
    Article Snippet: .. To amplify off-target locus #7, a touchdown PCR protocol with Q5 HiFi polymerase (New England Biolabs) was used. ..

    Sequencing:

    Article Title: Repurposing CRISPR/Cas9 for in situ functional assays
    Article Snippet: Paragraph title: Deep sequencing to assess mutation spectrum and targeting specificity ... Conditions used to PCR off-target locus #4 were the same; however, Q5 HiFi polymerase (New England Biolabs) was used.

    Article Title: Genome-wide CRISPR screen for essential cell growth mediators in mutant KRAS colorectal cancers
    Article Snippet: For the secondary focused validation sgRNA library, oligonucleotide pools (CustomArray) were obtained with variable 20 bp sgRNA sequences flanked by universal PCR primers (74 bp total synthesized sequence, see for full sequences). .. Full-length oligonucleotides were amplified by PCR using Q5 HiFi polymerase (New England Biolabs) and size-selected on 2% agarose gels.

    Article Title: VCP/p97 Is a Proviral Host Factor for Replication of Chikungunya Virus and Other Alphaviruses
    Article Snippet: Plasmid Contruction Valosin-containing protein and VCP domains N and ND1 were amplified by PCR from HEK293T cDNA using the primers indicated in and Q5 HiFi polymerase (NEB) following manufacturer recommendations. .. All plasmids were verified by sanger sequencing before use and correct expression was confirmed by western blot using monoclonal mouse anti-flag M2 (Sigma, ref: F3165) (not shown).

    Western Blot:

    Article Title: VCP/p97 Is a Proviral Host Factor for Replication of Chikungunya Virus and Other Alphaviruses
    Article Snippet: Plasmid Contruction Valosin-containing protein and VCP domains N and ND1 were amplified by PCR from HEK293T cDNA using the primers indicated in and Q5 HiFi polymerase (NEB) following manufacturer recommendations. .. All plasmids were verified by sanger sequencing before use and correct expression was confirmed by western blot using monoclonal mouse anti-flag M2 (Sigma, ref: F3165) (not shown).

    Transformation Assay:

    Article Title: Endosomal Escape of Antisense Oligonucleotides Internalized by Stabilin Receptors Is Regulated by Rab5C and EEA1 During Endosomal Maturation
    Article Snippet: Rab5C plasmid was PCR amplified using Q5-HiFi Polymerase (NEB) for 18 cycles. .. Samples were Dpn1 digested for 1 h to remove methylated template DNA, and heat shock transformed into competent Escherichia coli cells.

    Article Title: Genome-wide CRISPR screen for essential cell growth mediators in mutant KRAS colorectal cancers
    Article Snippet: Full-length oligonucleotides were amplified by PCR using Q5 HiFi polymerase (New England Biolabs) and size-selected on 2% agarose gels. .. PCR inserts were cloned into BsmBI-digested lentiGuide-Puro (Addgene # 52963) using Gibson assembly (New England Biolabs), transformed into Endura electrocompetent cells, and amplified similarly to the GeCKO library.

    Synthesized:

    Article Title: Genome-wide CRISPR screen for essential cell growth mediators in mutant KRAS colorectal cancers
    Article Snippet: For the secondary focused validation sgRNA library, oligonucleotide pools (CustomArray) were obtained with variable 20 bp sgRNA sequences flanked by universal PCR primers (74 bp total synthesized sequence, see for full sequences). .. Full-length oligonucleotides were amplified by PCR using Q5 HiFi polymerase (New England Biolabs) and size-selected on 2% agarose gels.

    Plasmid Preparation:

    Article Title: Endosomal Escape of Antisense Oligonucleotides Internalized by Stabilin Receptors Is Regulated by Rab5C and EEA1 During Endosomal Maturation
    Article Snippet: .. Rab5C plasmid was PCR amplified using Q5-HiFi Polymerase (NEB) for 18 cycles. .. Samples were Dpn1 digested for 1 h to remove methylated template DNA, and heat shock transformed into competent Escherichia coli cells.

    Article Title: Genome-wide CRISPR screen for essential cell growth mediators in mutant KRAS colorectal cancers
    Article Snippet: The human GeCKO v2 A library pooled plasmid (lentiCRISPR v2) was obtained from Addgene (cat # 1000000048) and amplified according to the recommended protocols by electroporation in Lucigen Endura electrocompetent cells. .. Full-length oligonucleotides were amplified by PCR using Q5 HiFi polymerase (New England Biolabs) and size-selected on 2% agarose gels.

    Article Title: VCP/p97 Is a Proviral Host Factor for Replication of Chikungunya Virus and Other Alphaviruses
    Article Snippet: .. Plasmid Contruction Valosin-containing protein and VCP domains N and ND1 were amplified by PCR from HEK293T cDNA using the primers indicated in and Q5 HiFi polymerase (NEB) following manufacturer recommendations. .. PCR were ligated in p3XFLAG-CMVTM -10 (Sigma) using Not I and Hin dIII restriction digestions (NEB) to generate flag-tagged VCP full length expression plasmid (FL) and mutants N and ND1.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    New England Biolabs nebnext q5
    Nebnext Q5, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext q5/product/New England Biolabs
    Average 90 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    nebnext q5 - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    Image Search Results