q5 enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs q5 enzyme
    Q5 Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 enzyme/product/New England Biolabs
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    q5 enzyme - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons
    Article Snippet: Briefly, genes were amplified by PCR using the Q5 enzyme (New England Biolabs) and assembled into plasmids for the generation of AAV vectors using Gibson Assembly (New England Biolabs). .. Correct clones for each vector were amplified in Stbl2 Escherichia coli (Invitrogen), and the DNA was purified in endotoxin-free conditions (Qiagen).

    Article Title: Development of a cyanobacterial heterologous polyketide production platform
    Article Snippet: PCR amplifications were carried out with Q5 enzyme (New England BioLabs) according to the manufacturer’s instructions. .. Cloning reactions were performed by Gibson assembly using GeneArt Seamless Cloning and Assembly Kit (Life Technologies).

    Article Title: Interferon-Dependent Induction of Clr-b during Mouse Cytomegalovirus Infection Protects Bystander Cells from Natural Killer Cells via NKR-P1B-Mediated Inhibition
    Article Snippet: PCR products were cloned into the luciferase vectors pGL3-Basic or pGL4.22 (Promega) and then sequenced (Macrogen Inc., South Korea, or TCAG Sequencing, Hospital for Sick Children, Toronto, ON, Canada). .. To overexpress IRF3/7/9, their respective coding sequences were PCR amplified from MCMV-infected NIH3T3 cDNA using the Q5 enzyme (New England Biolabs) and the primers listed in online supplementary Table .

    Article Title: The conserved RNA recognition motif and C3H1 domain of the Not4 ubiquitin ligase regulate in vivo ligase function
    Article Snippet: .. Cloning The wild-type NOT4 open reading frame was amplified as a C-terminal mono-FLAG fusion using Q5 enzyme (New England Biolabs) and cloned into the Xba1 and Eco RI digested plasmid p416ADH . .. Mutations were introduced using the QuikChange Mutagenesis kit from Agilent Technologies and confirmed by sequencing.

    Article Title: The conserved RNA recognition motif and C3H1 domain of the Not4 ubiquitin ligase regulate in vivo ligase function
    Article Snippet: .. The wild-type NOT4 open reading frame was amplified as a C-terminal mono-FLAG fusion using Q5 enzyme (New England Biolabs) and cloned into the Xba1 and Eco RI digested plasmid p416 ADH . .. Mutations were introduced using the QuikChange Mutagenesis kit from Agilent Technologies and confirmed by sequencing.

    Transfection:

    Article Title: Interferon-Dependent Induction of Clr-b during Mouse Cytomegalovirus Infection Protects Bystander Cells from Natural Killer Cells via NKR-P1B-Mediated Inhibition
    Article Snippet: The pRL-TK vector was used as a control for transfection efficiency (Promega). .. To overexpress IRF3/7/9, their respective coding sequences were PCR amplified from MCMV-infected NIH3T3 cDNA using the Q5 enzyme (New England Biolabs) and the primers listed in online supplementary Table .

    Amplification:

    Article Title: Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons
    Article Snippet: .. Briefly, genes were amplified by PCR using the Q5 enzyme (New England Biolabs) and assembled into plasmids for the generation of AAV vectors using Gibson Assembly (New England Biolabs). .. Correct clones for each vector were amplified in Stbl2 Escherichia coli (Invitrogen), and the DNA was purified in endotoxin-free conditions (Qiagen).

    Article Title: Interferon-Dependent Induction of Clr-b during Mouse Cytomegalovirus Infection Protects Bystander Cells from Natural Killer Cells via NKR-P1B-Mediated Inhibition
    Article Snippet: .. To overexpress IRF3/7/9, their respective coding sequences were PCR amplified from MCMV-infected NIH3T3 cDNA using the Q5 enzyme (New England Biolabs) and the primers listed in online supplementary Table . .. The sequences were ligated into pcDNA3.1 (Life Technologies) and sequenced.

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
    Article Snippet: .. First, we have generated an NGS dataset generated from a single PCR amplification using the Q5 enzyme (NEB), as previously described for the T2 experiment, of three different templates: (AC)20 , (AC)25 and (AC)30 , each using three serially diluted templates (by 10-fold each). ..

    Article Title: The conserved RNA recognition motif and C3H1 domain of the Not4 ubiquitin ligase regulate in vivo ligase function
    Article Snippet: .. Cloning The wild-type NOT4 open reading frame was amplified as a C-terminal mono-FLAG fusion using Q5 enzyme (New England Biolabs) and cloned into the Xba1 and Eco RI digested plasmid p416ADH . .. Mutations were introduced using the QuikChange Mutagenesis kit from Agilent Technologies and confirmed by sequencing.

    Article Title: The conserved RNA recognition motif and C3H1 domain of the Not4 ubiquitin ligase regulate in vivo ligase function
    Article Snippet: .. The wild-type NOT4 open reading frame was amplified as a C-terminal mono-FLAG fusion using Q5 enzyme (New England Biolabs) and cloned into the Xba1 and Eco RI digested plasmid p416 ADH . .. Mutations were introduced using the QuikChange Mutagenesis kit from Agilent Technologies and confirmed by sequencing.

    DNA Purification:

    Article Title: Development of a cyanobacterial heterologous polyketide production platform
    Article Snippet: PCR amplifications were carried out with Q5 enzyme (New England BioLabs) according to the manufacturer’s instructions. .. DNA purification and concentration of restriction digests and PCR products were performed with DNA Clean & Concentrator TM-5 (Zymo).

    Concentration Assay:

    Article Title: Development of a cyanobacterial heterologous polyketide production platform
    Article Snippet: PCR amplifications were carried out with Q5 enzyme (New England BioLabs) according to the manufacturer’s instructions. .. DNA purification and concentration of restriction digests and PCR products were performed with DNA Clean & Concentrator TM-5 (Zymo).

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
    Article Snippet: First, we have generated an NGS dataset generated from a single PCR amplification using the Q5 enzyme (NEB), as previously described for the T2 experiment, of three different templates: (AC)20 , (AC)25 and (AC)30 , each using three serially diluted templates (by 10-fold each). .. First, we performed a small-scale PCR enzyme comparison by applying five commercially available PCR enzymes on the same synthetic templates as used above at an equal template concentration (using a subset of the generated data of Q5 from the above-mentioned experiment (1) and four other enzymes).

    Mutagenesis:

    Article Title: Probing remote residues important for catalysis in Escherichia coli ornithine transcarbamoylase
    Article Snippet: .. Variants were prepared with a Quikchange site-directed mutagenesis kit (Agilent) or by two-stage PCR with Q5 enzyme (New England Biolabs) using mutagenic DNA primers from Eurofins Operon and verified by DNA sequencing (Eton Bioscience, Charlestown, MA). .. Protein expression and purification WT OTC and variants were expressed in BL21 (DE3) Tuner cells (Novagen) in 1 L of Luria broth with 25 μg/mL chloramphenicol at 37°C.

    Article Title: The conserved RNA recognition motif and C3H1 domain of the Not4 ubiquitin ligase regulate in vivo ligase function
    Article Snippet: Cloning The wild-type NOT4 open reading frame was amplified as a C-terminal mono-FLAG fusion using Q5 enzyme (New England Biolabs) and cloned into the Xba1 and Eco RI digested plasmid p416ADH . .. Mutations were introduced using the QuikChange Mutagenesis kit from Agilent Technologies and confirmed by sequencing.

    Article Title: The conserved RNA recognition motif and C3H1 domain of the Not4 ubiquitin ligase regulate in vivo ligase function
    Article Snippet: The wild-type NOT4 open reading frame was amplified as a C-terminal mono-FLAG fusion using Q5 enzyme (New England Biolabs) and cloned into the Xba1 and Eco RI digested plasmid p416 ADH . .. Mutations were introduced using the QuikChange Mutagenesis kit from Agilent Technologies and confirmed by sequencing.

    Serial Dilution:

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
    Article Snippet: Experimental validation of the model by using controlled synthetic templates Controlled amplifications of synthetic STRs in a serial dilution experiment. .. First, we have generated an NGS dataset generated from a single PCR amplification using the Q5 enzyme (NEB), as previously described for the T2 experiment, of three different templates: (AC)20 , (AC)25 and (AC)30 , each using three serially diluted templates (by 10-fold each).

    Next-Generation Sequencing:

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
    Article Snippet: .. First, we have generated an NGS dataset generated from a single PCR amplification using the Q5 enzyme (NEB), as previously described for the T2 experiment, of three different templates: (AC)20 , (AC)25 and (AC)30 , each using three serially diluted templates (by 10-fold each). ..

    Polymerase Chain Reaction:

    Article Title: Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons
    Article Snippet: .. Briefly, genes were amplified by PCR using the Q5 enzyme (New England Biolabs) and assembled into plasmids for the generation of AAV vectors using Gibson Assembly (New England Biolabs). .. Correct clones for each vector were amplified in Stbl2 Escherichia coli (Invitrogen), and the DNA was purified in endotoxin-free conditions (Qiagen).

    Article Title: Development of a cyanobacterial heterologous polyketide production platform
    Article Snippet: .. PCR amplifications were carried out with Q5 enzyme (New England BioLabs) according to the manufacturer’s instructions. .. DNA purification and concentration of restriction digests and PCR products were performed with DNA Clean & Concentrator TM-5 (Zymo).

    Article Title: Interferon-Dependent Induction of Clr-b during Mouse Cytomegalovirus Infection Protects Bystander Cells from Natural Killer Cells via NKR-P1B-Mediated Inhibition
    Article Snippet: .. To overexpress IRF3/7/9, their respective coding sequences were PCR amplified from MCMV-infected NIH3T3 cDNA using the Q5 enzyme (New England Biolabs) and the primers listed in online supplementary Table . .. The sequences were ligated into pcDNA3.1 (Life Technologies) and sequenced.

    Article Title: Probing remote residues important for catalysis in Escherichia coli ornithine transcarbamoylase
    Article Snippet: .. Variants were prepared with a Quikchange site-directed mutagenesis kit (Agilent) or by two-stage PCR with Q5 enzyme (New England Biolabs) using mutagenic DNA primers from Eurofins Operon and verified by DNA sequencing (Eton Bioscience, Charlestown, MA). .. Protein expression and purification WT OTC and variants were expressed in BL21 (DE3) Tuner cells (Novagen) in 1 L of Luria broth with 25 μg/mL chloramphenicol at 37°C.

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
    Article Snippet: .. First, we have generated an NGS dataset generated from a single PCR amplification using the Q5 enzyme (NEB), as previously described for the T2 experiment, of three different templates: (AC)20 , (AC)25 and (AC)30 , each using three serially diluted templates (by 10-fold each). ..

    Spectrophotometry:

    Article Title: Development of a cyanobacterial heterologous polyketide production platform
    Article Snippet: PCR amplifications were carried out with Q5 enzyme (New England BioLabs) according to the manufacturer’s instructions. .. Nucleic acid concentrations were measured with a UV-Vis spectrophotometer NanoDrop 2000c.

    Purification:

    Article Title: Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons
    Article Snippet: Briefly, genes were amplified by PCR using the Q5 enzyme (New England Biolabs) and assembled into plasmids for the generation of AAV vectors using Gibson Assembly (New England Biolabs). .. Correct clones for each vector were amplified in Stbl2 Escherichia coli (Invitrogen), and the DNA was purified in endotoxin-free conditions (Qiagen).

    Produced:

    Article Title: Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons
    Article Snippet: Briefly, genes were amplified by PCR using the Q5 enzyme (New England Biolabs) and assembled into plasmids for the generation of AAV vectors using Gibson Assembly (New England Biolabs). .. AAV2 pseudotyped with the AAV1 capsid were produced and purified.

    Sequencing:

    Article Title: Interferon-Dependent Induction of Clr-b during Mouse Cytomegalovirus Infection Protects Bystander Cells from Natural Killer Cells via NKR-P1B-Mediated Inhibition
    Article Snippet: PCR products were cloned into the luciferase vectors pGL3-Basic or pGL4.22 (Promega) and then sequenced (Macrogen Inc., South Korea, or TCAG Sequencing, Hospital for Sick Children, Toronto, ON, Canada). .. To overexpress IRF3/7/9, their respective coding sequences were PCR amplified from MCMV-infected NIH3T3 cDNA using the Q5 enzyme (New England Biolabs) and the primers listed in online supplementary Table .

    Article Title: The conserved RNA recognition motif and C3H1 domain of the Not4 ubiquitin ligase regulate in vivo ligase function
    Article Snippet: Cloning The wild-type NOT4 open reading frame was amplified as a C-terminal mono-FLAG fusion using Q5 enzyme (New England Biolabs) and cloned into the Xba1 and Eco RI digested plasmid p416ADH . .. Mutations were introduced using the QuikChange Mutagenesis kit from Agilent Technologies and confirmed by sequencing.

    Article Title: The conserved RNA recognition motif and C3H1 domain of the Not4 ubiquitin ligase regulate in vivo ligase function
    Article Snippet: The wild-type NOT4 open reading frame was amplified as a C-terminal mono-FLAG fusion using Q5 enzyme (New England Biolabs) and cloned into the Xba1 and Eco RI digested plasmid p416 ADH . .. Mutations were introduced using the QuikChange Mutagenesis kit from Agilent Technologies and confirmed by sequencing.

    Generated:

    Article Title: Interferon-Dependent Induction of Clr-b during Mouse Cytomegalovirus Infection Protects Bystander Cells from Natural Killer Cells via NKR-P1B-Mediated Inhibition
    Article Snippet: The mutated 500-bp promoter PCR product was generated by GeneSOE using the indicated primers (online suppl. .. To overexpress IRF3/7/9, their respective coding sequences were PCR amplified from MCMV-infected NIH3T3 cDNA using the Q5 enzyme (New England Biolabs) and the primers listed in online supplementary Table .

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
    Article Snippet: .. First, we have generated an NGS dataset generated from a single PCR amplification using the Q5 enzyme (NEB), as previously described for the T2 experiment, of three different templates: (AC)20 , (AC)25 and (AC)30 , each using three serially diluted templates (by 10-fold each). ..

    Luciferase:

    Article Title: Interferon-Dependent Induction of Clr-b during Mouse Cytomegalovirus Infection Protects Bystander Cells from Natural Killer Cells via NKR-P1B-Mediated Inhibition
    Article Snippet: PCR products were cloned into the luciferase vectors pGL3-Basic or pGL4.22 (Promega) and then sequenced (Macrogen Inc., South Korea, or TCAG Sequencing, Hospital for Sick Children, Toronto, ON, Canada). .. To overexpress IRF3/7/9, their respective coding sequences were PCR amplified from MCMV-infected NIH3T3 cDNA using the Q5 enzyme (New England Biolabs) and the primers listed in online supplementary Table .

    DNA Sequencing:

    Article Title: Probing remote residues important for catalysis in Escherichia coli ornithine transcarbamoylase
    Article Snippet: .. Variants were prepared with a Quikchange site-directed mutagenesis kit (Agilent) or by two-stage PCR with Q5 enzyme (New England Biolabs) using mutagenic DNA primers from Eurofins Operon and verified by DNA sequencing (Eton Bioscience, Charlestown, MA). .. Protein expression and purification WT OTC and variants were expressed in BL21 (DE3) Tuner cells (Novagen) in 1 L of Luria broth with 25 μg/mL chloramphenicol at 37°C.

    Expressing:

    Article Title: Probing remote residues important for catalysis in Escherichia coli ornithine transcarbamoylase
    Article Snippet: Site-directed mutagenesis A pCA24N plasmid expressing His-tagged OTC from E . coli argI gene was obtained from ASKA [ ]. .. Variants were prepared with a Quikchange site-directed mutagenesis kit (Agilent) or by two-stage PCR with Q5 enzyme (New England Biolabs) using mutagenic DNA primers from Eurofins Operon and verified by DNA sequencing (Eton Bioscience, Charlestown, MA).

    Modification:

    Article Title: Interferon-Dependent Induction of Clr-b during Mouse Cytomegalovirus Infection Protects Bystander Cells from Natural Killer Cells via NKR-P1B-Mediated Inhibition
    Article Snippet: The pGL4.22 reporter vector was modified to contain a puromycin resistance cassette. .. To overexpress IRF3/7/9, their respective coding sequences were PCR amplified from MCMV-infected NIH3T3 cDNA using the Q5 enzyme (New England Biolabs) and the primers listed in online supplementary Table .

    Recombinant:

    Article Title: Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons
    Article Snippet: Recombinant AAV Cre-GFP, AAV ΔCre-GFP, and AAV Cre-GFP-Ires-Nrf1 vectors used in this study were prepared by the Canadian neurophotonics platform viral vector core. .. Briefly, genes were amplified by PCR using the Q5 enzyme (New England Biolabs) and assembled into plasmids for the generation of AAV vectors using Gibson Assembly (New England Biolabs).

    other:

    Article Title: Short tandem repeat stutter model inferred from direct measurement of in vitro stutter noise
    Article Snippet: UltraII and Q5, both neck to neck second best in the number of simulated cycles category, an expected result as both enzymes are based essentially on the same Q5 enzyme with a different mix composition, emphasizing the robustness of the model.

    Plasmid Preparation:

    Article Title: Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons
    Article Snippet: Recombinant AAV Cre-GFP, AAV ΔCre-GFP, and AAV Cre-GFP-Ires-Nrf1 vectors used in this study were prepared by the Canadian neurophotonics platform viral vector core. .. Briefly, genes were amplified by PCR using the Q5 enzyme (New England Biolabs) and assembled into plasmids for the generation of AAV vectors using Gibson Assembly (New England Biolabs).

    Article Title: Development of a cyanobacterial heterologous polyketide production platform
    Article Snippet: Paragraph title: 2.2. General methods for vector and plasmid assembly ... PCR amplifications were carried out with Q5 enzyme (New England BioLabs) according to the manufacturer’s instructions.

    Article Title: Interferon-Dependent Induction of Clr-b during Mouse Cytomegalovirus Infection Protects Bystander Cells from Natural Killer Cells via NKR-P1B-Mediated Inhibition
    Article Snippet: Paragraph title: Vector Construction ... To overexpress IRF3/7/9, their respective coding sequences were PCR amplified from MCMV-infected NIH3T3 cDNA using the Q5 enzyme (New England Biolabs) and the primers listed in online supplementary Table .

    Article Title: Probing remote residues important for catalysis in Escherichia coli ornithine transcarbamoylase
    Article Snippet: Site-directed mutagenesis A pCA24N plasmid expressing His-tagged OTC from E . coli argI gene was obtained from ASKA [ ]. .. Variants were prepared with a Quikchange site-directed mutagenesis kit (Agilent) or by two-stage PCR with Q5 enzyme (New England Biolabs) using mutagenic DNA primers from Eurofins Operon and verified by DNA sequencing (Eton Bioscience, Charlestown, MA).

    Article Title: The conserved RNA recognition motif and C3H1 domain of the Not4 ubiquitin ligase regulate in vivo ligase function
    Article Snippet: .. Cloning The wild-type NOT4 open reading frame was amplified as a C-terminal mono-FLAG fusion using Q5 enzyme (New England Biolabs) and cloned into the Xba1 and Eco RI digested plasmid p416ADH . .. Mutations were introduced using the QuikChange Mutagenesis kit from Agilent Technologies and confirmed by sequencing.

    Article Title: The conserved RNA recognition motif and C3H1 domain of the Not4 ubiquitin ligase regulate in vivo ligase function
    Article Snippet: .. The wild-type NOT4 open reading frame was amplified as a C-terminal mono-FLAG fusion using Q5 enzyme (New England Biolabs) and cloned into the Xba1 and Eco RI digested plasmid p416 ADH . .. Mutations were introduced using the QuikChange Mutagenesis kit from Agilent Technologies and confirmed by sequencing.

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