q exactive hybrid quadrupole orbitrap mass spectrometer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher q exactive hybrid quadrupole orbitrap mass spectrometer
    Q Exactive Hybrid Quadrupole Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 467 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q exactive hybrid quadrupole orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 467 article reviews
    Price from $9.99 to $1999.99
    q exactive hybrid quadrupole orbitrap mass spectrometer - by Bioz Stars, 2020-03
    99/100 stars

    Images

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: 7-O-Esters of taxifolin with pronounced and overadditive effects in neuroprotection, anti-neuroinflammation, and amelioration of short-term memory impairment in vivo
    Article Snippet: The reaction mixture was fractionated by reversed-phase HPLC employing a linear gradient (0–80% acetonitrile in 0.1% trifluoroacetic acid within 40–60 min). .. Mass Spectrometry was performed using a ThermoFisher Q Exactive Hybrid Quadrupole Orbitrap mass spectrometer.

    Article Title: Fatty acid transporter 2 reprograms neutrophils in cancer
    Article Snippet: The Dionex HPLC system was coupled to an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher Scientific) or to a hybrid quadrupole-orbitrap mass spectrometer, Q-Exactive (ThermoFisher, Inc., San Jose, CA) with the Xcalibur operating system. .. MS and MS/MS analysis of free fatty acids (FFA) and TAG/CE was performed on a Q-Exactive hybrid-quadrupole-orbitrap mass spectrometer (ThermoFisher, Inc. San Jose, CA).

    Article Title: Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes
    Article Snippet: .. EASY-nLC 1000 or similar HPLC with autosampler connected to a Thermo-Fisher Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer, or any similar high-resolution, accurate-mass mass spectrometer. ..

    Article Title: Endoplasmic reticulum-derived bodies enable a single-cell chemical defense in Brassicaceae plants
    Article Snippet: Protein mass spectroscopy Samples were analyzed by nano-flow reverse-phase liquid chromatography followed by tandem MS, using a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific) as described . .. A capillary reverse-phase HPLC-MS/MS system was composed of a Dionex U3000 gradient pump equipped with a VICI CHEMINERT valve, and a Q Exactive equipped with a Dream Spray nano-electrospray ionization (NSI) source (AMR, Tokyo, Japan).

    Article Title: Electrophilic fatty acid nitroalkenes are systemically transported and distributed upon esterification to complex lipids
    Article Snippet: Paragraph title: HPLC-MS/MS analysis ... The 10-NO2 -OA-TAGs were eluted using the following gradient: 35–90% solvent B (0–10 min); 90% solvent B (10–13 min) to then reach the initial conditions in 0.5 min and re-equilibrate for an additional 1.5 min. A Q-Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Scientific) equipped with a HESI II electrospray source was used to characterize 10-NO2 -OA-TAG in positive mode.

    Hydrophilic Interaction Liquid Chromatography:

    Article Title: Plasma Acylcarnitines and Risk of Type 2 Diabetes in a Mediterranean Population at High Cardiovascular Risk
    Article Snippet: Briefly, data were acquired using a system comprised of a Shimadzu Nexera X2 ultra–high-performance liquid chromatograph coupled to a Q Exactive hybrid quadrupole orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). .. The samples were centrifuged (10 minutes, 9000 × g , 4°C) and the supernatant fluid was injected directly onto a 150-mm × 2-mm, 3-µm Atlantis hydrophilic interaction liquid chromatography column (Waters).

    Centrifugation:

    Article Title: Comparison of the molecular and cellular phenotypes of common mouse syngeneic models with human tumors
    Article Snippet: Following digestion, the peptides were captured by centrifugation at 15,000 g for 20 min, followed by adjusting the solution pH to acidic by adding 10% formic acid to the final concentration of 0.25% formic acid. .. Each sample was acquired on a Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer fitted with a Dionex nano liquid chromatography and EASY-Spray™ Ion source.

    Article Title: 7-O-Esters of taxifolin with pronounced and overadditive effects in neuroprotection, anti-neuroinflammation, and amelioration of short-term memory impairment in vivo
    Article Snippet: After sonication, cell debris was pelleted by centrifugation and the supernatant was collected for HPLC and MS analysis. .. Mass Spectrometry was performed using a ThermoFisher Q Exactive Hybrid Quadrupole Orbitrap mass spectrometer.

    Mass Spectrometry:

    Article Title: Hybrid Transcription Factor Engineering Activates the Silent Secondary Metabolite Gene Cluster for (+)-Asperlin in Aspergillus nidulans
    Article Snippet: .. High resolution electrospray ionization mass spectrum (HRESIMS) was obtained on ThermoScientific Q Exactive hybrid quadrupole-Orbitrap mass spectrometer with an Eclipse XDB-C18 column (Agilent 5 μm 4.6 × 150 mm) at a flow rate of 125 μL min−1 . .. Conditions for MS included a spray voltage of 3.5 kV, sheath gas flow rate 20 au, auxiliary gas flow rate at 5 au, sweep gas flow rate at 1 au, capillary temperature at 275 °C, s-lens RF level 55, auxiliary gas heat temperature at 325 °C, scan range of 100-600 m/z, resolution 140,000, AGC target 3 × 106 , and maximum injection time of 200 ms.

    Article Title: Comparison of the molecular and cellular phenotypes of common mouse syngeneic models with human tumors
    Article Snippet: .. Each sample was acquired on a Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer fitted with a Dionex nano liquid chromatography and EASY-Spray™ Ion source. .. The tryptic digest was loaded onto a reversed-phase pre-column (C18 trap column, Acclaim PepMap 100, 100 μm × 2 cm, Thermo Fisher Scientific, Inc.).

    Article Title: 7-O-Esters of taxifolin with pronounced and overadditive effects in neuroprotection, anti-neuroinflammation, and amelioration of short-term memory impairment in vivo
    Article Snippet: .. Mass Spectrometry was performed using a ThermoFisher Q Exactive Hybrid Quadrupole Orbitrap mass spectrometer. .. Samples were introduced by direct electrospray infusion of the HPLC fractions diluted with a one-to-one mixture of methanol and water containing 0.1% formic acid.

    Article Title: Effect of Oral Administration of Active Peptides of Pinctada Martensii on the Repair of Skin Wounds
    Article Snippet: .. Major Peptide Sequence Analysis of APs APs were identified using Thermo Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer with electrospray ionization (ESI) interface (Thermo Fisher Scientif, USA). .. The secondary mass spectrometry was based on the results of APs primary mass spectrometer total ion map.

    Article Title: Quantitative profiling of cortisol metabolites in human urine by high-resolution accurate-mass MS
    Article Snippet: .. Mass spectral data of each cortisol and cortisone metabolites were acquired by direct infusion with a Q Exactive Hybrid Quadrupole Orbitrap Mass Spectrometer (Thermo Fisher, CA, USA) and a Heated ESI (HESI-II) source (Thermo Fisher). .. The flow rate of direct infusion was set as 10 μl/min.

    Article Title: Fatty acid transporter 2 reprograms neutrophils in cancer
    Article Snippet: .. MS and MS/MS analysis of free fatty acids (FFA) and TAG/CE was performed on a Q-Exactive hybrid-quadrupole-orbitrap mass spectrometer (ThermoFisher, Inc. San Jose, CA). .. MS lipid standards were from Avanti Polar Lipids (Alabaster, AL) and from Cayman Chemical Company (Ann Arbor, MI).

    Article Title: CKAP4 Regulates Cell Migration via the Interaction with and Recycling of Integrin
    Article Snippet: .. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed as described previously ( ) using an UltiMate 3000 nano LC system (Thermo Fisher Scientific, Waltham, MA) coupled to a Q-Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) with a nanoelectrospray ionization source. .. Digested samples were injected using an autosampler and enriched on a C18 reverse-phase trap column (100-μm inside diameter by 5-mm length; Thermo Fisher Scientific) at a flow rate of 4 μl/min.

    Article Title: Glycosylation deficiency of lipopolysaccharide-binding protein and corticosteroid-binding globulin associated with activity and response to treatment for rheumatoid arthritis
    Article Snippet: .. The chromatography system was coupled to a Thermo Scientific Q Exactive Hybrid Quadrupole-orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany), programmed for data-dependent acquisition mode. .. The mass spectrometer method was a Top12 MS/MS method, meaning that the spectrometer acquired one full MS spectrum, selected the 12 most intense peaks in this spectrum (singly charged precursors excluded) and made a full MS2 spectrum of each of these 12 compounds.

    Article Title: Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes
    Article Snippet: .. EASY-nLC 1000 or similar HPLC with autosampler connected to a Thermo-Fisher Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer, or any similar high-resolution, accurate-mass mass spectrometer. ..

    Article Title: Endoplasmic reticulum-derived bodies enable a single-cell chemical defense in Brassicaceae plants
    Article Snippet: .. Protein mass spectroscopy Samples were analyzed by nano-flow reverse-phase liquid chromatography followed by tandem MS, using a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific) as described . .. A capillary reverse-phase HPLC-MS/MS system was composed of a Dionex U3000 gradient pump equipped with a VICI CHEMINERT valve, and a Q Exactive equipped with a Dream Spray nano-electrospray ionization (NSI) source (AMR, Tokyo, Japan).

    Article Title: Labeling of Phosphatidylinositol Lipid Products in Cells via Metabolic Engineering using a Clickable myo-Inositol Probe
    Article Snippet: .. Samples were analyzed by LCMS using a Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer. .. MS data were analysed using the MAVEN software package in order to search for a list of potential products expected from metabolic labeling of products using probe 1a .

    Article Title: Methoxymethyl Threofuranosyl Thymidine (4′-MOM-TNA-T) at the T7 Position of the Thrombin-Binding Aptamer Boosts Anticoagulation Activity, Thermal Stability, and Nuclease Resistance
    Article Snippet: .. Mass spectra were recorded on a Q Exactive Hybrid Quadrupole Orbitrap Mass spectrometer (Thermo Fisher Scientific), MALDI-TOF spectra were recorded on a SCIEX TOF/TOF 5800 system, and the matrix used for analysis was THAP (2,4,6-trihydroxyacetophenone)/ammonium citrate (2:1). .. UV experiments were carried out on a Varian Cary 300 UV-Visible spectrophotometer equipped with a Peltier-controlled cell holder.

    Article Title: Plasma Acylcarnitines and Risk of Type 2 Diabetes in a Mediterranean Population at High Cardiovascular Risk
    Article Snippet: .. Briefly, data were acquired using a system comprised of a Shimadzu Nexera X2 ultra–high-performance liquid chromatograph coupled to a Q Exactive hybrid quadrupole orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). .. Metabolite extracts were prepared from plasma samples (10 µL) via protein precipitation with the addition of 9 volumes of 74.9:24.9:0.2 (v/v/v) of acetonitrile/methanol/formic acid–containing stable isotope-labeled internal standards [valine-d8 (Sigma-Aldrich, St. Louis, MO) and phenylalanine-d8 (Cambridge Isotope Laboratories, Cambridge, MA)].

    Article Title: Electrophilic fatty acid nitroalkenes are systemically transported and distributed upon esterification to complex lipids
    Article Snippet: .. The 10-NO2 -OA-TAGs were eluted using the following gradient: 35–90% solvent B (0–10 min); 90% solvent B (10–13 min) to then reach the initial conditions in 0.5 min and re-equilibrate for an additional 1.5 min. A Q-Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Scientific) equipped with a HESI II electrospray source was used to characterize 10-NO2 -OA-TAG in positive mode. .. The following parameters were used: auxiliary gas heater temperature 250°C, capillary temperature 300°C, sheath gas flow rate 20, auxiliary gas flow rate 20, sweep gas flow rate 0, spray voltage 4 kV.

    Chromatography:

    Article Title: Fatty acid transporter 2 reprograms neutrophils in cancer
    Article Snippet: Paragraph title: Liquid chromatography-mass-spectrometry of lipids. ... MS and MS/MS analysis of free fatty acids (FFA) and TAG/CE was performed on a Q-Exactive hybrid-quadrupole-orbitrap mass spectrometer (ThermoFisher, Inc. San Jose, CA).

    Article Title: CKAP4 Regulates Cell Migration via the Interaction with and Recycling of Integrin
    Article Snippet: .. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed as described previously ( ) using an UltiMate 3000 nano LC system (Thermo Fisher Scientific, Waltham, MA) coupled to a Q-Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) with a nanoelectrospray ionization source. .. Digested samples were injected using an autosampler and enriched on a C18 reverse-phase trap column (100-μm inside diameter by 5-mm length; Thermo Fisher Scientific) at a flow rate of 4 μl/min.

    Article Title: Glycosylation deficiency of lipopolysaccharide-binding protein and corticosteroid-binding globulin associated with activity and response to treatment for rheumatoid arthritis
    Article Snippet: .. The chromatography system was coupled to a Thermo Scientific Q Exactive Hybrid Quadrupole-orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany), programmed for data-dependent acquisition mode. .. The mass spectrometer method was a Top12 MS/MS method, meaning that the spectrometer acquired one full MS spectrum, selected the 12 most intense peaks in this spectrum (singly charged precursors excluded) and made a full MS2 spectrum of each of these 12 compounds.

    Article Title: Plasma Acylcarnitines and Risk of Type 2 Diabetes in a Mediterranean Population at High Cardiovascular Risk
    Article Snippet: Liquid chromatography–tandem mass spectrometry (LC-MS) was used to quantitatively profile acylcarnitines in plasma samples. .. Briefly, data were acquired using a system comprised of a Shimadzu Nexera X2 ultra–high-performance liquid chromatograph coupled to a Q Exactive hybrid quadrupole orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA).

    Isolation:

    Article Title: Glycosylation deficiency of lipopolysaccharide-binding protein and corticosteroid-binding globulin associated with activity and response to treatment for rheumatoid arthritis
    Article Snippet: The chromatography system was coupled to a Thermo Scientific Q Exactive Hybrid Quadrupole-orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany), programmed for data-dependent acquisition mode. .. The parameters for MS/MS spectrum acquisition were: isolation window of 2.0 m/z, ion trap higher energy collision dissociation (HCD) fragmentation normalized collision energy (NCE) of 25, resolution of 17,500, AGC target of 1 × 105 or maximum injection time of 50 ms, dynamic exclusion: 10 s. Proteins were identified using MaxQuant v.1.5.5.1 and quantified using Label-Free Quantification determination [ ].

    Western Blot:

    Article Title: 7-O-Esters of taxifolin with pronounced and overadditive effects in neuroprotection, anti-neuroinflammation, and amelioration of short-term memory impairment in vivo
    Article Snippet: Paragraph title: Western blots ... Mass Spectrometry was performed using a ThermoFisher Q Exactive Hybrid Quadrupole Orbitrap mass spectrometer.

    Flow Cytometry:

    Article Title: Hybrid Transcription Factor Engineering Activates the Silent Secondary Metabolite Gene Cluster for (+)-Asperlin in Aspergillus nidulans
    Article Snippet: .. High resolution electrospray ionization mass spectrum (HRESIMS) was obtained on ThermoScientific Q Exactive hybrid quadrupole-Orbitrap mass spectrometer with an Eclipse XDB-C18 column (Agilent 5 μm 4.6 × 150 mm) at a flow rate of 125 μL min−1 . .. Conditions for MS included a spray voltage of 3.5 kV, sheath gas flow rate 20 au, auxiliary gas flow rate at 5 au, sweep gas flow rate at 1 au, capillary temperature at 275 °C, s-lens RF level 55, auxiliary gas heat temperature at 325 °C, scan range of 100-600 m/z, resolution 140,000, AGC target 3 × 106 , and maximum injection time of 200 ms.

    Article Title: Comparison of the molecular and cellular phenotypes of common mouse syngeneic models with human tumors
    Article Snippet: Each sample was acquired on a Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer fitted with a Dionex nano liquid chromatography and EASY-Spray™ Ion source. .. The gradient was comprised of an increase from 2 to 35% mobile phase B (0.1% formic acid in acetonitrile) over 160 min, followed by 35 to 80% B over 10 min and a hold at 80% B for the last 10 min, all at a fixed flow rate of 300 nl/min in an UltiMat 3000 RSLCnano system (Thermo Fisher Scientific, Inc.).

    Article Title: Quantitative profiling of cortisol metabolites in human urine by high-resolution accurate-mass MS
    Article Snippet: Mass spectral data of each cortisol and cortisone metabolites were acquired by direct infusion with a Q Exactive Hybrid Quadrupole Orbitrap Mass Spectrometer (Thermo Fisher, CA, USA) and a Heated ESI (HESI-II) source (Thermo Fisher). .. The flow rate of direct infusion was set as 10 μl/min.

    Article Title: CKAP4 Regulates Cell Migration via the Interaction with and Recycling of Integrin
    Article Snippet: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed as described previously ( ) using an UltiMate 3000 nano LC system (Thermo Fisher Scientific, Waltham, MA) coupled to a Q-Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) with a nanoelectrospray ionization source. .. Digested samples were injected using an autosampler and enriched on a C18 reverse-phase trap column (100-μm inside diameter by 5-mm length; Thermo Fisher Scientific) at a flow rate of 4 μl/min.

    Article Title: Glycosylation deficiency of lipopolysaccharide-binding protein and corticosteroid-binding globulin associated with activity and response to treatment for rheumatoid arthritis
    Article Snippet: Each eluted fraction was desalted on the trap column after a ten times online dilution to pH 3 and subsequently separated on the analytical column; flow rate 250 nL/min, solvent A (0.1% formic acid in water) and solvent B (0.1% formic acid in acetonitrile) with a linear gradient: 0 min, 99% A; 5 min, 93% A; 140 min, 65% A. .. The chromatography system was coupled to a Thermo Scientific Q Exactive Hybrid Quadrupole-orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany), programmed for data-dependent acquisition mode.

    Article Title: Plasma Acylcarnitines and Risk of Type 2 Diabetes in a Mediterranean Population at High Cardiovascular Risk
    Article Snippet: Briefly, data were acquired using a system comprised of a Shimadzu Nexera X2 ultra–high-performance liquid chromatograph coupled to a Q Exactive hybrid quadrupole orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). .. The column was eluted isocratically at a flow rate of 250 µL/min with 5% mobile phase A (10 mmol ammonium formate/L and 0.1% formic acid in water) for 0.5 minutes followed by a linear gradient to 40% mobile phase B (acetonitrile with 0.1% formic acid) over 10 minutes.

    Article Title: Electrophilic fatty acid nitroalkenes are systemically transported and distributed upon esterification to complex lipids
    Article Snippet: Analysis of 10-NO2 -OA-TAG was performed by HPLC-HR-MS/MS using a C18 Luna column (2 × 150 mm, 3 μm; Phenomenex) at a flow rate of 0.4 ml/min with a post column infusion of 50 μl/min of 10% ammonium acetate in acetonitrile (10 mM final). .. The 10-NO2 -OA-TAGs were eluted using the following gradient: 35–90% solvent B (0–10 min); 90% solvent B (10–13 min) to then reach the initial conditions in 0.5 min and re-equilibrate for an additional 1.5 min. A Q-Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Scientific) equipped with a HESI II electrospray source was used to characterize 10-NO2 -OA-TAG in positive mode.

    Nuclear Magnetic Resonance:

    Article Title: Hybrid Transcription Factor Engineering Activates the Silent Secondary Metabolite Gene Cluster for (+)-Asperlin in Aspergillus nidulans
    Article Snippet: NMR spectral data were collected on a Varian Mercury Plus 400 spectrometer. .. High resolution electrospray ionization mass spectrum (HRESIMS) was obtained on ThermoScientific Q Exactive hybrid quadrupole-Orbitrap mass spectrometer with an Eclipse XDB-C18 column (Agilent 5 μm 4.6 × 150 mm) at a flow rate of 125 μL min−1 .

    Article Title: Methoxymethyl Threofuranosyl Thymidine (4′-MOM-TNA-T) at the T7 Position of the Thrombin-Binding Aptamer Boosts Anticoagulation Activity, Thermal Stability, and Nuclease Resistance
    Article Snippet: The chemical shifts are referred to internal TMS for 1 H and chloroform-d for 13 C NMR. .. Mass spectra were recorded on a Q Exactive Hybrid Quadrupole Orbitrap Mass spectrometer (Thermo Fisher Scientific), MALDI-TOF spectra were recorded on a SCIEX TOF/TOF 5800 system, and the matrix used for analysis was THAP (2,4,6-trihydroxyacetophenone)/ammonium citrate (2:1).

    Concentration Assay:

    Article Title: Comparison of the molecular and cellular phenotypes of common mouse syngeneic models with human tumors
    Article Snippet: Following digestion, the peptides were captured by centrifugation at 15,000 g for 20 min, followed by adjusting the solution pH to acidic by adding 10% formic acid to the final concentration of 0.25% formic acid. .. Each sample was acquired on a Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer fitted with a Dionex nano liquid chromatography and EASY-Spray™ Ion source.

    Incubation:

    Article Title: 7-O-Esters of taxifolin with pronounced and overadditive effects in neuroprotection, anti-neuroinflammation, and amelioration of short-term memory impairment in vivo
    Article Snippet: Cells were incubated for the indicated time periods after which they were scraped and transferred to Eppendorf tubes. .. Mass Spectrometry was performed using a ThermoFisher Q Exactive Hybrid Quadrupole Orbitrap mass spectrometer.

    Thin Layer Chromatography:

    Article Title: Labeling of Phosphatidylinositol Lipid Products in Cells via Metabolic Engineering using a Clickable myo-Inositol Probe
    Article Snippet: For MS analysis, TLC plates prepared as described above were scraped and extracted 2 times with 2 mL of chloroform/methanol/conc. .. Samples were analyzed by LCMS using a Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer.

    Article Title: Methoxymethyl Threofuranosyl Thymidine (4′-MOM-TNA-T) at the T7 Position of the Thrombin-Binding Aptamer Boosts Anticoagulation Activity, Thermal Stability, and Nuclease Resistance
    Article Snippet: Mass spectra were recorded on a Q Exactive Hybrid Quadrupole Orbitrap Mass spectrometer (Thermo Fisher Scientific), MALDI-TOF spectra were recorded on a SCIEX TOF/TOF 5800 system, and the matrix used for analysis was THAP (2,4,6-trihydroxyacetophenone)/ammonium citrate (2:1). .. Mass spectra were recorded on a Q Exactive Hybrid Quadrupole Orbitrap Mass spectrometer (Thermo Fisher Scientific), MALDI-TOF spectra were recorded on a SCIEX TOF/TOF 5800 system, and the matrix used for analysis was THAP (2,4,6-trihydroxyacetophenone)/ammonium citrate (2:1).

    Labeling:

    Article Title: Labeling of Phosphatidylinositol Lipid Products in Cells via Metabolic Engineering using a Clickable myo-Inositol Probe
    Article Snippet: Samples were analyzed by LCMS using a Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer. .. MS data were analysed using the MAVEN software package in order to search for a list of potential products expected from metabolic labeling of products using probe 1a .

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Fatty acid transporter 2 reprograms neutrophils in cancer
    Article Snippet: MS and MS/MS analysis of free fatty acids (FFA) and TAG/CE was performed on a Q-Exactive hybrid-quadrupole-orbitrap mass spectrometer (ThermoFisher, Inc. San Jose, CA). .. Analysis of LC-MS data was performed using the software package Compound Discoverer™ (ThemoFisher Scientific).

    Article Title: Labeling of Phosphatidylinositol Lipid Products in Cells via Metabolic Engineering using a Clickable myo-Inositol Probe
    Article Snippet: .. Samples were analyzed by LCMS using a Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer. .. MS data were analysed using the MAVEN software package in order to search for a list of potential products expected from metabolic labeling of products using probe 1a .

    Article Title: Plasma Acylcarnitines and Risk of Type 2 Diabetes in a Mediterranean Population at High Cardiovascular Risk
    Article Snippet: Details of the LC-MS platform can be found elsewhere ( ). .. Briefly, data were acquired using a system comprised of a Shimadzu Nexera X2 ultra–high-performance liquid chromatograph coupled to a Q Exactive hybrid quadrupole orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA).

    Tandem Mass Spectroscopy:

    Article Title: 7-O-Esters of taxifolin with pronounced and overadditive effects in neuroprotection, anti-neuroinflammation, and amelioration of short-term memory impairment in vivo
    Article Snippet: Mass Spectrometry was performed using a ThermoFisher Q Exactive Hybrid Quadrupole Orbitrap mass spectrometer. .. MS/MS fragmentation was performed on the major peaks which did not correspond with background signals.

    Article Title: Fatty acid transporter 2 reprograms neutrophils in cancer
    Article Snippet: .. MS and MS/MS analysis of free fatty acids (FFA) and TAG/CE was performed on a Q-Exactive hybrid-quadrupole-orbitrap mass spectrometer (ThermoFisher, Inc. San Jose, CA). .. MS lipid standards were from Avanti Polar Lipids (Alabaster, AL) and from Cayman Chemical Company (Ann Arbor, MI).

    Article Title: Glycosylation deficiency of lipopolysaccharide-binding protein and corticosteroid-binding globulin associated with activity and response to treatment for rheumatoid arthritis
    Article Snippet: The chromatography system was coupled to a Thermo Scientific Q Exactive Hybrid Quadrupole-orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany), programmed for data-dependent acquisition mode. .. The mass spectrometer method was a Top12 MS/MS method, meaning that the spectrometer acquired one full MS spectrum, selected the 12 most intense peaks in this spectrum (singly charged precursors excluded) and made a full MS2 spectrum of each of these 12 compounds.

    Sequencing:

    Article Title: Effect of Oral Administration of Active Peptides of Pinctada Martensii on the Repair of Skin Wounds
    Article Snippet: .. Major Peptide Sequence Analysis of APs APs were identified using Thermo Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer with electrospray ionization (ESI) interface (Thermo Fisher Scientif, USA). .. The secondary mass spectrometry was based on the results of APs primary mass spectrometer total ion map.

    Sonication:

    Article Title: 7-O-Esters of taxifolin with pronounced and overadditive effects in neuroprotection, anti-neuroinflammation, and amelioration of short-term memory impairment in vivo
    Article Snippet: After sonication, cell debris was pelleted by centrifugation and the supernatant was collected for HPLC and MS analysis. .. Mass Spectrometry was performed using a ThermoFisher Q Exactive Hybrid Quadrupole Orbitrap mass spectrometer.

    Article Title: Labeling of Phosphatidylinositol Lipid Products in Cells via Metabolic Engineering using a Clickable myo-Inositol Probe
    Article Snippet: HCl (20/40/1, v/v/v) with the help of sonication and vortexing. .. Samples were analyzed by LCMS using a Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer.

    Injection:

    Article Title: Hybrid Transcription Factor Engineering Activates the Silent Secondary Metabolite Gene Cluster for (+)-Asperlin in Aspergillus nidulans
    Article Snippet: High resolution electrospray ionization mass spectrum (HRESIMS) was obtained on ThermoScientific Q Exactive hybrid quadrupole-Orbitrap mass spectrometer with an Eclipse XDB-C18 column (Agilent 5 μm 4.6 × 150 mm) at a flow rate of 125 μL min−1 . .. Conditions for MS included a spray voltage of 3.5 kV, sheath gas flow rate 20 au, auxiliary gas flow rate at 5 au, sweep gas flow rate at 1 au, capillary temperature at 275 °C, s-lens RF level 55, auxiliary gas heat temperature at 325 °C, scan range of 100-600 m/z, resolution 140,000, AGC target 3 × 106 , and maximum injection time of 200 ms.

    Article Title: Quantitative profiling of cortisol metabolites in human urine by high-resolution accurate-mass MS
    Article Snippet: Mass spectral data of each cortisol and cortisone metabolites were acquired by direct infusion with a Q Exactive Hybrid Quadrupole Orbitrap Mass Spectrometer (Thermo Fisher, CA, USA) and a Heated ESI (HESI-II) source (Thermo Fisher). .. Instrument tuning parameters were as follows: sheath gas, 5; auxiliary gas, 0; auxiliary gas temperature, 50°C; capillary temperature, 300°C; spray voltage, 4 kV; 1μ scan; maximum injection time, 100 and 200 ms for full MS and MSn , respectively; in source collision-induced dissociation, 10 eV; and higher-energy collisional dissociation (HCD), 20 for MS2 .

    Article Title: CKAP4 Regulates Cell Migration via the Interaction with and Recycling of Integrin
    Article Snippet: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed as described previously ( ) using an UltiMate 3000 nano LC system (Thermo Fisher Scientific, Waltham, MA) coupled to a Q-Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) with a nanoelectrospray ionization source. .. Digested samples were injected using an autosampler and enriched on a C18 reverse-phase trap column (100-μm inside diameter by 5-mm length; Thermo Fisher Scientific) at a flow rate of 4 μl/min.

    Article Title: Glycosylation deficiency of lipopolysaccharide-binding protein and corticosteroid-binding globulin associated with activity and response to treatment for rheumatoid arthritis
    Article Snippet: The chromatography system was coupled to a Thermo Scientific Q Exactive Hybrid Quadrupole-orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany), programmed for data-dependent acquisition mode. .. The parameters for MS spectrum acquisition were: mass range from 400 to 1750 m/z, resolution of 70,000 (full width at half maximum), AGC target of 1 × 106 or maximum injection time of 200 ms.

    Article Title: Endoplasmic reticulum-derived bodies enable a single-cell chemical defense in Brassicaceae plants
    Article Snippet: Protein mass spectroscopy Samples were analyzed by nano-flow reverse-phase liquid chromatography followed by tandem MS, using a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific) as described . .. Samples were automatically injected using a PAL System autosampler (CTC Analytics, Zwingen, Switzerland) and a peptide L-trap column (Trap and Elute mode, Chemical Evaluation Research Institute, Tokyo) attached to an injector valve for desalinating and concentrating peptides.

    Article Title: Plasma Acylcarnitines and Risk of Type 2 Diabetes in a Mediterranean Population at High Cardiovascular Risk
    Article Snippet: Briefly, data were acquired using a system comprised of a Shimadzu Nexera X2 ultra–high-performance liquid chromatograph coupled to a Q Exactive hybrid quadrupole orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). .. The samples were centrifuged (10 minutes, 9000 × g , 4°C) and the supernatant fluid was injected directly onto a 150-mm × 2-mm, 3-µm Atlantis hydrophilic interaction liquid chromatography column (Waters).

    Spectrophotometry:

    Article Title: Methoxymethyl Threofuranosyl Thymidine (4′-MOM-TNA-T) at the T7 Position of the Thrombin-Binding Aptamer Boosts Anticoagulation Activity, Thermal Stability, and Nuclease Resistance
    Article Snippet: Mass spectra were recorded on a Q Exactive Hybrid Quadrupole Orbitrap Mass spectrometer (Thermo Fisher Scientific), MALDI-TOF spectra were recorded on a SCIEX TOF/TOF 5800 system, and the matrix used for analysis was THAP (2,4,6-trihydroxyacetophenone)/ammonium citrate (2:1). .. UV experiments were carried out on a Varian Cary 300 UV-Visible spectrophotometer equipped with a Peltier-controlled cell holder.

    Liquid Chromatography:

    Article Title: Comparison of the molecular and cellular phenotypes of common mouse syngeneic models with human tumors
    Article Snippet: .. Each sample was acquired on a Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer fitted with a Dionex nano liquid chromatography and EASY-Spray™ Ion source. .. The tryptic digest was loaded onto a reversed-phase pre-column (C18 trap column, Acclaim PepMap 100, 100 μm × 2 cm, Thermo Fisher Scientific, Inc.).

    Article Title: Endoplasmic reticulum-derived bodies enable a single-cell chemical defense in Brassicaceae plants
    Article Snippet: .. Protein mass spectroscopy Samples were analyzed by nano-flow reverse-phase liquid chromatography followed by tandem MS, using a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific) as described . .. A capillary reverse-phase HPLC-MS/MS system was composed of a Dionex U3000 gradient pump equipped with a VICI CHEMINERT valve, and a Q Exactive equipped with a Dream Spray nano-electrospray ionization (NSI) source (AMR, Tokyo, Japan).

    Software:

    Article Title: Fatty acid transporter 2 reprograms neutrophils in cancer
    Article Snippet: LC/ESI-MS analysis of lipids was performed on a Dionex HPLC system (utilizing the Chromeleon software), consisting of a Dionex UltiMate 3000 mobile phase pump, equipped with an UltiMate 3000 degassing unit and UltiMate 3000 autosampler (sampler chamber temperature was set at 4°C). .. MS and MS/MS analysis of free fatty acids (FFA) and TAG/CE was performed on a Q-Exactive hybrid-quadrupole-orbitrap mass spectrometer (ThermoFisher, Inc. San Jose, CA).

    Article Title: Labeling of Phosphatidylinositol Lipid Products in Cells via Metabolic Engineering using a Clickable myo-Inositol Probe
    Article Snippet: Samples were analyzed by LCMS using a Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer. .. MS data were analysed using the MAVEN software package in order to search for a list of potential products expected from metabolic labeling of products using probe 1a .

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 75
    Thermo Fisher q exactiveã¢â„⢠hybrid quadrupole orbitrapã¢â„⢠mass spectrometer
    Q Exactiveã¢â„⢠Hybrid Quadrupole Orbitrapã¢â„⢠Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q exactiveã¢â„⢠hybrid quadrupole orbitrapã¢â„⢠mass spectrometer/product/Thermo Fisher
    Average 75 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    q exactiveã¢â„⢠hybrid quadrupole orbitrapã¢â„⢠mass spectrometer - by Bioz Stars, 2020-03
    75/100 stars
      Buy from Supplier

    Image Search Results