px601 aav cmv nls sacas9 nls 3xha bghpa u6 bsai sgrna plasmid 61591  (Addgene inc)

 
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    Addgene inc px601 aav cmv nls sacas9 nls 3xha bghpa u6 bsai sgrna plasmid 61591
    <t>CRISPR/SaCas9–mediated</t> disruption of Kcnq2/3 genes in Hcrt neurons leads to NREM sleep fragmentation in young mice. ( A ) Schematic of <t>AAV</t> sgControl, AAV SaCas9, AAV sgKcnq2/3 vector design, and bilateral viral infection of Hcrt neurons in young Hcrt∷Cre mice. ( B ) Two-hour (left) binned percentage, (middle left) bout counts, (middle right) mean bout length and (right) mean bout length based on circadian phase for wake, NREM, and REM sleep at 1 week (top) and 8 weeks (bottom) after injection of a virus mixture, as illustrated in (A) ( n = 10 mice/group, dark phase indicated by gray shielding). ( C ) Representative slices expressing <t>sgRNA</t> with fluorescent mCherry flag and SaCas9–3HA for sgControl and sgKcnq2/3 group, respectively. Patch clamp recorded cells were labeled with biocytin, and post hoc antibody staining against HA tag confirmed the cells expressing SaCas9 for data analyses. ( D ) Comparison of RMPs between sgControl and sgKcnq2/3 group ( n = 14 neurons from three mice each group). ( E ) Fractions of neurons with different firing frequencies in the (left) sgControl and (right) sgKcnq2/3 groups. Data indicate mean ± SEM [(B) left to middle right, two-way RM ANOVA followed by Šidák’s multiple comparisons; (B) right, Holm-Šidák; (D) Mann-Whitney U test; * P
    Px601 Aav Cmv Nls Sacas9 Nls 3xha Bghpa U6 Bsai Sgrna Plasmid 61591, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/px601 aav cmv nls sacas9 nls 3xha bghpa u6 bsai sgrna plasmid 61591/product/Addgene inc
    Average 86 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    px601 aav cmv nls sacas9 nls 3xha bghpa u6 bsai sgrna plasmid 61591 - by Bioz Stars, 2022-11
    86/100 stars

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    1) Product Images from "Hyperexcitable arousal circuits drive sleep instability during aging"

    Article Title: Hyperexcitable arousal circuits drive sleep instability during aging

    Journal: Science (New York, N.Y.)

    doi: 10.1126/science.abh3021

    CRISPR/SaCas9–mediated disruption of Kcnq2/3 genes in Hcrt neurons leads to NREM sleep fragmentation in young mice. ( A ) Schematic of AAV sgControl, AAV SaCas9, AAV sgKcnq2/3 vector design, and bilateral viral infection of Hcrt neurons in young Hcrt∷Cre mice. ( B ) Two-hour (left) binned percentage, (middle left) bout counts, (middle right) mean bout length and (right) mean bout length based on circadian phase for wake, NREM, and REM sleep at 1 week (top) and 8 weeks (bottom) after injection of a virus mixture, as illustrated in (A) ( n = 10 mice/group, dark phase indicated by gray shielding). ( C ) Representative slices expressing sgRNA with fluorescent mCherry flag and SaCas9–3HA for sgControl and sgKcnq2/3 group, respectively. Patch clamp recorded cells were labeled with biocytin, and post hoc antibody staining against HA tag confirmed the cells expressing SaCas9 for data analyses. ( D ) Comparison of RMPs between sgControl and sgKcnq2/3 group ( n = 14 neurons from three mice each group). ( E ) Fractions of neurons with different firing frequencies in the (left) sgControl and (right) sgKcnq2/3 groups. Data indicate mean ± SEM [(B) left to middle right, two-way RM ANOVA followed by Šidák’s multiple comparisons; (B) right, Holm-Šidák; (D) Mann-Whitney U test; * P
    Figure Legend Snippet: CRISPR/SaCas9–mediated disruption of Kcnq2/3 genes in Hcrt neurons leads to NREM sleep fragmentation in young mice. ( A ) Schematic of AAV sgControl, AAV SaCas9, AAV sgKcnq2/3 vector design, and bilateral viral infection of Hcrt neurons in young Hcrt∷Cre mice. ( B ) Two-hour (left) binned percentage, (middle left) bout counts, (middle right) mean bout length and (right) mean bout length based on circadian phase for wake, NREM, and REM sleep at 1 week (top) and 8 weeks (bottom) after injection of a virus mixture, as illustrated in (A) ( n = 10 mice/group, dark phase indicated by gray shielding). ( C ) Representative slices expressing sgRNA with fluorescent mCherry flag and SaCas9–3HA for sgControl and sgKcnq2/3 group, respectively. Patch clamp recorded cells were labeled with biocytin, and post hoc antibody staining against HA tag confirmed the cells expressing SaCas9 for data analyses. ( D ) Comparison of RMPs between sgControl and sgKcnq2/3 group ( n = 14 neurons from three mice each group). ( E ) Fractions of neurons with different firing frequencies in the (left) sgControl and (right) sgKcnq2/3 groups. Data indicate mean ± SEM [(B) left to middle right, two-way RM ANOVA followed by Šidák’s multiple comparisons; (B) right, Holm-Šidák; (D) Mann-Whitney U test; * P

    Techniques Used: CRISPR, Mouse Assay, Plasmid Preparation, Infection, Injection, Expressing, Patch Clamp, Labeling, Staining, MANN-WHITNEY

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    Addgene inc px601 aav cmv nls sacas9 nls 3xha bghpa u6 bsai sgrna plasmid 61591
    Px601 Aav Cmv Nls Sacas9 Nls 3xha Bghpa U6 Bsai Sgrna Plasmid 61591, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/px601 aav cmv nls sacas9 nls 3xha bghpa u6 bsai sgrna plasmid 61591/product/Addgene inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    px601 aav cmv nls sacas9 nls 3xha bghpa u6 bsai sgrna plasmid 61591 - by Bioz Stars, 2022-11
    86/100 stars
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