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Merck & Co pvdf membrane immobilon p
Pvdf Membrane Immobilon P, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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pvdf membrane immobilon p - by Bioz Stars, 2020-11
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Article Title: Structure and functional implications of WYL domain-containing bacterial DNA damage response regulator PafBC
Article Snippet: Equal amounts of total protein were separated on a 10% SDS-containing polyacrylamide gel and transferred onto PVDF membrane Immobilon-P (Merck) using a BioRad Trans-Blot SD semidry transfer cell (20 V, maximum of 10 mA/cm2 , 30 min).

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    Merck & Co pvdf membranes
    Binding region of Fhb to <t>C3b/C3d.</t> A and B , ligand blotting analysis in which 20 μg of truncated Fhb constructs were run on SDS-polyacrylamide gels, transferred to <t>PVDF</t> membranes, and incubated with 5 μg/ml biotinylated C3b/C3d, and then
    Pvdf Membranes, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pvdf membranes/product/Merck & Co
    Average 92 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    pvdf membranes - by Bioz Stars, 2020-11
    92/100 stars
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    Binding region of Fhb to C3b/C3d. A and B , ligand blotting analysis in which 20 μg of truncated Fhb constructs were run on SDS-polyacrylamide gels, transferred to PVDF membranes, and incubated with 5 μg/ml biotinylated C3b/C3d, and then

    Journal: The Journal of Biological Chemistry

    Article Title: Mechanisms of Host-Pathogen Protein Complex Formation and Bacterial Immune Evasion of Streptococcus suis Protein Fhb *

    doi: 10.1074/jbc.M116.719443

    Figure Lengend Snippet: Binding region of Fhb to C3b/C3d. A and B , ligand blotting analysis in which 20 μg of truncated Fhb constructs were run on SDS-polyacrylamide gels, transferred to PVDF membranes, and incubated with 5 μg/ml biotinylated C3b/C3d, and then

    Article Snippet: Bands were detected using ECL Western blotting detection reagents (Thermo Scientific) and exposure to x-ray film (Fuji) at room temperature for 120 s. For the C3b/C3d binding assay, the PVDF membranes were probed with 10 μg/ml biotinylated C3b/C3d (Merck) for 1 h at room temperature, washed three times with TBST, and incubated with HRP-conjugated streptavidin (1:8000; GE Healthcare).

    Techniques: Binding Assay, Construct, Incubation

    SsaP and autocleavage of SsaU are essential for secretion of SseB and SseJ-2HA. (A) Levels of SseB, SseJ-2HA, and SsaI in whole bacteria and secreted fractions of different strains grown in magnesium minimal medium-morpholineethanesulfonic acid (MgM-MES) at pH 5.0. (B) The cleavage pattern of SsaU-6his and SsaU N262A -6his. Whole-bacterium lysates were separated by SDS-PAGE, transferred to a PVDF membrane, and probed with an anti-His antibody. Asterisks (*) indicate nonspecific bands. (C) Bacterial strains were grown in MgM-MES at pH 5.0 for 4 h, which was then changed to fresh MgM-MES at pH 5.0 or 7.2 as indicated, and the strains were then incubated for 1.5 h before analysis.

    Journal: mBio

    Article Title: SsaV Interacts with SsaL to Control the Translocon-to-Effector Switch in the Salmonella SPI-2 Type Three Secretion System

    doi: 10.1128/mBio.01149-18

    Figure Lengend Snippet: SsaP and autocleavage of SsaU are essential for secretion of SseB and SseJ-2HA. (A) Levels of SseB, SseJ-2HA, and SsaI in whole bacteria and secreted fractions of different strains grown in magnesium minimal medium-morpholineethanesulfonic acid (MgM-MES) at pH 5.0. (B) The cleavage pattern of SsaU-6his and SsaU N262A -6his. Whole-bacterium lysates were separated by SDS-PAGE, transferred to a PVDF membrane, and probed with an anti-His antibody. Asterisks (*) indicate nonspecific bands. (C) Bacterial strains were grown in MgM-MES at pH 5.0 for 4 h, which was then changed to fresh MgM-MES at pH 5.0 or 7.2 as indicated, and the strains were then incubated for 1.5 h before analysis.

    Article Snippet: Protein samples were separated through 12% SDS-polyacrylamide gels and transferred to Immobilon-P polyvinylidene difluoride (PVDF) membranes (Merck) for immunoblotting.

    Techniques: SDS Page, Incubation

    SsaP and autocleavage of SsaU are essential for secretion of SseB and SseJ-2HA. (A) Levels of SseB, SseJ-2HA, and SsaI in whole bacteria and secreted fractions of different strains grown in magnesium minimal medium-morpholineethanesulfonic acid (MgM-MES) at pH 5.0. (B) The cleavage pattern of SsaU-6his and SsaU N262A -6his. Whole-bacterium lysates were separated by SDS-PAGE, transferred to a PVDF membrane, and probed with an anti-His antibody. Asterisks (*) indicate nonspecific bands. (C) Bacterial strains were grown in MgM-MES at pH 5.0 for 4 h, which was then changed to fresh MgM-MES at pH 5.0 or 7.2 as indicated, and the strains were then incubated for 1.5 h before analysis.

    Journal: mBio

    Article Title: SsaV Interacts with SsaL to Control the Translocon-to-Effector Switch in the Salmonella SPI-2 Type Three Secretion System

    doi: 10.1128/mBio.01149-18

    Figure Lengend Snippet: SsaP and autocleavage of SsaU are essential for secretion of SseB and SseJ-2HA. (A) Levels of SseB, SseJ-2HA, and SsaI in whole bacteria and secreted fractions of different strains grown in magnesium minimal medium-morpholineethanesulfonic acid (MgM-MES) at pH 5.0. (B) The cleavage pattern of SsaU-6his and SsaU N262A -6his. Whole-bacterium lysates were separated by SDS-PAGE, transferred to a PVDF membrane, and probed with an anti-His antibody. Asterisks (*) indicate nonspecific bands. (C) Bacterial strains were grown in MgM-MES at pH 5.0 for 4 h, which was then changed to fresh MgM-MES at pH 5.0 or 7.2 as indicated, and the strains were then incubated for 1.5 h before analysis.

    Article Snippet: Protein samples were separated through 12% SDS-polyacrylamide gels and transferred to Immobilon-P polyvinylidene difluoride (PVDF) membranes (Merck) for immunoblotting.

    Techniques: SDS Page, Incubation