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GE Healthcare purification columns histrap crude ff
Purification Columns Histrap Crude Ff, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/purification columns histrap crude ff/product/GE Healthcare
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
purification columns histrap crude ff - by Bioz Stars, 2020-09
99/100 stars

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Centrifugation:

Article Title: A Mutation in CABP2, Expressed in Cochlear Hair Cells, Causes Autosomal-Recessive Hearing Impairment
Article Snippet: .. After centrifugation at 21,280 g, the His6 -tagged proteins were affinity purified from the supernatant with a HisTrap FF crude column (GE Healthcare, Munich, Germany). .. The proteins were eluted from the column, and the His6-tag was cleaved by the incubation of the protein solution with 1 U thrombin per 1 ml protein solution overnight at 4°C.

Affinity Chromatography:

Article Title: Screening, large-scale production, and structure-based classification for cystine-dense peptides
Article Snippet: .. CDP fusion proteins were purified by immobilized metal affinity chromatography (IMAC) with HisTrap FF Crude columns (GE) on an ÄKTA Pure FPLC system (GE). .. The CDP was cleaved from the Scn fusion partner by protease digestion, separated from unwanted digestion products by RPC (0.1% w/w TFA in water vs. 0.1% w/w TFA in acetonitrile) on a Tricorn 10/150 column packed with Source 15RPC resin (GE) using an ÄKTA Pure FPLC system, and lyophilized for storage.

Article Title: Recombinant Envelope-Proteins with Mutations in the Conserved Fusion Loop Allow Specific Serological Diagnosis of Dengue-Infections
Article Snippet: .. The DENV E proteins were purified by immobilized metal affinity chromatography (IMAC) with 5 ml HisTrap FF crude columns (GeHealthcare) and size exclusion chromatography with a 16/600 HiLoad Superdex 200 pg column (GeHealthcare) using the ÄKTA pure 25 l chromatography system (GeHealthcare). .. Purified proteins were quantified using a BCA protein assay (Pierce).

Chromatography:

Article Title: Recombinant Envelope-Proteins with Mutations in the Conserved Fusion Loop Allow Specific Serological Diagnosis of Dengue-Infections
Article Snippet: .. The DENV E proteins were purified by immobilized metal affinity chromatography (IMAC) with 5 ml HisTrap FF crude columns (GeHealthcare) and size exclusion chromatography with a 16/600 HiLoad Superdex 200 pg column (GeHealthcare) using the ÄKTA pure 25 l chromatography system (GeHealthcare). .. Purified proteins were quantified using a BCA protein assay (Pierce).

Protease Inhibitor:

Article Title: G-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A
Article Snippet: .. The bacteria were lysed in Buffer A (50 mM sodium dihydrogen phosphate pH 7.5, 0.2 M NaCl, 5 mM Imidazole, 1 × EDTA free protease inhibitor cocktail (Roche 04693132001) and 0.4 mM betamercaptoetanol) and purified on a crude HisTrap column (GE Healthcare 11-0004-58) using Buffer A and Buffer B (50 mM sodium dihydrogen phosphate pH 7.5, 0.2 M NaCl, 500 mM Imidazole, 1 × EDTA free protease inhibitor cocktail (Roche 04693132001) and 0.4 mM betamercaptoetanol). .. Fractions with the highest optical density at 280 nm were pooled, dialyzed to Buffer C (20 mM Tris-HCl, pH 8) and further purified by ion exchange chromatography (Resource Q, GE Healthcare 520348) at pH 8 with buffer C and buffer D (20 mM Tris-HCl pH 8, 1 M NaCl).

Size-exclusion Chromatography:

Article Title: Recombinant Envelope-Proteins with Mutations in the Conserved Fusion Loop Allow Specific Serological Diagnosis of Dengue-Infections
Article Snippet: .. The DENV E proteins were purified by immobilized metal affinity chromatography (IMAC) with 5 ml HisTrap FF crude columns (GeHealthcare) and size exclusion chromatography with a 16/600 HiLoad Superdex 200 pg column (GeHealthcare) using the ÄKTA pure 25 l chromatography system (GeHealthcare). .. Purified proteins were quantified using a BCA protein assay (Pierce).

Labeling:

Article Title: BIAM switch assay coupled to mass spectrometry identifies novel redox targets of NADPH oxidase 4
Article Snippet: .. BIAM labeled proteins were loaded on HisTrap™ FF crude columns packed with Ni-Sepharose (from GE Healthcare) and purified using the Äkta FPLC from GE Healthcare. .. Purified protein was eluted with an 20–500 mM imidazole (from SigmaAldrich) gradient over 20 min with a flow rate of 1 ml/min.

Purification:

Article Title: G-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A
Article Snippet: .. The bacteria were lysed in Buffer A (50 mM sodium dihydrogen phosphate pH 7.5, 0.2 M NaCl, 5 mM Imidazole, 1 × EDTA free protease inhibitor cocktail (Roche 04693132001) and 0.4 mM betamercaptoetanol) and purified on a crude HisTrap column (GE Healthcare 11-0004-58) using Buffer A and Buffer B (50 mM sodium dihydrogen phosphate pH 7.5, 0.2 M NaCl, 500 mM Imidazole, 1 × EDTA free protease inhibitor cocktail (Roche 04693132001) and 0.4 mM betamercaptoetanol). .. Fractions with the highest optical density at 280 nm were pooled, dialyzed to Buffer C (20 mM Tris-HCl, pH 8) and further purified by ion exchange chromatography (Resource Q, GE Healthcare 520348) at pH 8 with buffer C and buffer D (20 mM Tris-HCl pH 8, 1 M NaCl).

Article Title: Screening, large-scale production, and structure-based classification for cystine-dense peptides
Article Snippet: .. CDP fusion proteins were purified by immobilized metal affinity chromatography (IMAC) with HisTrap FF Crude columns (GE) on an ÄKTA Pure FPLC system (GE). .. The CDP was cleaved from the Scn fusion partner by protease digestion, separated from unwanted digestion products by RPC (0.1% w/w TFA in water vs. 0.1% w/w TFA in acetonitrile) on a Tricorn 10/150 column packed with Source 15RPC resin (GE) using an ÄKTA Pure FPLC system, and lyophilized for storage.

Article Title: Purification and Characterization of Native and Vaccine Candidate Mutant Enterotoxigenic Escherichia coli Heat-Stable Toxins
Article Snippet: .. The cleared lysate was purified with Ni-NTA using a HisTrap FF Crude 5 mL column (GE healthcare life sciences, Chicago, IL, USA) connected to an ÄKTA pure system (GE healthcare life sciences). ..

Article Title: Recombinant Envelope-Proteins with Mutations in the Conserved Fusion Loop Allow Specific Serological Diagnosis of Dengue-Infections
Article Snippet: .. The DENV E proteins were purified by immobilized metal affinity chromatography (IMAC) with 5 ml HisTrap FF crude columns (GeHealthcare) and size exclusion chromatography with a 16/600 HiLoad Superdex 200 pg column (GeHealthcare) using the ÄKTA pure 25 l chromatography system (GeHealthcare). .. Purified proteins were quantified using a BCA protein assay (Pierce).

Article Title: BIAM switch assay coupled to mass spectrometry identifies novel redox targets of NADPH oxidase 4
Article Snippet: .. BIAM labeled proteins were loaded on HisTrap™ FF crude columns packed with Ni-Sepharose (from GE Healthcare) and purified using the Äkta FPLC from GE Healthcare. .. Purified protein was eluted with an 20–500 mM imidazole (from SigmaAldrich) gradient over 20 min with a flow rate of 1 ml/min.

Protein Purification:

Article Title: Synthesis of α,β-unsaturated esters via a chemo-enzymatic chain elongation approach by combining carboxylic acid reduction and Wittig reaction
Article Snippet: .. Protein purification was performed on a HisTrapTM FF crude column (GE Healthcare, Piscataway, USA), and the protein was desorbed with an elution buffer (20 mM sodium phosphate, 0.5 M NaCl, 0.5 M imidazole, pH 7.4). .. The purified proteins His-CAR or His-PPTase were dialyzed in a sodium phosphate buffer (50 mM, pH 7.5) and then stored at −20 °C for further use.

Fast Protein Liquid Chromatography:

Article Title: Screening, large-scale production, and structure-based classification for cystine-dense peptides
Article Snippet: .. CDP fusion proteins were purified by immobilized metal affinity chromatography (IMAC) with HisTrap FF Crude columns (GE) on an ÄKTA Pure FPLC system (GE). .. The CDP was cleaved from the Scn fusion partner by protease digestion, separated from unwanted digestion products by RPC (0.1% w/w TFA in water vs. 0.1% w/w TFA in acetonitrile) on a Tricorn 10/150 column packed with Source 15RPC resin (GE) using an ÄKTA Pure FPLC system, and lyophilized for storage.

Article Title: BIAM switch assay coupled to mass spectrometry identifies novel redox targets of NADPH oxidase 4
Article Snippet: .. BIAM labeled proteins were loaded on HisTrap™ FF crude columns packed with Ni-Sepharose (from GE Healthcare) and purified using the Äkta FPLC from GE Healthcare. .. Purified protein was eluted with an 20–500 mM imidazole (from SigmaAldrich) gradient over 20 min with a flow rate of 1 ml/min.

Affinity Purification:

Article Title: A Mutation in CABP2, Expressed in Cochlear Hair Cells, Causes Autosomal-Recessive Hearing Impairment
Article Snippet: .. After centrifugation at 21,280 g, the His6 -tagged proteins were affinity purified from the supernatant with a HisTrap FF crude column (GE Healthcare, Munich, Germany). .. The proteins were eluted from the column, and the His6-tag was cleaved by the incubation of the protein solution with 1 U thrombin per 1 ml protein solution overnight at 4°C.

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    GE Healthcare purification columns histrap crude ff
    Purification Columns Histrap Crude Ff, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/purification columns histrap crude ff/product/GE Healthcare
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    purification columns histrap crude ff - by Bioz Stars, 2020-09
    99/100 stars
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