psti xhoi  (Thermo Fisher)


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    Structured Review

    Thermo Fisher psti xhoi
    Psti Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psti xhoi/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psti xhoi - by Bioz Stars, 2020-03
    91/100 stars

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    Related Articles

    Countercurrent Chromatography:

    Article Title: Inducible, Dose-Adjustable and Time-Restricted Reconstitution of Stat1 Deficiency In Vivo
    Article Snippet: Stat1 -fwd: 5′ GAT CAG CTG CAA ACG TGG TTC 3′; Stat1 -rev: 5′ GCT TTT TAA GCT GCT GAC GGA 3′; Stat1 -fam: 5′CCA TT G TTG CAG AGA CCC TGC AGC A 3′; Mx1 -fwd: 5′ CCT GGA GGA GCA GAG TGA CAC 3′; Mx1 -rev: 5′ GG T TAA TCG GAG AAT TTG GCA A 3′; Mx1 -fam: 5′ TT A AGG CTG GAT GAG GCT CGG CAG A 3′; Irf1 -fwd: 5′ CCG AAG ACC TTA TGA AGC TCT TTG 3′; Irf1 -rev: 5′ GCA AGT ATC CCT TGC CAT CG 3′; Irf1 -fam: 5′ CA G TCT GAG TGG CAG CCG ACA CAC A 3′. .. Southern blot analysis was performed using a 5′-external Col1a1 probe of 400 bp isolated with PstI/XhoI (Thermo Scientific) digestion .

    Clone Assay:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: .. Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. The GFP expression cassette was removed from this plasmid by HindIII–BamHI digestion and self-circularized to obtain pdelCpG.

    Article Title: Inducible, Dose-Adjustable and Time-Restricted Reconstitution of Stat1 Deficiency In Vivo
    Article Snippet: Southern blot analysis was performed using a 5′-external Col1a1 probe of 400 bp isolated with PstI/XhoI (Thermo Scientific) digestion . .. Genomic DNA of gene-targeted clones was digested with SpeI (Thermo Scientific).

    Luciferase:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. Fragment containing the integrase was excised from pVAX1-Int by PmeI digestion and cloned into PmeI site of pdelCpG to generate pdelCpG-Int. mRNA coding for the φC31 integrase was produced by CureVac (CUREVAC GmbH, Tuebingen, Germany). pVAX1-Luc was constructed as a shuttle vector by excising firefly luciferase cDNA from pGL3-Basic vector (Promega, Madison, WI, USA) by HindIII–XbaI digestion and cloning into the corresponding sites of pVAX1.

    Southern Blot:

    Article Title: Inducible, Dose-Adjustable and Time-Restricted Reconstitution of Stat1 Deficiency In Vivo
    Article Snippet: .. Southern blot analysis was performed using a 5′-external Col1a1 probe of 400 bp isolated with PstI/XhoI (Thermo Scientific) digestion . .. Genomic DNA of gene-targeted clones was digested with SpeI (Thermo Scientific).

    Isolation:

    Article Title: Inducible, Dose-Adjustable and Time-Restricted Reconstitution of Stat1 Deficiency In Vivo
    Article Snippet: .. Southern blot analysis was performed using a 5′-external Col1a1 probe of 400 bp isolated with PstI/XhoI (Thermo Scientific) digestion . .. Genomic DNA of gene-targeted clones was digested with SpeI (Thermo Scientific).

    Sequencing:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: .. Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. The GFP expression cassette was removed from this plasmid by HindIII–BamHI digestion and self-circularized to obtain pdelCpG.

    Construct:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: .. Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. The GFP expression cassette was removed from this plasmid by HindIII–BamHI digestion and self-circularized to obtain pdelCpG.

    Produced:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. Fragment containing the integrase was excised from pVAX1-Int by PmeI digestion and cloned into PmeI site of pdelCpG to generate pdelCpG-Int. mRNA coding for the φC31 integrase was produced by CureVac (CUREVAC GmbH, Tuebingen, Germany). pVAX1-Luc was constructed as a shuttle vector by excising firefly luciferase cDNA from pGL3-Basic vector (Promega, Madison, WI, USA) by HindIII–XbaI digestion and cloning into the corresponding sites of pVAX1.

    CTG Assay:

    Article Title: Inducible, Dose-Adjustable and Time-Restricted Reconstitution of Stat1 Deficiency In Vivo
    Article Snippet: Stat1 -fwd: 5′ GAT CAG CTG CAA ACG TGG TTC 3′; Stat1 -rev: 5′ GCT TTT TAA GCT GCT GAC GGA 3′; Stat1 -fam: 5′CCA TT G TTG CAG AGA CCC TGC AGC A 3′; Mx1 -fwd: 5′ CCT GGA GGA GCA GAG TGA CAC 3′; Mx1 -rev: 5′ GG T TAA TCG GAG AAT TTG GCA A 3′; Mx1 -fam: 5′ TT A AGG CTG GAT GAG GCT CGG CAG A 3′; Irf1 -fwd: 5′ CCG AAG ACC TTA TGA AGC TCT TTG 3′; Irf1 -rev: 5′ GCA AGT ATC CCT TGC CAT CG 3′; Irf1 -fam: 5′ CA G TCT GAG TGG CAG CCG ACA CAC A 3′. .. Southern blot analysis was performed using a 5′-external Col1a1 probe of 400 bp isolated with PstI/XhoI (Thermo Scientific) digestion .

    Amplification:

    Article Title: Inducible, Dose-Adjustable and Time-Restricted Reconstitution of Stat1 Deficiency In Vivo
    Article Snippet: Stat1 : Stat1 -fwd: 5′ TG T TCT TCC TCT CCC TTT AGG A 3′; Stat1 -rev: 5′ CAC ATG GAC TGC TGT GCT TT 3′; Stat1 -mut: 5′ GGG TGG GGT GGG ATT AGA TA 3 (amplicon size for WT: 335 bp; Stat1ind : 372 bp). .. Southern blot analysis was performed using a 5′-external Col1a1 probe of 400 bp isolated with PstI/XhoI (Thermo Scientific) digestion .

    Blocking Assay:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: .. Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. The GFP expression cassette was removed from this plasmid by HindIII–BamHI digestion and self-circularized to obtain pdelCpG.

    Expressing:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: .. Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. The GFP expression cassette was removed from this plasmid by HindIII–BamHI digestion and self-circularized to obtain pdelCpG.

    Polymerase Chain Reaction:

    Article Title: Inducible, Dose-Adjustable and Time-Restricted Reconstitution of Stat1 Deficiency In Vivo
    Article Snippet: Paragraph title: PCR, RT-qPCR and Southern Blot ... Southern blot analysis was performed using a 5′-external Col1a1 probe of 400 bp isolated with PstI/XhoI (Thermo Scientific) digestion .

    Quantitative RT-PCR:

    Article Title: Inducible, Dose-Adjustable and Time-Restricted Reconstitution of Stat1 Deficiency In Vivo
    Article Snippet: Paragraph title: PCR, RT-qPCR and Southern Blot ... Southern blot analysis was performed using a 5′-external Col1a1 probe of 400 bp isolated with PstI/XhoI (Thermo Scientific) digestion .

    Cellular Antioxidant Activity Assay:

    Article Title: Inducible, Dose-Adjustable and Time-Restricted Reconstitution of Stat1 Deficiency In Vivo
    Article Snippet: Stat1 -fwd: 5′ GAT CAG CTG CAA ACG TGG TTC 3′; Stat1 -rev: 5′ GCT TTT TAA GCT GCT GAC GGA 3′; Stat1 -fam: 5′CCA TT G TTG CAG AGA CCC TGC AGC A 3′; Mx1 -fwd: 5′ CCT GGA GGA GCA GAG TGA CAC 3′; Mx1 -rev: 5′ GG T TAA TCG GAG AAT TTG GCA A 3′; Mx1 -fam: 5′ TT A AGG CTG GAT GAG GCT CGG CAG A 3′; Irf1 -fwd: 5′ CCG AAG ACC TTA TGA AGC TCT TTG 3′; Irf1 -rev: 5′ GCA AGT ATC CCT TGC CAT CG 3′; Irf1 -fam: 5′ CA G TCT GAG TGG CAG CCG ACA CAC A 3′. .. Southern blot analysis was performed using a 5′-external Col1a1 probe of 400 bp isolated with PstI/XhoI (Thermo Scientific) digestion .

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Inducible, Dose-Adjustable and Time-Restricted Reconstitution of Stat1 Deficiency In Vivo
    Article Snippet: Stat1 -fwd: 5′ GAT CAG CTG CAA ACG TGG TTC 3′; Stat1 -rev: 5′ GCT TTT TAA GCT GCT GAC GGA 3′; Stat1 -fam: 5′CCA TT G TTG CAG AGA CCC TGC AGC A 3′; Mx1 -fwd: 5′ CCT GGA GGA GCA GAG TGA CAC 3′; Mx1 -rev: 5′ GG T TAA TCG GAG AAT TTG GCA A 3′; Mx1 -fam: 5′ TT A AGG CTG GAT GAG GCT CGG CAG A 3′; Irf1 -fwd: 5′ CCG AAG ACC TTA TGA AGC TCT TTG 3′; Irf1 -rev: 5′ GCA AGT ATC CCT TGC CAT CG 3′; Irf1 -fam: 5′ CA G TCT GAG TGG CAG CCG ACA CAC A 3′. .. Southern blot analysis was performed using a 5′-external Col1a1 probe of 400 bp isolated with PstI/XhoI (Thermo Scientific) digestion .

    Plasmid Preparation:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. The GFP expression cassette was removed from this plasmid by HindIII–BamHI digestion and self-circularized to obtain pdelCpG.

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  • 91
    Thermo Fisher psti xhoi
    Psti Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psti xhoi/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psti xhoi - by Bioz Stars, 2020-03
    91/100 stars
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