psti xhoi  (Thermo Fisher)


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    Structured Review

    Thermo Fisher psti xhoi
    Psti Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psti xhoi/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psti xhoi - by Bioz Stars, 2020-09
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: .. Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. The GFP expression cassette was removed from this plasmid by HindIII–BamHI digestion and self-circularized to obtain pdelCpG.

    Southern Blot:

    Article Title: Inducible, Dose-Adjustable and Time-Restricted Reconstitution of Stat1 Deficiency In Vivo
    Article Snippet: .. Southern blot analysis was performed using a 5′-external Col1a1 probe of 400 bp isolated with PstI/XhoI (Thermo Scientific) digestion . .. Genomic DNA of gene-targeted clones was digested with SpeI (Thermo Scientific).

    Isolation:

    Article Title: Inducible, Dose-Adjustable and Time-Restricted Reconstitution of Stat1 Deficiency In Vivo
    Article Snippet: .. Southern blot analysis was performed using a 5′-external Col1a1 probe of 400 bp isolated with PstI/XhoI (Thermo Scientific) digestion . .. Genomic DNA of gene-targeted clones was digested with SpeI (Thermo Scientific).

    Blocking Assay:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: .. Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. The GFP expression cassette was removed from this plasmid by HindIII–BamHI digestion and self-circularized to obtain pdelCpG.

    Construct:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: .. Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. The GFP expression cassette was removed from this plasmid by HindIII–BamHI digestion and self-circularized to obtain pdelCpG.

    Expressing:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: .. Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. The GFP expression cassette was removed from this plasmid by HindIII–BamHI digestion and self-circularized to obtain pdelCpG.

    Sequencing:

    Article Title: Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
    Article Snippet: .. Plasmids pCMVInt has been previously described ( , , ) and was kindly provided by M. P. Calos (Stanford University, USA). pCAG-Int(NLS) and pPGK-Int(NLS) coding for an integrase fused with a C-terminal nuclear localization signal (NLS) of SV40 T antigen, driven by CAG and PGK promoters, respectively, and pSVpaxattP 50-attB 53, comprising an ‘expression blocking’ sequence flanked by attB and attP sites between a P1 promoter and a lacZ gene, were kindly provided by R. Kuehn (Helmholtz Zentrum Munich, German Research Centre for Environmental Health, Germany). pC31-Intopt was obtained from Addgene Inc. (Cambridge, MA, USA). pVAX1-Int was constructed by excising the integrase cDNA from pCMV-Int by PstI-XhoI (Fermentas, St. Leon-Rot, Germany) digestion and cloning into respective sites of pVAX1 (Invitrogen, Paisley, UK). pdelCpG-GFP was obtained from Geneart (GENEART AG, Regensburg, Germany) and contains the same expression cassette as pVAX1 but has reduced number of CpG motifs. .. The GFP expression cassette was removed from this plasmid by HindIII–BamHI digestion and self-circularized to obtain pdelCpG.

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  • 86
    Thermo Fisher psti xhoi
    Psti Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psti xhoi/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psti xhoi - by Bioz Stars, 2020-09
    86/100 stars
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