psti fastdigest  (Thermo Fisher)


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    Name:
    FastDigest PstI
    Description:
    5 C T G C A ↓G 3 3 G ↑A C G T C 5 Thermo Scientific FastDigest PstI restriction enzyme recognizes CTGCA G site and cuts best at 37°C in 5 15 minutes using universal FastDigest Buffer Isoschizomers BspMAI Thermo Scientific FastDigest PstI is one of an advanced line of fast restriction enzymes that are all 100 active in the universal FastDigest and FastDigest Green reaction buffers The universal buffer allows rapid single double or multiple DNA digestion within 5 15 minutes eliminating any need for buffer change or subsequent DNA clean up steps See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity heat inactivation and incubation times for this and other FastDigest restriction enzymes DNA modifying enzymes such as Klenow Fragment T4 DNA Ligase alkaline phosphatases and T4 DNA Polymerase all have 100 activity in FastDigest Buffer Therefore enzymes for downstream applications can be directly added to the FastDigest reaction mix For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects Features• 100 activity of all FastDigest enzymes in the universal buffer• 100 buffer compatibility with downstream applications• Complete digestion in 5 15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes availableApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern Blot• Restriction fragment length polymorphism RFLP • SNP analysisNote The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer For applications that require product analysis by fluorescence excitation e g concentration measurements in UV light the colorless FastDigest Buffer is recommended For methylation sensitivity refer to product specifications
    Catalog Number:
    fd0614
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher psti fastdigest
    5 C T G C A ↓G 3 3 G ↑A C G T C 5 Thermo Scientific FastDigest PstI restriction enzyme recognizes CTGCA G site and cuts best at 37°C in 5 15 minutes using universal FastDigest Buffer Isoschizomers BspMAI Thermo Scientific FastDigest PstI is one of an advanced line of fast restriction enzymes that are all 100 active in the universal FastDigest and FastDigest Green reaction buffers The universal buffer allows rapid single double or multiple DNA digestion within 5 15 minutes eliminating any need for buffer change or subsequent DNA clean up steps See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity heat inactivation and incubation times for this and other FastDigest restriction enzymes DNA modifying enzymes such as Klenow Fragment T4 DNA Ligase alkaline phosphatases and T4 DNA Polymerase all have 100 activity in FastDigest Buffer Therefore enzymes for downstream applications can be directly added to the FastDigest reaction mix For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects Features• 100 activity of all FastDigest enzymes in the universal buffer• 100 buffer compatibility with downstream applications• Complete digestion in 5 15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes availableApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern Blot• Restriction fragment length polymorphism RFLP • SNP analysisNote The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer For applications that require product analysis by fluorescence excitation e g concentration measurements in UV light the colorless FastDigest Buffer is recommended For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/psti fastdigest/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psti fastdigest - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Genome-wide, time-sensitive interrogation of the heat shock response under diverse stressors via ReporterSeq
    Article Snippet: .. Plasmid library sequencing Two µg of plasmid was cut with the FastDigest PstI restriction enzyme (Thermo Fisher) and the 900 bp piece was gel purified using a gel DNA extraction kit (Zymo). .. This piece was then recirculized in a 20 µL reaction containing 60 ng of gel purified DNA and 1 µL T4 DNA Ligase (Thermo Fisher) in buffer.

    Sequencing:

    Article Title: Genome-wide, time-sensitive interrogation of the heat shock response under diverse stressors via ReporterSeq
    Article Snippet: .. Plasmid library sequencing Two µg of plasmid was cut with the FastDigest PstI restriction enzyme (Thermo Fisher) and the 900 bp piece was gel purified using a gel DNA extraction kit (Zymo). .. This piece was then recirculized in a 20 µL reaction containing 60 ng of gel purified DNA and 1 µL T4 DNA Ligase (Thermo Fisher) in buffer.

    Agarose Gel Electrophoresis:

    Article Title: High Diversity of Extended-Spectrum ?-Lactamases in Escherichia coli Isolates from Italian Broiler Flocks ▿
    Article Snippet: .. Twelve different plasmid profiles were visualized on a 0.8% agarose gel after enzymatic digestion with ClaI and PstI (FastDigest; Fermentas, Helsingborg, Sweden). ..

    Concentration Assay:

    Article Title: Evolution of neuronal anatomy and circuitry in two highly divergent nematode species
    Article Snippet: .. To create complex arrays for transgenesis, wildtype PS312 genomic DNA, the PCR product or the plasmid carrying the target reporter, and the Ppa-egl-20::rfp reporter (co-injection marker expressed in the tail) were digested with the FastDigest PstI restriction enzyme (Thermo Fisher Scientific, FD0615) and then mixed at the final concentration of 60 ng/µl for genomic DNA and 10 ng/µl for each plasmid. .. Prepared mix was injected in the gonad rachis in hermaphrodites ( ; ).

    Purification:

    Article Title: Genome-wide, time-sensitive interrogation of the heat shock response under diverse stressors via ReporterSeq
    Article Snippet: .. Plasmid library sequencing Two µg of plasmid was cut with the FastDigest PstI restriction enzyme (Thermo Fisher) and the 900 bp piece was gel purified using a gel DNA extraction kit (Zymo). .. This piece was then recirculized in a 20 µL reaction containing 60 ng of gel purified DNA and 1 µL T4 DNA Ligase (Thermo Fisher) in buffer.

    Article Title: Metformin Attenuates Palmitate-Induced Endoplasmic Reticulum Stress, Serine Phosphorylation of IRS-1 and Apoptosis in Rat Insulinoma Cells
    Article Snippet: .. Briefly, 200 ng purified PCR product was digested with FastDigest PstI (Thermo Scientific) for 30 min at 37°C. .. The amplified sequence of unspliced XBP-1 is cut in two fragments (153 and 294 bp) by PstI while the spliced variant remains uncut .

    Article Title: Effect of cis- and trans-Monounsaturated Fatty Acids on Palmitate Toxicity and on Palmitate-induced Accumulation of Ceramides and Diglycerides
    Article Snippet: .. For a better visibility, PstI restriction endonuclease cleavage of 200 ng purified PCR product was carried out by using FastDigest PstI (Thermo Fisher Scientific; Waltham, MA, USA) for 30 min at 37 °C. .. The unspliced XBP-1 is cut into two fragments (153 and 294 bp) by PstI while the spliced variant remains uncut [ ].

    Polymerase Chain Reaction:

    Article Title: Metformin Attenuates Palmitate-Induced Endoplasmic Reticulum Stress, Serine Phosphorylation of IRS-1 and Apoptosis in Rat Insulinoma Cells
    Article Snippet: .. Briefly, 200 ng purified PCR product was digested with FastDigest PstI (Thermo Scientific) for 30 min at 37°C. .. The amplified sequence of unspliced XBP-1 is cut in two fragments (153 and 294 bp) by PstI while the spliced variant remains uncut .

    Article Title: Heterologous Biosynthesis, Modifications and Structural Characterization of Ruminococcin-A, a Lanthipeptide From the Gut Bacterium Ruminococcus gnavus E1, in Escherichia coli
    Article Snippet: .. The resulting rumA1 PCR product was digested with NheI and PstI FastDigest enzymes (Thermo Scientific, Darmstadt, Germany) and ligated into NheI / PstI -digested pCTUT7 vector yielding pLEO rA . .. For the expression of ruminococcin-A lanthionine synthetase RumM, PCRs with primer pairs rumM_f2/rumM_r and rumM_f3/rumM_r using pLEO rC2 as template were performed, and the resulting amplicons were digested with NheI / PstI and BamHI / PstI , and further ligated into the pCTUT7 and pJL10 expression vectors generating pLEO rM ’ and pLEO rM , respectively.

    Article Title: Effect of cis- and trans-Monounsaturated Fatty Acids on Palmitate Toxicity and on Palmitate-induced Accumulation of Ceramides and Diglycerides
    Article Snippet: .. For a better visibility, PstI restriction endonuclease cleavage of 200 ng purified PCR product was carried out by using FastDigest PstI (Thermo Fisher Scientific; Waltham, MA, USA) for 30 min at 37 °C. .. The unspliced XBP-1 is cut into two fragments (153 and 294 bp) by PstI while the spliced variant remains uncut [ ].

    Article Title: Heterologous Biosynthesis, Modifications and Structural Characterization of Ruminococcin-A, a Lanthipeptide From the Gut Bacterium Ruminococcus gnavus E1, in Escherichia coli
    Article Snippet: .. The resulting rumA1 PCR product was digested with NheI and PstI FastDigest enzymes (Thermo Scientific, Darmstadt, Germany) and ligated into NheI /PstI -digested pCTUT7 vector yielding pLEOrA . .. For the expression of ruminococcin-A lanthionine synthetase RumM, PCRs with primer pairs rumM_f2/rumM_r and rumM_f3/rumM_r using pLEOrC2 as template were performed, and the resulting amplicons were digested with NheI /PstI and BamHI /PstI , and further ligated into the pCTUT7 and pJL10 expression vectors generating pLEOrM ’ and pLEOrM , respectively.

    Article Title: Evolution of neuronal anatomy and circuitry in two highly divergent nematode species
    Article Snippet: .. To create complex arrays for transgenesis, wildtype PS312 genomic DNA, the PCR product or the plasmid carrying the target reporter, and the Ppa-egl-20::rfp reporter (co-injection marker expressed in the tail) were digested with the FastDigest PstI restriction enzyme (Thermo Fisher Scientific, FD0615) and then mixed at the final concentration of 60 ng/µl for genomic DNA and 10 ng/µl for each plasmid. .. Prepared mix was injected in the gonad rachis in hermaphrodites ( ; ).

    Marker:

    Article Title: Evolution of neuronal anatomy and circuitry in two highly divergent nematode species
    Article Snippet: .. To create complex arrays for transgenesis, wildtype PS312 genomic DNA, the PCR product or the plasmid carrying the target reporter, and the Ppa-egl-20::rfp reporter (co-injection marker expressed in the tail) were digested with the FastDigest PstI restriction enzyme (Thermo Fisher Scientific, FD0615) and then mixed at the final concentration of 60 ng/µl for genomic DNA and 10 ng/µl for each plasmid. .. Prepared mix was injected in the gonad rachis in hermaphrodites ( ; ).

    Plasmid Preparation:

    Article Title: Genome-wide, time-sensitive interrogation of the heat shock response under diverse stressors via ReporterSeq
    Article Snippet: .. Plasmid library sequencing Two µg of plasmid was cut with the FastDigest PstI restriction enzyme (Thermo Fisher) and the 900 bp piece was gel purified using a gel DNA extraction kit (Zymo). .. This piece was then recirculized in a 20 µL reaction containing 60 ng of gel purified DNA and 1 µL T4 DNA Ligase (Thermo Fisher) in buffer.

    Article Title: Heterologous Biosynthesis, Modifications and Structural Characterization of Ruminococcin-A, a Lanthipeptide From the Gut Bacterium Ruminococcus gnavus E1, in Escherichia coli
    Article Snippet: .. The resulting rumA1 PCR product was digested with NheI and PstI FastDigest enzymes (Thermo Scientific, Darmstadt, Germany) and ligated into NheI / PstI -digested pCTUT7 vector yielding pLEO rA . .. For the expression of ruminococcin-A lanthionine synthetase RumM, PCRs with primer pairs rumM_f2/rumM_r and rumM_f3/rumM_r using pLEO rC2 as template were performed, and the resulting amplicons were digested with NheI / PstI and BamHI / PstI , and further ligated into the pCTUT7 and pJL10 expression vectors generating pLEO rM ’ and pLEO rM , respectively.

    Article Title: Heterologous Biosynthesis, Modifications and Structural Characterization of Ruminococcin-A, a Lanthipeptide From the Gut Bacterium Ruminococcus gnavus E1, in Escherichia coli
    Article Snippet: .. The resulting rumA1 PCR product was digested with NheI and PstI FastDigest enzymes (Thermo Scientific, Darmstadt, Germany) and ligated into NheI /PstI -digested pCTUT7 vector yielding pLEOrA . .. For the expression of ruminococcin-A lanthionine synthetase RumM, PCRs with primer pairs rumM_f2/rumM_r and rumM_f3/rumM_r using pLEOrC2 as template were performed, and the resulting amplicons were digested with NheI /PstI and BamHI /PstI , and further ligated into the pCTUT7 and pJL10 expression vectors generating pLEOrM ’ and pLEOrM , respectively.

    Article Title: High Diversity of Extended-Spectrum ?-Lactamases in Escherichia coli Isolates from Italian Broiler Flocks ▿
    Article Snippet: .. Twelve different plasmid profiles were visualized on a 0.8% agarose gel after enzymatic digestion with ClaI and PstI (FastDigest; Fermentas, Helsingborg, Sweden). ..

    Article Title: Evolution of neuronal anatomy and circuitry in two highly divergent nematode species
    Article Snippet: .. To create complex arrays for transgenesis, wildtype PS312 genomic DNA, the PCR product or the plasmid carrying the target reporter, and the Ppa-egl-20::rfp reporter (co-injection marker expressed in the tail) were digested with the FastDigest PstI restriction enzyme (Thermo Fisher Scientific, FD0615) and then mixed at the final concentration of 60 ng/µl for genomic DNA and 10 ng/µl for each plasmid. .. Prepared mix was injected in the gonad rachis in hermaphrodites ( ; ).

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  • 95
    Thermo Fisher fastdigest psti
    Fastdigest Psti, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest psti/product/Thermo Fisher
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fastdigest psti - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    94
    Thermo Fisher fastdigest psti restriction enzyme
    Fastdigest Psti Restriction Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest psti restriction enzyme/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fastdigest psti restriction enzyme - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    98
    Thermo Fisher psti restriction enzymes
    Psti Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psti restriction enzymes/product/Thermo Fisher
    Average 98 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    psti restriction enzymes - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

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