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psre l luciferase reporter plasmid (Agilent technologies)

Name: psre l luciferase reporter plasmid
Brand: Agilent technologies
Rating: 86 (1 reviews)

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Agilent technologies psre l luciferase reporter plasmid

FHL1A enhanced PLEKHG2-induced SRE-dependent gene transcription. A, HEK293 cells were co-transfected <t>with</t> <t>pSRE.L-luciferase,</t> pRL-SV40, PLEKHG2, PLEKHG2 P2, FHL1A, and FHL1B, as indicated. The cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. B, HEK293 cells were co-transfected with PLEKHG2, PLEKHG2 P2, FHL1A, and FHL1B, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FLAG antibody (for FHL1). PLEK2, PLEKHG2. IB, immunoblotting.

Psre L Luciferase Reporter Plasmid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Four-and-a-half LIM Domains 1 (FHL1) Protein Interacts with the Rho Guanine Nucleotide Exchange Factor PLEKHG2/FLJ00018 and Regulates Cell Morphogenesis"

Article Title: Four-and-a-half LIM Domains 1 (FHL1) Protein Interacts with the Rho Guanine Nucleotide Exchange Factor PLEKHG2/FLJ00018 and Regulates Cell Morphogenesis

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M116.759571

FHL1A enhanced PLEKHG2-induced SRE-dependent gene transcription. A, HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, PLEKHG2 P2, FHL1A, and FHL1B, as indicated. The cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. B, HEK293 cells were co-transfected with PLEKHG2, PLEKHG2 P2, FHL1A, and FHL1B, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FLAG antibody (for FHL1). PLEK2, PLEKHG2. IB, immunoblotting.

Figure Legend Snippet: FHL1A enhanced PLEKHG2-induced SRE-dependent gene transcription. A, HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, PLEKHG2 P2, FHL1A, and FHL1B, as indicated. The cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. B, HEK293 cells were co-transfected with PLEKHG2, PLEKHG2 P2, FHL1A, and FHL1B, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FLAG antibody (for FHL1). PLEK2, PLEKHG2. IB, immunoblotting.

Techniques Used: Transfection, Luciferase, Reporter Gene Assay, SDS Page, Western Blot

Gβγ enhanced FHL1A- and PLEKHG2-induced SRE-dependent gene transcription. A and D, HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, PLEKHG2 P2, FHL1A, FHL1B, and Gβγ, as indicated. The cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. B, C, E, and F, HEK293 cells were co-transfected with PLEKHG2, PLEKHG2 P2, FHL1A, FHL1B, and Gβγ, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FLAG antibody (for FHL1). PLEK2, PLEKHG2. IB, immunoblotting.

Figure Legend Snippet: Gβγ enhanced FHL1A- and PLEKHG2-induced SRE-dependent gene transcription. A and D, HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, PLEKHG2 P2, FHL1A, FHL1B, and Gβγ, as indicated. The cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. B, C, E, and F, HEK293 cells were co-transfected with PLEKHG2, PLEKHG2 P2, FHL1A, FHL1B, and Gβγ, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FLAG antibody (for FHL1). PLEK2, PLEKHG2. IB, immunoblotting.

Techniques Used: Transfection, Luciferase, Reporter Gene Assay, SDS Page, Western Blot

β-Actin inhibited the FHL1A-activated, PLEKHG2-induced SRE-dependent gene transcription. A, HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, FHL1A, and β-actin, as indicated. Cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars) The data shown are representative of three independent experiments. B, HEK293 cells were co-transfected with PLEKHG2, FHL1A, and β-actin, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) or anti-FLAG antibody (for FHL1 and β-actin). PLEK2, PLEKHG2. IB, immunoblotting.

Figure Legend Snippet: β-Actin inhibited the FHL1A-activated, PLEKHG2-induced SRE-dependent gene transcription. A, HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, FHL1A, and β-actin, as indicated. Cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars) The data shown are representative of three independent experiments. B, HEK293 cells were co-transfected with PLEKHG2, FHL1A, and β-actin, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) or anti-FLAG antibody (for FHL1 and β-actin). PLEK2, PLEKHG2. IB, immunoblotting.

Techniques Used: Transfection, Luciferase, Reporter Gene Assay, SDS Page, Western Blot

Effect of FHL1 on Gβγ- and PLEKHG2-induced SRE-dependent gene transcription. A, wild type or FHL1-knock-out HEK293 cells were transfected with Myc-tagged PLEKHG2, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FHL1 antibody (for FHL1). PLEK2, PLEKHG2. B and C, wild type or FHL1-knock-out HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, Gβγ, and FHL1A, as indicated. Cells were lysed 24 h after transfection, and the SRE-dependent gene transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. *, p < 0.05 compared with PLEKHG2- and Gβγ-expressing HEK293 WT cells. n.s., not significant. D, wild type or FHL1-knock-out HEK293 cells were co-transfected with PLEKHG2, Gβγ, and FHL1A, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2), anti-FLAG antibody (for FHL1), Gβ antibody (for Gβ), or anti-tubulin-γ antibody (for γ-tubulin). PLEK2, PLEKHG2. IB, immunoblotting.

Figure Legend Snippet: Effect of FHL1 on Gβγ- and PLEKHG2-induced SRE-dependent gene transcription. A, wild type or FHL1-knock-out HEK293 cells were transfected with Myc-tagged PLEKHG2, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FHL1 antibody (for FHL1). PLEK2, PLEKHG2. B and C, wild type or FHL1-knock-out HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, Gβγ, and FHL1A, as indicated. Cells were lysed 24 h after transfection, and the SRE-dependent gene transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. *, p < 0.05 compared with PLEKHG2- and Gβγ-expressing HEK293 WT cells. n.s., not significant. D, wild type or FHL1-knock-out HEK293 cells were co-transfected with PLEKHG2, Gβγ, and FHL1A, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2), anti-FLAG antibody (for FHL1), Gβ antibody (for Gβ), or anti-tubulin-γ antibody (for γ-tubulin). PLEK2, PLEKHG2. IB, immunoblotting.

Techniques Used: Knock-Out, Transfection, SDS Page, Luciferase, Reporter Gene Assay, Expressing, Western Blot

PLEKHG2 and FLH1A were co-localized in Neuro-2a cells and regulated cell morphogenesis. A and D, Neuro-2a cells were co-transfected with Myc-tagged PLEKHG2 and FLAG-tagged FHL1A or FHL1B, as indicated. Cells were lysed 24 h after transfection, and PLEKHG2 was immunoprecipitated with anti-Myc antibody. Precipitated proteins were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) or anti-FLAG antibody (for FHL1). B and E, Neuro-2a cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, FHL1A, FHL1B, and Gβγ, as indicated. Cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. C and F, Neuro-2a cells were co-transfected with PLEKHG2, FHL1A, FHL1B, and Gβγ, as indicated. Cells were lysed 24 h after transfection, and the lysate was separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FLAG antibody (for FHL1). PLEK2, PLEKHG2; TCL, total cell lysate; IP, immunoprecipitation. IB, immunoblotting. *, immunoglobulins precipitated from immunoprecipitation.

Figure Legend Snippet: PLEKHG2 and FLH1A were co-localized in Neuro-2a cells and regulated cell morphogenesis. A and D, Neuro-2a cells were co-transfected with Myc-tagged PLEKHG2 and FLAG-tagged FHL1A or FHL1B, as indicated. Cells were lysed 24 h after transfection, and PLEKHG2 was immunoprecipitated with anti-Myc antibody. Precipitated proteins were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) or anti-FLAG antibody (for FHL1). B and E, Neuro-2a cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, FHL1A, FHL1B, and Gβγ, as indicated. Cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. C and F, Neuro-2a cells were co-transfected with PLEKHG2, FHL1A, FHL1B, and Gβγ, as indicated. Cells were lysed 24 h after transfection, and the lysate was separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FLAG antibody (for FHL1). PLEK2, PLEKHG2; TCL, total cell lysate; IP, immunoprecipitation. IB, immunoblotting. *, immunoglobulins precipitated from immunoprecipitation.

Techniques Used: Transfection, Immunoprecipitation, SDS Page, Luciferase, Reporter Gene Assay, Western Blot

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Journal: The Journal of Biological Chemistry

Article Title: Four-and-a-half LIM Domains 1 (FHL1) Protein Interacts with the Rho Guanine Nucleotide Exchange Factor PLEKHG2/FLJ00018 and Regulates Cell Morphogenesis

doi: 10.1074/jbc.M116.759571

Figure Lengend Snippet: FHL1A enhanced PLEKHG2-induced SRE-dependent gene transcription. A, HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, PLEKHG2 P2, FHL1A, and FHL1B, as indicated. The cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. B, HEK293 cells were co-transfected with PLEKHG2, PLEKHG2 P2, FHL1A, and FHL1B, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FLAG antibody (for FHL1). PLEK2, PLEKHG2. IB, immunoblotting.

Article Snippet: The pSRE.L-luciferase reporter plasmid was purchased from Stratagene (Santa Clara, CA), and pRL-SV40 was purchased from Nippon Gene (Tokyo, Japan).

Techniques: Transfection, Luciferase, Reporter Gene Assay, SDS Page, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: Four-and-a-half LIM Domains 1 (FHL1) Protein Interacts with the Rho Guanine Nucleotide Exchange Factor PLEKHG2/FLJ00018 and Regulates Cell Morphogenesis

doi: 10.1074/jbc.M116.759571

Figure Lengend Snippet: Gβγ enhanced FHL1A- and PLEKHG2-induced SRE-dependent gene transcription. A and D, HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, PLEKHG2 P2, FHL1A, FHL1B, and Gβγ, as indicated. The cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. B, C, E, and F, HEK293 cells were co-transfected with PLEKHG2, PLEKHG2 P2, FHL1A, FHL1B, and Gβγ, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FLAG antibody (for FHL1). PLEK2, PLEKHG2. IB, immunoblotting.

Article Snippet: The pSRE.L-luciferase reporter plasmid was purchased from Stratagene (Santa Clara, CA), and pRL-SV40 was purchased from Nippon Gene (Tokyo, Japan).

Techniques: Transfection, Luciferase, Reporter Gene Assay, SDS Page, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: Four-and-a-half LIM Domains 1 (FHL1) Protein Interacts with the Rho Guanine Nucleotide Exchange Factor PLEKHG2/FLJ00018 and Regulates Cell Morphogenesis

doi: 10.1074/jbc.M116.759571

Figure Lengend Snippet: β-Actin inhibited the FHL1A-activated, PLEKHG2-induced SRE-dependent gene transcription. A, HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, FHL1A, and β-actin, as indicated. Cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars) The data shown are representative of three independent experiments. B, HEK293 cells were co-transfected with PLEKHG2, FHL1A, and β-actin, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) or anti-FLAG antibody (for FHL1 and β-actin). PLEK2, PLEKHG2. IB, immunoblotting.

Article Snippet: The pSRE.L-luciferase reporter plasmid was purchased from Stratagene (Santa Clara, CA), and pRL-SV40 was purchased from Nippon Gene (Tokyo, Japan).

Techniques: Transfection, Luciferase, Reporter Gene Assay, SDS Page, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: Four-and-a-half LIM Domains 1 (FHL1) Protein Interacts with the Rho Guanine Nucleotide Exchange Factor PLEKHG2/FLJ00018 and Regulates Cell Morphogenesis

doi: 10.1074/jbc.M116.759571

Figure Lengend Snippet: Effect of FHL1 on Gβγ- and PLEKHG2-induced SRE-dependent gene transcription. A, wild type or FHL1-knock-out HEK293 cells were transfected with Myc-tagged PLEKHG2, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FHL1 antibody (for FHL1). PLEK2, PLEKHG2. B and C, wild type or FHL1-knock-out HEK293 cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, Gβγ, and FHL1A, as indicated. Cells were lysed 24 h after transfection, and the SRE-dependent gene transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. *, p < 0.05 compared with PLEKHG2- and Gβγ-expressing HEK293 WT cells. n.s., not significant. D, wild type or FHL1-knock-out HEK293 cells were co-transfected with PLEKHG2, Gβγ, and FHL1A, as indicated. Cells were lysed 24 h after transfection, and the lysates were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2), anti-FLAG antibody (for FHL1), Gβ antibody (for Gβ), or anti-tubulin-γ antibody (for γ-tubulin). PLEK2, PLEKHG2. IB, immunoblotting.

Article Snippet: The pSRE.L-luciferase reporter plasmid was purchased from Stratagene (Santa Clara, CA), and pRL-SV40 was purchased from Nippon Gene (Tokyo, Japan).

Techniques: Knock-Out, Transfection, SDS Page, Luciferase, Reporter Gene Assay, Expressing, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: Four-and-a-half LIM Domains 1 (FHL1) Protein Interacts with the Rho Guanine Nucleotide Exchange Factor PLEKHG2/FLJ00018 and Regulates Cell Morphogenesis

doi: 10.1074/jbc.M116.759571

Figure Lengend Snippet: PLEKHG2 and FLH1A were co-localized in Neuro-2a cells and regulated cell morphogenesis. A and D, Neuro-2a cells were co-transfected with Myc-tagged PLEKHG2 and FLAG-tagged FHL1A or FHL1B, as indicated. Cells were lysed 24 h after transfection, and PLEKHG2 was immunoprecipitated with anti-Myc antibody. Precipitated proteins were separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) or anti-FLAG antibody (for FHL1). B and E, Neuro-2a cells were co-transfected with pSRE.L-luciferase, pRL-SV40, PLEKHG2, FHL1A, FHL1B, and Gβγ, as indicated. Cells were lysed 24 h after transfection, and the effect of FHL1 protein on PLEKHG2-induced transcription was analyzed by a luciferase reporter gene assay. The experiment was performed in triplicate, and the values are the means ± S.D. (error bars). The data shown are representative of three independent experiments. C and F, Neuro-2a cells were co-transfected with PLEKHG2, FHL1A, FHL1B, and Gβγ, as indicated. Cells were lysed 24 h after transfection, and the lysate was separated by SDS-PAGE and immunoblotted with anti-Myc antibody (for PLEKHG2) and anti-FLAG antibody (for FHL1). PLEK2, PLEKHG2; TCL, total cell lysate; IP, immunoprecipitation. IB, immunoblotting. *, immunoglobulins precipitated from immunoprecipitation.

Article Snippet: The pSRE.L-luciferase reporter plasmid was purchased from Stratagene (Santa Clara, CA), and pRL-SV40 was purchased from Nippon Gene (Tokyo, Japan).

Techniques: Transfection, Immunoprecipitation, SDS Page, Luciferase, Reporter Gene Assay, Western Blot

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