Journal: Scientific Reports

Article Title: An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells

doi: 10.1038/s41598-021-86560-0

Figure Lengend Snippet: Bacterial strains recovered from the interior of B. rapa var. perviridis .

Article Snippet: BR1R-5 , Root , Proteobacteria , LC511710 , Pseudomonas agarici ATCC 25941 T (NR_115608.1) , 417 / 425 (98).

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Article Title: A Highly Selective PCR Protocol for Detecting 16S rRNA Genes of the Genus Pseudomonas (Sensu Stricto) in Environmental Samples

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Figure Lengend Snippet: Results of experimental specificity testing of pure cultures with the Ps-PCR primers

Article Snippet: P. agarici ATCC 25941* , B , Psm.B.

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Article Title: A Highly Selective PCR Protocol for Detecting 16S rRNA Genes of the Genus Pseudomonas (Sensu Stricto) in Environmental Samples

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Figure Lengend Snippet: PCR amplification and HaeIII RFLP patterns of pure cultures. PCR amplification (30 cycles) of 16S DNA coding for rRNA of pure cultures was performed either with a universal SSU primer set (a) or with the Ps-PCR primer set (b). (a1 and b1) Amplification products from cultures (lanes 1 to 18) and a negative control (neg) on 1% agarose gels. The molecular weight marker (MW) was λ HindIII. (a2 and b2) HaeIII RFLP patterns on 2% agarose gels. The molecular weight marker (MW) was ΦX174 HaeIII. The 18 cultures were “Pseudomonas” boreopolis (ATCC 3242) (lane 1), Burkholderia andropogonis (ATCC 23061) (lane 2), P. cichorii (ATCC 10857) (lane 3), P. syringae pv. syringae (Biolog 7350) (lane 4), “P.” floridana (ATCC 25616) (lane 5), “Pseudomonas” sp.-like group II (Biolog 1574) (lane 6), P. taetrolens (Biolog 8570) (lane 7), P. putida biotype B (ATCC 17527) (lane 8), P. synxantha (ATCC 9890) (lane 9), P. fragi (ATCC 4973) (lane 10), P. recinovorans (ATCC 14235) (lane 11), P. mendocina (ATCC 25411) (lane 12), P. agarici (ATCC 25941) (lane 13), P. fulva (ATCC 31418) (lane 14), P. stutzeri (ATCC 17588) (lane 15), P. alcaligenes (ATCC 14909) (lane 16), P. fluorescens biotype G (isolate 27cm) (lane 17), and P. marginalis (isolate A5P4C3) (lane 18). The Pseudomonas genus names that are in quotation marks are not Pseudomonas (sensu stricto) cultures.)

Article Snippet: P. agarici ATCC 25941* , B , Psm.B.

Techniques: Amplification, Negative Control, Molecular Weight, Marker