psci rs780094  (Thermo Fisher)


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    Structured Review

    Thermo Fisher psci rs780094
    Psci Rs780094, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psci rs780094/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psci rs780094 - by Bioz Stars, 2020-04
    86/100 stars

    Related Products / Commonly Used Together

    amplicons
    hpaii restriction endonuclease

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    Related Articles

    Amplification:

    Article Title: GCKR gene functional variants in type 2 diabetes and metabolic syndrome: do the rare variants associate with increased carotid intima-media thickness?
    Article Snippet: An MJ Research PTC-200 thermal cycler (Bio-Rad, Hercules, CA, USA) was used for the amplification. .. The amplicons were digested by HpaII restriction endonuclease (rs1260326) and PscI (rs780094) (Fermentas, Burlington, ON, Canada).

    Isolation:

    Article Title: GCKR gene functional variants in type 2 diabetes and metabolic syndrome: do the rare variants associate with increased carotid intima-media thickness?
    Article Snippet: Genotyping DNA was isolated from peripheral blood leukocytes by standard salting out method. .. The amplicons were digested by HpaII restriction endonuclease (rs1260326) and PscI (rs780094) (Fermentas, Burlington, ON, Canada).

    Size-exclusion Chromatography:

    Article Title: GCKR gene functional variants in type 2 diabetes and metabolic syndrome: do the rare variants associate with increased carotid intima-media thickness?
    Article Snippet: PCR conditions were the following: predenaturation at 96°C for 2 min; followed by 35 cycles of denaturation at 96°C for 20 sec (rs1260326), 30 cycles of denaturation at 96°C for 20 sec (rs780094); annealing at 60°C for 20 sec (rs1260326), and at 62°C for 30 sec (rs780094); primer extension for 30 sec at 72°C; and final extension at 72°C for 5 min. .. The amplicons were digested by HpaII restriction endonuclease (rs1260326) and PscI (rs780094) (Fermentas, Burlington, ON, Canada).

    Salting Out:

    Article Title: GCKR gene functional variants in type 2 diabetes and metabolic syndrome: do the rare variants associate with increased carotid intima-media thickness?
    Article Snippet: Genotyping DNA was isolated from peripheral blood leukocytes by standard salting out method. .. The amplicons were digested by HpaII restriction endonuclease (rs1260326) and PscI (rs780094) (Fermentas, Burlington, ON, Canada).

    Polymerase Chain Reaction:

    Article Title: GCKR gene functional variants in type 2 diabetes and metabolic syndrome: do the rare variants associate with increased carotid intima-media thickness?
    Article Snippet: PCR conditions were the following: predenaturation at 96°C for 2 min; followed by 35 cycles of denaturation at 96°C for 20 sec (rs1260326), 30 cycles of denaturation at 96°C for 20 sec (rs780094); annealing at 60°C for 20 sec (rs1260326), and at 62°C for 30 sec (rs780094); primer extension for 30 sec at 72°C; and final extension at 72°C for 5 min. .. The amplicons were digested by HpaII restriction endonuclease (rs1260326) and PscI (rs780094) (Fermentas, Burlington, ON, Canada).

    Activated Clotting Time Assay:

    Article Title: GCKR gene functional variants in type 2 diabetes and metabolic syndrome: do the rare variants associate with increased carotid intima-media thickness?
    Article Snippet: For polymerase chain reaction amplification, the following primers (MWG-Biotech AG, Ebersberg, Germany) were used: GCKR rs1260326: forward 5'-TGC AGA CTA TAG TGG AGC CG-3' and reverse 5'-CAT CAC ATG GCC ACT GCT TT-3'; GCKR rs780094: forward 5'-GAT TGT CTC AGG CAA ACC TGG TAG-3' and reverse 5'-CTA GGA GTG GTG GCA TAC ACC TG-3'. .. The amplicons were digested by HpaII restriction endonuclease (rs1260326) and PscI (rs780094) (Fermentas, Burlington, ON, Canada).

    Cellular Antioxidant Activity Assay:

    Article Title: GCKR gene functional variants in type 2 diabetes and metabolic syndrome: do the rare variants associate with increased carotid intima-media thickness?
    Article Snippet: For polymerase chain reaction amplification, the following primers (MWG-Biotech AG, Ebersberg, Germany) were used: GCKR rs1260326: forward 5'-TGC AGA CTA TAG TGG AGC CG-3' and reverse 5'-CAT CAC ATG GCC ACT GCT TT-3'; GCKR rs780094: forward 5'-GAT TGT CTC AGG CAA ACC TGG TAG-3' and reverse 5'-CTA GGA GTG GTG GCA TAC ACC TG-3'. .. The amplicons were digested by HpaII restriction endonuclease (rs1260326) and PscI (rs780094) (Fermentas, Burlington, ON, Canada).