prunus necrotic ringspot virus pnrsv  (ATCC)

Journal: Scientific Reports

Article Title: Rapid Detection of Prunus Necrotic Ringspot Virus by Reverse Transcription-cross-priming Amplification Coupled with Nucleic Acid Test Strip Cassette

doi: 10.1038/s41598-017-16536-6

Figure Lengend Snippet: Primer set screening for RT-CPA assay of PNRSV. ( A ) Reaction curves of real-time RT-CPA. Fluorescence was monitored every 60 s, and one cycle shown here represents 1 min. ( B ) Target sequence and primer location of the primer set G7. Nucleotide positions are based on the PNRSV coat protein (CP)-encoding gene (Genbank No: NC-004364.1). The cross primer (5′-TGTTCCACCTTATAGTCCTGTACTGTTATAGTCCGAATGA-3′) covers the PNRSV-CPF (5′ to 3′) and PNRSV-MBR (3′ to 5′) sequences.

Article Snippet: Prunus necrotic ringspot virus (PNRSV) , American Type Culture Collection (Manassas, VA, USA), ATCC (PV-552).

Techniques: Fluorescence, Sequencing

Journal: Scientific Reports

Article Title: Rapid Detection of Prunus Necrotic Ringspot Virus by Reverse Transcription-cross-priming Amplification Coupled with Nucleic Acid Test Strip Cassette

doi: 10.1038/s41598-017-16536-6

Figure Lengend Snippet: Specificity of RT-CPA-NATSC on the PNRSV detection. The reaction on PNRSV (lane 1) clearly showed both the test line and the control line, but only the control line were observed from the five common Prunus spp. pathogens, PPV, ASSVd, PLMVd, ApMV and ArMV (lane 2–6), as well as negative control and blank control (lane 7 and lane 8). Negative control, Totol RNA of healthy cucumber leaves; Blank control, RNase-free distilled water.

Article Snippet: Prunus necrotic ringspot virus (PNRSV) , American Type Culture Collection (Manassas, VA, USA), ATCC (PV-552).

Techniques: Control, Negative Control

Journal: Scientific Reports

Article Title: Rapid Detection of Prunus Necrotic Ringspot Virus by Reverse Transcription-cross-priming Amplification Coupled with Nucleic Acid Test Strip Cassette

doi: 10.1038/s41598-017-16536-6

Figure Lengend Snippet: Sensitivity of RT-CPA-NATSC on PNRSV detection. ( A ) Comparison of the sensitivities of RT-CPA-NATSC (the upper pannel) and RT-PCR (the lower pannel) on the PNRSV detection. Lane1–5 represent 10 −1 –10 −5 dilution of total RNA of the PNRSV-infected cucumber sample, respectively. ( B ) The detection limit of RT-CPA-NATSC on PNRSV. Lane 1–6 represent 50 −1 –50 −5 dilution of pPNRSV-CP, respectively. Both the test line and the control line were indicated in lane 1-5.

Article Snippet: Prunus necrotic ringspot virus (PNRSV) , American Type Culture Collection (Manassas, VA, USA), ATCC (PV-552).

Techniques: Comparison, Reverse Transcription Polymerase Chain Reaction, Infection, Control

Journal: Scientific Reports

Article Title: Rapid Detection of Prunus Necrotic Ringspot Virus by Reverse Transcription-cross-priming Amplification Coupled with Nucleic Acid Test Strip Cassette

doi: 10.1038/s41598-017-16536-6

Figure Lengend Snippet: Detection of PNRSV in field samples with RT-CPA-NATSC (the upper pannel) and RT-PCR (the lower panel). Positive control, Total RNA of PNRSV infected cucumber leaves; Negative control, Total RNA of healthy cherry leaves; S1-S5, Total RNA of samples 1-5.

Article Snippet: Prunus necrotic ringspot virus (PNRSV) , American Type Culture Collection (Manassas, VA, USA), ATCC (PV-552).

Techniques: Reverse Transcription Polymerase Chain Reaction, Positive Control, Infection, Negative Control

Journal: Scientific Reports

Article Title: Rapid Detection of Prunus Necrotic Ringspot Virus by Reverse Transcription-cross-priming Amplification Coupled with Nucleic Acid Test Strip Cassette

doi: 10.1038/s41598-017-16536-6

Figure Lengend Snippet: DAS-ELISA detection of PNRSV in field samples.

Article Snippet: Prunus necrotic ringspot virus (PNRSV) , American Type Culture Collection (Manassas, VA, USA), ATCC (PV-552).

Techniques: Positive Control, Negative Control

Journal: Scientific Reports

Article Title: Rapid Detection of Prunus Necrotic Ringspot Virus by Reverse Transcription-cross-priming Amplification Coupled with Nucleic Acid Test Strip Cassette

doi: 10.1038/s41598-017-16536-6

Figure Lengend Snippet: Viruses and viroids used for the developmention of RT-CPA-NATSC assay.

Article Snippet: Prunus necrotic ringspot virus (PNRSV) , American Type Culture Collection (Manassas, VA, USA), ATCC (PV-552).

Techniques: Virus

Journal: Viruses

Article Title: Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review

doi: 10.3390/v13030412

Figure Lengend Snippet: List of conventional PCR and RT-PCR delineate primers targeting different conserved region of viral genome sequences for identifying viral infections.

Article Snippet: Prunus , Mixed virus infections , Prunus necrotic ringspot virus (PNRSV) Prune dwarf virus (PDV) Apple mosaic virus (ApMV) American plum line pattern virus (APLPV) Ilarvirus-like RNA2 amplicon sequences , Illumina MiSeq , [ ] .

Techniques: Sequencing, Virus

Journal: Viruses

Article Title: Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review

doi: 10.3390/v13030412

Figure Lengend Snippet: Next-generation sequencing (second-generation sequencing platforms)-based identification of causative agents associated with the viral diseases of various crops.

Article Snippet: Prunus , Mixed virus infections , Prunus necrotic ringspot virus (PNRSV) Prune dwarf virus (PDV) Apple mosaic virus (ApMV) American plum line pattern virus (APLPV) Ilarvirus-like RNA2 amplicon sequences , Illumina MiSeq , [ ] .

Techniques: Next-Generation Sequencing, Sequencing, Virus, Variant Assay, Infection, Amplification