prset expression vector  (Thermo Fisher)


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    Name:
    pRSET A B C Bacterial Expression Vectors
    Description:
    The pRSET vector is designed for high level prokaryotic expression controlled by the strong bacteriophage T7 promoter Expression is induced by the production of T7 RNA polymerase in BL21 DE3 E coli These cells also produce T7 lysozyme to reduce basal expression of target genes The pRSET vector offers • Bacteriophage T7 promoter for high level expression• T7 gene 10 sequence to provide protein stability• N terminal polyhistidine 6xHis tag for rapid purification with nickel chelating resin and detection with an Anti HisG Antibody• N terminal Xpress epitope for detection with the Anti Xpress Antibody• Enterokinase cleavage site for removal of fusion tagA set of three vectors is provided A B and C Each has the N terminal tag coding sequence in a different reading frame relative to the multiple cloning site to simplify in frame cloning of your gene
    Catalog Number:
    v35120
    Price:
    None
    Applications:
    Bacterial Expression|Protein Biology|Protein Expression|T7 Bacterial Expression
    Category:
    DNA Vectors Clones Purified Nucleic Acids Libraries
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    Structured Review

    Thermo Fisher prset expression vector
    The pRSET vector is designed for high level prokaryotic expression controlled by the strong bacteriophage T7 promoter Expression is induced by the production of T7 RNA polymerase in BL21 DE3 E coli These cells also produce T7 lysozyme to reduce basal expression of target genes The pRSET vector offers • Bacteriophage T7 promoter for high level expression• T7 gene 10 sequence to provide protein stability• N terminal polyhistidine 6xHis tag for rapid purification with nickel chelating resin and detection with an Anti HisG Antibody• N terminal Xpress epitope for detection with the Anti Xpress Antibody• Enterokinase cleavage site for removal of fusion tagA set of three vectors is provided A B and C Each has the N terminal tag coding sequence in a different reading frame relative to the multiple cloning site to simplify in frame cloning of your gene
    https://www.bioz.com/result/prset expression vector/product/Thermo Fisher
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    prset expression vector - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Human protein Staufen-2 promotes HIV-1 proliferation by positively regulating RNA export activity of viral protein Rev
    Article Snippet: .. His-Rev-setB Full length cDNA of HIV-1 Rev was amplified from CMVsrev (gift from Prof. B. K. Felber, Center for Cancer Research, USA) using primers Rev-His-FP and Rev-His-RP (Additional file : Table S1) and cloned into BglII and NcoI sites of pRSET-B E.coli T7 Expression Vector (Invitrogen, USA) to generate hexa-histidine tag at the N-terminal. .. Rev-EGFP-C3 The Rev gene was amplified from His-Rev-setB construct using primers Rev-GFP-FP and Rev-GFP-RP (Additional file : Table S1) and cloned into BglII and SalI site of pEGFP-C3.

    Article Title: Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
    Article Snippet: .. The PCR product was digested with restriction enzymes and cloned into similarly digested pRSET-A bacterial expression vectors (Invitrogen). .. The plasmids were prepared in E. coli JM109 cells (Stratagene).

    Article Title: Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
    Article Snippet: .. The PCR products were digested with restriction enzymes and cloned into similarly digested pRSET-A bacterial expression vectors (Invitrogen). .. The plasmids were prepared in Escherichia coli JM109 cells (Promega).

    Article Title: Formation of Spindle Poles by Dynein/Dynactin-Dependent Transport of Numa
    Article Snippet: .. The dynamitin PCR product was cloned in the vector pCR-TM2.1, excised using EcoR1, and cloned into the bacterial expression vector pRSET A (both from Invitrogen). .. Bacterial fusion protein was isolated using 8M urea and dialyzed against PBS before the assay.

    Amplification:

    Article Title: Human protein Staufen-2 promotes HIV-1 proliferation by positively regulating RNA export activity of viral protein Rev
    Article Snippet: .. His-Rev-setB Full length cDNA of HIV-1 Rev was amplified from CMVsrev (gift from Prof. B. K. Felber, Center for Cancer Research, USA) using primers Rev-His-FP and Rev-His-RP (Additional file : Table S1) and cloned into BglII and NcoI sites of pRSET-B E.coli T7 Expression Vector (Invitrogen, USA) to generate hexa-histidine tag at the N-terminal. .. Rev-EGFP-C3 The Rev gene was amplified from His-Rev-setB construct using primers Rev-GFP-FP and Rev-GFP-RP (Additional file : Table S1) and cloned into BglII and SalI site of pEGFP-C3.

    Isolation:

    Article Title: Induction of Indoleamine 2, 3-Dioxygenase in Human Dendritic Cells by a Cholera Toxin B Subunit—Proinsulin Vaccine
    Article Snippet: .. Expression vector pRSET-CTB-INS was transformed into the E . coli producer strain BL21 (DE3) pLysS (Invitrogen, Carlsbad, CA) for production and isolation of milligram amounts of the CTB-INS protein for further experiments [ ]. .. Synthesis and isolation of CTB-INS fusion protein The E . coli strain BL21 transformed with pRSET-CTB-INS [ ] was grown overnight at 37°C in a 2.0 ml Luria Broth (LB) shake culture containing 100 μg/ml ampicillin for selection of transformed cells.

    Article Title: Suppression of Dendritic Cell Activation by Diabetes Autoantigens Linked to the Cholera Toxin B Subunit
    Article Snippet: .. The expression vector PRSET-CTB-INS containing the gene encoding the CTB-INS fusion protein was introduced into E. coli producer strain BL21 (DE3)pLysS (Invitrogen, Carlsbad, CA), for nickel affinity column isolation of the recombinant protein ( ). .. The CTB-INS transformed E. coli strain BL-21, was grown in 250 ml Luria Broth (LB) medium containing ampicillin (100mg/ml).

    Produced:

    Article Title: UBXN2A enhances CHIP‐mediated proteasomal degradation of oncoprotein mortalin‐2 in cancer cells
    Article Snippet: .. Recombinant (HIS)6 ‐UBXN2A was produced in BL21(DE3) Escherichia coli (BioLabs, Ipswich, MA, USA) using the pRSET C bacterial expression vector as described in the protocol provided by the manufacturer (Thermo Fisher Scientific, Waltham, MA, USA). .. Human‐UBXN2A was subcloned into the pRSET C vector (Novagen, Madison, WI, USA) to produce a recombinant UBXN2A with a polyhistidine (6xHIS) tag at the N terminus of UBXN2A.

    Affinity Column:

    Article Title: Suppression of Dendritic Cell Activation by Diabetes Autoantigens Linked to the Cholera Toxin B Subunit
    Article Snippet: .. The expression vector PRSET-CTB-INS containing the gene encoding the CTB-INS fusion protein was introduced into E. coli producer strain BL21 (DE3)pLysS (Invitrogen, Carlsbad, CA), for nickel affinity column isolation of the recombinant protein ( ). .. The CTB-INS transformed E. coli strain BL-21, was grown in 250 ml Luria Broth (LB) medium containing ampicillin (100mg/ml).

    Expressing:

    Article Title: Human protein Staufen-2 promotes HIV-1 proliferation by positively regulating RNA export activity of viral protein Rev
    Article Snippet: .. His-Rev-setB Full length cDNA of HIV-1 Rev was amplified from CMVsrev (gift from Prof. B. K. Felber, Center for Cancer Research, USA) using primers Rev-His-FP and Rev-His-RP (Additional file : Table S1) and cloned into BglII and NcoI sites of pRSET-B E.coli T7 Expression Vector (Invitrogen, USA) to generate hexa-histidine tag at the N-terminal. .. Rev-EGFP-C3 The Rev gene was amplified from His-Rev-setB construct using primers Rev-GFP-FP and Rev-GFP-RP (Additional file : Table S1) and cloned into BglII and SalI site of pEGFP-C3.

    Article Title: Induction of Indoleamine 2, 3-Dioxygenase in Human Dendritic Cells by a Cholera Toxin B Subunit—Proinsulin Vaccine
    Article Snippet: .. Expression vector pRSET-CTB-INS was transformed into the E . coli producer strain BL21 (DE3) pLysS (Invitrogen, Carlsbad, CA) for production and isolation of milligram amounts of the CTB-INS protein for further experiments [ ]. .. Synthesis and isolation of CTB-INS fusion protein The E . coli strain BL21 transformed with pRSET-CTB-INS [ ] was grown overnight at 37°C in a 2.0 ml Luria Broth (LB) shake culture containing 100 μg/ml ampicillin for selection of transformed cells.

    Article Title: Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
    Article Snippet: .. The PCR product was digested with restriction enzymes and cloned into similarly digested pRSET-A bacterial expression vectors (Invitrogen). .. The plasmids were prepared in E. coli JM109 cells (Stratagene).

    Article Title: GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28
    Article Snippet: .. The PCR product was digested and subcloned into the pRSET–C bacterial expression vector (Invitrogen) to obtain a His6 N–terminal-tagged recombinant GATE–16 protein. .. The pRSET–C plasmid containing GATE–16 was transformed into E.coli BL–21.

    Article Title: UBXN2A enhances CHIP‐mediated proteasomal degradation of oncoprotein mortalin‐2 in cancer cells
    Article Snippet: .. Recombinant (HIS)6 ‐UBXN2A was produced in BL21(DE3) Escherichia coli (BioLabs, Ipswich, MA, USA) using the pRSET C bacterial expression vector as described in the protocol provided by the manufacturer (Thermo Fisher Scientific, Waltham, MA, USA). .. Human‐UBXN2A was subcloned into the pRSET C vector (Novagen, Madison, WI, USA) to produce a recombinant UBXN2A with a polyhistidine (6xHIS) tag at the N terminus of UBXN2A.

    Article Title: Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
    Article Snippet: .. The PCR products were digested with restriction enzymes and cloned into similarly digested pRSET-A bacterial expression vectors (Invitrogen). .. The plasmids were prepared in Escherichia coli JM109 cells (Promega).

    Article Title: Formation of Spindle Poles by Dynein/Dynactin-Dependent Transport of Numa
    Article Snippet: .. The dynamitin PCR product was cloned in the vector pCR-TM2.1, excised using EcoR1, and cloned into the bacterial expression vector pRSET A (both from Invitrogen). .. Bacterial fusion protein was isolated using 8M urea and dialyzed against PBS before the assay.

    Article Title: Suppression of Dendritic Cell Activation by Diabetes Autoantigens Linked to the Cholera Toxin B Subunit
    Article Snippet: .. The expression vector PRSET-CTB-INS containing the gene encoding the CTB-INS fusion protein was introduced into E. coli producer strain BL21 (DE3)pLysS (Invitrogen, Carlsbad, CA), for nickel affinity column isolation of the recombinant protein ( ). .. The CTB-INS transformed E. coli strain BL-21, was grown in 250 ml Luria Broth (LB) medium containing ampicillin (100mg/ml).

    CtB Assay:

    Article Title: Induction of Indoleamine 2, 3-Dioxygenase in Human Dendritic Cells by a Cholera Toxin B Subunit—Proinsulin Vaccine
    Article Snippet: .. Expression vector pRSET-CTB-INS was transformed into the E . coli producer strain BL21 (DE3) pLysS (Invitrogen, Carlsbad, CA) for production and isolation of milligram amounts of the CTB-INS protein for further experiments [ ]. .. Synthesis and isolation of CTB-INS fusion protein The E . coli strain BL21 transformed with pRSET-CTB-INS [ ] was grown overnight at 37°C in a 2.0 ml Luria Broth (LB) shake culture containing 100 μg/ml ampicillin for selection of transformed cells.

    Article Title: Suppression of Dendritic Cell Activation by Diabetes Autoantigens Linked to the Cholera Toxin B Subunit
    Article Snippet: .. The expression vector PRSET-CTB-INS containing the gene encoding the CTB-INS fusion protein was introduced into E. coli producer strain BL21 (DE3)pLysS (Invitrogen, Carlsbad, CA), for nickel affinity column isolation of the recombinant protein ( ). .. The CTB-INS transformed E. coli strain BL-21, was grown in 250 ml Luria Broth (LB) medium containing ampicillin (100mg/ml).

    Polymerase Chain Reaction:

    Article Title: Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
    Article Snippet: .. The PCR product was digested with restriction enzymes and cloned into similarly digested pRSET-A bacterial expression vectors (Invitrogen). .. The plasmids were prepared in E. coli JM109 cells (Stratagene).

    Article Title: GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28
    Article Snippet: .. The PCR product was digested and subcloned into the pRSET–C bacterial expression vector (Invitrogen) to obtain a His6 N–terminal-tagged recombinant GATE–16 protein. .. The pRSET–C plasmid containing GATE–16 was transformed into E.coli BL–21.

    Article Title: Dopamine D1A directly interacts with otoferlin synaptic pathway proteins: Ca2+ and phosphorylation underlie an NSF-to-AP2mu1 molecular switch
    Article Snippet: .. The PCR products were digested with restriction enzymes and cloned into similarly digested pRSET-A bacterial expression vectors (Invitrogen). .. The plasmids were prepared in Escherichia coli JM109 cells (Promega).

    Article Title: Formation of Spindle Poles by Dynein/Dynactin-Dependent Transport of Numa
    Article Snippet: .. The dynamitin PCR product was cloned in the vector pCR-TM2.1, excised using EcoR1, and cloned into the bacterial expression vector pRSET A (both from Invitrogen). .. Bacterial fusion protein was isolated using 8M urea and dialyzed against PBS before the assay.

    Transformation Assay:

    Article Title: Induction of Indoleamine 2, 3-Dioxygenase in Human Dendritic Cells by a Cholera Toxin B Subunit—Proinsulin Vaccine
    Article Snippet: .. Expression vector pRSET-CTB-INS was transformed into the E . coli producer strain BL21 (DE3) pLysS (Invitrogen, Carlsbad, CA) for production and isolation of milligram amounts of the CTB-INS protein for further experiments [ ]. .. Synthesis and isolation of CTB-INS fusion protein The E . coli strain BL21 transformed with pRSET-CTB-INS [ ] was grown overnight at 37°C in a 2.0 ml Luria Broth (LB) shake culture containing 100 μg/ml ampicillin for selection of transformed cells.

    Recombinant:

    Article Title: GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28
    Article Snippet: .. The PCR product was digested and subcloned into the pRSET–C bacterial expression vector (Invitrogen) to obtain a His6 N–terminal-tagged recombinant GATE–16 protein. .. The pRSET–C plasmid containing GATE–16 was transformed into E.coli BL–21.

    Article Title: UBXN2A enhances CHIP‐mediated proteasomal degradation of oncoprotein mortalin‐2 in cancer cells
    Article Snippet: .. Recombinant (HIS)6 ‐UBXN2A was produced in BL21(DE3) Escherichia coli (BioLabs, Ipswich, MA, USA) using the pRSET C bacterial expression vector as described in the protocol provided by the manufacturer (Thermo Fisher Scientific, Waltham, MA, USA). .. Human‐UBXN2A was subcloned into the pRSET C vector (Novagen, Madison, WI, USA) to produce a recombinant UBXN2A with a polyhistidine (6xHIS) tag at the N terminus of UBXN2A.

    Article Title: Suppression of Dendritic Cell Activation by Diabetes Autoantigens Linked to the Cholera Toxin B Subunit
    Article Snippet: .. The expression vector PRSET-CTB-INS containing the gene encoding the CTB-INS fusion protein was introduced into E. coli producer strain BL21 (DE3)pLysS (Invitrogen, Carlsbad, CA), for nickel affinity column isolation of the recombinant protein ( ). .. The CTB-INS transformed E. coli strain BL-21, was grown in 250 ml Luria Broth (LB) medium containing ampicillin (100mg/ml).

    Plasmid Preparation:

    Article Title: Human protein Staufen-2 promotes HIV-1 proliferation by positively regulating RNA export activity of viral protein Rev
    Article Snippet: .. His-Rev-setB Full length cDNA of HIV-1 Rev was amplified from CMVsrev (gift from Prof. B. K. Felber, Center for Cancer Research, USA) using primers Rev-His-FP and Rev-His-RP (Additional file : Table S1) and cloned into BglII and NcoI sites of pRSET-B E.coli T7 Expression Vector (Invitrogen, USA) to generate hexa-histidine tag at the N-terminal. .. Rev-EGFP-C3 The Rev gene was amplified from His-Rev-setB construct using primers Rev-GFP-FP and Rev-GFP-RP (Additional file : Table S1) and cloned into BglII and SalI site of pEGFP-C3.

    Article Title: Induction of Indoleamine 2, 3-Dioxygenase in Human Dendritic Cells by a Cholera Toxin B Subunit—Proinsulin Vaccine
    Article Snippet: .. Expression vector pRSET-CTB-INS was transformed into the E . coli producer strain BL21 (DE3) pLysS (Invitrogen, Carlsbad, CA) for production and isolation of milligram amounts of the CTB-INS protein for further experiments [ ]. .. Synthesis and isolation of CTB-INS fusion protein The E . coli strain BL21 transformed with pRSET-CTB-INS [ ] was grown overnight at 37°C in a 2.0 ml Luria Broth (LB) shake culture containing 100 μg/ml ampicillin for selection of transformed cells.

    Article Title: GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28
    Article Snippet: .. The PCR product was digested and subcloned into the pRSET–C bacterial expression vector (Invitrogen) to obtain a His6 N–terminal-tagged recombinant GATE–16 protein. .. The pRSET–C plasmid containing GATE–16 was transformed into E.coli BL–21.

    Article Title: UBXN2A enhances CHIP‐mediated proteasomal degradation of oncoprotein mortalin‐2 in cancer cells
    Article Snippet: .. Recombinant (HIS)6 ‐UBXN2A was produced in BL21(DE3) Escherichia coli (BioLabs, Ipswich, MA, USA) using the pRSET C bacterial expression vector as described in the protocol provided by the manufacturer (Thermo Fisher Scientific, Waltham, MA, USA). .. Human‐UBXN2A was subcloned into the pRSET C vector (Novagen, Madison, WI, USA) to produce a recombinant UBXN2A with a polyhistidine (6xHIS) tag at the N terminus of UBXN2A.

    Article Title: Formation of Spindle Poles by Dynein/Dynactin-Dependent Transport of Numa
    Article Snippet: .. The dynamitin PCR product was cloned in the vector pCR-TM2.1, excised using EcoR1, and cloned into the bacterial expression vector pRSET A (both from Invitrogen). .. Bacterial fusion protein was isolated using 8M urea and dialyzed against PBS before the assay.

    Article Title: Suppression of Dendritic Cell Activation by Diabetes Autoantigens Linked to the Cholera Toxin B Subunit
    Article Snippet: .. The expression vector PRSET-CTB-INS containing the gene encoding the CTB-INS fusion protein was introduced into E. coli producer strain BL21 (DE3)pLysS (Invitrogen, Carlsbad, CA), for nickel affinity column isolation of the recombinant protein ( ). .. The CTB-INS transformed E. coli strain BL-21, was grown in 250 ml Luria Broth (LB) medium containing ampicillin (100mg/ml).

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