proton sequencer supplementary  (Thermo Fisher)


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    Name:
    Ion Proton System
    Description:
    The Ion Proton System is the first benchtop sequencing system capable of human scale genome exome or transcriptome sequencing in a few hours with DNA to variants called in a single day The system combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data By leveraging the exponential improvements in the semiconductor industry known as Moore s Law the Ion Proton Sequencing System provides an unprecedented level of scalability and flexibility to support a broad range of high throughput sequencing applications ranging from human scale genome to exome to transcriptome sequencing The system s use of the simplest natural sequencing chemistry eliminates the need for expensive optics and complex sequencing chemistries resulting in a highly affordable sequencing system to own and operate Real time direct electrical detection of sequencing combined with the enormous amount of computing power in both the Ion Proton Sequencer and Ion Proton Torrent Server enables generation of high quality sequencing results from DNA library to variants in a single day Features of the Ion Proton System • Fastest high throughput next generation sequencing workflow with the fastest sequencing run times of 2 4 hours on the Ion PI Chip • Highest number of complete sequencing runs per week with a simple and automated workflow when used with the Ion OneTouch 2 System • Simple semiconductor sequencing workflows with robust and simple hardware that can be relied upon no cameras no optics and no lasers • Scalable high throughput sequencing capabilities enabling up to 200 base single reads and flexible library options e g fragment gDNA targeted exome RNA • Small benchtop footprint and mounting capability with optional rack two Ion Proton systems per rack to deliver the only benchtop genome center • Low cost platform and attractively priced semiconductor chips and reagents for a range of applications • Proven Ion semiconductor sequencing requires no complex optics and employs natural nucleotides to deliver highly accurate variant detection uniformity of coverage and sensitivity to detect low frequency variants • Range of fast and simple library solutions and kits with low input requirements for a variety of applications such as genomic sequencing exome sequencing sequencing sets of genes and RNA sequencing • Complete end to end solution from base calls to variants with the Proton Torrent Server and Torrent Suite Software v3 0 • Simple and integrated tools for tertiary data analysis with Ion Reporter Software for DNA variation analysis across single paired or trio samples With the availability of the groundbreaking Ion Proton System rapid high throughput sequencing is finally accessible by all laboratories Affordable Sequencing For Nearly Every LabPrevious sequencing technologies have relied upon optical systems as bridges between the chemical and digital forms of sequence information These systems have added tremendous costs to sequencers relegating them to only the largest laboratories By eliminating the need for the optical system the Ion Proton System provides high throughput sequencing that is simpler faster more cost effective and more scalable than any other technology available With a benchtop footprint low cost sequencing reagents and a simple touchscreen user interface the Ion Proton System brings high throughput next generation sequencing to nearly every lab Ion Power PostLight Sequencing Produces Bits From BasesThe sequencing technology underlying the Ion Proton System exploits a well characterized biochemical process When a nucleotide is incorporated into a strand of DNA by a polymerase a hydrogen ion H is released as a byproduct This hydrogen ion carries a charge that the Proton System s ion sensor essentially the world s smallest solid state pH meter can detect As the sequencer floods the chip with one nucleotide after another any nucleotide added to a DNA template will be detected as a voltage change and the Ion Proton System will call the base If a nucleotide is not a match for a particular template no voltage change will be detected and no base will be called for that template Massively Parallel Sequencing On Your BenchA principal component of the Ion Proton System is the sequencing chip The Ion PI Chip incorporates an extremely dense array of 165 million micro machined wells married to our proprietary ion sensor Each well contains a different DNA template allowing massively parallel sequencing With the Ion PI Chip 165 million wells the Ion Proton System enables a range of high throughput genomic applications from human scale exome sequencing to whole transcriptome sequencing The Right Tool To Get Results Easily Reliably and QuicklyBecause it detects nucleotide incorporation without the use of light the Ion Proton System uses the simplest sequencing chemistry possible natural nucleotides There is no need for expensive and error prone modified bases enzymatic cascades chemiluminescence or fluorescence Direct detection also means the incorporation of each nucleotide is recorded in seconds As a result you can do an entire sequencing run typically in a few hours Services and Support Service and support plans comprehensive plans to maximize instrument performance and ensure availability of critical systems Qualification services document and ensure instrument is installed operating and performing to manufacturer s specifications Training courses for experimental design data analysis troubleshooting applications and instrument operation Bioinformatics tailored support for NGS informatics execution software use data analysis and downstream applications DataSafe Solution storage and back up strategy design hardware and support for all lab data
    Catalog Number:
    4476610
    Price:
    None
    Applications:
    DNA Sequencing|Epigenetic Sequencing|Fragment Analysis|Genotyping & Genomic Profiling|Ion Torrent™ Next-Generation Sequencing|Ion Torrent™ Systems & Accessories|Mitochondrial Sequencing|RNA Sequencing|SNP Genotyping by Fragment-Analysis|Sequencing|Small RNA & miRNA Sequencing|Methylation Analysis by Sequencing|Whole Genome Sequencing|Whole Transcriptome Sequencing|SNP Genotyping|Gene Expression Analysis & Genotyping|ChIP Sequencing|De Novo Sequencing|Gene Expression Profiling by Sequencing
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    Instruments and Equipment
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    Structured Review

    Thermo Fisher proton sequencer supplementary
    The Ion Proton System is the first benchtop sequencing system capable of human scale genome exome or transcriptome sequencing in a few hours with DNA to variants called in a single day The system combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data By leveraging the exponential improvements in the semiconductor industry known as Moore s Law the Ion Proton Sequencing System provides an unprecedented level of scalability and flexibility to support a broad range of high throughput sequencing applications ranging from human scale genome to exome to transcriptome sequencing The system s use of the simplest natural sequencing chemistry eliminates the need for expensive optics and complex sequencing chemistries resulting in a highly affordable sequencing system to own and operate Real time direct electrical detection of sequencing combined with the enormous amount of computing power in both the Ion Proton Sequencer and Ion Proton Torrent Server enables generation of high quality sequencing results from DNA library to variants in a single day Features of the Ion Proton System • Fastest high throughput next generation sequencing workflow with the fastest sequencing run times of 2 4 hours on the Ion PI Chip • Highest number of complete sequencing runs per week with a simple and automated workflow when used with the Ion OneTouch 2 System • Simple semiconductor sequencing workflows with robust and simple hardware that can be relied upon no cameras no optics and no lasers • Scalable high throughput sequencing capabilities enabling up to 200 base single reads and flexible library options e g fragment gDNA targeted exome RNA • Small benchtop footprint and mounting capability with optional rack two Ion Proton systems per rack to deliver the only benchtop genome center • Low cost platform and attractively priced semiconductor chips and reagents for a range of applications • Proven Ion semiconductor sequencing requires no complex optics and employs natural nucleotides to deliver highly accurate variant detection uniformity of coverage and sensitivity to detect low frequency variants • Range of fast and simple library solutions and kits with low input requirements for a variety of applications such as genomic sequencing exome sequencing sequencing sets of genes and RNA sequencing • Complete end to end solution from base calls to variants with the Proton Torrent Server and Torrent Suite Software v3 0 • Simple and integrated tools for tertiary data analysis with Ion Reporter Software for DNA variation analysis across single paired or trio samples With the availability of the groundbreaking Ion Proton System rapid high throughput sequencing is finally accessible by all laboratories Affordable Sequencing For Nearly Every LabPrevious sequencing technologies have relied upon optical systems as bridges between the chemical and digital forms of sequence information These systems have added tremendous costs to sequencers relegating them to only the largest laboratories By eliminating the need for the optical system the Ion Proton System provides high throughput sequencing that is simpler faster more cost effective and more scalable than any other technology available With a benchtop footprint low cost sequencing reagents and a simple touchscreen user interface the Ion Proton System brings high throughput next generation sequencing to nearly every lab Ion Power PostLight Sequencing Produces Bits From BasesThe sequencing technology underlying the Ion Proton System exploits a well characterized biochemical process When a nucleotide is incorporated into a strand of DNA by a polymerase a hydrogen ion H is released as a byproduct This hydrogen ion carries a charge that the Proton System s ion sensor essentially the world s smallest solid state pH meter can detect As the sequencer floods the chip with one nucleotide after another any nucleotide added to a DNA template will be detected as a voltage change and the Ion Proton System will call the base If a nucleotide is not a match for a particular template no voltage change will be detected and no base will be called for that template Massively Parallel Sequencing On Your BenchA principal component of the Ion Proton System is the sequencing chip The Ion PI Chip incorporates an extremely dense array of 165 million micro machined wells married to our proprietary ion sensor Each well contains a different DNA template allowing massively parallel sequencing With the Ion PI Chip 165 million wells the Ion Proton System enables a range of high throughput genomic applications from human scale exome sequencing to whole transcriptome sequencing The Right Tool To Get Results Easily Reliably and QuicklyBecause it detects nucleotide incorporation without the use of light the Ion Proton System uses the simplest sequencing chemistry possible natural nucleotides There is no need for expensive and error prone modified bases enzymatic cascades chemiluminescence or fluorescence Direct detection also means the incorporation of each nucleotide is recorded in seconds As a result you can do an entire sequencing run typically in a few hours Services and Support Service and support plans comprehensive plans to maximize instrument performance and ensure availability of critical systems Qualification services document and ensure instrument is installed operating and performing to manufacturer s specifications Training courses for experimental design data analysis troubleshooting applications and instrument operation Bioinformatics tailored support for NGS informatics execution software use data analysis and downstream applications DataSafe Solution storage and back up strategy design hardware and support for all lab data
    https://www.bioz.com/result/proton sequencer supplementary/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    proton sequencer supplementary - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Differential Effects of Vitamins A and D on the Transcriptional Landscape of Human Monocytes during Infection
    Article Snippet: .. Library templates were clonally amplified on Ion Sphere particles using the Ion PI Hi-Q Chef Kit and Ion Chef instrument (Thermo Fisher Scientific, USA), loaded onto Ion PI Chips and sequenced on an Ion Proton Sequencer (Thermo Fisher Scientific, USA). .. The raw sequence data in fastq format are stored in the Sequence Read Archive (SRA) at National Center for Biotechnology Information (NCBI) and can be accessed at NCBI homepage ( https://www.ncbi.nlm.nih.gov/ ; accession number: SRP076532).

    RNA Sequencing Assay:

    Article Title: NuA4 Lysine Acetyltransferase Complex Contributes to Phospholipid Homeostasis in Saccharomyces cerevisiae
    Article Snippet: .. All six RNA-seq libraries were multiplexed and sequenced on the Life Technologies Ion Torrent Proton platform using a single Ion Proton Chip. ..

    Next-Generation Sequencing:

    Article Title: Preoperative detection of RAS mutation may guide extent of thyroidectomy
    Article Snippet: .. Samples were tested using NGS on the Ion Torrent PGM or Ion Proton (Life Technologies, Carlsbad, CA) and the ThyroSeq panel, as previously described. .. The analytic sensitivity of the assay was ~3% mutant alleles, but the clinical sensitivity was set at mutational allelic frequency (AF) ≥10% (eg, 10% of tested cells contain a mutant allele).

    Sequencing:

    Article Title: Identification of genetic linkage group 1-linked sequences in Japanese eel (Anguilla japonica) by single chromosome sorting and sequencing
    Article Snippet: .. Template samples were sequenced on Ion P1 Chip v2 using the Ion Proton platform with an Ion P1 Sequencing 200 kit v3 (Ion Torrent-Thermo Fisher Scientific). .. Data from the Ion Proton runs were processed using Ion Torrent Suite 4.2.1 software to generate sequenced reads.

    Article Title: Opposing effects of cancer type-specific SPOP mutations on BET protein degradation and sensitivity to BET inhibitors
    Article Snippet: .. Enriched samples were then loaded on an Ion PI™ Chip v3 (Thermo Fisher Scientific/Life Technologies) and sequenced on the Ion Proton™ System using Ion PI™ Hi-Q™ Sequencing 200 Kit (Thermo Fisher Scientific/Life Technologies). ..

    Article Title: The IL-1B Gene Polymorphisms rs16944 and rs1143627 Contribute to an Increased Risk of Coronary Artery Lesions in Southern Chinese Children with Kawasaki Disease
    Article Snippet: .. Briefly, high-quality genomic DNA samples were genotyped by PCR using multiple gene-specific primer pairs to enrich the specific SNPs and indexing primers to enable massive parallel sequencing on the Ion Proton System (Life Technologies). ..

    Article Title: The GTPase domain of gamma-tubulin is required for normal mitochondrial function and spatial organization
    Article Snippet: .. The pooled samples were loaded on Ion PI™ v3 chips and sequenced on the Ion Proton™ System using the Ion PI™ Hi-Q Sequencing 200 Kit chemistry (200 bp read length, ThermoFisher). .. RNA-seq and ChIP-seq analysis RNA-seq reads were mapped to the reference genome (including the mitochondria genome) using TopHat (version 2.0.9, parameters: read mismatches: 2, -read gap length: 2, -max insertion length: 3, -max deletion length: 3).

    Article Title: Limits and patterns of cytomegalovirus genomic diversity in humans
    Article Snippet: .. Pooled DNA was then prepared for sequencing on the Illumina HiSeq2000 as described previously ( ) or for sequencing on the ION proton sequencer (Life Technologies) using the manufacturer’s standard protocols and reagents. .. Samples were labeled with DNA barcodes and aggregated into libraries that generally included four to six samples.

    Polymerase Chain Reaction:

    Article Title: The IL-1B Gene Polymorphisms rs16944 and rs1143627 Contribute to an Increased Risk of Coronary Artery Lesions in Southern Chinese Children with Kawasaki Disease
    Article Snippet: .. Briefly, high-quality genomic DNA samples were genotyped by PCR using multiple gene-specific primer pairs to enrich the specific SNPs and indexing primers to enable massive parallel sequencing on the Ion Proton System (Life Technologies). ..

    Chromatin Immunoprecipitation:

    Article Title: Identification of genetic linkage group 1-linked sequences in Japanese eel (Anguilla japonica) by single chromosome sorting and sequencing
    Article Snippet: .. Template samples were sequenced on Ion P1 Chip v2 using the Ion Proton platform with an Ion P1 Sequencing 200 kit v3 (Ion Torrent-Thermo Fisher Scientific). .. Data from the Ion Proton runs were processed using Ion Torrent Suite 4.2.1 software to generate sequenced reads.

    Article Title: NuA4 Lysine Acetyltransferase Complex Contributes to Phospholipid Homeostasis in Saccharomyces cerevisiae
    Article Snippet: .. All six RNA-seq libraries were multiplexed and sequenced on the Life Technologies Ion Torrent Proton platform using a single Ion Proton Chip. ..

    Article Title: Opposing effects of cancer type-specific SPOP mutations on BET protein degradation and sensitivity to BET inhibitors
    Article Snippet: .. Enriched samples were then loaded on an Ion PI™ Chip v3 (Thermo Fisher Scientific/Life Technologies) and sequenced on the Ion Proton™ System using Ion PI™ Hi-Q™ Sequencing 200 Kit (Thermo Fisher Scientific/Life Technologies). ..

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    Thermo Fisher 6000x average read depth
    6000x Average Read Depth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6000x average read depth/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    6000x average read depth - by Bioz Stars, 2020-09
    84/100 stars
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