protia sigma kit  (Millipore)


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    Name:
    ProteoPrep Immunoaffinity Albumin IgG Depletion Kit
    Description:
    The ProteoPrep Immunoaffinity medium in the prepacked spin columns is a mixture of two beaded media containing recombinantly expressed small single chain antibody ligands resulting in low non specific binding and high capacity This kit is also effective in depleting albumin and IgG from mouse and guinea pig serum
    Catalog Number:
    protia
    Price:
    None
    Applications:
    The ProteoPrep(R) Immunoaffinity Albumin and IgG Depletion Kit has been designed to specifically remove albumin and IgG from human serum (25-50muL) in preparation of samples for proteomics analysis, two-dimensional electrophoresis (2DE) or liquid chromatography (LC).
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    Structured Review

    Millipore protia sigma kit
    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and <t>PROTIA-Sigma)</t> were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    The ProteoPrep Immunoaffinity medium in the prepacked spin columns is a mixture of two beaded media containing recombinantly expressed small single chain antibody ligands resulting in low non specific binding and high capacity This kit is also effective in depleting albumin and IgG from mouse and guinea pig serum
    https://www.bioz.com/result/protia sigma kit/product/Millipore
    Average 96 stars, based on 60 article reviews
    Price from $9.99 to $1999.99
    protia sigma kit - by Bioz Stars, 2020-09
    96/100 stars

    Images

    1) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    2) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Two Dimensional Electrophoresis Gel image of crude and depleted umbilical cord serum samples . For each gel, 150 μg of protein was separated over pH range 4-7 (24 cm strips) and 12.5% SDS-polyacrylamide gel. The gel was visualized by silver staining. The Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) Kit was used to deplete albumin and IgG in the umbilical cord serum. Removal of these proteins improved the resolution in the albumin and IgG areas on the gel and increased the intensity of low abundance proteins. A) Two Dimension Electrophoresis Gel image of crude umbilical cord serum. B) Two Dimension Electrophoresis Gel image of depleted umbilical cord serum by Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA). Circles: regions of gel containing albumin, heavy and light chain of IgG before (panel A) and after depletion (panel B). Rectangle: used to highlight different protein patterns in the two groups, indicating a large number of protein spots that emerged in the depleted gel.
    Figure Legend Snippet: Two Dimensional Electrophoresis Gel image of crude and depleted umbilical cord serum samples . For each gel, 150 μg of protein was separated over pH range 4-7 (24 cm strips) and 12.5% SDS-polyacrylamide gel. The gel was visualized by silver staining. The Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) Kit was used to deplete albumin and IgG in the umbilical cord serum. Removal of these proteins improved the resolution in the albumin and IgG areas on the gel and increased the intensity of low abundance proteins. A) Two Dimension Electrophoresis Gel image of crude umbilical cord serum. B) Two Dimension Electrophoresis Gel image of depleted umbilical cord serum by Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA). Circles: regions of gel containing albumin, heavy and light chain of IgG before (panel A) and after depletion (panel B). Rectangle: used to highlight different protein patterns in the two groups, indicating a large number of protein spots that emerged in the depleted gel.

    Techniques Used: Electrophoresis, Silver Staining

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    Identification of protein spots unique to depleted umbilical cord serum gels by the Immunoaffinity Albumin and IgG Depletion kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) . Circles: Ten spots unique to the depleted gels were selected for MALDI-TOF/TOF MS identification. Gel image is the same as in Fig 2B. The numbers correspond to the protein numbers in Table 2.
    Figure Legend Snippet: Identification of protein spots unique to depleted umbilical cord serum gels by the Immunoaffinity Albumin and IgG Depletion kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) . Circles: Ten spots unique to the depleted gels were selected for MALDI-TOF/TOF MS identification. Gel image is the same as in Fig 2B. The numbers correspond to the protein numbers in Table 2.

    Techniques Used: Mass Spectrometry

    3) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    4) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    5) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    6) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    7) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    8) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    9) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    10) Product Images from "Purification and Functional Characterisation of Rhinocerase, a Novel Serine Protease from the Venom of Bitis gabonica rhinoceros"

    Article Title: Purification and Functional Characterisation of Rhinocerase, a Novel Serine Protease from the Venom of Bitis gabonica rhinoceros

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0009687

    Fibrinogenolytic activity of rhinocerase. Coomassie brilliant blue stained SDS-PAGE gels containing samples taken at different time intervals during the incubation of 100 µg of rhinocerase with 400 µl (5 mg/ml) of plasminogen-free fibrinogen in 0.05M Tris-HCl; 0.1M NaCl, pH 7.8 or 400 µl of human plasma in the presence and absence of 20 mM EDTA. The numbers at the top of each lane represent the time (in minutes) when samples were taken during the digestion. A . The degradation profile of plasminogen-free fibrinogen by rhinocerase. α, β and γ represent the three chains of fibrinogen and A and B represent the degradation products. The position of rhinocerase has been labelled on the image. B . The degradation profile of fibrinogen present in human plasma after removal of albumin and IgG by Proteoprep immunoaffinity albumin and IgG depletion columns. The position of the α chain of fibrinogen is marked with an arrow. Data are representative of three separate experiments.
    Figure Legend Snippet: Fibrinogenolytic activity of rhinocerase. Coomassie brilliant blue stained SDS-PAGE gels containing samples taken at different time intervals during the incubation of 100 µg of rhinocerase with 400 µl (5 mg/ml) of plasminogen-free fibrinogen in 0.05M Tris-HCl; 0.1M NaCl, pH 7.8 or 400 µl of human plasma in the presence and absence of 20 mM EDTA. The numbers at the top of each lane represent the time (in minutes) when samples were taken during the digestion. A . The degradation profile of plasminogen-free fibrinogen by rhinocerase. α, β and γ represent the three chains of fibrinogen and A and B represent the degradation products. The position of rhinocerase has been labelled on the image. B . The degradation profile of fibrinogen present in human plasma after removal of albumin and IgG by Proteoprep immunoaffinity albumin and IgG depletion columns. The position of the α chain of fibrinogen is marked with an arrow. Data are representative of three separate experiments.

    Techniques Used: Activity Assay, Staining, SDS Page, Incubation

    11) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    12) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    13) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    14) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    15) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Two Dimensional Electrophoresis Gel image of crude and depleted umbilical cord serum samples . For each gel, 150 μg of protein was separated over pH range 4-7 (24 cm strips) and 12.5% SDS-polyacrylamide gel. The gel was visualized by silver staining. The Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) Kit was used to deplete albumin and IgG in the umbilical cord serum. Removal of these proteins improved the resolution in the albumin and IgG areas on the gel and increased the intensity of low abundance proteins. A) Two Dimension Electrophoresis Gel image of crude umbilical cord serum. B) Two Dimension Electrophoresis Gel image of depleted umbilical cord serum by Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA). Circles: regions of gel containing albumin, heavy and light chain of IgG before (panel A) and after depletion (panel B). Rectangle: used to highlight different protein patterns in the two groups, indicating a large number of protein spots that emerged in the depleted gel.
    Figure Legend Snippet: Two Dimensional Electrophoresis Gel image of crude and depleted umbilical cord serum samples . For each gel, 150 μg of protein was separated over pH range 4-7 (24 cm strips) and 12.5% SDS-polyacrylamide gel. The gel was visualized by silver staining. The Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) Kit was used to deplete albumin and IgG in the umbilical cord serum. Removal of these proteins improved the resolution in the albumin and IgG areas on the gel and increased the intensity of low abundance proteins. A) Two Dimension Electrophoresis Gel image of crude umbilical cord serum. B) Two Dimension Electrophoresis Gel image of depleted umbilical cord serum by Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA). Circles: regions of gel containing albumin, heavy and light chain of IgG before (panel A) and after depletion (panel B). Rectangle: used to highlight different protein patterns in the two groups, indicating a large number of protein spots that emerged in the depleted gel.

    Techniques Used: Electrophoresis, Silver Staining

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    Identification of protein spots unique to depleted umbilical cord serum gels by the Immunoaffinity Albumin and IgG Depletion kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) . Circles: Ten spots unique to the depleted gels were selected for MALDI-TOF/TOF MS identification. Gel image is the same as in Fig 2B. The numbers correspond to the protein numbers in Table 2.
    Figure Legend Snippet: Identification of protein spots unique to depleted umbilical cord serum gels by the Immunoaffinity Albumin and IgG Depletion kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) . Circles: Ten spots unique to the depleted gels were selected for MALDI-TOF/TOF MS identification. Gel image is the same as in Fig 2B. The numbers correspond to the protein numbers in Table 2.

    Techniques Used: Mass Spectrometry

    16) Product Images from "Purification and Functional Characterisation of Rhinocerase, a Novel Serine Protease from the Venom of Bitis gabonica rhinoceros"

    Article Title: Purification and Functional Characterisation of Rhinocerase, a Novel Serine Protease from the Venom of Bitis gabonica rhinoceros

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0009687

    Fibrinogenolytic activity of rhinocerase. Coomassie brilliant blue stained SDS-PAGE gels containing samples taken at different time intervals during the incubation of 100 µg of rhinocerase with 400 µl (5 mg/ml) of plasminogen-free fibrinogen in 0.05M Tris-HCl; 0.1M NaCl, pH 7.8 or 400 µl of human plasma in the presence and absence of 20 mM EDTA. The numbers at the top of each lane represent the time (in minutes) when samples were taken during the digestion. A . The degradation profile of plasminogen-free fibrinogen by rhinocerase. α, β and γ represent the three chains of fibrinogen and A and B represent the degradation products. The position of rhinocerase has been labelled on the image. B . The degradation profile of fibrinogen present in human plasma after removal of albumin and IgG by Proteoprep immunoaffinity albumin and IgG depletion columns. The position of the α chain of fibrinogen is marked with an arrow. Data are representative of three separate experiments.
    Figure Legend Snippet: Fibrinogenolytic activity of rhinocerase. Coomassie brilliant blue stained SDS-PAGE gels containing samples taken at different time intervals during the incubation of 100 µg of rhinocerase with 400 µl (5 mg/ml) of plasminogen-free fibrinogen in 0.05M Tris-HCl; 0.1M NaCl, pH 7.8 or 400 µl of human plasma in the presence and absence of 20 mM EDTA. The numbers at the top of each lane represent the time (in minutes) when samples were taken during the digestion. A . The degradation profile of plasminogen-free fibrinogen by rhinocerase. α, β and γ represent the three chains of fibrinogen and A and B represent the degradation products. The position of rhinocerase has been labelled on the image. B . The degradation profile of fibrinogen present in human plasma after removal of albumin and IgG by Proteoprep immunoaffinity albumin and IgG depletion columns. The position of the α chain of fibrinogen is marked with an arrow. Data are representative of three separate experiments.

    Techniques Used: Activity Assay, Staining, SDS Page, Incubation

    17) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Two Dimensional Electrophoresis Gel image of crude and depleted umbilical cord serum samples . For each gel, 150 μg of protein was separated over pH range 4-7 (24 cm strips) and 12.5% SDS-polyacrylamide gel. The gel was visualized by silver staining. The Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) Kit was used to deplete albumin and IgG in the umbilical cord serum. Removal of these proteins improved the resolution in the albumin and IgG areas on the gel and increased the intensity of low abundance proteins. A) Two Dimension Electrophoresis Gel image of crude umbilical cord serum. B) Two Dimension Electrophoresis Gel image of depleted umbilical cord serum by Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA). Circles: regions of gel containing albumin, heavy and light chain of IgG before (panel A) and after depletion (panel B). Rectangle: used to highlight different protein patterns in the two groups, indicating a large number of protein spots that emerged in the depleted gel.
    Figure Legend Snippet: Two Dimensional Electrophoresis Gel image of crude and depleted umbilical cord serum samples . For each gel, 150 μg of protein was separated over pH range 4-7 (24 cm strips) and 12.5% SDS-polyacrylamide gel. The gel was visualized by silver staining. The Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) Kit was used to deplete albumin and IgG in the umbilical cord serum. Removal of these proteins improved the resolution in the albumin and IgG areas on the gel and increased the intensity of low abundance proteins. A) Two Dimension Electrophoresis Gel image of crude umbilical cord serum. B) Two Dimension Electrophoresis Gel image of depleted umbilical cord serum by Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA). Circles: regions of gel containing albumin, heavy and light chain of IgG before (panel A) and after depletion (panel B). Rectangle: used to highlight different protein patterns in the two groups, indicating a large number of protein spots that emerged in the depleted gel.

    Techniques Used: Electrophoresis, Silver Staining

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    Identification of protein spots unique to depleted umbilical cord serum gels by the Immunoaffinity Albumin and IgG Depletion kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) . Circles: Ten spots unique to the depleted gels were selected for MALDI-TOF/TOF MS identification. Gel image is the same as in Fig 2B. The numbers correspond to the protein numbers in Table 2.
    Figure Legend Snippet: Identification of protein spots unique to depleted umbilical cord serum gels by the Immunoaffinity Albumin and IgG Depletion kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) . Circles: Ten spots unique to the depleted gels were selected for MALDI-TOF/TOF MS identification. Gel image is the same as in Fig 2B. The numbers correspond to the protein numbers in Table 2.

    Techniques Used: Mass Spectrometry

    18) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    19) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Two Dimensional Electrophoresis Gel image of crude and depleted umbilical cord serum samples . For each gel, 150 μg of protein was separated over pH range 4-7 (24 cm strips) and 12.5% SDS-polyacrylamide gel. The gel was visualized by silver staining. The Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) Kit was used to deplete albumin and IgG in the umbilical cord serum. Removal of these proteins improved the resolution in the albumin and IgG areas on the gel and increased the intensity of low abundance proteins. A) Two Dimension Electrophoresis Gel image of crude umbilical cord serum. B) Two Dimension Electrophoresis Gel image of depleted umbilical cord serum by Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA). Circles: regions of gel containing albumin, heavy and light chain of IgG before (panel A) and after depletion (panel B). Rectangle: used to highlight different protein patterns in the two groups, indicating a large number of protein spots that emerged in the depleted gel.
    Figure Legend Snippet: Two Dimensional Electrophoresis Gel image of crude and depleted umbilical cord serum samples . For each gel, 150 μg of protein was separated over pH range 4-7 (24 cm strips) and 12.5% SDS-polyacrylamide gel. The gel was visualized by silver staining. The Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) Kit was used to deplete albumin and IgG in the umbilical cord serum. Removal of these proteins improved the resolution in the albumin and IgG areas on the gel and increased the intensity of low abundance proteins. A) Two Dimension Electrophoresis Gel image of crude umbilical cord serum. B) Two Dimension Electrophoresis Gel image of depleted umbilical cord serum by Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA). Circles: regions of gel containing albumin, heavy and light chain of IgG before (panel A) and after depletion (panel B). Rectangle: used to highlight different protein patterns in the two groups, indicating a large number of protein spots that emerged in the depleted gel.

    Techniques Used: Electrophoresis, Silver Staining

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    Identification of protein spots unique to depleted umbilical cord serum gels by the Immunoaffinity Albumin and IgG Depletion kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) . Circles: Ten spots unique to the depleted gels were selected for MALDI-TOF/TOF MS identification. Gel image is the same as in Fig 2B. The numbers correspond to the protein numbers in Table 2.
    Figure Legend Snippet: Identification of protein spots unique to depleted umbilical cord serum gels by the Immunoaffinity Albumin and IgG Depletion kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) . Circles: Ten spots unique to the depleted gels were selected for MALDI-TOF/TOF MS identification. Gel image is the same as in Fig 2B. The numbers correspond to the protein numbers in Table 2.

    Techniques Used: Mass Spectrometry

    20) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

    21) Product Images from "18O proteomics reveal increased Human Apolipoprotein CIII in Hispanic HIV-1 positive women with HAART that use cocaine"

    Article Title: 18O proteomics reveal increased Human Apolipoprotein CIII in Hispanic HIV-1 positive women with HAART that use cocaine

    Journal: Proteomics. Clinical applications

    doi: 10.1002/prca.201400204

    Depletion gel Plasma from 6 HIV infected and 6 HIV infected- cocaine users were depleted for the most abundant plasma proteins using the ProteoPrep ® Immunoaffinity Albumin IgG Depletion Kit (SIGMA). Plasma proteins (depleted and non-depleted) were separated by SDS-PAGE in a 10% polyacrylamide gel at 40V for 2 hours. A significantly decrease of abundant proteins was observed in depleted plasma samples compared with non-depleted plasma samples.
    Figure Legend Snippet: Depletion gel Plasma from 6 HIV infected and 6 HIV infected- cocaine users were depleted for the most abundant plasma proteins using the ProteoPrep ® Immunoaffinity Albumin IgG Depletion Kit (SIGMA). Plasma proteins (depleted and non-depleted) were separated by SDS-PAGE in a 10% polyacrylamide gel at 40V for 2 hours. A significantly decrease of abundant proteins was observed in depleted plasma samples compared with non-depleted plasma samples.

    Techniques Used: Infection, SDS Page

    22) Product Images from "Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics"

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

    Journal: Proteome Science

    doi: 10.1186/1477-5956-9-24

    Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.
    Figure Legend Snippet: Comparison of depletion efficiency and repeatability of different kits on umbilical cord serum proteins samples by 1D-SDS-PAGE . Ten micrograms of crude, depleted or bound protein were loaded onto each lane and separated by 4-12% polyacrylamide gels. Proteins were visualized by Coomassie Blue staining. For examination of repeatability in umbilical cord serum depletion, the immunoaffinity columns (5185-Agilent and PROTIA-Sigma) were run three consecutive times with re-equilibration between depletion processes. However, for Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA), serum samples were tested in two independent columns since its depletion efficiency was low even with a new column. A) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. B) Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich, Saint Louis, MO, USA) in adult venous serum depletion. C) Multiple Affinity Removal System (5185, Agilent, Santa Clara, CA, USA) in umbilical cord serum depletion. D) Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich, Saint Louis, MO, USA) in umbilical cord serum depletion. Lanes: M: Prestained protein marker (Fermentas; 250, 130; 95, 72, 55, 36, 28, 17); C: Crude serum; D: Depleted serum; B: Bound proteins; D1-3: Depleted umbilical cord serum proteins of the first, second and third depletion process. B1-3: Bound proteins eluted from the column after the first, second and third depletion process. Dp: Depleted Proteins pooled by the three depletion processes. Bp: Bound proteins pooled by the three depletion processes Closed arrows: Protein band indicating albumin; Open arrows: Protein band indicating IgG; * heavy chain, and ** light chain.

    Techniques Used: SDS Page, Staining, Serum Depletion, Marker

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    Two-Dimensional Gel Electrophoresis:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Depletion of albumin and IgG by the PROTIA-Sigma kit improved the quality of 2DE gels in two ways: firstly, depletion of albumin and IgG resulted in improved separation within these regions of the gel and the emergence of several new spots (Figure , circles). ..

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Based on 2DE and MS results, the PROTIA-Sigma Kit dramatically improved 2DE gel quality by increasing low abundance protein intensity, and thus serves as a useful tool in novel biomarker discovery. ..

    Biomarker Assay:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Based on 2DE and MS results, the PROTIA-Sigma Kit dramatically improved 2DE gel quality by increasing low abundance protein intensity, and thus serves as a useful tool in novel biomarker discovery. ..

    Produced:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Therefore, the depleted sera by the PROTBA-Sigma and PROTIA-Sigma kits, as well as the concentrated sera produced by the 5185-Agilent kit were all within the appropriate protein concentration range. .. Both the serum load and the protein yield were highest for the PROTIA-Sigma kit, indicating that it was most suitable for large scale protein preparation.

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Among the three kits, the PROTIA-Sigma kit had the highest load volume1 and produced the most depleted serum (Table ). .. The PROTBA-Sigma Kit had the highest initial yield percentage and concentration of the three kits, likely because albumin and IgG were not completely removed by this kit.

    Mass Spectrometry:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Based on 2DE and MS results, the PROTIA-Sigma Kit dramatically improved 2DE gel quality by increasing low abundance protein intensity, and thus serves as a useful tool in novel biomarker discovery. ..

    Protein Concentration:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Therefore, the depleted sera by the PROTBA-Sigma and PROTIA-Sigma kits, as well as the concentrated sera produced by the 5185-Agilent kit were all within the appropriate protein concentration range. .. Both the serum load and the protein yield were highest for the PROTIA-Sigma kit, indicating that it was most suitable for large scale protein preparation.

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    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Secondly, the depleted serum from the PROTIA-Sigma kit could be used for isoelectric focusing (IEF) directly since the wash solution contained fewer ions. .. Depletion improved 2DE gel quality by two aspects.

    Serum Depletion:

    Article Title: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics
    Article Snippet: .. Conversely, both immunoaffinity based kits, the 5185-Agilent kit and the PROTIA-Sigma kit, had higher efficiency in umbilical cord serum depletion. ..