proteomex ltq mass spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher proteomex ltq mass spectrometer
    Proteomex Ltq Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteomex ltq mass spectrometer/product/Thermo Fisher
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    proteomex ltq mass spectrometer - by Bioz Stars, 2020-05
    85/100 stars

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    Related Articles

    Liquid Chromatography:

    Article Title: Mismatch repair protein MSH2 regulates translesion DNA synthesis following exposure of cells to UV radiation
    Article Snippet: .. The apparent bands were excised, in-gel digested with trypsin and analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) on a ProteomeX-LTQ mass spectrometer (Thermo Fisher Scientific, Waltham, USA) to identify the protein bands. .. Co-immunoprecipitation and western blotting HEK293T cells transfected with peGFP-C3-mREV1 or peGFP-C3-mPolκ and pCMV-Flag-MSH2 were harvested, and the whole-cell lysates were immunoprecipitated with anti-Flag M2 agarose as described previously ( ).

    Mass Spectrometry:

    Article Title: MSMEG_2731, an Uncharacterized Nucleic Acid Binding Protein from Mycobacterium smegmatis, Physically Interacts with RPS1
    Article Snippet: .. The peptides were then analyzed by LC-MS/MS on a ProteomeX-LTQ mass spectrometer (Thermo Fisher Scientific, Waltham, USA). .. Co-IP assay Exponentially-growing cells of M. smegmatis were harvested, resuspended and lysed in lysis buffer B (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 0.1% Triton X-100).

    Article Title: A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement
    Article Snippet: .. Each trypsin-digested and dried sample was reconstituted in 0.1% (v/v) formic acid and analyzed by nanospray-liquid chromatography – tandem mass spectrometry (nESI-LC-MS/MS) performed with a ProteomeX LTQ mass spectrometer (Thermo Fisher, San Jose) as described previously [ ]. .. Database searching Sequences obtained from red rice Illumina transcriptome assembly (OlR) were translated and the open reading frames (ORFs) scanned using Virtual Ribosome software version 1.1 [ ].

    Article Title: Mismatch repair protein MSH2 regulates translesion DNA synthesis following exposure of cells to UV radiation
    Article Snippet: .. The apparent bands were excised, in-gel digested with trypsin and analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) on a ProteomeX-LTQ mass spectrometer (Thermo Fisher Scientific, Waltham, USA) to identify the protein bands. .. Co-immunoprecipitation and western blotting HEK293T cells transfected with peGFP-C3-mREV1 or peGFP-C3-mPolκ and pCMV-Flag-MSH2 were harvested, and the whole-cell lysates were immunoprecipitated with anti-Flag M2 agarose as described previously ( ).

    Article Title: Ionic strength-dependent conformations of a ubiquitin-like small archaeal modifier protein (SAMP2) from Haloferax volcanii
    Article Snippet: .. LC-MS/MS analysis was performed on a ProteomeX-LTQ mass spectrometer (Thermo Fisher), and the data was analyzed by Bioworks Browser (Thermo Fisher). .. NMR experiments and structure calculation NMR spectra for structure calculation were recorded at 293 K on a Bruker DMX500 spectrometer.

    Article Title: Rad9 plays an important role in DNA mismatch repair through physical interaction with MLH1
    Article Snippet: .. The apparent bands were excised, in-gel digested with trypsin, and analyzed by LC-MS/MS on a ProteomeX-LTQ mass spectrometer (Thermo Fisher Scientific, Waltham, USA) to identify the protein bands. .. Co-immunoprecipitation Two vectors, pCDNA3-6HA ( ) and pFLAG-CMV2 (Sigma, St Louis, MO), were used to construct plasmids capable of expressing hMLH1 in human cells.

    Article Title: The Golgi Localization of GOLPH2 (GP73/GOLM1) Is Determined by the Transmembrane and Cytoplamic Sequences
    Article Snippet: .. The GOLPH2-FLAG oligomer band was excised, in-gel digested with trypsin, and analyzed by liquid chromatography-MS/MS on a ProteomeX-LTQ mass spectrometer (Thermo Fisher Scientific) according to previously described methods , The peptide sequences were identified by a data-dependent MS/MS model using a SEQUEST algorithm. .. Yeast two-hybrid A yeast two-hybrid (Y2H) assay for protein-protein interactions was performed following the manufacturer's instructions (Matchmaker™ GAL4 Two-Hybrid System 3, Clonetech).

    Chromatography:

    Article Title: A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement
    Article Snippet: .. Each trypsin-digested and dried sample was reconstituted in 0.1% (v/v) formic acid and analyzed by nanospray-liquid chromatography – tandem mass spectrometry (nESI-LC-MS/MS) performed with a ProteomeX LTQ mass spectrometer (Thermo Fisher, San Jose) as described previously [ ]. .. Database searching Sequences obtained from red rice Illumina transcriptome assembly (OlR) were translated and the open reading frames (ORFs) scanned using Virtual Ribosome software version 1.1 [ ].

    Article Title: The Golgi Localization of GOLPH2 (GP73/GOLM1) Is Determined by the Transmembrane and Cytoplamic Sequences
    Article Snippet: .. The GOLPH2-FLAG oligomer band was excised, in-gel digested with trypsin, and analyzed by liquid chromatography-MS/MS on a ProteomeX-LTQ mass spectrometer (Thermo Fisher Scientific) according to previously described methods , The peptide sequences were identified by a data-dependent MS/MS model using a SEQUEST algorithm. .. Yeast two-hybrid A yeast two-hybrid (Y2H) assay for protein-protein interactions was performed following the manufacturer's instructions (Matchmaker™ GAL4 Two-Hybrid System 3, Clonetech).

    Tandem Mass Spectroscopy:

    Article Title: The Golgi Localization of GOLPH2 (GP73/GOLM1) Is Determined by the Transmembrane and Cytoplamic Sequences
    Article Snippet: .. The GOLPH2-FLAG oligomer band was excised, in-gel digested with trypsin, and analyzed by liquid chromatography-MS/MS on a ProteomeX-LTQ mass spectrometer (Thermo Fisher Scientific) according to previously described methods , The peptide sequences were identified by a data-dependent MS/MS model using a SEQUEST algorithm. .. Yeast two-hybrid A yeast two-hybrid (Y2H) assay for protein-protein interactions was performed following the manufacturer's instructions (Matchmaker™ GAL4 Two-Hybrid System 3, Clonetech).

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    Thermo Fisher proteomex ltq mass spectrometer
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