proteinase k  (Valiant)

 
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  • 86
    Name:
    Proteinase K 30 mAnson U mg
    Description:

    Catalog Number:
    11rist1373
    Price:
    222.7
    Category:
    Life Sciences Biochemicals Proteins and Derivatives Enzymes
    Size:
    100 mg
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    Structured Review

    Valiant proteinase k
    (page width). Schematic illustrating the steps in the CHP microplate assay. (A) After incubation in F-CHP, the sample is rinsed, removing unbound F-CHP. (B) The sample is digested with <t>proteinase</t> K to homogenize it. (C) The fluorescence of the digest is quantified on a 96-well microplate reader.

    https://www.bioz.com/result/proteinase k/product/Valiant
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    proteinase k - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "Microplate Assay for Denatured Collagen using Collagen Hybridizing Peptides"

    Article Title: Microplate Assay for Denatured Collagen using Collagen Hybridizing Peptides

    Journal: bioRxiv

    doi: 10.1101/443242

    (page width). Schematic illustrating the steps in the CHP microplate assay. (A) After incubation in F-CHP, the sample is rinsed, removing unbound F-CHP. (B) The sample is digested with proteinase K to homogenize it. (C) The fluorescence of the digest is quantified on a 96-well microplate reader.
    Figure Legend Snippet: (page width). Schematic illustrating the steps in the CHP microplate assay. (A) After incubation in F-CHP, the sample is rinsed, removing unbound F-CHP. (B) The sample is digested with proteinase K to homogenize it. (C) The fluorescence of the digest is quantified on a 96-well microplate reader.

    Techniques Used: Polyacrylamide Gel Electrophoresis, Incubation, Fluorescence

    2) Product Images from "Microplate Assay for Denatured Collagen using Collagen Hybridizing Peptides"

    Article Title: Microplate Assay for Denatured Collagen using Collagen Hybridizing Peptides

    Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

    doi: 10.1002/jor.24185

    Schematic illustrating the steps in the CHP microplate assay. (A) After incubation in F-CHP, the sample is rinsed, removing unbound F-CHP. (B) The sample is digested with proteinase K to homogenize it. (C) The fluorescence of the digest is quantified on a 96-well microplate reader.
    Figure Legend Snippet: Schematic illustrating the steps in the CHP microplate assay. (A) After incubation in F-CHP, the sample is rinsed, removing unbound F-CHP. (B) The sample is digested with proteinase K to homogenize it. (C) The fluorescence of the digest is quantified on a 96-well microplate reader.

    Techniques Used: Polyacrylamide Gel Electrophoresis, Incubation, Fluorescence

    3) Product Images from "Enzyme-labeled Antigen Method: Histochemical Detection of Antigen-specific Antibody-producing Cells in Tissue Sections of Rats Immunized With Horseradish Peroxidase, Ovalbumin, or Keyhole Limpet Hemocyanin"

    Article Title: Enzyme-labeled Antigen Method: Histochemical Detection of Antigen-specific Antibody-producing Cells in Tissue Sections of Rats Immunized With Horseradish Peroxidase, Ovalbumin, or Keyhole Limpet Hemocyanin

    Journal:

    doi: 10.1369/jhc.2008.952259

    ( A ) Buffered formalin-fixed, paraffin-embedded sections of the axillary lymph nodes of HRP- or KLH-immunized rats reacted with HRP or biotinylated KLH after strong proteinase K pretreatment. A small number of plasma cells producing anti-HRP antibodies
    Figure Legend Snippet: ( A ) Buffered formalin-fixed, paraffin-embedded sections of the axillary lymph nodes of HRP- or KLH-immunized rats reacted with HRP or biotinylated KLH after strong proteinase K pretreatment. A small number of plasma cells producing anti-HRP antibodies

    Techniques Used: Formalin-fixed Paraffin-Embedded

    Related Articles

    Fluorescence:

    Article Title: Microplate Assay for Denatured Collagen using Collagen Hybridizing Peptides
    Article Snippet: Image processing was performed using MATLAB (2016b, MathWorks, Natick, MA). .. CHP Microplate AssayTo enable the detection of F-CHP fluorescence using a microplate reader, samples were homogenized in 500 μL of a 1 mg/mL proteinase K (Cat No. 193981, MP Biomedicals, LLC, Santa Ana, CA) solution in deionized water for three hours at 60°C. .. After homogenization, the fluorescence of 200 μL duplicates was measured at excitation and emission wavelengths of 485 and 525 nm, respectively on a microplate reader (SpectraMax Gemini XPS, Molecular Devices, Sunnyvale, CA).

    Article Title: Microplate Assay for Denatured Collagen using Collagen Hybridizing Peptides
    Article Snippet: Image processing was performed using MATLAB (2016b, MathWorks, Natick, MA). .. To enable the detection of F-CHP fluorescence using a microplate reader, samples were homogenized in 500 μL of a 1 mg/mL proteinase K (Cat No. 193981, MP Biomedicals, LLC, Santa Ana, CA) solution in deionized water for three hours at 60°C. .. After homogenization, the fluorescence of 200 μL duplicates was measured at excitation and emission wavelengths of 485 and 525 nm, respectively on a microplate reader (SpectraMax Gemini XPS, Molecular Devices, Sunnyvale, CA).

    Incubation:

    Article Title: Enzyme-labeled Antigen Method: Histochemical Detection of Antigen-specific Antibody-producing Cells in Tissue Sections of Rats Immunized With Horseradish Peroxidase, Ovalbumin, or Keyhole Limpet Hemocyanin
    Article Snippet: Overnight incubation with the antigen solutions was tested when negative for 1-hr incubation. .. After a brief dip in running water and endogenous peroxidase blockage, the frozen sections were treated with Tris-buffered saline containing 5 μg/ml proteinase K for 15 min at room temperature, rinsed in PBS, and incubated with a cocktail of HRP-conjugated goat anti-rat IgG, IgA, and IgM polyvalent antibodies (MP Biomedicals; Solon, OH), diluted at 1:1000, for 1 hr at room temperature. ..

    Article Title: Dynamic in vivo mutations within the ica operon during persistence of Staphylococcus aureus in the airways of cystic fibrosis patients
    Article Snippet: .. Therefore, microtiter plates that were incubated for 24 h at 37°C were treated either with 100 μl sodium-metaperiodate in water (40 mM; AppliChem, Darmstadt, Germany), DNase I in 150 mM NaCl/1mM CaCl2 (100 μg/ml; Roche, Mannheim, Germany) or proteinase K in 10 mM Tris-HCl (pH 7.5) (100 μg/ml; MP Biomedicals, Santa Ana, California, USA) for 3 h at 37°C. .. After three washing steps with PBS, biofilms were stained with crystal violet, washed again with PBS and solubilized in ethanol-acetone as described above.

    Article Title: An improved definition of the RNA-binding specificity of SECIS-binding protein 2, an essential component of the selenocysteine incorporation machinery
    Article Snippet: For RNP complexes, 0.1 nmol of treated RNAs was incubated with 0.01 nmol of purified GST/C-SBP2 for 30 min at 4°C, in 20 μl of buffer D, in the presence of 2 μg of a yeast tRNA mixture (Roche). .. The mixture was then incubated with 15 μl of glutathione-sepharose beads (Amersham) equilibrated in buffer D. After extensive washing with buffer D, the selected RNAs were released by a 30-min incubation at 37°C, with 20 μg of proteinase K in buffer D. They were extracted with a phenol–chloroform mixture, ethanol precipitated, dissolved in sterile water, hybridized with 50 pmol of SELEX-3′ primer, ethanol precipitated, and finally reverse-transcribed with 25 U of AMV Reverse transcriptase (Q.Biogene) for 30 min at 42°C. .. Next, 30 cycles of PCR amplification were performed in the presence of primers SELEX-5′ and SELEX-3′ (50 pmol each).

    Purification:

    Article Title: Feline Foamy Virus Infection: Characterization of Experimental Infection and Prevalence of Natural Infection in Domestic Cats with and without Chronic Kidney Disease
    Article Snippet: Real-time quantitative PCR (qPCR) was performed in duplicate to triplicate on purified FFV DNA as described [ , ]. .. Tissue DNA was purified with the DNeasy Blood and Tissue Kit after homogenizing in Buffer ATL and Proteinase K using the FastPrep®-24 Instrument (MP Biomedicals, Santa Ana, CA, USA). .. Saliva and plasma RNA was purified using the QIAamp Viral RNA Mini Kit (QIAGEN).

    other:

    Article Title: The Prodomain of the Bordetella Two-Partner Secretion Pathway Protein FhaB Remains Intracellular yet Affects the Conformation of the Mature C-terminal Domain
    Article Snippet: 200 μL of mock reaction buffer (HEPES, pH 7.4), 1.0 μg/mL proteinase-K (Sigma-Aldrich) in HEPES, or 1.0 μg/mL proteinase-K with 1 mM PMSF (MP Biomedicals) in HEPES were added to each of the three samples per strain.

    Picogreen Assay:

    Article Title: Accumulation of Exogenous Activated TGF-β in the Superficial Zone of Articular Cartilage
    Article Snippet: Conditioned media were frozen and subsequently tested for the mass of active and latent TGF- β 1 released from each explant and expressed as the concentration based on the volume of the explant (approximated from mass measurements). .. At the end of the culture period, all live cartilage and synovial tissue explants were digested in 0.5 mg/mL Proteinase-K (MP Biomedicals) in 50 mM Tris-buffered saline containing 1 mM ethylenediaminetetraacetic acid, 1 mM iodoacetamide, and 10 mg/mL pepstatin A, at 56°C for 16 h. The DNA content of digested samples was quantified with the PicoGreen assay (Invitrogen), as described previously for cartilage , with bacteriophage λ DNA standards. ..

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  • 94
    Valiant proteinase k
    Proteinase K, supplied by Valiant, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k/product/Valiant
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    proteinase k - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

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