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Agilent technologies proteinase k
Proteinase K, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proteinase k/product/Agilent technologies
Average 97 stars, based on 286 article reviews
Price from $9.99 to $1999.99
proteinase k - by Bioz Stars, 2020-01
97/100 stars

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TUNEL Assay:

Article Title: The Combination of Human Urinary Kallidinogenase and Mild Hypothermia Protects Adult Rats Against Hypoxic-Ischemic Encephalopathy-Induced Injury by Promoting Angiogenesis and Regeneration
Article Snippet: Paragraph title: Terminal Deoxynucleotidyl Transferase-Mediated dUTP Nick End-Labeling (TUNEL) Staining ... Briefly, we prepared 5 μm thick paraffin sections that were then immersed in dimethylbenzene I and II (Sigma Aldrich, 296333, USA) for 15 min and then sequentially dewaxed at 100%, 85%, 75% ethanol (Sigma Aldrich, 49836, USA) and distilled water for 5 min. Next, we incubated the sections with diluted protease K (DAKO, Cat. #S3020, USA) at 37°C for 30 min and with Permeabilization Buffer (Aviva, OOMB00004, USA) for 20 min at room temperature.

Immunohistochemistry:

Article Title: Pivotal Preclinical Trial of the Spheroid Reservoir Bioartificial Liver
Article Snippet: Immunohistochemical sections were treated with 3% hydrogen peroxide in ethanol for five minutes to inactivate endogenous peroxidase. .. Sections were pretreated with protease K (DAKO, Carpentaria, California), and endogenous pig immunoglobulins were blocked using porcine block M (Biocare Medical, Concord, California).

Article Title: Middle ear mucosal regeneration by tissue-engineered cell sheet transplantation
Article Snippet: .. For immunohistochemistry, de-paraffinized sections were washed with phosphate-buffered saline and digested with protease K (DakoCytomation, Glostrup, Denmark). .. Sections were then treated with each of the following antibodies according to the manufacturers’ suggested protocols: mouse monoclonal anti-pancytokeratin (1:20 dilution, AE1/AE3, Abcam, Cambridge, UK), mouse monoclonal anti-E-cadherin (1:100 dilution, NCH-38, DakoCytomation) and anti-p63 (1:100 dilution, V9, DakoCytomation).

Article Title: COX-2 and Prostaglandin EP3/EP4 Signaling Regulate the Tumor Stromal Proangiogenic Microenvironment via CXCL12-CXCR4 Chemokine Systems
Article Snippet: Paragraph title: Immunohistochemistry ... For immunostaining, antigen unmasking was performed with Protease-K (DakoCytomation, Glostrup, Denmark) for three minutes, endogenous peroxidase activity was blocked by incubation in 3% hydrogen peroxide/phosphate buffer for five minutes, and nonspecific antibody binding was blocked by incubation with 1% diluted normal horse serum for 30 minutes.

In Situ:

Article Title: The Combination of Human Urinary Kallidinogenase and Mild Hypothermia Protects Adult Rats Against Hypoxic-Ischemic Encephalopathy-Induced Injury by Promoting Angiogenesis and Regeneration
Article Snippet: TUNEL staining was conducted using the in situ Cell Death Detection Kit (Roche, 11684795910, Germany) following the manufacturer’s instructions. .. Briefly, we prepared 5 μm thick paraffin sections that were then immersed in dimethylbenzene I and II (Sigma Aldrich, 296333, USA) for 15 min and then sequentially dewaxed at 100%, 85%, 75% ethanol (Sigma Aldrich, 49836, USA) and distilled water for 5 min. Next, we incubated the sections with diluted protease K (DAKO, Cat. #S3020, USA) at 37°C for 30 min and with Permeabilization Buffer (Aviva, OOMB00004, USA) for 20 min at room temperature.

Fluorescence:

Article Title: The Combination of Human Urinary Kallidinogenase and Mild Hypothermia Protects Adult Rats Against Hypoxic-Ischemic Encephalopathy-Induced Injury by Promoting Angiogenesis and Regeneration
Article Snippet: Briefly, we prepared 5 μm thick paraffin sections that were then immersed in dimethylbenzene I and II (Sigma Aldrich, 296333, USA) for 15 min and then sequentially dewaxed at 100%, 85%, 75% ethanol (Sigma Aldrich, 49836, USA) and distilled water for 5 min. Next, we incubated the sections with diluted protease K (DAKO, Cat. #S3020, USA) at 37°C for 30 min and with Permeabilization Buffer (Aviva, OOMB00004, USA) for 20 min at room temperature. .. The prepared sections were photographed with a NIKON ECLIPSE TI-SR inverted fluorescence microscope (NIKON, Japan) with a UV excitation wavelength 330–380 nm, emission wavelength 420 nm (for DAPI); and FITC excitation 465–495 nm, emission 515–555 nm (for TUNEL).

Software:

Article Title: The Combination of Human Urinary Kallidinogenase and Mild Hypothermia Protects Adult Rats Against Hypoxic-Ischemic Encephalopathy-Induced Injury by Promoting Angiogenesis and Regeneration
Article Snippet: Briefly, we prepared 5 μm thick paraffin sections that were then immersed in dimethylbenzene I and II (Sigma Aldrich, 296333, USA) for 15 min and then sequentially dewaxed at 100%, 85%, 75% ethanol (Sigma Aldrich, 49836, USA) and distilled water for 5 min. Next, we incubated the sections with diluted protease K (DAKO, Cat. #S3020, USA) at 37°C for 30 min and with Permeabilization Buffer (Aviva, OOMB00004, USA) for 20 min at room temperature. .. The images were quantified using Image-Pro Plus 6.0 software (Media Cybernetics Inc., Rockville, MD, USA).

Microscopy:

Article Title: The Combination of Human Urinary Kallidinogenase and Mild Hypothermia Protects Adult Rats Against Hypoxic-Ischemic Encephalopathy-Induced Injury by Promoting Angiogenesis and Regeneration
Article Snippet: Briefly, we prepared 5 μm thick paraffin sections that were then immersed in dimethylbenzene I and II (Sigma Aldrich, 296333, USA) for 15 min and then sequentially dewaxed at 100%, 85%, 75% ethanol (Sigma Aldrich, 49836, USA) and distilled water for 5 min. Next, we incubated the sections with diluted protease K (DAKO, Cat. #S3020, USA) at 37°C for 30 min and with Permeabilization Buffer (Aviva, OOMB00004, USA) for 20 min at room temperature. .. The prepared sections were photographed with a NIKON ECLIPSE TI-SR inverted fluorescence microscope (NIKON, Japan) with a UV excitation wavelength 330–380 nm, emission wavelength 420 nm (for DAPI); and FITC excitation 465–495 nm, emission 515–555 nm (for TUNEL).

Labeling:

Article Title: Pivotal Preclinical Trial of the Spheroid Reservoir Bioartificial Liver
Article Snippet: Sections were pretreated with protease K (DAKO, Carpentaria, California), and endogenous pig immunoglobulins were blocked using porcine block M (Biocare Medical, Concord, California). .. Labeling of primary antibody was carried out by using the Biocare Medical kit antibody and diaminobenzidine.

Mouse Assay:

Article Title: COX-2 and Prostaglandin EP3/EP4 Signaling Regulate the Tumor Stromal Proangiogenic Microenvironment via CXCL12-CXCR4 Chemokine Systems
Article Snippet: For immunostaining, antigen unmasking was performed with Protease-K (DakoCytomation, Glostrup, Denmark) for three minutes, endogenous peroxidase activity was blocked by incubation in 3% hydrogen peroxide/phosphate buffer for five minutes, and nonspecific antibody binding was blocked by incubation with 1% diluted normal horse serum for 30 minutes. .. Immune complexes of primary antibodies raised in mice were detected with the mouse-on-mouse (M.O.M.) kit (Histofine Mouse Stain Kit, Nichirei Biosciences Inc., Tokyo, Japan), according to the manufacturer's instructions.

Incubation:

Article Title: Pivotal Preclinical Trial of the Spheroid Reservoir Bioartificial Liver
Article Snippet: Sections were pretreated with protease K (DAKO, Carpentaria, California), and endogenous pig immunoglobulins were blocked using porcine block M (Biocare Medical, Concord, California). .. Sections were incubated with rat anti pig primary antibody (Ki67) for 60 minutes using 1–400 dilution.

Article Title: The Combination of Human Urinary Kallidinogenase and Mild Hypothermia Protects Adult Rats Against Hypoxic-Ischemic Encephalopathy-Induced Injury by Promoting Angiogenesis and Regeneration
Article Snippet: .. Briefly, we prepared 5 μm thick paraffin sections that were then immersed in dimethylbenzene I and II (Sigma Aldrich, 296333, USA) for 15 min and then sequentially dewaxed at 100%, 85%, 75% ethanol (Sigma Aldrich, 49836, USA) and distilled water for 5 min. Next, we incubated the sections with diluted protease K (DAKO, Cat. #S3020, USA) at 37°C for 30 min and with Permeabilization Buffer (Aviva, OOMB00004, USA) for 20 min at room temperature. .. Finally, we incubated the sections with a mixed TUNEL working solution (TdT: dUTP = 2:29) at 37°C for 2 h, stained the nuclei with DAPI (Sigma Aldrich, D9542, USA) at room temperature for 10 min, and sealed the sections with the anti-fluorescence quenching sealing agent Fluoroshield™ (Sigma Aldrich, F6182, USA).

Article Title: COX-2 and Prostaglandin EP3/EP4 Signaling Regulate the Tumor Stromal Proangiogenic Microenvironment via CXCL12-CXCR4 Chemokine Systems
Article Snippet: .. For immunostaining, antigen unmasking was performed with Protease-K (DakoCytomation, Glostrup, Denmark) for three minutes, endogenous peroxidase activity was blocked by incubation in 3% hydrogen peroxide/phosphate buffer for five minutes, and nonspecific antibody binding was blocked by incubation with 1% diluted normal horse serum for 30 minutes. .. Sections were then incubated at 4°C overnight with the following antibodies: rat anti-mouse CD31 monoclonal IgG2a antibody (Abcam plc, CB, UK), to identify endothelial cells of the microvessels; mouse anti-mouse SDF-1 monoclonal IgG1 antibody (P-159X: Santa Cruz Biotechnology, Inc., Santa Cruz, CA), to detect SDF-1α (CXCL12); rat anti-mouse CXCR4 monoclonal IgG2b antibody (2B11: Japan BD Biosciences, Tokyo, Japan), to detect CXCR4; rabbit anti-mouse S100A4 polyclonal IgG antibody (Abcam plc, CB, UK), to identify fibroblasts in the stromal tissue; rat anti-mouse F4/80 monoclonal IgG2a antibody (BM8: Santa Cruz Biotechnology Inc., Santa Cruz, CA) or goat anti-mouse F4/80 polyclonal IgG antibody (A-19: Santa Cruz Biotechnology Inc., Santa Cruz, CA) to identify monocytes; goat anti-mouse CD3ε polyclonal IgG antibody to identify lymphocytes (M-20: Santa Cruz Biotechnology Inc., Santa Cruz, CA), and rabbit anti-mouse α-smooth muscle actin (α-SMA) monoclonal IgG antibody (E184: Abcam plc, CB, UK) or mouse anti-mouse α-SMA monoclonal IgG2a antibody (1A4: Sigma-Aldrich Inc., St. Louis, MO) to identify myofibroblasts.

Formalin-fixed Paraffin-Embedded:

Article Title: Chronic Inflammatory Demyelinating Polyneuropathy With Concurrent Membranous Nephropathy: An Anti-paranode and Podocyte Protein Antibody Study and Literature Survey
Article Snippet: .. Immunofluorescence staining was performed using formalin-fixed paraffin-embedded sections that were prepared by protease K (Dako, Copenhagen, Denmark) for 60 min. Fluorescein isothiocyanate-labeled rabbit anti-human IgG, IgA, IgM, C1q, C3, fibrinogen, kappa- and lambda-light chains (Dako) were used. .. Additionally, mouse monoclonal anti-human IgG1, IgG2, IgG4 (Chemicon, Temecula, CA, USA), and IgG3 (Thermo Fisher Scientific) antibodies, and rabbit polyclonal anti-PLA2R antibodies (Abcam) were used followed by Alexa 546 goat anti-mouse IgG (H+L) and Alexa 488 goat anti-rabbit IgG (H+L) (Thermo Fisher Scientific).

Activity Assay:

Article Title: COX-2 and Prostaglandin EP3/EP4 Signaling Regulate the Tumor Stromal Proangiogenic Microenvironment via CXCL12-CXCR4 Chemokine Systems
Article Snippet: .. For immunostaining, antigen unmasking was performed with Protease-K (DakoCytomation, Glostrup, Denmark) for three minutes, endogenous peroxidase activity was blocked by incubation in 3% hydrogen peroxide/phosphate buffer for five minutes, and nonspecific antibody binding was blocked by incubation with 1% diluted normal horse serum for 30 minutes. .. Sections were then incubated at 4°C overnight with the following antibodies: rat anti-mouse CD31 monoclonal IgG2a antibody (Abcam plc, CB, UK), to identify endothelial cells of the microvessels; mouse anti-mouse SDF-1 monoclonal IgG1 antibody (P-159X: Santa Cruz Biotechnology, Inc., Santa Cruz, CA), to detect SDF-1α (CXCL12); rat anti-mouse CXCR4 monoclonal IgG2b antibody (2B11: Japan BD Biosciences, Tokyo, Japan), to detect CXCR4; rabbit anti-mouse S100A4 polyclonal IgG antibody (Abcam plc, CB, UK), to identify fibroblasts in the stromal tissue; rat anti-mouse F4/80 monoclonal IgG2a antibody (BM8: Santa Cruz Biotechnology Inc., Santa Cruz, CA) or goat anti-mouse F4/80 polyclonal IgG antibody (A-19: Santa Cruz Biotechnology Inc., Santa Cruz, CA) to identify monocytes; goat anti-mouse CD3ε polyclonal IgG antibody to identify lymphocytes (M-20: Santa Cruz Biotechnology Inc., Santa Cruz, CA), and rabbit anti-mouse α-smooth muscle actin (α-SMA) monoclonal IgG antibody (E184: Abcam plc, CB, UK) or mouse anti-mouse α-SMA monoclonal IgG2a antibody (1A4: Sigma-Aldrich Inc., St. Louis, MO) to identify myofibroblasts.

Blocking Assay:

Article Title: Pivotal Preclinical Trial of the Spheroid Reservoir Bioartificial Liver
Article Snippet: .. Sections were pretreated with protease K (DAKO, Carpentaria, California), and endogenous pig immunoglobulins were blocked using porcine block M (Biocare Medical, Concord, California). .. Sections were incubated with rat anti pig primary antibody (Ki67) for 60 minutes using 1–400 dilution.

Inverted Microscopy:

Article Title: Pivotal Preclinical Trial of the Spheroid Reservoir Bioartificial Liver
Article Snippet: Sections were pretreated with protease K (DAKO, Carpentaria, California), and endogenous pig immunoglobulins were blocked using porcine block M (Biocare Medical, Concord, California). .. Sections were then counter stained using modified Schmitz hematoxylin and visualized using Axiovert 135 inverted microscope (Axioscope, Carl Zeiss, Inc., Thornwood, NJ).

End Labeling:

Article Title: The Combination of Human Urinary Kallidinogenase and Mild Hypothermia Protects Adult Rats Against Hypoxic-Ischemic Encephalopathy-Induced Injury by Promoting Angiogenesis and Regeneration
Article Snippet: Paragraph title: Terminal Deoxynucleotidyl Transferase-Mediated dUTP Nick End-Labeling (TUNEL) Staining ... Briefly, we prepared 5 μm thick paraffin sections that were then immersed in dimethylbenzene I and II (Sigma Aldrich, 296333, USA) for 15 min and then sequentially dewaxed at 100%, 85%, 75% ethanol (Sigma Aldrich, 49836, USA) and distilled water for 5 min. Next, we incubated the sections with diluted protease K (DAKO, Cat. #S3020, USA) at 37°C for 30 min and with Permeabilization Buffer (Aviva, OOMB00004, USA) for 20 min at room temperature.

Modification:

Article Title: Pivotal Preclinical Trial of the Spheroid Reservoir Bioartificial Liver
Article Snippet: Sections were pretreated with protease K (DAKO, Carpentaria, California), and endogenous pig immunoglobulins were blocked using porcine block M (Biocare Medical, Concord, California). .. Sections were then counter stained using modified Schmitz hematoxylin and visualized using Axiovert 135 inverted microscope (Axioscope, Carl Zeiss, Inc., Thornwood, NJ).

Planar Chromatography:

Article Title: COX-2 and Prostaglandin EP3/EP4 Signaling Regulate the Tumor Stromal Proangiogenic Microenvironment via CXCL12-CXCR4 Chemokine Systems
Article Snippet: For immunostaining, antigen unmasking was performed with Protease-K (DakoCytomation, Glostrup, Denmark) for three minutes, endogenous peroxidase activity was blocked by incubation in 3% hydrogen peroxide/phosphate buffer for five minutes, and nonspecific antibody binding was blocked by incubation with 1% diluted normal horse serum for 30 minutes. .. Sections were then incubated at 4°C overnight with the following antibodies: rat anti-mouse CD31 monoclonal IgG2a antibody (Abcam plc, CB, UK), to identify endothelial cells of the microvessels; mouse anti-mouse SDF-1 monoclonal IgG1 antibody (P-159X: Santa Cruz Biotechnology, Inc., Santa Cruz, CA), to detect SDF-1α (CXCL12); rat anti-mouse CXCR4 monoclonal IgG2b antibody (2B11: Japan BD Biosciences, Tokyo, Japan), to detect CXCR4; rabbit anti-mouse S100A4 polyclonal IgG antibody (Abcam plc, CB, UK), to identify fibroblasts in the stromal tissue; rat anti-mouse F4/80 monoclonal IgG2a antibody (BM8: Santa Cruz Biotechnology Inc., Santa Cruz, CA) or goat anti-mouse F4/80 polyclonal IgG antibody (A-19: Santa Cruz Biotechnology Inc., Santa Cruz, CA) to identify monocytes; goat anti-mouse CD3ε polyclonal IgG antibody to identify lymphocytes (M-20: Santa Cruz Biotechnology Inc., Santa Cruz, CA), and rabbit anti-mouse α-smooth muscle actin (α-SMA) monoclonal IgG antibody (E184: Abcam plc, CB, UK) or mouse anti-mouse α-SMA monoclonal IgG2a antibody (1A4: Sigma-Aldrich Inc., St. Louis, MO) to identify myofibroblasts.

Immunostaining:

Article Title: COX-2 and Prostaglandin EP3/EP4 Signaling Regulate the Tumor Stromal Proangiogenic Microenvironment via CXCL12-CXCR4 Chemokine Systems
Article Snippet: .. For immunostaining, antigen unmasking was performed with Protease-K (DakoCytomation, Glostrup, Denmark) for three minutes, endogenous peroxidase activity was blocked by incubation in 3% hydrogen peroxide/phosphate buffer for five minutes, and nonspecific antibody binding was blocked by incubation with 1% diluted normal horse serum for 30 minutes. .. Sections were then incubated at 4°C overnight with the following antibodies: rat anti-mouse CD31 monoclonal IgG2a antibody (Abcam plc, CB, UK), to identify endothelial cells of the microvessels; mouse anti-mouse SDF-1 monoclonal IgG1 antibody (P-159X: Santa Cruz Biotechnology, Inc., Santa Cruz, CA), to detect SDF-1α (CXCL12); rat anti-mouse CXCR4 monoclonal IgG2b antibody (2B11: Japan BD Biosciences, Tokyo, Japan), to detect CXCR4; rabbit anti-mouse S100A4 polyclonal IgG antibody (Abcam plc, CB, UK), to identify fibroblasts in the stromal tissue; rat anti-mouse F4/80 monoclonal IgG2a antibody (BM8: Santa Cruz Biotechnology Inc., Santa Cruz, CA) or goat anti-mouse F4/80 polyclonal IgG antibody (A-19: Santa Cruz Biotechnology Inc., Santa Cruz, CA) to identify monocytes; goat anti-mouse CD3ε polyclonal IgG antibody to identify lymphocytes (M-20: Santa Cruz Biotechnology Inc., Santa Cruz, CA), and rabbit anti-mouse α-smooth muscle actin (α-SMA) monoclonal IgG antibody (E184: Abcam plc, CB, UK) or mouse anti-mouse α-SMA monoclonal IgG2a antibody (1A4: Sigma-Aldrich Inc., St. Louis, MO) to identify myofibroblasts.

Binding Assay:

Article Title: COX-2 and Prostaglandin EP3/EP4 Signaling Regulate the Tumor Stromal Proangiogenic Microenvironment via CXCL12-CXCR4 Chemokine Systems
Article Snippet: .. For immunostaining, antigen unmasking was performed with Protease-K (DakoCytomation, Glostrup, Denmark) for three minutes, endogenous peroxidase activity was blocked by incubation in 3% hydrogen peroxide/phosphate buffer for five minutes, and nonspecific antibody binding was blocked by incubation with 1% diluted normal horse serum for 30 minutes. .. Sections were then incubated at 4°C overnight with the following antibodies: rat anti-mouse CD31 monoclonal IgG2a antibody (Abcam plc, CB, UK), to identify endothelial cells of the microvessels; mouse anti-mouse SDF-1 monoclonal IgG1 antibody (P-159X: Santa Cruz Biotechnology, Inc., Santa Cruz, CA), to detect SDF-1α (CXCL12); rat anti-mouse CXCR4 monoclonal IgG2b antibody (2B11: Japan BD Biosciences, Tokyo, Japan), to detect CXCR4; rabbit anti-mouse S100A4 polyclonal IgG antibody (Abcam plc, CB, UK), to identify fibroblasts in the stromal tissue; rat anti-mouse F4/80 monoclonal IgG2a antibody (BM8: Santa Cruz Biotechnology Inc., Santa Cruz, CA) or goat anti-mouse F4/80 polyclonal IgG antibody (A-19: Santa Cruz Biotechnology Inc., Santa Cruz, CA) to identify monocytes; goat anti-mouse CD3ε polyclonal IgG antibody to identify lymphocytes (M-20: Santa Cruz Biotechnology Inc., Santa Cruz, CA), and rabbit anti-mouse α-smooth muscle actin (α-SMA) monoclonal IgG antibody (E184: Abcam plc, CB, UK) or mouse anti-mouse α-SMA monoclonal IgG2a antibody (1A4: Sigma-Aldrich Inc., St. Louis, MO) to identify myofibroblasts.

Immunofluorescence:

Article Title: Chronic Inflammatory Demyelinating Polyneuropathy With Concurrent Membranous Nephropathy: An Anti-paranode and Podocyte Protein Antibody Study and Literature Survey
Article Snippet: .. Immunofluorescence staining was performed using formalin-fixed paraffin-embedded sections that were prepared by protease K (Dako, Copenhagen, Denmark) for 60 min. Fluorescein isothiocyanate-labeled rabbit anti-human IgG, IgA, IgM, C1q, C3, fibrinogen, kappa- and lambda-light chains (Dako) were used. .. Additionally, mouse monoclonal anti-human IgG1, IgG2, IgG4 (Chemicon, Temecula, CA, USA), and IgG3 (Thermo Fisher Scientific) antibodies, and rabbit polyclonal anti-PLA2R antibodies (Abcam) were used followed by Alexa 546 goat anti-mouse IgG (H+L) and Alexa 488 goat anti-rabbit IgG (H+L) (Thermo Fisher Scientific).

Plasmid Preparation:

Article Title: COX-2 and Prostaglandin EP3/EP4 Signaling Regulate the Tumor Stromal Proangiogenic Microenvironment via CXCL12-CXCR4 Chemokine Systems
Article Snippet: For immunostaining, antigen unmasking was performed with Protease-K (DakoCytomation, Glostrup, Denmark) for three minutes, endogenous peroxidase activity was blocked by incubation in 3% hydrogen peroxide/phosphate buffer for five minutes, and nonspecific antibody binding was blocked by incubation with 1% diluted normal horse serum for 30 minutes. .. For immunostaining, antigen unmasking was performed with Protease-K (DakoCytomation, Glostrup, Denmark) for three minutes, endogenous peroxidase activity was blocked by incubation in 3% hydrogen peroxide/phosphate buffer for five minutes, and nonspecific antibody binding was blocked by incubation with 1% diluted normal horse serum for 30 minutes.

Staining:

Article Title: Pivotal Preclinical Trial of the Spheroid Reservoir Bioartificial Liver
Article Snippet: Liver tissue was formalin fixed and paraffin embedded for permanent staining by hematoxylin and eosin (H & E), as well as immunohistochemical staining with an anti-Ki67 antibody (#M7240, lot#20001030, ). .. Sections were pretreated with protease K (DAKO, Carpentaria, California), and endogenous pig immunoglobulins were blocked using porcine block M (Biocare Medical, Concord, California).

Article Title: The Combination of Human Urinary Kallidinogenase and Mild Hypothermia Protects Adult Rats Against Hypoxic-Ischemic Encephalopathy-Induced Injury by Promoting Angiogenesis and Regeneration
Article Snippet: Paragraph title: Terminal Deoxynucleotidyl Transferase-Mediated dUTP Nick End-Labeling (TUNEL) Staining ... Briefly, we prepared 5 μm thick paraffin sections that were then immersed in dimethylbenzene I and II (Sigma Aldrich, 296333, USA) for 15 min and then sequentially dewaxed at 100%, 85%, 75% ethanol (Sigma Aldrich, 49836, USA) and distilled water for 5 min. Next, we incubated the sections with diluted protease K (DAKO, Cat. #S3020, USA) at 37°C for 30 min and with Permeabilization Buffer (Aviva, OOMB00004, USA) for 20 min at room temperature.

Article Title: Chronic Inflammatory Demyelinating Polyneuropathy With Concurrent Membranous Nephropathy: An Anti-paranode and Podocyte Protein Antibody Study and Literature Survey
Article Snippet: .. Immunofluorescence staining was performed using formalin-fixed paraffin-embedded sections that were prepared by protease K (Dako, Copenhagen, Denmark) for 60 min. Fluorescein isothiocyanate-labeled rabbit anti-human IgG, IgA, IgM, C1q, C3, fibrinogen, kappa- and lambda-light chains (Dako) were used. .. Additionally, mouse monoclonal anti-human IgG1, IgG2, IgG4 (Chemicon, Temecula, CA, USA), and IgG3 (Thermo Fisher Scientific) antibodies, and rabbit polyclonal anti-PLA2R antibodies (Abcam) were used followed by Alexa 546 goat anti-mouse IgG (H+L) and Alexa 488 goat anti-rabbit IgG (H+L) (Thermo Fisher Scientific).

Article Title: Middle ear mucosal regeneration by tissue-engineered cell sheet transplantation
Article Snippet: Hematoxylin and eosin (HE) staining was performed by conventional methods. .. For immunohistochemistry, de-paraffinized sections were washed with phosphate-buffered saline and digested with protease K (DakoCytomation, Glostrup, Denmark).

Article Title: COX-2 and Prostaglandin EP3/EP4 Signaling Regulate the Tumor Stromal Proangiogenic Microenvironment via CXCL12-CXCR4 Chemokine Systems
Article Snippet: After deparaffinization with xylene and hydration with a degraded series of ethanol solution, the sections were subjected to either H & E staining or immunostaining. .. For immunostaining, antigen unmasking was performed with Protease-K (DakoCytomation, Glostrup, Denmark) for three minutes, endogenous peroxidase activity was blocked by incubation in 3% hydrogen peroxide/phosphate buffer for five minutes, and nonspecific antibody binding was blocked by incubation with 1% diluted normal horse serum for 30 minutes.

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    Agilent technologies proteinase k
    Proteinase K, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k/product/Agilent technologies
    Average 97 stars, based on 286 article reviews
    Price from $9.99 to $1999.99
    proteinase k - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

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