protein concentrator pes  (Thermo Fisher)


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    Structured Review

    Thermo Fisher protein concentrator pes
    Protein Concentrator Pes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein concentrator pes/product/Thermo Fisher
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    protein concentrator pes - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Canavanine-Induced Decrease in Nitric Oxide Synthesis Alters Activity of Antioxidant System but Does Not Impact S-Nitrosoglutathione Catabolism in Tomato Roots
    Article Snippet: .. After centrifugation, supernatant was concentrated with Pierce™ Protein Concentrator PES, 3K MWCO (Thermo Scientific™ ) to obtain 2 ml of protein extract. ..

    Sonication:

    Article Title: PAXX Participates in Base Excision Repair via Interacting with Pol β and Contributes to TMZ Resistance in Glioma Cells
    Article Snippet: Lysate was then sonicated by using Branson Sonifier 15%, 10× 10 s to shear DNA to lower viscosity. .. Protein was concentrated by using Pierce™ Protein Concentrator PES, 3 K MWCO (ThermoFisher, Cat. No. 88514) and 30 K (ThermoFisher, Cat. No. 88529) for PAXX and PARP1 respectively in dialyzing buffer (50 mM Tris pH 7.9, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, and 10% glycerol) to lower the salt concentration.

    Protease Inhibitor:

    Article Title: Canavanine-Induced Decrease in Nitric Oxide Synthesis Alters Activity of Antioxidant System but Does Not Impact S-Nitrosoglutathione Catabolism in Tomato Roots
    Article Snippet: Protein Extraction and Purification Roots (2 g) were homogenized in liquid nitrogen, and then, proteins were extracted by adding 5 ml of 0.1-M Tris-HCl buffer (pH 7.0) with 1% (w/v) Triton X-100, 2% (w/v) glycerol, 2-mM DTT, 0.15-M NaCl, 1% (v/v) protease inhibitor cocktail (Sigma-Aldrich), and 5% (w/v) PVPP. .. After centrifugation, supernatant was concentrated with Pierce™ Protein Concentrator PES, 3K MWCO (Thermo Scientific™ ) to obtain 2 ml of protein extract.

    Article Title: PAXX Participates in Base Excision Repair via Interacting with Pol β and Contributes to TMZ Resistance in Glioma Cells
    Article Snippet: Tagged protein was bound to 0.5 ml of glutathione beads (GST-tagged protein) or Ni-NTA (Qiagen) (his-tagged protein) in 50 ml tube with mixing at 4 °C for 2 h. The beads were packed onto a column and washed with wash buffer (1× protease inhibitor cocktail, 10 mM EDTA, 250 mMNaCl, and 0.5 mM DTT). .. Protein was concentrated by using Pierce™ Protein Concentrator PES, 3 K MWCO (ThermoFisher, Cat. No. 88514) and 30 K (ThermoFisher, Cat. No. 88529) for PAXX and PARP1 respectively in dialyzing buffer (50 mM Tris pH 7.9, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, and 10% glycerol) to lower the salt concentration.

    Protein Extraction:

    Article Title: Canavanine-Induced Decrease in Nitric Oxide Synthesis Alters Activity of Antioxidant System but Does Not Impact S-Nitrosoglutathione Catabolism in Tomato Roots
    Article Snippet: Paragraph title: Protein Extraction and Purification ... After centrifugation, supernatant was concentrated with Pierce™ Protein Concentrator PES, 3K MWCO (Thermo Scientific™ ) to obtain 2 ml of protein extract.

    Purification:

    Article Title: Canavanine-Induced Decrease in Nitric Oxide Synthesis Alters Activity of Antioxidant System but Does Not Impact S-Nitrosoglutathione Catabolism in Tomato Roots
    Article Snippet: Paragraph title: Protein Extraction and Purification ... After centrifugation, supernatant was concentrated with Pierce™ Protein Concentrator PES, 3K MWCO (Thermo Scientific™ ) to obtain 2 ml of protein extract.

    Article Title: PAXX Participates in Base Excision Repair via Interacting with Pol β and Contributes to TMZ Resistance in Glioma Cells
    Article Snippet: Paragraph title: Protein Expression and Purification ... Protein was concentrated by using Pierce™ Protein Concentrator PES, 3 K MWCO (ThermoFisher, Cat. No. 88514) and 30 K (ThermoFisher, Cat. No. 88529) for PAXX and PARP1 respectively in dialyzing buffer (50 mM Tris pH 7.9, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, and 10% glycerol) to lower the salt concentration.

    Concentration Assay:

    Article Title: PAXX Participates in Base Excision Repair via Interacting with Pol β and Contributes to TMZ Resistance in Glioma Cells
    Article Snippet: .. Protein was concentrated by using Pierce™ Protein Concentrator PES, 3 K MWCO (ThermoFisher, Cat. No. 88514) and 30 K (ThermoFisher, Cat. No. 88529) for PAXX and PARP1 respectively in dialyzing buffer (50 mM Tris pH 7.9, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, and 10% glycerol) to lower the salt concentration. ..

    Incubation:

    Article Title: Canavanine-Induced Decrease in Nitric Oxide Synthesis Alters Activity of Antioxidant System but Does Not Impact S-Nitrosoglutathione Catabolism in Tomato Roots
    Article Snippet: After centrifugation, supernatant was concentrated with Pierce™ Protein Concentrator PES, 3K MWCO (Thermo Scientific™ ) to obtain 2 ml of protein extract. .. Then, 5-µl protein G agarose (Thermo Scientific™ ) was added and incubated for 8 h at 4°C with gentle mixing.

    Expressing:

    Article Title: PAXX Participates in Base Excision Repair via Interacting with Pol β and Contributes to TMZ Resistance in Glioma Cells
    Article Snippet: Paragraph title: Protein Expression and Purification ... Protein was concentrated by using Pierce™ Protein Concentrator PES, 3 K MWCO (ThermoFisher, Cat. No. 88514) and 30 K (ThermoFisher, Cat. No. 88529) for PAXX and PARP1 respectively in dialyzing buffer (50 mM Tris pH 7.9, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, and 10% glycerol) to lower the salt concentration.

    Protein Concentration:

    Article Title: Functional Expression and One-Step Protein Purification of Manganese Peroxidase 1 (rMnP1) from Phanerochaetechrysosporium Using the E. coli-Expression System.
    Article Snippet: .. Quantification and Protein Sequencing of rMnP1 Prior to quantification, DTT was removed from the sample during protein concentration in a Pierce Protein Concentrator PES, 30K MWCO (Thermo Scientific). ..

    Sequencing:

    Article Title: Functional Expression and One-Step Protein Purification of Manganese Peroxidase 1 (rMnP1) from Phanerochaetechrysosporium Using the E. coli-Expression System.
    Article Snippet: .. Quantification and Protein Sequencing of rMnP1 Prior to quantification, DTT was removed from the sample during protein concentration in a Pierce Protein Concentrator PES, 30K MWCO (Thermo Scientific). ..

    Lysis:

    Article Title: PAXX Participates in Base Excision Repair via Interacting with Pol β and Contributes to TMZ Resistance in Glioma Cells
    Article Snippet: The medium was removed and the cell pellet was re-suspended in lysis buffer (1× protease inhibitor cocktail, 10 mM EDTA, 20 mM Tris pH 7.9, 600 mM NaCl, 2 mM DTT, 2 mg/ml lysozyme). .. Protein was concentrated by using Pierce™ Protein Concentrator PES, 3 K MWCO (ThermoFisher, Cat. No. 88514) and 30 K (ThermoFisher, Cat. No. 88529) for PAXX and PARP1 respectively in dialyzing buffer (50 mM Tris pH 7.9, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, and 10% glycerol) to lower the salt concentration.

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  • 99
    Thermo Fisher protein concentrator pes
    Protein Concentrator Pes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein concentrator pes/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    protein concentrator pes - by Bioz Stars, 2020-04
    99/100 stars
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