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Bio-Rad protein assay dye reagent concentrate
Protein Assay Dye Reagent Concentrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 4 article reviews
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protein assay dye reagent concentrate - by Bioz Stars, 2020-04
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Related Articles

Clone Assay:

Article Title: Two Novel α-l-Arabinofuranosidases from Bifidobacterium longum subsp. longum Belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan
Article Snippet: Paragraph title: Cloning, expression, and purification of enzymes. ... The protein concentration was measured using a Bio-Rad protein assay dye reagent concentrate (Bio-Rad).

Centrifugation:

Article Title: Tetracyclines Modify Translation By Targeting Key Human rRNA Substructures
Article Snippet: .. Lysates were vortexed intermittently while chilled on ice for 10 min and clarified by centrifugation at 14,000 x g. Protein concentrations of light and heavy lysates were estimated with the Protein Assay Dye Reagent Concentrate (Biorad) and equalized. ..

Article Title: Two Novel α-l-Arabinofuranosidases from Bifidobacterium longum subsp. longum Belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan
Article Snippet: Next, to induce expression, 0.1 mM isopropyl-β- d -thiogalactopyranoside (IPTG) was added to the culture, which was further incubated at 25°C for 15 h. The cells were harvested by centrifugation and lysed with the BugBuster protein extraction reagent (Novagen). .. The protein concentration was measured using a Bio-Rad protein assay dye reagent concentrate (Bio-Rad).

MTT Assay:

Article Title: Yes-associated protein 1 is required for proliferation and function of bovine granulosa cells in vitro †
Article Snippet: Mayer’s hematoxylin and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were from Sigma-Aldrich (St. Louis, MO, USA). .. Bio-Rad protein assay dye reagent concentrate is from Bio-Rad (Hercules, CA, USA).

Bradford Assay:

Article Title: RELATIONSHIP BETWEEN SELENOPROTEIN P AND SELENOCYSTEINE LYASE: INSIGHTS INTO SELENIUM METABOLISM
Article Snippet: .. Protein concentration was measured via Bradford assay using the Protein Assay Dye Reagent Concentrate (BioRad). .. 10–20 µg protein was loaded onto a 4–20% SDS-PAGE gel (BioRad), separated out by electrophoresis, and transferred to IPFL membranes (EMD Millipore).

Article Title: Insights into Innate Sensing of Prototype Foamy Viruses in Myeloid Cells
Article Snippet: .. Lysates were centrifuged (17,000× g for 15 min at 4 °C), and protein concentration was determined based on the Bradford assay using the Bio-Rad Protein Assay Dye Reagent Concentrate. .. Samples containing 20 µg protein were prepared with NuPAGE LDS sample buffer (4×) and NuPAGE Sample Reducing Agent (10×), to a final 1× concentration and denatured at 70 °C for 10 min. Proteins were separated on precasted NuPAGE™ 4–12% Bis-Tris gradient gels (Invitrogen).

Blocking Assay:

Article Title: A photo-cleavable surfactant for top-down proteomics
Article Snippet: Sodium orthovanadate, HPLC grade H2 O, acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), optima LC-MS grade formic acid, optima LC-MS grade isopropanol (IPA), Pierce protein-free tris-buffered saline (TBS) blocking buffer, tween 20, and molecular weight cutoff (10 and 30 kDa MWCO) (0.5 mL) centrifugal filters, Coomassie blue R-250, and Dulbecco’s modified eagle medium (DMEM) were purchased from Fisher Scientific (Waltham, MA). .. Protein Assay Dye Reagent Concentrate was purchased from BioRad (Hercules, CA).

Electrophoresis:

Article Title: RELATIONSHIP BETWEEN SELENOPROTEIN P AND SELENOCYSTEINE LYASE: INSIGHTS INTO SELENIUM METABOLISM
Article Snippet: Protein concentration was measured via Bradford assay using the Protein Assay Dye Reagent Concentrate (BioRad). .. 10–20 µg protein was loaded onto a 4–20% SDS-PAGE gel (BioRad), separated out by electrophoresis, and transferred to IPFL membranes (EMD Millipore).

Incubation:

Article Title: Adult-Onset Hepatocyte GH Resistance Promotes NASH in Male Mice, Without Severe Systemic Metabolic Dysfunction
Article Snippet: Adipose tissue was homogenized in RIPA buffer supplemented with phosphatase and protease inhibitor cocktails (Roche) and quantified using Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, Hercules, CA). .. Membranes were stained with Ponceau to ensure equal loading and incubated overnight at 4°C with nuclear factor κ B [NF- κ ( ); Abcam, Cambridge, UK], phosphorylated NF- κ ( ); Cell Signaling Technology].

Article Title: CX-5461 Inhibits Pancreatic Ductal Adenocarcinoma Cell Growth, Migration and Induces DNA Damage
Article Snippet: Western Blot Whole-cell lysates were prepared from cells treated with 1.5 μM CX-5461 for 24 h or medium as control, by addition of cell lysis buffer (#9803, Cell signaling, Leiden, The Netherlands) diluted in demineralized water and supplemented with sodium orthovanadate (S6508, Sigma-Aldrich, Zwijndrecht, The Netherlands) and protease inhibitor cocktail (11697498001, Sigma-Alrich, Zwijndrecht, The Netherlands), followed by incubation on ice for 30 min. .. The samples were spun down at 16,000× g for 10 min at 4 °C and the supernatant was used to perform the Bio-rad protein estimation using the protein assay dye reagent concentrate (#500-0006, Bio-Rad, Veenendaal, The Netherlands).

Article Title: Insights into Innate Sensing of Prototype Foamy Viruses in Myeloid Cells
Article Snippet: Lysates were centrifuged (17,000× g for 15 min at 4 °C), and protein concentration was determined based on the Bradford assay using the Bio-Rad Protein Assay Dye Reagent Concentrate. .. The gel was run in 1× MOPS buffer (1 M MOPS, 1 M Tris, 69.3 mM SDS, 20.5 mM EDTA Titriplex II) supplemented with 200 μL NuPage Antioxidant 10× (inner chamber) at 200 V for 1 h 10 min. Proteins were transferred to a Hybond P 0.45 PVDF membrane (GE Healthcare, Chicago, IL, USA) using the XCell IITM blotting system with 1× NuPAGE transfer buffer (Invitrogen) at 35 V for 1 h 40 min. Membranes were blocked in 5% (w /v ) BSA (Carl Roth) in 0.01% (v /v ) Tris-buffered saline with Tween 20 (TBST) for 2 h at 4 °C with subsequent incubation in primary antibody dilutions at 4 °C overnight.

Article Title: Two Novel α-l-Arabinofuranosidases from Bifidobacterium longum subsp. longum Belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan
Article Snippet: Next, to induce expression, 0.1 mM isopropyl-β- d -thiogalactopyranoside (IPTG) was added to the culture, which was further incubated at 25°C for 15 h. The cells were harvested by centrifugation and lysed with the BugBuster protein extraction reagent (Novagen). .. The protein concentration was measured using a Bio-Rad protein assay dye reagent concentrate (Bio-Rad).

Activity Assay:

Article Title: Hepatic HKDC1 Expression Contributes to Liver Metabolism
Article Snippet: HK activity was determined by the whole-cell lysate’s ability to phosphorylate glucose over 2 minutes in an assay mixture with final concentrations of 50 mM triethanolamine chloride, 7.5 mM MgCl2 , 0.5 mM EGTA, 11 mM monothioglycerol, 0.5 to 25 mM glucose, 6.6 mM ATP, 0.5 mg/mL NADP, and 0.5 U/mL G6PDH, pH 8.5. .. G6P formation was measured indirectly by NADPH production from G6PDH by measuring absorbance at 340 nm on a spectrophotometer and was normalized to protein concentration as determined using the Bio-Rad protein Assay Dye Reagent Concentrate.

Expressing:

Article Title: Insights into Innate Sensing of Prototype Foamy Viruses in Myeloid Cells
Article Snippet: Paragraph title: 2.8. Analysis of Cellular Protein Expression ... Lysates were centrifuged (17,000× g for 15 min at 4 °C), and protein concentration was determined based on the Bradford assay using the Bio-Rad Protein Assay Dye Reagent Concentrate.

Article Title: Two Novel α-l-Arabinofuranosidases from Bifidobacterium longum subsp. longum Belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan
Article Snippet: Paragraph title: Cloning, expression, and purification of enzymes. ... The protein concentration was measured using a Bio-Rad protein assay dye reagent concentrate (Bio-Rad).

BIA-KA:

Article Title: A photo-cleavable surfactant for top-down proteomics
Article Snippet: Goat Anti-Antigen: Rabbit IgG (H+L), Goat Anti-Antigen: mouse IgG (H+L), BCA protein assay, and Pierce 660 nm Protein Assay Reagent, Ionic Detergent Compatibility Reagent were purchased from Thermo Fisher (Waltham, MA). .. Protein Assay Dye Reagent Concentrate was purchased from BioRad (Hercules, CA).

Modification:

Article Title: A photo-cleavable surfactant for top-down proteomics
Article Snippet: Sodium orthovanadate, HPLC grade H2 O, acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), optima LC-MS grade formic acid, optima LC-MS grade isopropanol (IPA), Pierce protein-free tris-buffered saline (TBS) blocking buffer, tween 20, and molecular weight cutoff (10 and 30 kDa MWCO) (0.5 mL) centrifugal filters, Coomassie blue R-250, and Dulbecco’s modified eagle medium (DMEM) were purchased from Fisher Scientific (Waltham, MA). .. Protein Assay Dye Reagent Concentrate was purchased from BioRad (Hercules, CA).

Western Blot:

Article Title: Adult-Onset Hepatocyte GH Resistance Promotes NASH in Male Mice, Without Severe Systemic Metabolic Dysfunction
Article Snippet: Adipose tissue was homogenized in RIPA buffer supplemented with phosphatase and protease inhibitor cocktails (Roche) and quantified using Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, Hercules, CA). .. Immunoreactive bands were visualized using the chemiluminescence horseradish peroxidase substrate (EMD Millipore, Billerica, MA) or Clarity Western ECL Substrate (Bio-Rad Laboratories), and densitometry analyses were performed with ImageJ software (National Institutes of Health, Bethesda, MD) or Image Laboratory (Bio-Rad Laboratories).

Article Title: CX-5461 Inhibits Pancreatic Ductal Adenocarcinoma Cell Growth, Migration and Induces DNA Damage
Article Snippet: Paragraph title: 4.7. Western Blot ... The samples were spun down at 16,000× g for 10 min at 4 °C and the supernatant was used to perform the Bio-rad protein estimation using the protein assay dye reagent concentrate (#500-0006, Bio-Rad, Veenendaal, The Netherlands).

Article Title: RELATIONSHIP BETWEEN SELENOPROTEIN P AND SELENOCYSTEINE LYASE: INSIGHTS INTO SELENIUM METABOLISM
Article Snippet: Paragraph title: Western Blot and antibodies ... Protein concentration was measured via Bradford assay using the Protein Assay Dye Reagent Concentrate (BioRad).

Transformation Assay:

Article Title: Two Novel α-l-Arabinofuranosidases from Bifidobacterium longum subsp. longum Belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan
Article Snippet: The E. coli BL21(λDE3) Δ lacZ strain was transformed with these plasmids and cultured in LB liquid medium containing 100 μg/ml ampicillin at 37°C under aerobic conditions until the OD600 reached 0.35. .. The protein concentration was measured using a Bio-Rad protein assay dye reagent concentrate (Bio-Rad).

High Performance Liquid Chromatography:

Article Title: A photo-cleavable surfactant for top-down proteomics
Article Snippet: Sodium orthovanadate, HPLC grade H2 O, acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), optima LC-MS grade formic acid, optima LC-MS grade isopropanol (IPA), Pierce protein-free tris-buffered saline (TBS) blocking buffer, tween 20, and molecular weight cutoff (10 and 30 kDa MWCO) (0.5 mL) centrifugal filters, Coomassie blue R-250, and Dulbecco’s modified eagle medium (DMEM) were purchased from Fisher Scientific (Waltham, MA). .. Protein Assay Dye Reagent Concentrate was purchased from BioRad (Hercules, CA).

Electron Microscopy:

Article Title: Yes-associated protein 1 is required for proliferation and function of bovine granulosa cells in vitro †
Article Snippet: Fluoromount-G and clear nail polish were purchased from Electron Microscopy Sciences (Hatfield, PA, USA). .. Bio-Rad protein assay dye reagent concentrate is from Bio-Rad (Hercules, CA, USA).

Protease Inhibitor:

Article Title: Adult-Onset Hepatocyte GH Resistance Promotes NASH in Male Mice, Without Severe Systemic Metabolic Dysfunction
Article Snippet: .. Adipose tissue was homogenized in RIPA buffer supplemented with phosphatase and protease inhibitor cocktails (Roche) and quantified using Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, Hercules, CA). .. Equal amounts of isolated proteins were separated into precast SDS-PAGE gels in reducing conditions and transferred to nitrocellulose membranes.

Article Title: CX-5461 Inhibits Pancreatic Ductal Adenocarcinoma Cell Growth, Migration and Induces DNA Damage
Article Snippet: Western Blot Whole-cell lysates were prepared from cells treated with 1.5 μM CX-5461 for 24 h or medium as control, by addition of cell lysis buffer (#9803, Cell signaling, Leiden, The Netherlands) diluted in demineralized water and supplemented with sodium orthovanadate (S6508, Sigma-Aldrich, Zwijndrecht, The Netherlands) and protease inhibitor cocktail (11697498001, Sigma-Alrich, Zwijndrecht, The Netherlands), followed by incubation on ice for 30 min. .. The samples were spun down at 16,000× g for 10 min at 4 °C and the supernatant was used to perform the Bio-rad protein estimation using the protein assay dye reagent concentrate (#500-0006, Bio-Rad, Veenendaal, The Netherlands).

Article Title: A photo-cleavable surfactant for top-down proteomics
Article Snippet: HEPES, ammonium bicarbonate (NH4 HCO3 ), sucrose, sodium fluoride (NaF), phenylmethanesulfonyl fluoride (PMSF), ethylenediaminetetraacetic acid (EDTA), n-dodecyl β-D-maltoside (DDM), octyl β-D-glucopyranoside (OG), sodium dodecyl sulfate (SDS), digitonin (DGT), protease inhibitor cocktail, tri(2-carboxyethyl) phosphine hydrochloride (TCEP), dithiothreitol (DTT), 2-mercaptoethanol (2-ME), β-casein from bovine milk, ubiquitin from bovine erythrocytes (Ubi), bovine serum albumin (BSA), myoglobin from equine heart (Myo) and cytochrome c (Cytc ) from equine heart, ribonuclease A (RNase A) and ribonuclease B (RNaseB) from bovine pancreas were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). .. Protein Assay Dye Reagent Concentrate was purchased from BioRad (Hercules, CA).

Article Title: RELATIONSHIP BETWEEN SELENOPROTEIN P AND SELENOCYSTEINE LYASE: INSIGHTS INTO SELENIUM METABOLISM
Article Snippet: Protein was collected in CelLytic (Sigma) with a 1:100 ratio of Protease Inhibitor Cocktail Set III, EDTA-Free (EMD Millipore) for both tissue and cell lines. .. Protein concentration was measured via Bradford assay using the Protein Assay Dye Reagent Concentrate (BioRad).

Article Title: Insights into Innate Sensing of Prototype Foamy Viruses in Myeloid Cells
Article Snippet: Six or twenty-four hours post-exposure, cells were harvested by resuspension in ice cold PBS, centrifuged (300× g , 6 min, 4 °C), and lysed in 25 µL RIPA-lysis buffer (100 mM NaCl; 10 mM EDTA (pH 7.5), 20 mM Tris (pH 7.5); 1% (v /v ) Triton X-100; 1% (w /v ) sodium deoxycholate) containing protease and phosphatase inhibitor cocktails (Complete Protease Inhibitor Cocktail; PhosSTOP Phosphatase Inhibitor Cocktail, Roche) for 45 min on ice. .. Lysates were centrifuged (17,000× g for 15 min at 4 °C), and protein concentration was determined based on the Bradford assay using the Bio-Rad Protein Assay Dye Reagent Concentrate.

Cell Culture:

Article Title: Two Novel α-l-Arabinofuranosidases from Bifidobacterium longum subsp. longum Belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan
Article Snippet: The E. coli BL21(λDE3) Δ lacZ strain was transformed with these plasmids and cultured in LB liquid medium containing 100 μg/ml ampicillin at 37°C under aerobic conditions until the OD600 reached 0.35. .. The protein concentration was measured using a Bio-Rad protein assay dye reagent concentrate (Bio-Rad).

Indirect Immunoperoxidase Assay:

Article Title: A photo-cleavable surfactant for top-down proteomics
Article Snippet: Sodium orthovanadate, HPLC grade H2 O, acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), optima LC-MS grade formic acid, optima LC-MS grade isopropanol (IPA), Pierce protein-free tris-buffered saline (TBS) blocking buffer, tween 20, and molecular weight cutoff (10 and 30 kDa MWCO) (0.5 mL) centrifugal filters, Coomassie blue R-250, and Dulbecco’s modified eagle medium (DMEM) were purchased from Fisher Scientific (Waltham, MA). .. Protein Assay Dye Reagent Concentrate was purchased from BioRad (Hercules, CA).

Protein Concentration:

Article Title: Hepatic HKDC1 Expression Contributes to Liver Metabolism
Article Snippet: .. G6P formation was measured indirectly by NADPH production from G6PDH by measuring absorbance at 340 nm on a spectrophotometer and was normalized to protein concentration as determined using the Bio-Rad protein Assay Dye Reagent Concentrate. .. In brief, Rosetta 2 (DE3; EMD Millipore) cells were transformed with pReceiver-B01 plasmid containing HKDC1 , HK1 , or GCK .

Article Title: RELATIONSHIP BETWEEN SELENOPROTEIN P AND SELENOCYSTEINE LYASE: INSIGHTS INTO SELENIUM METABOLISM
Article Snippet: .. Protein concentration was measured via Bradford assay using the Protein Assay Dye Reagent Concentrate (BioRad). .. 10–20 µg protein was loaded onto a 4–20% SDS-PAGE gel (BioRad), separated out by electrophoresis, and transferred to IPFL membranes (EMD Millipore).

Article Title: Insights into Innate Sensing of Prototype Foamy Viruses in Myeloid Cells
Article Snippet: .. Lysates were centrifuged (17,000× g for 15 min at 4 °C), and protein concentration was determined based on the Bradford assay using the Bio-Rad Protein Assay Dye Reagent Concentrate. .. Samples containing 20 µg protein were prepared with NuPAGE LDS sample buffer (4×) and NuPAGE Sample Reducing Agent (10×), to a final 1× concentration and denatured at 70 °C for 10 min. Proteins were separated on precasted NuPAGE™ 4–12% Bis-Tris gradient gels (Invitrogen).

Article Title: Abrogating mitochondrial dynamics in mouse hearts accelerates mitochondrial senescence
Article Snippet: .. Protein concentration of the isolated cardiac mitochondria was measured with protein assay dye reagent concentrate (Bio-Rad, 500-0006) using a spectrophotometer. .. Total DNAs were extracted from cardiac tissues with DNeasy blood & tissue kit (Qiagen, 69506).

Article Title: Global Secretome Characterization of the Pathogenic Yeast Candida glabrata
Article Snippet: .. The protein concentration in collected secretome samples was measured using the Bio-Rad Protein Assay Dye Reagent Concentrate. .. Global Secretome Analysis The global secretome analysis involved protein identification via the microcapillary LC-MS/MS (liquid chromatography-tandem mass spectrometry) method.

Article Title: Two Novel α-l-Arabinofuranosidases from Bifidobacterium longum subsp. longum Belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan
Article Snippet: .. The protein concentration was measured using a Bio-Rad protein assay dye reagent concentrate (Bio-Rad). .. α- l -Arabinofuranosidase activity was measured based on the release of p -nitrophenyl (pNP) from pNP-α-Ara f .

Sequencing:

Article Title: Two Novel α-l-Arabinofuranosidases from Bifidobacterium longum subsp. longum Belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan
Article Snippet: The nucleotide sequences were confirmed by sequencing. .. The protein concentration was measured using a Bio-Rad protein assay dye reagent concentrate (Bio-Rad).

Sonication:

Article Title: Hepatic HKDC1 Expression Contributes to Liver Metabolism
Article Snippet: Cells were lysed by sonication, five pulses of 1 second, in 100 µl homogenization buffer: 0.2% Triton X-100, 0.5 mM EGTA, 10 mM D-(+)-glucose, 11.1 mM monothioglycerol, 45 mM Tris-HCl (pH 8.2), and 50 mM KH2 PO4 . .. G6P formation was measured indirectly by NADPH production from G6PDH by measuring absorbance at 340 nm on a spectrophotometer and was normalized to protein concentration as determined using the Bio-Rad protein Assay Dye Reagent Concentrate.

Article Title: RELATIONSHIP BETWEEN SELENOPROTEIN P AND SELENOCYSTEINE LYASE: INSIGHTS INTO SELENIUM METABOLISM
Article Snippet: Tissues were sonicated 30–90 pulses, depending on the tissue, using a Sonic Dismembrator Model 100 (Fisher Scientific). .. Protein concentration was measured via Bradford assay using the Protein Assay Dye Reagent Concentrate (BioRad).

Molecular Weight:

Article Title: A photo-cleavable surfactant for top-down proteomics
Article Snippet: Sodium orthovanadate, HPLC grade H2 O, acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), optima LC-MS grade formic acid, optima LC-MS grade isopropanol (IPA), Pierce protein-free tris-buffered saline (TBS) blocking buffer, tween 20, and molecular weight cutoff (10 and 30 kDa MWCO) (0.5 mL) centrifugal filters, Coomassie blue R-250, and Dulbecco’s modified eagle medium (DMEM) were purchased from Fisher Scientific (Waltham, MA). .. Protein Assay Dye Reagent Concentrate was purchased from BioRad (Hercules, CA).

Nucleic Acid Electrophoresis:

Article Title: Global Secretome Characterization of the Pathogenic Yeast Candida glabrata
Article Snippet: The concentrated secretome fractions (200 μg) were resolved on a 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and visualized with the Coomassie Brilliant Blue stain. .. The protein concentration in collected secretome samples was measured using the Bio-Rad Protein Assay Dye Reagent Concentrate.

Isolation:

Article Title: Adult-Onset Hepatocyte GH Resistance Promotes NASH in Male Mice, Without Severe Systemic Metabolic Dysfunction
Article Snippet: Adipose tissue was homogenized in RIPA buffer supplemented with phosphatase and protease inhibitor cocktails (Roche) and quantified using Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, Hercules, CA). .. Equal amounts of isolated proteins were separated into precast SDS-PAGE gels in reducing conditions and transferred to nitrocellulose membranes.

Article Title: Tetracyclines Modify Translation By Targeting Key Human rRNA Substructures
Article Snippet: Paragraph title: SILAC Affinity Isolation and LC-MS/MS Analysis ... Lysates were vortexed intermittently while chilled on ice for 10 min and clarified by centrifugation at 14,000 x g. Protein concentrations of light and heavy lysates were estimated with the Protein Assay Dye Reagent Concentrate (Biorad) and equalized.

Article Title: Abrogating mitochondrial dynamics in mouse hearts accelerates mitochondrial senescence
Article Snippet: .. Protein concentration of the isolated cardiac mitochondria was measured with protein assay dye reagent concentrate (Bio-Rad, 500-0006) using a spectrophotometer. .. Total DNAs were extracted from cardiac tissues with DNeasy blood & tissue kit (Qiagen, 69506).

Labeling:

Article Title: Yes-associated protein 1 is required for proliferation and function of bovine granulosa cells in vitro †
Article Snippet: Bio-Rad protein assay dye reagent concentrate is from Bio-Rad (Hercules, CA, USA). .. Biotin was added to phosphorylated YAP1 (Ser127) polyclonal antibody ( ) using a commercially available kit per manufacture’s protocol (DSB-X Biotin Protein Labeling Kit; Cayman Chemical Company).

Purification:

Article Title: Two Novel α-l-Arabinofuranosidases from Bifidobacterium longum subsp. longum Belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan
Article Snippet: Paragraph title: Cloning, expression, and purification of enzymes. ... The protein concentration was measured using a Bio-Rad protein assay dye reagent concentrate (Bio-Rad).

Small Interfering RNA:

Article Title: Yes-associated protein 1 is required for proliferation and function of bovine granulosa cells in vitro †
Article Snippet: Yes-associated protein 1 small interfering RNA (siRNA) was from Dhamarcon/Thermo Scientific (Pittsburgh, PA, USA). .. Bio-Rad protein assay dye reagent concentrate is from Bio-Rad (Hercules, CA, USA).

Staining:

Article Title: Adult-Onset Hepatocyte GH Resistance Promotes NASH in Male Mice, Without Severe Systemic Metabolic Dysfunction
Article Snippet: Adipose tissue was homogenized in RIPA buffer supplemented with phosphatase and protease inhibitor cocktails (Roche) and quantified using Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, Hercules, CA). .. Membranes were stained with Ponceau to ensure equal loading and incubated overnight at 4°C with nuclear factor κ B [NF- κ ( ); Abcam, Cambridge, UK], phosphorylated NF- κ ( ); Cell Signaling Technology].

Article Title: Global Secretome Characterization of the Pathogenic Yeast Candida glabrata
Article Snippet: The concentrated secretome fractions (200 μg) were resolved on a 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and visualized with the Coomassie Brilliant Blue stain. .. The protein concentration in collected secretome samples was measured using the Bio-Rad Protein Assay Dye Reagent Concentrate.

SDS Page:

Article Title: Adult-Onset Hepatocyte GH Resistance Promotes NASH in Male Mice, Without Severe Systemic Metabolic Dysfunction
Article Snippet: Adipose tissue was homogenized in RIPA buffer supplemented with phosphatase and protease inhibitor cocktails (Roche) and quantified using Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, Hercules, CA). .. Equal amounts of isolated proteins were separated into precast SDS-PAGE gels in reducing conditions and transferred to nitrocellulose membranes.

Article Title: RELATIONSHIP BETWEEN SELENOPROTEIN P AND SELENOCYSTEINE LYASE: INSIGHTS INTO SELENIUM METABOLISM
Article Snippet: Protein concentration was measured via Bradford assay using the Protein Assay Dye Reagent Concentrate (BioRad). .. 10–20 µg protein was loaded onto a 4–20% SDS-PAGE gel (BioRad), separated out by electrophoresis, and transferred to IPFL membranes (EMD Millipore).

Article Title: Global Secretome Characterization of the Pathogenic Yeast Candida glabrata
Article Snippet: The concentrated secretome fractions (200 μg) were resolved on a 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and visualized with the Coomassie Brilliant Blue stain. .. The protein concentration in collected secretome samples was measured using the Bio-Rad Protein Assay Dye Reagent Concentrate.

Software:

Article Title: Adult-Onset Hepatocyte GH Resistance Promotes NASH in Male Mice, Without Severe Systemic Metabolic Dysfunction
Article Snippet: Adipose tissue was homogenized in RIPA buffer supplemented with phosphatase and protease inhibitor cocktails (Roche) and quantified using Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, Hercules, CA). .. Immunoreactive bands were visualized using the chemiluminescence horseradish peroxidase substrate (EMD Millipore, Billerica, MA) or Clarity Western ECL Substrate (Bio-Rad Laboratories), and densitometry analyses were performed with ImageJ software (National Institutes of Health, Bethesda, MD) or Image Laboratory (Bio-Rad Laboratories).

Homogenization:

Article Title: Hepatic HKDC1 Expression Contributes to Liver Metabolism
Article Snippet: Cells were lysed by sonication, five pulses of 1 second, in 100 µl homogenization buffer: 0.2% Triton X-100, 0.5 mM EGTA, 10 mM D-(+)-glucose, 11.1 mM monothioglycerol, 45 mM Tris-HCl (pH 8.2), and 50 mM KH2 PO4 . .. G6P formation was measured indirectly by NADPH production from G6PDH by measuring absorbance at 340 nm on a spectrophotometer and was normalized to protein concentration as determined using the Bio-Rad protein Assay Dye Reagent Concentrate.

Histone Deacetylase Assay:

Article Title: Adult-Onset Hepatocyte GH Resistance Promotes NASH in Male Mice, Without Severe Systemic Metabolic Dysfunction
Article Snippet: Liver or adipose tissue was homogenized in RIPA buffer supplemented with EDTA, protease and phosphatase inhibitor cocktails (Roche), and a lysine deacetylase inhibitor cocktail (10 mM trichostatin A, 10 mM nicotinamide, and 50 mM butyric acid, all from Sigma-Aldrich, Madison, WI). .. Adipose tissue was homogenized in RIPA buffer supplemented with phosphatase and protease inhibitor cocktails (Roche) and quantified using Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, Hercules, CA).

Protein Extraction:

Article Title: Two Novel α-l-Arabinofuranosidases from Bifidobacterium longum subsp. longum Belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan
Article Snippet: Next, to induce expression, 0.1 mM isopropyl-β- d -thiogalactopyranoside (IPTG) was added to the culture, which was further incubated at 25°C for 15 h. The cells were harvested by centrifugation and lysed with the BugBuster protein extraction reagent (Novagen). .. The protein concentration was measured using a Bio-Rad protein assay dye reagent concentrate (Bio-Rad).

Spectrophotometry:

Article Title: Hepatic HKDC1 Expression Contributes to Liver Metabolism
Article Snippet: .. G6P formation was measured indirectly by NADPH production from G6PDH by measuring absorbance at 340 nm on a spectrophotometer and was normalized to protein concentration as determined using the Bio-Rad protein Assay Dye Reagent Concentrate. .. In brief, Rosetta 2 (DE3; EMD Millipore) cells were transformed with pReceiver-B01 plasmid containing HKDC1 , HK1 , or GCK .

Article Title: Abrogating mitochondrial dynamics in mouse hearts accelerates mitochondrial senescence
Article Snippet: .. Protein concentration of the isolated cardiac mitochondria was measured with protein assay dye reagent concentrate (Bio-Rad, 500-0006) using a spectrophotometer. .. Total DNAs were extracted from cardiac tissues with DNeasy blood & tissue kit (Qiagen, 69506).

Acid Assay:

Article Title: Adult-Onset Hepatocyte GH Resistance Promotes NASH in Male Mice, Without Severe Systemic Metabolic Dysfunction
Article Snippet: Protein determination was performed using the bicinchoninic acid assay (Thermo Scientific, Waltham, MA). .. Adipose tissue was homogenized in RIPA buffer supplemented with phosphatase and protease inhibitor cocktails (Roche) and quantified using Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, Hercules, CA).

Concentration Assay:

Article Title: Insights into Innate Sensing of Prototype Foamy Viruses in Myeloid Cells
Article Snippet: Lysates were centrifuged (17,000× g for 15 min at 4 °C), and protein concentration was determined based on the Bradford assay using the Bio-Rad Protein Assay Dye Reagent Concentrate. .. Samples containing 20 µg protein were prepared with NuPAGE LDS sample buffer (4×) and NuPAGE Sample Reducing Agent (10×), to a final 1× concentration and denatured at 70 °C for 10 min. Proteins were separated on precasted NuPAGE™ 4–12% Bis-Tris gradient gels (Invitrogen).

Article Title: Two Novel α-l-Arabinofuranosidases from Bifidobacterium longum subsp. longum Belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan
Article Snippet: The cell-free protein extract was applied to a His GraviTrap column (GE Healthcare, USA), and the adsorbed proteins were eluted by a stepwise imidazole concentration gradient in 20 mM sodium acetate buffer at pH 6.5 containing 500 mM NaCl. .. The protein concentration was measured using a Bio-Rad protein assay dye reagent concentrate (Bio-Rad).

Thin Layer Chromatography:

Article Title: Treatment of human skeletal muscle cells with inhibitors of diacylglycerol acyltransferases 1 and 2 to explore isozyme-specific roles on lipid metabolism
Article Snippet: Thin layer chromatography plates were purchased from Merck (Darmstadt, Germany). .. Bio-Rad Protein Assay Dye Reagent Concentrate was from Bio-Rad (Copenhagen, Denmark).

Liquid Chromatography with Mass Spectroscopy:

Article Title: A photo-cleavable surfactant for top-down proteomics
Article Snippet: Sodium orthovanadate, HPLC grade H2 O, acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), optima LC-MS grade formic acid, optima LC-MS grade isopropanol (IPA), Pierce protein-free tris-buffered saline (TBS) blocking buffer, tween 20, and molecular weight cutoff (10 and 30 kDa MWCO) (0.5 mL) centrifugal filters, Coomassie blue R-250, and Dulbecco’s modified eagle medium (DMEM) were purchased from Fisher Scientific (Waltham, MA). .. Protein Assay Dye Reagent Concentrate was purchased from BioRad (Hercules, CA).

Lysis:

Article Title: CX-5461 Inhibits Pancreatic Ductal Adenocarcinoma Cell Growth, Migration and Induces DNA Damage
Article Snippet: Western Blot Whole-cell lysates were prepared from cells treated with 1.5 μM CX-5461 for 24 h or medium as control, by addition of cell lysis buffer (#9803, Cell signaling, Leiden, The Netherlands) diluted in demineralized water and supplemented with sodium orthovanadate (S6508, Sigma-Aldrich, Zwijndrecht, The Netherlands) and protease inhibitor cocktail (11697498001, Sigma-Alrich, Zwijndrecht, The Netherlands), followed by incubation on ice for 30 min. .. The samples were spun down at 16,000× g for 10 min at 4 °C and the supernatant was used to perform the Bio-rad protein estimation using the protein assay dye reagent concentrate (#500-0006, Bio-Rad, Veenendaal, The Netherlands).

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    Bio-Rad microassay procedure
    Microassay Procedure, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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