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Millipore protein a sepharose 4b
Protein A Sepharose 4b, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein a sepharose 4b/product/Millipore
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
protein a sepharose 4b - by Bioz Stars, 2022-11
93/100 stars

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  • 90
    Millipore protein a g agarose beads
    Replication of rMDVs in tissue culture cells and expression of pCHPK and pUS10. (A) Plaque areas were measured for rMDVs reconstituted from each parental virus (vCHPKwt, vCHPKmut, vΔCHPK) and HA-tagged US10 virus (vCHPKwt/10HA, vCHPKmut/10HA, vΔCHPK/10HA). There were no significant differences between all groups using One-way ANOVA ( P = 0.71, n = 300) and Levene’s Test ( P = 0.84, n = 300). (B) Representative plaques induced by each virus stained with anti-MDV, -Flag, and -HA antibodies. Included in each image is Hoechst 33342 staining of nuclei for reference. (C) Confocal microscopy of vCHPKwt/10HA-, vCHPKmut/10HA-, or vΔCHPK/10HA-infected CECS. Cells were stained for Flag (CHPK) and HA (US10). (D) Immunoblotting of total protein collected from mock-infected CECs or CECs infected with vCHPKwt/10HA, vCHPKmut/10HA, or vΔCHPK/10HA. To examine CHPK and US10 expression, proteins were electrophoresed under non-reducing conditions and blotted with anti-Flag (CHPK) and -HA (US10) antibodies. Protein was also run under reducing conditions (*) and blotted with anti-β-actin and -MDV pp38 as protein loading and infection level controls, respectively. (E) Confocal images of CECs infected with vCHPKwt/10HA were used to quantify the colocalization of pCHPK and pUS10. A representative image of a plaque stained with anti-Flag (pCHPKwt) and -HA (pUS10) and merged. Hoechst was used to identify cell nuclei. A single z-section with linear ROIs labeled and the profile plot for ROI-1, and its Pearson Correlation Coefficient R is shown. The average Pearson Correlation Coefficient R between pCHPKwt and pUS10 was 0.8859 ± 0.0638. (F) Uninfected CECs (Mock) or CECs infected with vCHPKwt/10HA were used for protein extraction. Total cell lysate was immunoblotted for pCHPKwt (Flag), pUS10 (HA), VP5 (non-specific control), and β-actin (protein loading control). Total lysate was immunoprecipitated with anti-Flag Fab or anti-HA combined with protein <t>A/G-beads,</t> electrophoresed on an SDS-PAGE gel, and then immunoblotted using anti-Flag, -HA, or VP5 antibodies.
    Protein A G Agarose Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein a g agarose beads/product/Millipore
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    protein a g agarose beads - by Bioz Stars, 2022-11
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    88
    Millipore protein a sepharose beads
    Replication of rMDVs in tissue culture cells and expression of pCHPK and pUS10. (A) Plaque areas were measured for rMDVs reconstituted from each parental virus (vCHPKwt, vCHPKmut, vΔCHPK) and HA-tagged US10 virus (vCHPKwt/10HA, vCHPKmut/10HA, vΔCHPK/10HA). There were no significant differences between all groups using One-way ANOVA ( P = 0.71, n = 300) and Levene’s Test ( P = 0.84, n = 300). (B) Representative plaques induced by each virus stained with anti-MDV, -Flag, and -HA antibodies. Included in each image is Hoechst 33342 staining of nuclei for reference. (C) Confocal microscopy of vCHPKwt/10HA-, vCHPKmut/10HA-, or vΔCHPK/10HA-infected CECS. Cells were stained for Flag (CHPK) and HA (US10). (D) Immunoblotting of total protein collected from mock-infected CECs or CECs infected with vCHPKwt/10HA, vCHPKmut/10HA, or vΔCHPK/10HA. To examine CHPK and US10 expression, proteins were electrophoresed under non-reducing conditions and blotted with anti-Flag (CHPK) and -HA (US10) antibodies. Protein was also run under reducing conditions (*) and blotted with anti-β-actin and -MDV pp38 as protein loading and infection level controls, respectively. (E) Confocal images of CECs infected with vCHPKwt/10HA were used to quantify the colocalization of pCHPK and pUS10. A representative image of a plaque stained with anti-Flag (pCHPKwt) and -HA (pUS10) and merged. Hoechst was used to identify cell nuclei. A single z-section with linear ROIs labeled and the profile plot for ROI-1, and its Pearson Correlation Coefficient R is shown. The average Pearson Correlation Coefficient R between pCHPKwt and pUS10 was 0.8859 ± 0.0638. (F) Uninfected CECs (Mock) or CECs infected with vCHPKwt/10HA were used for protein extraction. Total cell lysate was immunoblotted for pCHPKwt (Flag), pUS10 (HA), VP5 (non-specific control), and β-actin (protein loading control). Total lysate was immunoprecipitated with anti-Flag Fab or anti-HA combined with protein <t>A/G-beads,</t> electrophoresed on an SDS-PAGE gel, and then immunoblotted using anti-Flag, -HA, or VP5 antibodies.
    Protein A Sepharose Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein a sepharose beads/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protein a sepharose beads - by Bioz Stars, 2022-11
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    92
    Millipore gammabind plus sepharose
    Replication of rMDVs in tissue culture cells and expression of pCHPK and pUS10. (A) Plaque areas were measured for rMDVs reconstituted from each parental virus (vCHPKwt, vCHPKmut, vΔCHPK) and HA-tagged US10 virus (vCHPKwt/10HA, vCHPKmut/10HA, vΔCHPK/10HA). There were no significant differences between all groups using One-way ANOVA ( P = 0.71, n = 300) and Levene’s Test ( P = 0.84, n = 300). (B) Representative plaques induced by each virus stained with anti-MDV, -Flag, and -HA antibodies. Included in each image is Hoechst 33342 staining of nuclei for reference. (C) Confocal microscopy of vCHPKwt/10HA-, vCHPKmut/10HA-, or vΔCHPK/10HA-infected CECS. Cells were stained for Flag (CHPK) and HA (US10). (D) Immunoblotting of total protein collected from mock-infected CECs or CECs infected with vCHPKwt/10HA, vCHPKmut/10HA, or vΔCHPK/10HA. To examine CHPK and US10 expression, proteins were electrophoresed under non-reducing conditions and blotted with anti-Flag (CHPK) and -HA (US10) antibodies. Protein was also run under reducing conditions (*) and blotted with anti-β-actin and -MDV pp38 as protein loading and infection level controls, respectively. (E) Confocal images of CECs infected with vCHPKwt/10HA were used to quantify the colocalization of pCHPK and pUS10. A representative image of a plaque stained with anti-Flag (pCHPKwt) and -HA (pUS10) and merged. Hoechst was used to identify cell nuclei. A single z-section with linear ROIs labeled and the profile plot for ROI-1, and its Pearson Correlation Coefficient R is shown. The average Pearson Correlation Coefficient R between pCHPKwt and pUS10 was 0.8859 ± 0.0638. (F) Uninfected CECs (Mock) or CECs infected with vCHPKwt/10HA were used for protein extraction. Total cell lysate was immunoblotted for pCHPKwt (Flag), pUS10 (HA), VP5 (non-specific control), and β-actin (protein loading control). Total lysate was immunoprecipitated with anti-Flag Fab or anti-HA combined with protein <t>A/G-beads,</t> electrophoresed on an SDS-PAGE gel, and then immunoblotted using anti-Flag, -HA, or VP5 antibodies.
    Gammabind Plus Sepharose, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gammabind plus sepharose/product/Millipore
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    gammabind plus sepharose - by Bioz Stars, 2022-11
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    80
    Millipore slurry protein a g plus agarose beads
    Replication of rMDVs in tissue culture cells and expression of pCHPK and pUS10. (A) Plaque areas were measured for rMDVs reconstituted from each parental virus (vCHPKwt, vCHPKmut, vΔCHPK) and HA-tagged US10 virus (vCHPKwt/10HA, vCHPKmut/10HA, vΔCHPK/10HA). There were no significant differences between all groups using One-way ANOVA ( P = 0.71, n = 300) and Levene’s Test ( P = 0.84, n = 300). (B) Representative plaques induced by each virus stained with anti-MDV, -Flag, and -HA antibodies. Included in each image is Hoechst 33342 staining of nuclei for reference. (C) Confocal microscopy of vCHPKwt/10HA-, vCHPKmut/10HA-, or vΔCHPK/10HA-infected CECS. Cells were stained for Flag (CHPK) and HA (US10). (D) Immunoblotting of total protein collected from mock-infected CECs or CECs infected with vCHPKwt/10HA, vCHPKmut/10HA, or vΔCHPK/10HA. To examine CHPK and US10 expression, proteins were electrophoresed under non-reducing conditions and blotted with anti-Flag (CHPK) and -HA (US10) antibodies. Protein was also run under reducing conditions (*) and blotted with anti-β-actin and -MDV pp38 as protein loading and infection level controls, respectively. (E) Confocal images of CECs infected with vCHPKwt/10HA were used to quantify the colocalization of pCHPK and pUS10. A representative image of a plaque stained with anti-Flag (pCHPKwt) and -HA (pUS10) and merged. Hoechst was used to identify cell nuclei. A single z-section with linear ROIs labeled and the profile plot for ROI-1, and its Pearson Correlation Coefficient R is shown. The average Pearson Correlation Coefficient R between pCHPKwt and pUS10 was 0.8859 ± 0.0638. (F) Uninfected CECs (Mock) or CECs infected with vCHPKwt/10HA were used for protein extraction. Total cell lysate was immunoblotted for pCHPKwt (Flag), pUS10 (HA), VP5 (non-specific control), and β-actin (protein loading control). Total lysate was immunoprecipitated with anti-Flag Fab or anti-HA combined with protein <t>A/G-beads,</t> electrophoresed on an SDS-PAGE gel, and then immunoblotted using anti-Flag, -HA, or VP5 antibodies.
    Slurry Protein A G Plus Agarose Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/slurry protein a g plus agarose beads/product/Millipore
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    slurry protein a g plus agarose beads - by Bioz Stars, 2022-11
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    Replication of rMDVs in tissue culture cells and expression of pCHPK and pUS10. (A) Plaque areas were measured for rMDVs reconstituted from each parental virus (vCHPKwt, vCHPKmut, vΔCHPK) and HA-tagged US10 virus (vCHPKwt/10HA, vCHPKmut/10HA, vΔCHPK/10HA). There were no significant differences between all groups using One-way ANOVA ( P = 0.71, n = 300) and Levene’s Test ( P = 0.84, n = 300). (B) Representative plaques induced by each virus stained with anti-MDV, -Flag, and -HA antibodies. Included in each image is Hoechst 33342 staining of nuclei for reference. (C) Confocal microscopy of vCHPKwt/10HA-, vCHPKmut/10HA-, or vΔCHPK/10HA-infected CECS. Cells were stained for Flag (CHPK) and HA (US10). (D) Immunoblotting of total protein collected from mock-infected CECs or CECs infected with vCHPKwt/10HA, vCHPKmut/10HA, or vΔCHPK/10HA. To examine CHPK and US10 expression, proteins were electrophoresed under non-reducing conditions and blotted with anti-Flag (CHPK) and -HA (US10) antibodies. Protein was also run under reducing conditions (*) and blotted with anti-β-actin and -MDV pp38 as protein loading and infection level controls, respectively. (E) Confocal images of CECs infected with vCHPKwt/10HA were used to quantify the colocalization of pCHPK and pUS10. A representative image of a plaque stained with anti-Flag (pCHPKwt) and -HA (pUS10) and merged. Hoechst was used to identify cell nuclei. A single z-section with linear ROIs labeled and the profile plot for ROI-1, and its Pearson Correlation Coefficient R is shown. The average Pearson Correlation Coefficient R between pCHPKwt and pUS10 was 0.8859 ± 0.0638. (F) Uninfected CECs (Mock) or CECs infected with vCHPKwt/10HA were used for protein extraction. Total cell lysate was immunoblotted for pCHPKwt (Flag), pUS10 (HA), VP5 (non-specific control), and β-actin (protein loading control). Total lysate was immunoprecipitated with anti-Flag Fab or anti-HA combined with protein A/G-beads, electrophoresed on an SDS-PAGE gel, and then immunoblotted using anti-Flag, -HA, or VP5 antibodies.

    Journal: bioRxiv

    Article Title: The alphaherpesvirus conserved pUS10 is important for natural infection and its expression is regulated by the conserved Herpesviridae protein kinase (CHPK)

    doi: 10.1101/2022.10.31.514468

    Figure Lengend Snippet: Replication of rMDVs in tissue culture cells and expression of pCHPK and pUS10. (A) Plaque areas were measured for rMDVs reconstituted from each parental virus (vCHPKwt, vCHPKmut, vΔCHPK) and HA-tagged US10 virus (vCHPKwt/10HA, vCHPKmut/10HA, vΔCHPK/10HA). There were no significant differences between all groups using One-way ANOVA ( P = 0.71, n = 300) and Levene’s Test ( P = 0.84, n = 300). (B) Representative plaques induced by each virus stained with anti-MDV, -Flag, and -HA antibodies. Included in each image is Hoechst 33342 staining of nuclei for reference. (C) Confocal microscopy of vCHPKwt/10HA-, vCHPKmut/10HA-, or vΔCHPK/10HA-infected CECS. Cells were stained for Flag (CHPK) and HA (US10). (D) Immunoblotting of total protein collected from mock-infected CECs or CECs infected with vCHPKwt/10HA, vCHPKmut/10HA, or vΔCHPK/10HA. To examine CHPK and US10 expression, proteins were electrophoresed under non-reducing conditions and blotted with anti-Flag (CHPK) and -HA (US10) antibodies. Protein was also run under reducing conditions (*) and blotted with anti-β-actin and -MDV pp38 as protein loading and infection level controls, respectively. (E) Confocal images of CECs infected with vCHPKwt/10HA were used to quantify the colocalization of pCHPK and pUS10. A representative image of a plaque stained with anti-Flag (pCHPKwt) and -HA (pUS10) and merged. Hoechst was used to identify cell nuclei. A single z-section with linear ROIs labeled and the profile plot for ROI-1, and its Pearson Correlation Coefficient R is shown. The average Pearson Correlation Coefficient R between pCHPKwt and pUS10 was 0.8859 ± 0.0638. (F) Uninfected CECs (Mock) or CECs infected with vCHPKwt/10HA were used for protein extraction. Total cell lysate was immunoblotted for pCHPKwt (Flag), pUS10 (HA), VP5 (non-specific control), and β-actin (protein loading control). Total lysate was immunoprecipitated with anti-Flag Fab or anti-HA combined with protein A/G-beads, electrophoresed on an SDS-PAGE gel, and then immunoblotted using anti-Flag, -HA, or VP5 antibodies.

    Article Snippet: Anti-HA treated samples were then incubated with a pre-washed 50 μl slurry of protein A/G agarose beads (MilliporeSigma) for 2 h at 4°C.

    Techniques: Expressing, Staining, Confocal Microscopy, Infection, Labeling, Protein Extraction, Immunoprecipitation, SDS Page