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protein 5 fabp5 kits  (Cusabio)


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    Structured Review

    Cusabio protein 5 fabp5 kits
    The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups
    Protein 5 Fabp5 Kits, supplied by Cusabio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein 5 fabp5 kits/product/Cusabio
    Average 86 stars, based on 1 article reviews
    protein 5 fabp5 kits - by Bioz Stars, 2025-02
    86/100 stars

    Images

    1) Product Images from "Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology"

    Article Title: Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

    Journal: Journal of Inflammation Research

    doi: 10.2147/JIR.S444280

    The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups
    Figure Legend Snippet: The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups

    Techniques Used:

    Validation of the top 5 up and down-regulated proteins in an independent validation cohort. ( A – E ) comparison of serum MRC1, CDH13, LTA4H, CD5L and MMP2 concentrations between the eCRSwNP and neCRSwNP groups. ( F – J ) comparison of serum SERPING1, IGFBP5, TRIM28, CHL1 and FABP5 levels between the eCRSwNP and neCRSwNP groups. *P<0.05; **P<0.01; ***P<0.001.
    Figure Legend Snippet: Validation of the top 5 up and down-regulated proteins in an independent validation cohort. ( A – E ) comparison of serum MRC1, CDH13, LTA4H, CD5L and MMP2 concentrations between the eCRSwNP and neCRSwNP groups. ( F – J ) comparison of serum SERPING1, IGFBP5, TRIM28, CHL1 and FABP5 levels between the eCRSwNP and neCRSwNP groups. *P<0.05; **P<0.01; ***P<0.001.

    Techniques Used: Comparison



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    Elevated expression of <t>FABP5</t> induce EMT inbreast cancer cells. a Expression of FABP5 in different mammary epithelial cell lines (left panel) and in non-co-cultivated (NC) and co-cultivated (Coc) breast cancer cells (SUM159PT and SK-BR-3, right panel). b Detection of E-cadherin in the SK-BR-3 breast cancer cells by immunofluorescence analysis (upper panel) and some molecules that may contribute to the enhance malignancy of breast cancers by western blot (lower panel). c Representative immunohistochemical staining of FABP5 in normal breast and tumor tissues
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    Image Search Results


    The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups

    Journal: Journal of Inflammation Research

    Article Title: Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

    doi: 10.2147/JIR.S444280

    Figure Lengend Snippet: The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups

    Article Snippet: Mannose receptor C-type 1 (MRC1) kits (Cat: CSB-E09961h), cadherin 13 (CDH13) kits (Cat: CSB-E13817h), cluster of differentiation 5 antigen-like (CD5L) kits (Cat: CSB-E13423h), matrix metalloproteinase-2 (MMP2) kits (Cat: CSB-E04675h), plasma protease C1 inhibitor (SERPING1) kits (Cat: CSB-EL021086HU), insulin-like growth factor-binding protein 5 (IGFBP5) kits (Cat: CSB-EL010901HU), tripartite motif-containing protein 28 (TRIM28) kits (Cat: CSB-EL024502HU), and fatty acid-binding protein 5 (FABP5) kits (Cat: CSB-EL007946HU) were purchased from Cusabio (Wuhan, China).

    Techniques:

    Validation of the top 5 up and down-regulated proteins in an independent validation cohort. ( A – E ) comparison of serum MRC1, CDH13, LTA4H, CD5L and MMP2 concentrations between the eCRSwNP and neCRSwNP groups. ( F – J ) comparison of serum SERPING1, IGFBP5, TRIM28, CHL1 and FABP5 levels between the eCRSwNP and neCRSwNP groups. *P<0.05; **P<0.01; ***P<0.001.

    Journal: Journal of Inflammation Research

    Article Title: Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

    doi: 10.2147/JIR.S444280

    Figure Lengend Snippet: Validation of the top 5 up and down-regulated proteins in an independent validation cohort. ( A – E ) comparison of serum MRC1, CDH13, LTA4H, CD5L and MMP2 concentrations between the eCRSwNP and neCRSwNP groups. ( F – J ) comparison of serum SERPING1, IGFBP5, TRIM28, CHL1 and FABP5 levels between the eCRSwNP and neCRSwNP groups. *P<0.05; **P<0.01; ***P<0.001.

    Article Snippet: Mannose receptor C-type 1 (MRC1) kits (Cat: CSB-E09961h), cadherin 13 (CDH13) kits (Cat: CSB-E13817h), cluster of differentiation 5 antigen-like (CD5L) kits (Cat: CSB-E13423h), matrix metalloproteinase-2 (MMP2) kits (Cat: CSB-E04675h), plasma protease C1 inhibitor (SERPING1) kits (Cat: CSB-EL021086HU), insulin-like growth factor-binding protein 5 (IGFBP5) kits (Cat: CSB-EL010901HU), tripartite motif-containing protein 28 (TRIM28) kits (Cat: CSB-EL024502HU), and fatty acid-binding protein 5 (FABP5) kits (Cat: CSB-EL007946HU) were purchased from Cusabio (Wuhan, China).

    Techniques: Comparison

    Comparison of E-FABP concentrations in tears and saliva between the wild-type (WT) mice and the NOD mice. ( A ) Tear E-FABP concentration in the NOD mice was significantly lower than in the WT mice. ( p = 0.0135). ( B ) Saliva E-FABP concentration in the NOD mice was also significantly lower than in the WT mice. ( p = 0.04). * represents p < 0.05. E-FABP: epidermal fatty acid binding protein.

    Journal: International Journal of Molecular Sciences

    Article Title: Salivary and Lacrimal Gland Alterations of the Epidermal Fatty Acid-Binding Protein (E-FABP) in Non-Obese Diabetic Mice

    doi: 10.3390/ijms23073491

    Figure Lengend Snippet: Comparison of E-FABP concentrations in tears and saliva between the wild-type (WT) mice and the NOD mice. ( A ) Tear E-FABP concentration in the NOD mice was significantly lower than in the WT mice. ( p = 0.0135). ( B ) Saliva E-FABP concentration in the NOD mice was also significantly lower than in the WT mice. ( p = 0.04). * represents p < 0.05. E-FABP: epidermal fatty acid binding protein.

    Article Snippet: The E-FABP concentration in the resulting supernatants was measured using the FABP5 ELISA Kit (Human) (Aviva Systems Biology, San Diego, CA, USA) based on the standard sandwich ELISA technique and following the manufacturer’s instructions.

    Techniques: Concentration Assay, Binding Assay

    Comparison of E-FABP immunohistochemistry in the salivary gland between the wild-type (WT) mice and the NOD mice. Representative immunohistochemistry images showed less intense immunostaining in the acinar epithelium of the NOD mice ( B ) when compared with the WT mice ( A ) (yellow arrows). IHC: immunohistochemistry.

    Journal: International Journal of Molecular Sciences

    Article Title: Salivary and Lacrimal Gland Alterations of the Epidermal Fatty Acid-Binding Protein (E-FABP) in Non-Obese Diabetic Mice

    doi: 10.3390/ijms23073491

    Figure Lengend Snippet: Comparison of E-FABP immunohistochemistry in the salivary gland between the wild-type (WT) mice and the NOD mice. Representative immunohistochemistry images showed less intense immunostaining in the acinar epithelium of the NOD mice ( B ) when compared with the WT mice ( A ) (yellow arrows). IHC: immunohistochemistry.

    Article Snippet: The E-FABP concentration in the resulting supernatants was measured using the FABP5 ELISA Kit (Human) (Aviva Systems Biology, San Diego, CA, USA) based on the standard sandwich ELISA technique and following the manufacturer’s instructions.

    Techniques: Immunohistochemistry, Immunostaining

    Comparison of E-FABP immunohistochemistry in the lacrimal glands of the wild-type (WT) mice and the NOD mice. Representative immunohistochemistry images showed increased immunostaining of the acinar epithelium in the NOD mice ( B , D ) compared to the WT mice ( A , C ) (yellow arrows). Moreover, a denser infiltration of inflammatory cells around the acinar units of the NOD mice compared to the WT mice can be observed (green arrow). IHC: immunohistochemistry.

    Journal: International Journal of Molecular Sciences

    Article Title: Salivary and Lacrimal Gland Alterations of the Epidermal Fatty Acid-Binding Protein (E-FABP) in Non-Obese Diabetic Mice

    doi: 10.3390/ijms23073491

    Figure Lengend Snippet: Comparison of E-FABP immunohistochemistry in the lacrimal glands of the wild-type (WT) mice and the NOD mice. Representative immunohistochemistry images showed increased immunostaining of the acinar epithelium in the NOD mice ( B , D ) compared to the WT mice ( A , C ) (yellow arrows). Moreover, a denser infiltration of inflammatory cells around the acinar units of the NOD mice compared to the WT mice can be observed (green arrow). IHC: immunohistochemistry.

    Article Snippet: The E-FABP concentration in the resulting supernatants was measured using the FABP5 ELISA Kit (Human) (Aviva Systems Biology, San Diego, CA, USA) based on the standard sandwich ELISA technique and following the manufacturer’s instructions.

    Techniques: Immunohistochemistry, Immunostaining

    Comparison of E-FABP mRNA expressions in the salivary and lacrimal glands of the wild-type (WT) and the NOD mice. ( A ) In the salivary glands, the E-FABP mRNA expression in the NOD mice was significantly lower than that in the WT mice ( p = 0.001). ( B ) In the lacrimal glands, the E-FABP mRNA expression in the NOD mice was significantly higher than that in the WT mice ( p = 0.0008). * and ** represent p < 0.05 and p < 0.001, respectively. SG: salivary gland, LG: lacrimal gland, GAPDH: glyceraldehyde phosphate dehydrogenase.

    Journal: International Journal of Molecular Sciences

    Article Title: Salivary and Lacrimal Gland Alterations of the Epidermal Fatty Acid-Binding Protein (E-FABP) in Non-Obese Diabetic Mice

    doi: 10.3390/ijms23073491

    Figure Lengend Snippet: Comparison of E-FABP mRNA expressions in the salivary and lacrimal glands of the wild-type (WT) and the NOD mice. ( A ) In the salivary glands, the E-FABP mRNA expression in the NOD mice was significantly lower than that in the WT mice ( p = 0.001). ( B ) In the lacrimal glands, the E-FABP mRNA expression in the NOD mice was significantly higher than that in the WT mice ( p = 0.0008). * and ** represent p < 0.05 and p < 0.001, respectively. SG: salivary gland, LG: lacrimal gland, GAPDH: glyceraldehyde phosphate dehydrogenase.

    Article Snippet: The E-FABP concentration in the resulting supernatants was measured using the FABP5 ELISA Kit (Human) (Aviva Systems Biology, San Diego, CA, USA) based on the standard sandwich ELISA technique and following the manufacturer’s instructions.

    Techniques: Expressing

    Elevated expression of FABP5 induce EMT inbreast cancer cells. a Expression of FABP5 in different mammary epithelial cell lines (left panel) and in non-co-cultivated (NC) and co-cultivated (Coc) breast cancer cells (SUM159PT and SK-BR-3, right panel). b Detection of E-cadherin in the SK-BR-3 breast cancer cells by immunofluorescence analysis (upper panel) and some molecules that may contribute to the enhance malignancy of breast cancers by western blot (lower panel). c Representative immunohistochemical staining of FABP5 in normal breast and tumor tissues

    Journal: Cell Communication and Signaling : CCS

    Article Title: Utilization of adipocyte-derived lipids and enhanced intracellular trafficking of fatty acids contribute to breast cancer progression

    doi: 10.1186/s12964-018-0221-6

    Figure Lengend Snippet: Elevated expression of FABP5 induce EMT inbreast cancer cells. a Expression of FABP5 in different mammary epithelial cell lines (left panel) and in non-co-cultivated (NC) and co-cultivated (Coc) breast cancer cells (SUM159PT and SK-BR-3, right panel). b Detection of E-cadherin in the SK-BR-3 breast cancer cells by immunofluorescence analysis (upper panel) and some molecules that may contribute to the enhance malignancy of breast cancers by western blot (lower panel). c Representative immunohistochemical staining of FABP5 in normal breast and tumor tissues

    Article Snippet: A fatty acid binding protein 5 (FABP5) siRNA kit was purchased from RIBOBIO (Guangzhou).

    Techniques: Expressing, Immunofluorescence, Western Blot, Immunohistochemical staining, Staining

    Immunohistochemistry staining intensity of  FABP5  in various breast cancer patients

    Journal: Cell Communication and Signaling : CCS

    Article Title: Utilization of adipocyte-derived lipids and enhanced intracellular trafficking of fatty acids contribute to breast cancer progression

    doi: 10.1186/s12964-018-0221-6

    Figure Lengend Snippet: Immunohistochemistry staining intensity of FABP5 in various breast cancer patients

    Article Snippet: A fatty acid binding protein 5 (FABP5) siRNA kit was purchased from RIBOBIO (Guangzhou).

    Techniques: Immunohistochemistry, Staining, Expressing

    In the presence of adipocytes, accelerated FA trafficking in breast cancer cells was mediated by FABP5. a After ablation of FABP5 using an siRNA kit, cells were harvested 48 h after transfection for western blotting to the validate knockdown effectiveness. b Impact on the proliferative ability of breast cancer cell lines resulting from FABP5knockdown. c Altered migration ability resulting from FABP5 deletion in non-cultivated (NC) and co-cultivated (Coc) cancer cells. d TG storage in breast cancer cells, with FABP5 expressed or ablated, cultivated in the absence or presence of adipocytes. * p < 0.05, ** p < 0.01, **** p < 0.0001, “ns” stands for not significant

    Journal: Cell Communication and Signaling : CCS

    Article Title: Utilization of adipocyte-derived lipids and enhanced intracellular trafficking of fatty acids contribute to breast cancer progression

    doi: 10.1186/s12964-018-0221-6

    Figure Lengend Snippet: In the presence of adipocytes, accelerated FA trafficking in breast cancer cells was mediated by FABP5. a After ablation of FABP5 using an siRNA kit, cells were harvested 48 h after transfection for western blotting to the validate knockdown effectiveness. b Impact on the proliferative ability of breast cancer cell lines resulting from FABP5knockdown. c Altered migration ability resulting from FABP5 deletion in non-cultivated (NC) and co-cultivated (Coc) cancer cells. d TG storage in breast cancer cells, with FABP5 expressed or ablated, cultivated in the absence or presence of adipocytes. * p < 0.05, ** p < 0.01, **** p < 0.0001, “ns” stands for not significant

    Article Snippet: A fatty acid binding protein 5 (FABP5) siRNA kit was purchased from RIBOBIO (Guangzhou).

    Techniques: Transfection, Western Blot, Migration

    Exogenous lipids in the absence of adipocytes could not promote the malignant behavior of cancer cells. Mature adipocytes were replaced by 0.02% Intralipid in 3 day co-culture with SUM159PT breast cancer cells. a Bodipy staining was performed on SUM159PT cells grown alone (NC) and in medium supplemented with Intralipid. (lipids are shown in green, and nuclei are shown in blue; scale bar, 50 μm). b The TG content in the two cell populations (NC vs. Intralipid) was measured. c-d Proliferation and migration assays were employed to compare the malignant properties of non-treated cells (NC) and Intralipid-treated cells. e SUM159PT cells were not treated (NC) or treated with Intralipid for 3 days and then released similar to co-culture. Cells were collected at 0, 4 and 24 h for TG measurement, and medium was collected at 4 and 24 h for free glycerol measurement. f Differential expression of lipases, pAMPK/AMPK and FABP5 in breast cancer cells (SUM159PT) grown in Intralipid or with adipocytes. *** p < 0.001, “ns” means not significant

    Journal: Cell Communication and Signaling : CCS

    Article Title: Utilization of adipocyte-derived lipids and enhanced intracellular trafficking of fatty acids contribute to breast cancer progression

    doi: 10.1186/s12964-018-0221-6

    Figure Lengend Snippet: Exogenous lipids in the absence of adipocytes could not promote the malignant behavior of cancer cells. Mature adipocytes were replaced by 0.02% Intralipid in 3 day co-culture with SUM159PT breast cancer cells. a Bodipy staining was performed on SUM159PT cells grown alone (NC) and in medium supplemented with Intralipid. (lipids are shown in green, and nuclei are shown in blue; scale bar, 50 μm). b The TG content in the two cell populations (NC vs. Intralipid) was measured. c-d Proliferation and migration assays were employed to compare the malignant properties of non-treated cells (NC) and Intralipid-treated cells. e SUM159PT cells were not treated (NC) or treated with Intralipid for 3 days and then released similar to co-culture. Cells were collected at 0, 4 and 24 h for TG measurement, and medium was collected at 4 and 24 h for free glycerol measurement. f Differential expression of lipases, pAMPK/AMPK and FABP5 in breast cancer cells (SUM159PT) grown in Intralipid or with adipocytes. *** p < 0.001, “ns” means not significant

    Article Snippet: A fatty acid binding protein 5 (FABP5) siRNA kit was purchased from RIBOBIO (Guangzhou).

    Techniques: Co-Culture Assay, Staining, Migration, Expressing