protease phosphatase inhibitor cocktail  (Cell Signaling Technology Inc)

 
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    Name:
    Protease Phosphatase Inhibitor Cocktail 100X
    Description:
    When diluted in lysis buffer to a final concentration of 1X the Protease Phosphatase Inhibitor Cocktail prevents protein degradation and dephosphorylation by endogenous proteases and phosphatases present in the whole cell extract The 100X cocktail is a clear light yellow to light green liquid
    Catalog Number:
    5872
    Price:
    None
    Category:
    Buffers Dyes
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    Structured Review

    Cell Signaling Technology Inc protease phosphatase inhibitor cocktail
    When diluted in lysis buffer to a final concentration of 1X the Protease Phosphatase Inhibitor Cocktail prevents protein degradation and dephosphorylation by endogenous proteases and phosphatases present in the whole cell extract The 100X cocktail is a clear light yellow to light green liquid
    https://www.bioz.com/result/protease phosphatase inhibitor cocktail/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protease phosphatase inhibitor cocktail - by Bioz Stars, 2021-03
    97/100 stars

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    Related Articles

    other:

    Article Title: In wound repair vimentin mediates the transition of mesenchymal leader cells to a myofibroblast phenotype
    Article Snippet: The reaction was quenched with (50 mM Tris-HCl), and the cells were extracted with RIPA buffer containing protease/phosphatase inhibitor cocktail (Cell Signaling, Danvers, MA) and the substrate-associated proteins fixed with 3.7% formaldehyde in PBS.

    Article Title: A pilot study comparing protein expression in different segments of the normal colon and rectum and in normal colon versus adenoma in patients with Lynch syndrome
    Article Snippet: The tissues were cut into small pieces on dry ice and then homogenized with ice-cold RIPA buffer (Cell Signaling #9806, Boston, MA) containing freshly added protease/phosphatase inhibitors (Cell Signaling #5872).

    Article Title: Interleukin-33 Receptor (ST2) Deficiency Improves the Outcome of Staphylococcus aureus-Induced Septic Arthritis
    Article Snippet: The cell lysates were collected using RIPA buffer containing CST—Protease/Phosphatase Inhibitor Cocktail (Cell Signaling, USA).

    Expressing:

    Article Title: Effects of gut microbiota manipulation on ex vivo lipolysis in human abdominal subcutaneous adipocytes
    Article Snippet: Relevant lipolysis related genes were selected from the total microarray as published in the clinical trail where the current study is part of (GEO GSE76003) [ ]. .. Adipose tissue protein expression Adipose tissue biopsies (100–300 mg) were ground to a fine powder under liquid nitrogen and homogenized in 200–600 µL RIPA buffer supplemented with a protease/phosphatase inhibitor cocktail (Cell Signalling Technology Europe, Leiden, The Netherlands). ..

    Article Title: In wound repair vimentin mediates the transition of mesenchymal leader cells to a myofibroblast phenotype
    Article Snippet: To determine the relative distribution of Triton/OG soluble and insoluble proteins the Triton/OG soluble fractions were loaded at equal protein concentration and the SDS fractions were loaded at volumes equal to the respective Triton/OG soluble fractions. .. Samples were extracted in Triton/OG except for studies of α-SMA expression for which the cells were extracted in RIPA buffer (5 mM EDTA, 150 mM NaCl, 1% NP40, 1% sodium deoxycholate, 1% SDS 20% solution, 50 mM Tris-HCl, pH 7.4) containing protease/phosphatase inhibitor cocktail (Cell Signaling, Danvers, MA). .. All protein concentrations were determined by a BCA protein assay (BCA Assay; Pierce, Rockford, IL).

    Western Blot:

    Article Title: A Universal Approach to Correct Various HBB Gene Mutations in Human Stem Cells for Gene Therapy of Beta‐Thalassemia and Sickle Cell Disease
    Article Snippet: .. Western Blotting The iPSCs and erythrocytes at TM day 8 were lysed with RIPA buffer (Sigma, St. Louis, MO, #R0278) in the presence of protease/phosphatase inhibitor cocktail (Cell Signaling Technology, Danvers, MA, #5872S). .. The protein samples were treated and run with NuPAGE Bis‐Tris Mini Gel System (Life Technologies) following manufacturer's instruction.

    Cell Culture:

    Article Title: Conditioned media from AICAR-treated skeletal muscle cells increases neuronal differentiation of adult neural progenitor cells
    Article Snippet: For real-time RT-PCR, total RNA (100 ng) was amplified using the PerfeCTa SYBR Green FastMix (Quanta Biosciences) and ECO PCR system (Illumina). .. Cell culture and preparation of CM) collected from L6 muscle cells was mixed with 1:1 ratio RIPA buffer (Millipore), Protease/Phosphatase Inhibitor Cocktail (Cell Signaling Technology, Danvers, MA) and sample buffer (Bio-rad). .. For Western blot analysis, containing equal amounts of protein from each fraction were subjected to SDS-PAGE and transferred to a NC membrane (Millipore).

    SDS Page:

    Article Title: Suppression of the GTPase-activating protein RGS10 increases Rheb-GTP and mTOR signaling in ovarian cancer cells
    Article Snippet: .. The cells were lysed in buffer containing protease/phosphatase inhibitor cocktail (Cell Signaling Technology, Danvers, MA) and processed for SDS-PAGE. .. After transferring the denatured proteins to nitrocellulose membranes, the blots were probed with primary antibodies for either total mTOR, phospho-mTOR (Ser2448) XP Rabbit mAb #5536, total 4E-BP-1, phospho-4E-BP1 (Thr37/46) #2855, GAPDH, β-Actin (8H10D10) Mouse mAb #3700, phospho-Akt (Ser473) XP Rabbit mAb #4060, phospho-p70 S6 kinase (Thr389) Rabbit mAb #9234, phospho-p70 S6 kinase (Ser371) antibody #9208, phospho-S6 ribosomal protein (Ser235/236) Rabbit mAb #4858, and phospho-eIF2α (Ser51) XP Rabbit mAb #3398 (all from Cell Signaling Technology) or RGS10 (Santa Cruz Biotechnology, Dallas, TX).

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    Cell Signaling Technology Inc cst protease phosphatase inhibitor cocktail
    Cst Protease Phosphatase Inhibitor Cocktail, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc protease phosphatase inhibitor cocktail
    Protease Phosphatase Inhibitor Cocktail, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease phosphatase inhibitor cocktail/product/Cell Signaling Technology Inc
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    cell signaling technology inc phosphatase inhibitors cocktail
    Phosphatase Inhibitors Cocktail, supplied by cell signaling technology inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Cell Signaling Technology Inc buffer
    Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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