radioimmune precipitation assay buffer  (Roche)


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    Structured Review

    Roche radioimmune precipitation assay buffer
    Radioimmune Precipitation Assay Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/radioimmune precipitation assay buffer/product/Roche
    Average 93 stars, based on 131 article reviews
    Price from $9.99 to $1999.99
    radioimmune precipitation assay buffer - by Bioz Stars, 2020-09
    93/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Hepatitis C Virus JFH-1 Strain Infection in Chimpanzees Is Associated with Low Pathogenicity and Emergence of an Adaptive Mutation
    Article Snippet: .. To determine intracellular HCV core Ag, the cell pellet was re-suspended with 100 μl of radioimmune precipitation assay buffer containing 1% sodium dodecyl sulfate, 0.5% NP40, 10 mM Tris-HCl (pH 7.4), 1 mM EDTA, 150 mM NaCl and Complete Mini protease inhibitor cocktail (Roche Applied Science, Indianapolis, IN) then sonicated 10 minutes and subjected to the Ortho HCV core antigen ELISA assay following centrifugation. .. To assess the intracellular infectivity, cells were harvested by treatment with trypsin-EDTA and pelleted by centrifugation.

    Article Title: Protein Kinase C? Supports Survival of MDA-MB-231 Breast Cancer Cells by Suppressing the ERK1/2 Pathway *
    Article Snippet: .. Cells were lysed using radioimmune precipitation assay buffer (10 m m Tris-HCl (pH 7.2), 160 m m NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, 1 m m EDTA, and 1 m m EGTA) containing 40 μl/ml Complete protease inhibitor (Roche Applied Science) and incubated on ice for 30 min. Lysates were cleared by centrifugation at 14,000 × g for 10 min at 4 °C, diluted in sample buffer containing β-mercaptoethanol, and boiled for 5 min. Proteins were electrophoretically separated on 10% NuPAGE Novex BisTris gels (Invitrogen) and transferred to polyvinylidene difluoride membranes (Millipore). .. Membranes were blocked with phosphate-buffered saline containing 5% nonfat milk and probed with antibodies to PKCδ (1:500), PKCϵ (1:500), and PKCα (1:3000) (Santa Cruz Biotechnology); caspase-3, phospho-ERK1/2, ERK1/2, phospho-MEK1/2, MEK1/2, phospho-Akt, and Akt (all at 1:500; Cell Signaling); Nedd4 (1:500; Millipore); SMURF2 (1:1000; Upstate); MKP3 (2 μg/μl) ( ); and actin (1:2000; MP Biomedicals).

    Protease Inhibitor:

    Article Title: Propofol inhibits stromatoxin-1-sensitive voltage-dependent K+ channels in pancreatic β-cells and enhances insulin secretion
    Article Snippet: .. Immunoblotting Whole-cell lysates were prepared by incubating cells for 30 min in cold radioimmune precipitation assay buffer with Complete Protease Inhibitor Cocktail Tablets (Roche Diagnostics, Tokyo, Japan). .. Samples were then centrifuged at 10,000× g to allow the cell debris to settle, and 30 µg total protein from the resulting supernatant was separated by 7.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electro-transferred onto membranes, probed with 1:500 (anti-Kv2.1 Ab) or 1:1000 (anti- β-actin Ab) of the indicated primary antibodies, and then probed with 1:8,000 of donkey anti-rabbit IgG (GE Healthcare, Piscataway, NJ) or sheep anti-mouse IgG (GE Healthcare) conjugated with horseradish peroxidase.

    Article Title: Activation of Gene Transcription by Heat Shock Protein 27 May Contribute to Its Neuronal Protection *
    Article Snippet: .. For whole cell lysates, cells were washed with 1× phosphate-buffered saline and harvested in radioimmune precipitation assay buffer (50 m m Tris-HCl (pH 8.0), 150 m m NaCl, 1 m m EDTA (pH 8.0), 1 m m EGTA (pH 8.0), 0.1% SDS, 0.5% sodium deoxycholate, and 1% Triton X-100) containing protease inhibitor mixture (Roche Applied Science). .. When relevant, HALT phosphatase inhibitor mixture (Pierce) was included in the lysis buffer.

    Article Title: Hepatitis C Virus JFH-1 Strain Infection in Chimpanzees Is Associated with Low Pathogenicity and Emergence of an Adaptive Mutation
    Article Snippet: .. To determine intracellular HCV core Ag, the cell pellet was re-suspended with 100 μl of radioimmune precipitation assay buffer containing 1% sodium dodecyl sulfate, 0.5% NP40, 10 mM Tris-HCl (pH 7.4), 1 mM EDTA, 150 mM NaCl and Complete Mini protease inhibitor cocktail (Roche Applied Science, Indianapolis, IN) then sonicated 10 minutes and subjected to the Ortho HCV core antigen ELISA assay following centrifugation. .. To assess the intracellular infectivity, cells were harvested by treatment with trypsin-EDTA and pelleted by centrifugation.

    Article Title: Exogenous delivery of chaperonin subunit fragment ApiCCT1 modulates mutant Huntingtin cellular phenotypes
    Article Snippet: .. At 48 h postinduction, cells were lysed in radioimmune precipitation assay buffer [10 mM Tris, pH 7.5, 150 mM NaCl, 1 mM EDTA (pH 8.0), 1% Nonidet P-40, 0.5% SDS] containing Complete Protease Inhibitor (Roche Diagnostics). ..

    Article Title: Identification of Epithelial to Mesenchymal Transition as a Novel Source of Fibroblasts in Intestinal Fibrosis *
    Article Snippet: .. Total cell culture and tissue lysates were made using radioimmune precipitation assay buffer plus a protease inhibitor mixture (Roche Applied Science). .. Antibodies to β-actin and E-cadherin were purchased from Sigma and BD Biosciences, respectively.

    Article Title: Protein Kinase C? Supports Survival of MDA-MB-231 Breast Cancer Cells by Suppressing the ERK1/2 Pathway *
    Article Snippet: .. Cells were lysed using radioimmune precipitation assay buffer (10 m m Tris-HCl (pH 7.2), 160 m m NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, 1 m m EDTA, and 1 m m EGTA) containing 40 μl/ml Complete protease inhibitor (Roche Applied Science) and incubated on ice for 30 min. Lysates were cleared by centrifugation at 14,000 × g for 10 min at 4 °C, diluted in sample buffer containing β-mercaptoethanol, and boiled for 5 min. Proteins were electrophoretically separated on 10% NuPAGE Novex BisTris gels (Invitrogen) and transferred to polyvinylidene difluoride membranes (Millipore). .. Membranes were blocked with phosphate-buffered saline containing 5% nonfat milk and probed with antibodies to PKCδ (1:500), PKCϵ (1:500), and PKCα (1:3000) (Santa Cruz Biotechnology); caspase-3, phospho-ERK1/2, ERK1/2, phospho-MEK1/2, MEK1/2, phospho-Akt, and Akt (all at 1:500; Cell Signaling); Nedd4 (1:500; Millipore); SMURF2 (1:1000; Upstate); MKP3 (2 μg/μl) ( ); and actin (1:2000; MP Biomedicals).

    Article Title: Thrombospondin-1 Inhibits VEGF Receptor-2 Signaling by Disrupting Its Association with CD47
    Article Snippet: .. Cell lysates were made using either radioimmune precipitation assay buffer (50 m m Tris (pH 7.4), 150 m m NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 1 m m EGTA, and 1 m m NaF) or immunoprecipitation buffer (50 m m Tris-HCl, 150 m m NaCl, and 1% Nonidet P-40) along with 1× Complete Mini protease inhibitor mixture (Roche Applied Science) and Na3 VO4 . .. Cell lysates were centrifuged at 13,000 rpm for 15 min. A BCA assay (Pierce) was used to quantify total protein.

    Cell Culture:

    Article Title: Identification of Epithelial to Mesenchymal Transition as a Novel Source of Fibroblasts in Intestinal Fibrosis *
    Article Snippet: .. Total cell culture and tissue lysates were made using radioimmune precipitation assay buffer plus a protease inhibitor mixture (Roche Applied Science). .. Antibodies to β-actin and E-cadherin were purchased from Sigma and BD Biosciences, respectively.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Hepatitis C Virus JFH-1 Strain Infection in Chimpanzees Is Associated with Low Pathogenicity and Emergence of an Adaptive Mutation
    Article Snippet: .. To determine intracellular HCV core Ag, the cell pellet was re-suspended with 100 μl of radioimmune precipitation assay buffer containing 1% sodium dodecyl sulfate, 0.5% NP40, 10 mM Tris-HCl (pH 7.4), 1 mM EDTA, 150 mM NaCl and Complete Mini protease inhibitor cocktail (Roche Applied Science, Indianapolis, IN) then sonicated 10 minutes and subjected to the Ortho HCV core antigen ELISA assay following centrifugation. .. To assess the intracellular infectivity, cells were harvested by treatment with trypsin-EDTA and pelleted by centrifugation.

    Immunoprecipitation:

    Article Title: Thrombospondin-1 Inhibits VEGF Receptor-2 Signaling by Disrupting Its Association with CD47
    Article Snippet: .. Cell lysates were made using either radioimmune precipitation assay buffer (50 m m Tris (pH 7.4), 150 m m NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 1 m m EGTA, and 1 m m NaF) or immunoprecipitation buffer (50 m m Tris-HCl, 150 m m NaCl, and 1% Nonidet P-40) along with 1× Complete Mini protease inhibitor mixture (Roche Applied Science) and Na3 VO4 . .. Cell lysates were centrifuged at 13,000 rpm for 15 min. A BCA assay (Pierce) was used to quantify total protein.

    Incubation:

    Article Title: Protein Kinase C? Supports Survival of MDA-MB-231 Breast Cancer Cells by Suppressing the ERK1/2 Pathway *
    Article Snippet: .. Cells were lysed using radioimmune precipitation assay buffer (10 m m Tris-HCl (pH 7.2), 160 m m NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, 1 m m EDTA, and 1 m m EGTA) containing 40 μl/ml Complete protease inhibitor (Roche Applied Science) and incubated on ice for 30 min. Lysates were cleared by centrifugation at 14,000 × g for 10 min at 4 °C, diluted in sample buffer containing β-mercaptoethanol, and boiled for 5 min. Proteins were electrophoretically separated on 10% NuPAGE Novex BisTris gels (Invitrogen) and transferred to polyvinylidene difluoride membranes (Millipore). .. Membranes were blocked with phosphate-buffered saline containing 5% nonfat milk and probed with antibodies to PKCδ (1:500), PKCϵ (1:500), and PKCα (1:3000) (Santa Cruz Biotechnology); caspase-3, phospho-ERK1/2, ERK1/2, phospho-MEK1/2, MEK1/2, phospho-Akt, and Akt (all at 1:500; Cell Signaling); Nedd4 (1:500; Millipore); SMURF2 (1:1000; Upstate); MKP3 (2 μg/μl) ( ); and actin (1:2000; MP Biomedicals).

    Modification:

    Article Title: Tristetraprolin posttranscriptionally downregulates PFKFB3 in cancer cells.
    Article Snippet: .. The enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases 3 (PFKFB3) catalyzes the first committed rate-limiting step of glycolysis and is upregulated in cancer cells. .. The enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases 3 (PFKFB3) catalyzes the first committed rate-limiting step of glycolysis and is upregulated in cancer cells.

    Sonication:

    Article Title: Tristetraprolin posttranscriptionally downregulates PFKFB3 in cancer cells.
    Article Snippet: .. The enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases 3 (PFKFB3) catalyzes the first committed rate-limiting step of glycolysis and is upregulated in cancer cells. .. The enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases 3 (PFKFB3) catalyzes the first committed rate-limiting step of glycolysis and is upregulated in cancer cells.

    Article Title: Hepatitis C Virus JFH-1 Strain Infection in Chimpanzees Is Associated with Low Pathogenicity and Emergence of an Adaptive Mutation
    Article Snippet: .. To determine intracellular HCV core Ag, the cell pellet was re-suspended with 100 μl of radioimmune precipitation assay buffer containing 1% sodium dodecyl sulfate, 0.5% NP40, 10 mM Tris-HCl (pH 7.4), 1 mM EDTA, 150 mM NaCl and Complete Mini protease inhibitor cocktail (Roche Applied Science, Indianapolis, IN) then sonicated 10 minutes and subjected to the Ortho HCV core antigen ELISA assay following centrifugation. .. To assess the intracellular infectivity, cells were harvested by treatment with trypsin-EDTA and pelleted by centrifugation.

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    Roche protease inhibitors ppi 5 m m naf 5 m m β glycerophosphate 1 tablet inhibitor mixture
    Protease Inhibitors Ppi 5 M M Naf 5 M M β Glycerophosphate 1 Tablet Inhibitor Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitors ppi 5 m m naf 5 m m β glycerophosphate 1 tablet inhibitor mixture/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protease inhibitors ppi 5 m m naf 5 m m β glycerophosphate 1 tablet inhibitor mixture - by Bioz Stars, 2020-09
    85/100 stars
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