protease inhibitor phenylmethanesulfonyl fluoride  (Amresco)

 
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    Amresco protease inhibitor phenylmethanesulfonyl fluoride
    Protease Inhibitor Phenylmethanesulfonyl Fluoride, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor phenylmethanesulfonyl fluoride/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor phenylmethanesulfonyl fluoride - by Bioz Stars, 2021-04
    86/100 stars

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    Related Articles

    Lysis:

    Article Title: Amyloid-β with isomerized Asp7 cytotoxicity is coupled to protein phosphorylation
    Article Snippet: The cell pellets were resuspended in HBSS and 2.0 × 106 cells were labelled with 1.85 MBq of L-[35 S]methionine (Amersham Biosciences Corp., Piscataway, NJ, USA) in HBSS for 1 h at 37 °C. .. The 2 × 106 L-[35 S]methionine-labelled and unlabelled cells were lysed by stirring at 4 °C for 15 min in 70 µl of O’Farrell lysis buffer with 1% PMSF and 1% protease-inhibitor cocktail (Amresco). .. Halt Phosphatase Inhibitor Cocktail (Thermo scientific) was added into the samples for staining with pro-Q diamond phosphoprotein gel stain.The probes were then centrifuged at 15,000 g for 10 min, and the supernatant was collected.

    Article Title: Inhibition of cell migration by focal adhesion kinase: Time-dependent difference in integrin-induced signaling between endothelial and hepatoblastoma cells
    Article Snippet: Briefly, following treatment with the FAK inhibitor for 0, 5, 10, 30, 60 and 120 min, EA.hy926 and HepG2 cells were washed three times with PBS and lysed by 200 µ l cell lysis solution (Beyotime Institute of Biotechnology). .. The RIPA lysis buffer includes phosphatase inhibitor (Na3 VO4 ), protease inhibitor mixtures (BestBio Biotechnology Co., Ltd., Shanghai, China) and phenylmethanesulfonyl fluoride (PMSF; Amresco, LLC, Solon, OH, USA), at a ratio of 100 (RIPA):1 (phosphatase inhibitor):1 (protease inhibitor mixtures):1 (PMSF). ..

    Article Title: Comparative proteomic analysis of silica-induced pulmonary fibrosis in rats based on tandem mass tag (TMT) quantitation technology
    Article Snippet: .. Briefly, 300 μL lysis buffer SDS (P0013G, Beyotime Biotechnology, China) and 1 mM protease inhibitor Phenylmethanesulfonyl fluoride (PMSF, PB0425-5G, Amresco, USA) were added to the frozen sample, followed by ultrasonic treatment (1 s/1 s intervals, 80 W) on ice for 3 min and centrifugation (12,000 g, 4°C) for 10 min. .. The protein concentrations were assayed by the method of BCA (23227, Thermo, USA) according to the manufacturer’s instructions.

    Article Title: Systematic revelation of the protective effect and mechanism of Cordycep sinensis on diethylnitrosamine-induced rat hepatocellular carcinoma with proteomics
    Article Snippet: Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) The protein extraction was prepared as follows. .. Small piece of frozen liver (∼ 20 mg) was solubilized in lysis buffer containing 7 M urea, 2 M thiourea, 4% CHAPS, 2% IPG buffer, 65 mM DTT, 10 mM PMSF and protease inhibitor cocktail (AMRESCO, Solon, OH, USA) and subjected to homogenize for 60 seconds on ice. .. The lysate was centrifuged at 10,000 rpm (Kubota 3500, Japan) at 4°C for 20 min to remove insoluble material.

    Protease Inhibitor:

    Article Title: Amyloid-β with isomerized Asp7 cytotoxicity is coupled to protein phosphorylation
    Article Snippet: The cell pellets were resuspended in HBSS and 2.0 × 106 cells were labelled with 1.85 MBq of L-[35 S]methionine (Amersham Biosciences Corp., Piscataway, NJ, USA) in HBSS for 1 h at 37 °C. .. The 2 × 106 L-[35 S]methionine-labelled and unlabelled cells were lysed by stirring at 4 °C for 15 min in 70 µl of O’Farrell lysis buffer with 1% PMSF and 1% protease-inhibitor cocktail (Amresco). .. Halt Phosphatase Inhibitor Cocktail (Thermo scientific) was added into the samples for staining with pro-Q diamond phosphoprotein gel stain.The probes were then centrifuged at 15,000 g for 10 min, and the supernatant was collected.

    Article Title: Inhibition of cell migration by focal adhesion kinase: Time-dependent difference in integrin-induced signaling between endothelial and hepatoblastoma cells
    Article Snippet: Briefly, following treatment with the FAK inhibitor for 0, 5, 10, 30, 60 and 120 min, EA.hy926 and HepG2 cells were washed three times with PBS and lysed by 200 µ l cell lysis solution (Beyotime Institute of Biotechnology). .. The RIPA lysis buffer includes phosphatase inhibitor (Na3 VO4 ), protease inhibitor mixtures (BestBio Biotechnology Co., Ltd., Shanghai, China) and phenylmethanesulfonyl fluoride (PMSF; Amresco, LLC, Solon, OH, USA), at a ratio of 100 (RIPA):1 (phosphatase inhibitor):1 (protease inhibitor mixtures):1 (PMSF). ..

    Article Title: Generation of Knockout and Transgenic Zebrafish to Characterize Abcc4 Functions in Detoxification and Efflux of Lead
    Article Snippet: The protein extraction from embryonic cells was performed following our previous protocol [ ]. .. The adult tissues were homogenized with liquid nitrogen and lysed in a radio-immunoprecipitation assay (RIPA) buffer with phenylmethanesulfonyl fluoride (a protease inhibitor; Amresco, OH, USA). .. The protein concentrations were measured using the BCA method (Beyotime, Nantong, China).

    Article Title: Comparative proteomic analysis of silica-induced pulmonary fibrosis in rats based on tandem mass tag (TMT) quantitation technology
    Article Snippet: .. Briefly, 300 μL lysis buffer SDS (P0013G, Beyotime Biotechnology, China) and 1 mM protease inhibitor Phenylmethanesulfonyl fluoride (PMSF, PB0425-5G, Amresco, USA) were added to the frozen sample, followed by ultrasonic treatment (1 s/1 s intervals, 80 W) on ice for 3 min and centrifugation (12,000 g, 4°C) for 10 min. .. The protein concentrations were assayed by the method of BCA (23227, Thermo, USA) according to the manufacturer’s instructions.

    Article Title: Portulacerebroside A inhibits adhesion, migration, and invasion of human leukemia HL60 cells and U937 cells through the regulation of p38/JNK signaling pathway
    Article Snippet: Migration rate = Migrated cell number in treated group Migrated cell number in control group × 100 % .. Western blot Cells were collected at a density of 5×105 cells/well in 6-well plates after treatment with PCA (1, 2, and 5 μM) for 24 h. Each group of cells was harvested and washed twice with PBS and protein lysed in ice-cold radio immunoprecipitation assay buffer (RIPA; Beyotime, Shanghai, People’s Republic of China) with freshly added 0.01% protease inhibitor phenylmethanesulfonyl fluoride (Amresco, Shanghai, People’s Republic of China) and incubated on ice for 30 min. .. Cell lysis was centrifuged at 10,000× g for 5 min at 4°C, and the supernatant (20–30 μg of protein) was run on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis gel and transferred electrophoretically to a nitrocellulose membrane (Millipore, Shanghai, People’s Republic of China), then detected with JNK, phosphorylated (p-) JNK, P38, p-P38, RhoC, MTA1, and MMP-2/9 proteins.

    Article Title: Inhibition of Proteasome Activity Induces Aggregation of IFIT2 in the Centrosome and Enhances IFIT2-Induced Cell Apoptosis
    Article Snippet: Colchicine (Sigma) was dissolved in the phosphate-buffered saline (PBS) as a stock solution at 100 μg/mL. .. Protease inhibitor phenylmethanesulfonyl fluoride (PMSF, AMRESCO, OH, USA) and cocktail (Roche, Switzerland) were dissolved in isopropanol and PBS as a stock solution, respectively. ..

    Article Title: Systematic revelation of the protective effect and mechanism of Cordycep sinensis on diethylnitrosamine-induced rat hepatocellular carcinoma with proteomics
    Article Snippet: Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) The protein extraction was prepared as follows. .. Small piece of frozen liver (∼ 20 mg) was solubilized in lysis buffer containing 7 M urea, 2 M thiourea, 4% CHAPS, 2% IPG buffer, 65 mM DTT, 10 mM PMSF and protease inhibitor cocktail (AMRESCO, Solon, OH, USA) and subjected to homogenize for 60 seconds on ice. .. The lysate was centrifuged at 10,000 rpm (Kubota 3500, Japan) at 4°C for 20 min to remove insoluble material.

    Radio Immunoprecipitation:

    Article Title: Generation of Knockout and Transgenic Zebrafish to Characterize Abcc4 Functions in Detoxification and Efflux of Lead
    Article Snippet: The protein extraction from embryonic cells was performed following our previous protocol [ ]. .. The adult tissues were homogenized with liquid nitrogen and lysed in a radio-immunoprecipitation assay (RIPA) buffer with phenylmethanesulfonyl fluoride (a protease inhibitor; Amresco, OH, USA). .. The protein concentrations were measured using the BCA method (Beyotime, Nantong, China).

    Article Title: Portulacerebroside A inhibits adhesion, migration, and invasion of human leukemia HL60 cells and U937 cells through the regulation of p38/JNK signaling pathway
    Article Snippet: Migration rate = Migrated cell number in treated group Migrated cell number in control group × 100 % .. Western blot Cells were collected at a density of 5×105 cells/well in 6-well plates after treatment with PCA (1, 2, and 5 μM) for 24 h. Each group of cells was harvested and washed twice with PBS and protein lysed in ice-cold radio immunoprecipitation assay buffer (RIPA; Beyotime, Shanghai, People’s Republic of China) with freshly added 0.01% protease inhibitor phenylmethanesulfonyl fluoride (Amresco, Shanghai, People’s Republic of China) and incubated on ice for 30 min. .. Cell lysis was centrifuged at 10,000× g for 5 min at 4°C, and the supernatant (20–30 μg of protein) was run on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis gel and transferred electrophoretically to a nitrocellulose membrane (Millipore, Shanghai, People’s Republic of China), then detected with JNK, phosphorylated (p-) JNK, P38, p-P38, RhoC, MTA1, and MMP-2/9 proteins.

    Centrifugation:

    Article Title: Comparative proteomic analysis of silica-induced pulmonary fibrosis in rats based on tandem mass tag (TMT) quantitation technology
    Article Snippet: .. Briefly, 300 μL lysis buffer SDS (P0013G, Beyotime Biotechnology, China) and 1 mM protease inhibitor Phenylmethanesulfonyl fluoride (PMSF, PB0425-5G, Amresco, USA) were added to the frozen sample, followed by ultrasonic treatment (1 s/1 s intervals, 80 W) on ice for 3 min and centrifugation (12,000 g, 4°C) for 10 min. .. The protein concentrations were assayed by the method of BCA (23227, Thermo, USA) according to the manufacturer’s instructions.

    Western Blot:

    Article Title: Portulacerebroside A inhibits adhesion, migration, and invasion of human leukemia HL60 cells and U937 cells through the regulation of p38/JNK signaling pathway
    Article Snippet: Migration rate = Migrated cell number in treated group Migrated cell number in control group × 100 % .. Western blot Cells were collected at a density of 5×105 cells/well in 6-well plates after treatment with PCA (1, 2, and 5 μM) for 24 h. Each group of cells was harvested and washed twice with PBS and protein lysed in ice-cold radio immunoprecipitation assay buffer (RIPA; Beyotime, Shanghai, People’s Republic of China) with freshly added 0.01% protease inhibitor phenylmethanesulfonyl fluoride (Amresco, Shanghai, People’s Republic of China) and incubated on ice for 30 min. .. Cell lysis was centrifuged at 10,000× g for 5 min at 4°C, and the supernatant (20–30 μg of protein) was run on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis gel and transferred electrophoretically to a nitrocellulose membrane (Millipore, Shanghai, People’s Republic of China), then detected with JNK, phosphorylated (p-) JNK, P38, p-P38, RhoC, MTA1, and MMP-2/9 proteins.

    Incubation:

    Article Title: Portulacerebroside A inhibits adhesion, migration, and invasion of human leukemia HL60 cells and U937 cells through the regulation of p38/JNK signaling pathway
    Article Snippet: Migration rate = Migrated cell number in treated group Migrated cell number in control group × 100 % .. Western blot Cells were collected at a density of 5×105 cells/well in 6-well plates after treatment with PCA (1, 2, and 5 μM) for 24 h. Each group of cells was harvested and washed twice with PBS and protein lysed in ice-cold radio immunoprecipitation assay buffer (RIPA; Beyotime, Shanghai, People’s Republic of China) with freshly added 0.01% protease inhibitor phenylmethanesulfonyl fluoride (Amresco, Shanghai, People’s Republic of China) and incubated on ice for 30 min. .. Cell lysis was centrifuged at 10,000× g for 5 min at 4°C, and the supernatant (20–30 μg of protein) was run on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis gel and transferred electrophoretically to a nitrocellulose membrane (Millipore, Shanghai, People’s Republic of China), then detected with JNK, phosphorylated (p-) JNK, P38, p-P38, RhoC, MTA1, and MMP-2/9 proteins.

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  • 86
    Amresco phenylmethanesulfonylfluoride pmsf
    Phenylmethanesulfonylfluoride Pmsf, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethanesulfonylfluoride pmsf/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethanesulfonylfluoride pmsf - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Amresco phenylmethylsulfonyl fluoride pmsf
    Phenylmethylsulfonyl Fluoride Pmsf, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethylsulfonyl fluoride pmsf/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethylsulfonyl fluoride pmsf - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Amresco protease inhibitor pmsf
    Protease Inhibitor Pmsf, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor pmsf/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor pmsf - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Amresco phenylmethanesulfonyl fluoride
    Phenylmethanesulfonyl Fluoride, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenylmethanesulfonyl fluoride/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenylmethanesulfonyl fluoride - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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