protease inhibitor mixture  (Selleck Chemicals)

 
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  • 99
    Name:
    Protease Inhibitor Library
    Description:

    Catalog Number:
    l2500
    Price:
    None
    Category:
    Screening Libraries
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    Structured Review

    Selleck Chemicals protease inhibitor mixture

    https://www.bioz.com/result/protease inhibitor mixture/product/Selleck Chemicals
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor mixture - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa
    Article Snippet: .. Two days after transfection, cells were lysed with Pierce IP Lysis Buffer (Thermo Scientific) supplemented with 1% (vol/vol) protease inhibitor mixture (Selleck) for 40 min at 4 °C and then clarified by centrifugation at 12,000 × g for 20 min. .. The lysates were mixed accordingly (TCTE1-GFP or GFP with individual DRCs) and precleared with 20 µL Protein A/G magnetic beads (Selleck) for 1 h at 4 °C.

    Article Title: Cereblon suppresses the lipopolysaccharide-induced inflammatory response by promoting the ubiquitination and degradation of c-Jun
    Article Snippet: .. 48 h after transfection, cells were treated with CHX (200 μg/ml, Sigma) for the indicated time, washed with ice-cold PBS, and lysed in modified RIPA buffer (150 m m Tris-HCl (pH 7.4), 150 m m NaCl, 1% Triton X-100, 0.1% SDS, and 1 m m EDTA) containing freshly added protease inhibitor mixture (Selleck, ) with brief sonication to obtain cell lysates for immunoblot analyses. .. SF–c-Jun was purified with the Strep-Tactin–agarose beads.

    Fluorescence:

    Article Title: Validating Enterovirus D68-2Apro as an Antiviral Drug Target and the Discovery of Telaprevir as a Potent D68-2Apro Inhibitor
    Article Snippet: .. Screening of the Selleckchem protease inhibitor library using a fluorescence resonance energy transfer (FRET) assay allowed us to identify telaprevir (VX-950) ( ) as a potent inhibitor of EV-D68 by targeting the viral 2Apro . .. The close analog of telaprevir, boceprevir , had no enzymatic inhibition against 2Apro or antiviral activity, suggesting that the mechanism of action of telaprevir is specific toward 2Apro .

    Protease Inhibitor:

    Article Title: Cancer Cell-derived Exosomes Induce Mitogen-activated Protein Kinase-dependent Monocyte Survival by Transport of Functional Receptor Tyrosine Kinases *
    Article Snippet: .. Sorafenib, PD0325901, SCH772984, gefitinib, CP-724714, lapatinib, and protease inhibitor mixture were purchased from SelleckChem (Houston, TX). .. LPS, puromycin, saponin, proteinase K, and lidocaine hydrochloride were purchased from Sigma-Aldrich (Shanghai, China).

    Article Title: TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa
    Article Snippet: .. Two days after transfection, cells were lysed with Pierce IP Lysis Buffer (Thermo Scientific) supplemented with 1% (vol/vol) protease inhibitor mixture (Selleck) for 40 min at 4 °C and then clarified by centrifugation at 12,000 × g for 20 min. .. The lysates were mixed accordingly (TCTE1-GFP or GFP with individual DRCs) and precleared with 20 µL Protein A/G magnetic beads (Selleck) for 1 h at 4 °C.

    Article Title: The chromatin remodeler Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes
    Article Snippet: .. Cells were lysed in co-IP buffer (50 m m Tris-HCl (pH7.5), 150 m m NaCl, 10% glycerol, 2 m m EDTA, and 0.5% Nonidet P-40) with fresh addition of 1 m m PMSF (Sigma) for Western blotting and protease inhibitor mixture (Selleck) according to the instructions of the manufacturer for immunoprecipitation. .. The primary antibodies used included Chd4 (Bethyl), tubulin (Sigma), H2A.Z (Proteintech), H2A.X (Proteintech), Gapdh (Bioworld), and ubiquitin (Cell Signaling Technology).

    Article Title: Cereblon suppresses the lipopolysaccharide-induced inflammatory response by promoting the ubiquitination and degradation of c-Jun
    Article Snippet: .. 48 h after transfection, cells were treated with CHX (200 μg/ml, Sigma) for the indicated time, washed with ice-cold PBS, and lysed in modified RIPA buffer (150 m m Tris-HCl (pH 7.4), 150 m m NaCl, 1% Triton X-100, 0.1% SDS, and 1 m m EDTA) containing freshly added protease inhibitor mixture (Selleck, ) with brief sonication to obtain cell lysates for immunoblot analyses. .. SF–c-Jun was purified with the Strep-Tactin–agarose beads.

    Article Title: Validating Enterovirus D68-2Apro as an Antiviral Drug Target and the Discovery of Telaprevir as a Potent D68-2Apro Inhibitor
    Article Snippet: .. Screening of the Selleckchem protease inhibitor library using a fluorescence resonance energy transfer (FRET) assay allowed us to identify telaprevir (VX-950) ( ) as a potent inhibitor of EV-D68 by targeting the viral 2Apro . .. The close analog of telaprevir, boceprevir , had no enzymatic inhibition against 2Apro or antiviral activity, suggesting that the mechanism of action of telaprevir is specific toward 2Apro .

    Western Blot:

    Article Title: The chromatin remodeler Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes
    Article Snippet: .. Cells were lysed in co-IP buffer (50 m m Tris-HCl (pH7.5), 150 m m NaCl, 10% glycerol, 2 m m EDTA, and 0.5% Nonidet P-40) with fresh addition of 1 m m PMSF (Sigma) for Western blotting and protease inhibitor mixture (Selleck) according to the instructions of the manufacturer for immunoprecipitation. .. The primary antibodies used included Chd4 (Bethyl), tubulin (Sigma), H2A.Z (Proteintech), H2A.X (Proteintech), Gapdh (Bioworld), and ubiquitin (Cell Signaling Technology).

    Centrifugation:

    Article Title: TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa
    Article Snippet: .. Two days after transfection, cells were lysed with Pierce IP Lysis Buffer (Thermo Scientific) supplemented with 1% (vol/vol) protease inhibitor mixture (Selleck) for 40 min at 4 °C and then clarified by centrifugation at 12,000 × g for 20 min. .. The lysates were mixed accordingly (TCTE1-GFP or GFP with individual DRCs) and precleared with 20 µL Protein A/G magnetic beads (Selleck) for 1 h at 4 °C.

    Förster Resonance Energy Transfer:

    Article Title: Validating Enterovirus D68-2Apro as an Antiviral Drug Target and the Discovery of Telaprevir as a Potent D68-2Apro Inhibitor
    Article Snippet: .. Screening of the Selleckchem protease inhibitor library using a fluorescence resonance energy transfer (FRET) assay allowed us to identify telaprevir (VX-950) ( ) as a potent inhibitor of EV-D68 by targeting the viral 2Apro . .. The close analog of telaprevir, boceprevir , had no enzymatic inhibition against 2Apro or antiviral activity, suggesting that the mechanism of action of telaprevir is specific toward 2Apro .

    Immunoprecipitation:

    Article Title: The chromatin remodeler Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes
    Article Snippet: .. Cells were lysed in co-IP buffer (50 m m Tris-HCl (pH7.5), 150 m m NaCl, 10% glycerol, 2 m m EDTA, and 0.5% Nonidet P-40) with fresh addition of 1 m m PMSF (Sigma) for Western blotting and protease inhibitor mixture (Selleck) according to the instructions of the manufacturer for immunoprecipitation. .. The primary antibodies used included Chd4 (Bethyl), tubulin (Sigma), H2A.Z (Proteintech), H2A.X (Proteintech), Gapdh (Bioworld), and ubiquitin (Cell Signaling Technology).

    Co-Immunoprecipitation Assay:

    Article Title: The chromatin remodeler Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes
    Article Snippet: .. Cells were lysed in co-IP buffer (50 m m Tris-HCl (pH7.5), 150 m m NaCl, 10% glycerol, 2 m m EDTA, and 0.5% Nonidet P-40) with fresh addition of 1 m m PMSF (Sigma) for Western blotting and protease inhibitor mixture (Selleck) according to the instructions of the manufacturer for immunoprecipitation. .. The primary antibodies used included Chd4 (Bethyl), tubulin (Sigma), H2A.Z (Proteintech), H2A.X (Proteintech), Gapdh (Bioworld), and ubiquitin (Cell Signaling Technology).

    Modification:

    Article Title: Cereblon suppresses the lipopolysaccharide-induced inflammatory response by promoting the ubiquitination and degradation of c-Jun
    Article Snippet: .. 48 h after transfection, cells were treated with CHX (200 μg/ml, Sigma) for the indicated time, washed with ice-cold PBS, and lysed in modified RIPA buffer (150 m m Tris-HCl (pH 7.4), 150 m m NaCl, 1% Triton X-100, 0.1% SDS, and 1 m m EDTA) containing freshly added protease inhibitor mixture (Selleck, ) with brief sonication to obtain cell lysates for immunoblot analyses. .. SF–c-Jun was purified with the Strep-Tactin–agarose beads.

    Sonication:

    Article Title: Cereblon suppresses the lipopolysaccharide-induced inflammatory response by promoting the ubiquitination and degradation of c-Jun
    Article Snippet: .. 48 h after transfection, cells were treated with CHX (200 μg/ml, Sigma) for the indicated time, washed with ice-cold PBS, and lysed in modified RIPA buffer (150 m m Tris-HCl (pH 7.4), 150 m m NaCl, 1% Triton X-100, 0.1% SDS, and 1 m m EDTA) containing freshly added protease inhibitor mixture (Selleck, ) with brief sonication to obtain cell lysates for immunoblot analyses. .. SF–c-Jun was purified with the Strep-Tactin–agarose beads.

    Lysis:

    Article Title: TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa
    Article Snippet: .. Two days after transfection, cells were lysed with Pierce IP Lysis Buffer (Thermo Scientific) supplemented with 1% (vol/vol) protease inhibitor mixture (Selleck) for 40 min at 4 °C and then clarified by centrifugation at 12,000 × g for 20 min. .. The lysates were mixed accordingly (TCTE1-GFP or GFP with individual DRCs) and precleared with 20 µL Protein A/G magnetic beads (Selleck) for 1 h at 4 °C.

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  • 99
    Selleck Chemicals protease inhibitor mixture
    Protease Inhibitor Mixture, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor mixture/product/Selleck Chemicals
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor mixture - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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