protease inhibitor mixture kit  (Thermo Fisher)


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  • 99
    Name:
    Protease Inhibitor Cocktails
    Description:

    Catalog Number:
    78410
    Price:
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    Structured Review

    Thermo Fisher protease inhibitor mixture kit

    https://www.bioz.com/result/protease inhibitor mixture kit/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor mixture kit - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Transduction:

    Article Title: FIH-1/c-Kit Signaling: A Novel Contributor to Corneal Epithelial Glycogen Metabolism
    Article Snippet: .. HCEKs transduced with retroviral supernatants carrying either LZRS-empty vector or FIH-124 with or without SCF were lysed with radioimmunoprecipitation assay (RIPA) buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.25% deoxycholic acid, 1% Tergitol Nonidet P-40 (NP-40), 1 mM EDTA, 2 mM dithiothreitol, 1 mM phenylmethylsulphonyl fluoride, phosphatase inhibitor cocktail (Thermo Scientific, West Palm Beach, FL), and protease inhibitor cocktail (Thermo Scientific), followed by centrifugation at 15,000 g for 15 minutes. ..

    Transfection:

    Article Title: MURC deficiency in smooth muscle attenuates pulmonary hypertension
    Article Snippet: .. Immunoprecipitation COS cells transfected with each of the plasmids using FuGENE6 (Roche Applied Science) were washed with ice-cold PBS and lysed with lysis buffer (50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1% Nonidet P-40) containing protease inhibitory cocktail (Pierce), 1 mM Na3 VO4 , 1 mM NaF, 1 mM phenylmethylsulfonyl fluoride, and 60 mM octyl glucoside. .. Immunoprecipitation was carried out by incubating the same amount of cell lysates with magnetic beads (Magnosphere MS300/Carboxyl, COSMO BIO, Tokyo, Japan) coated with each antibody at 4 °C overnight.

    Radio Immunoprecipitation:

    Article Title: FIH-1/c-Kit Signaling: A Novel Contributor to Corneal Epithelial Glycogen Metabolism
    Article Snippet: .. HCEKs transduced with retroviral supernatants carrying either LZRS-empty vector or FIH-124 with or without SCF were lysed with radioimmunoprecipitation assay (RIPA) buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.25% deoxycholic acid, 1% Tergitol Nonidet P-40 (NP-40), 1 mM EDTA, 2 mM dithiothreitol, 1 mM phenylmethylsulphonyl fluoride, phosphatase inhibitor cocktail (Thermo Scientific, West Palm Beach, FL), and protease inhibitor cocktail (Thermo Scientific), followed by centrifugation at 15,000 g for 15 minutes. ..

    Protease Inhibitor:

    Article Title: The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function
    Article Snippet: .. Cells were lysed in lysis buffer (10 mM HEPES pH 7, 100 mM KCl, 2 mM EDTA, 0.5% NP-40, 2 mM DTT, 50 U/ml RNaseOUT (Invitrogen) and Complete protease inhibitor cocktail) for 15 min on ice and the lysate was cleared by centrifugation at 13 000g for 15 min at 4°C. .. Protein concentration was determined using Bradford reagent.

    Article Title: Inhibition of vascular smooth muscle growth via signaling crosstalk between AMP-activated protein kinase and cAMP-dependent protein kinase
    Article Snippet: .. For specific activity, treated cells were lysed in buffer [50 mM Tris, pH 6.8; 1% SDS; 0.1% Triton; protease inhibitor cocktail (Thermo); phosphate inhibitor cocktail (Thermo)], and added to wells of a 96-well microtiter plate. .. ELISA-based activity assays were performed per manufacturer's instructions.

    Article Title: Fragment Screening of Human Aquaporin 1
    Article Snippet: .. The cell pellet was resuspended in 5 mL/g lysis buffer (20 mM Tris-HCl pH 8, 300 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 10% glycerol (v /v ), benzonase (25 U/mL) (Novagen, Madison, WI, USA), complete protease inhibitor cocktail and 2% n -Octyl-β-d -Glucopyranoside (OG, Anagrade, Affymetrix, Singapore)). .. Cell lysis was achieved with 10 min of sonication followed by passing through a microfluidizer (Microfluidics, Westwood, MA, USA) 5 times.

    Article Title: FIH-1/c-Kit Signaling: A Novel Contributor to Corneal Epithelial Glycogen Metabolism
    Article Snippet: .. HCEKs transduced with retroviral supernatants carrying either LZRS-empty vector or FIH-124 with or without SCF were lysed with radioimmunoprecipitation assay (RIPA) buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.25% deoxycholic acid, 1% Tergitol Nonidet P-40 (NP-40), 1 mM EDTA, 2 mM dithiothreitol, 1 mM phenylmethylsulphonyl fluoride, phosphatase inhibitor cocktail (Thermo Scientific, West Palm Beach, FL), and protease inhibitor cocktail (Thermo Scientific), followed by centrifugation at 15,000 g for 15 minutes. ..

    Article Title:
    Article Snippet: .. Plates were placed on ice, PBS was aspirated, and cells were lysed by adding 50 μ l of lysis buffer (Pierce, Thermo Scientific Rockford, IL), supplemented with 1% phosphatase inhibitor cocktail 3, 100 nM okadaic acid, and 1% protease inhibitor (Pierce, Thermo Scientific) per well. .. Cells were scraped and centrifuged at 2000 rpm at 4°C for 3 minutes, and the supernatant was collected and prepared for SDS-PAGE following the NuPAGE protocol (Novex, Life Technologies Grand Island, NY).

    Article Title: FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules
    Article Snippet: .. Western blotting Tissue samples were homogenized in RIPA lysis buffer containing 1 mM DTT, 0.2 mM PMSF and 1X protease inhibitor cocktail, and the lysates were cleared by centrifugation at 14000 × g for 5 min. For ontogenesis studies protein concentration was measured using Pierce BCA protein assay kit (Life Technologies, 23227); absorbance was measured with a Victor2 plate reader (Wallac, Turku, Finland). .. Samples diluted in Laemmli buffer were incubated 5 min at 95°C before loading them on the gel.

    BIA-KA:

    Article Title: FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules
    Article Snippet: .. Western blotting Tissue samples were homogenized in RIPA lysis buffer containing 1 mM DTT, 0.2 mM PMSF and 1X protease inhibitor cocktail, and the lysates were cleared by centrifugation at 14000 × g for 5 min. For ontogenesis studies protein concentration was measured using Pierce BCA protein assay kit (Life Technologies, 23227); absorbance was measured with a Victor2 plate reader (Wallac, Turku, Finland). .. Samples diluted in Laemmli buffer were incubated 5 min at 95°C before loading them on the gel.

    Centrifugation:

    Article Title: The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function
    Article Snippet: .. Cells were lysed in lysis buffer (10 mM HEPES pH 7, 100 mM KCl, 2 mM EDTA, 0.5% NP-40, 2 mM DTT, 50 U/ml RNaseOUT (Invitrogen) and Complete protease inhibitor cocktail) for 15 min on ice and the lysate was cleared by centrifugation at 13 000g for 15 min at 4°C. .. Protein concentration was determined using Bradford reagent.

    Article Title: FIH-1/c-Kit Signaling: A Novel Contributor to Corneal Epithelial Glycogen Metabolism
    Article Snippet: .. HCEKs transduced with retroviral supernatants carrying either LZRS-empty vector or FIH-124 with or without SCF were lysed with radioimmunoprecipitation assay (RIPA) buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.25% deoxycholic acid, 1% Tergitol Nonidet P-40 (NP-40), 1 mM EDTA, 2 mM dithiothreitol, 1 mM phenylmethylsulphonyl fluoride, phosphatase inhibitor cocktail (Thermo Scientific, West Palm Beach, FL), and protease inhibitor cocktail (Thermo Scientific), followed by centrifugation at 15,000 g for 15 minutes. ..

    Article Title: FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules
    Article Snippet: .. Western blotting Tissue samples were homogenized in RIPA lysis buffer containing 1 mM DTT, 0.2 mM PMSF and 1X protease inhibitor cocktail, and the lysates were cleared by centrifugation at 14000 × g for 5 min. For ontogenesis studies protein concentration was measured using Pierce BCA protein assay kit (Life Technologies, 23227); absorbance was measured with a Victor2 plate reader (Wallac, Turku, Finland). .. Samples diluted in Laemmli buffer were incubated 5 min at 95°C before loading them on the gel.

    Immunoprecipitation:

    Article Title: MURC deficiency in smooth muscle attenuates pulmonary hypertension
    Article Snippet: .. Immunoprecipitation COS cells transfected with each of the plasmids using FuGENE6 (Roche Applied Science) were washed with ice-cold PBS and lysed with lysis buffer (50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1% Nonidet P-40) containing protease inhibitory cocktail (Pierce), 1 mM Na3 VO4 , 1 mM NaF, 1 mM phenylmethylsulfonyl fluoride, and 60 mM octyl glucoside. .. Immunoprecipitation was carried out by incubating the same amount of cell lysates with magnetic beads (Magnosphere MS300/Carboxyl, COSMO BIO, Tokyo, Japan) coated with each antibody at 4 °C overnight.

    Activity Assay:

    Article Title: Inhibition of vascular smooth muscle growth via signaling crosstalk between AMP-activated protein kinase and cAMP-dependent protein kinase
    Article Snippet: .. For specific activity, treated cells were lysed in buffer [50 mM Tris, pH 6.8; 1% SDS; 0.1% Triton; protease inhibitor cocktail (Thermo); phosphate inhibitor cocktail (Thermo)], and added to wells of a 96-well microtiter plate. .. ELISA-based activity assays were performed per manufacturer's instructions.

    Protein Concentration:

    Article Title: FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules
    Article Snippet: .. Western blotting Tissue samples were homogenized in RIPA lysis buffer containing 1 mM DTT, 0.2 mM PMSF and 1X protease inhibitor cocktail, and the lysates were cleared by centrifugation at 14000 × g for 5 min. For ontogenesis studies protein concentration was measured using Pierce BCA protein assay kit (Life Technologies, 23227); absorbance was measured with a Victor2 plate reader (Wallac, Turku, Finland). .. Samples diluted in Laemmli buffer were incubated 5 min at 95°C before loading them on the gel.

    Western Blot:

    Article Title: FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules
    Article Snippet: .. Western blotting Tissue samples were homogenized in RIPA lysis buffer containing 1 mM DTT, 0.2 mM PMSF and 1X protease inhibitor cocktail, and the lysates were cleared by centrifugation at 14000 × g for 5 min. For ontogenesis studies protein concentration was measured using Pierce BCA protein assay kit (Life Technologies, 23227); absorbance was measured with a Victor2 plate reader (Wallac, Turku, Finland). .. Samples diluted in Laemmli buffer were incubated 5 min at 95°C before loading them on the gel.

    Lysis:

    Article Title: MURC deficiency in smooth muscle attenuates pulmonary hypertension
    Article Snippet: .. Immunoprecipitation COS cells transfected with each of the plasmids using FuGENE6 (Roche Applied Science) were washed with ice-cold PBS and lysed with lysis buffer (50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1% Nonidet P-40) containing protease inhibitory cocktail (Pierce), 1 mM Na3 VO4 , 1 mM NaF, 1 mM phenylmethylsulfonyl fluoride, and 60 mM octyl glucoside. .. Immunoprecipitation was carried out by incubating the same amount of cell lysates with magnetic beads (Magnosphere MS300/Carboxyl, COSMO BIO, Tokyo, Japan) coated with each antibody at 4 °C overnight.

    Article Title: The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function
    Article Snippet: .. Cells were lysed in lysis buffer (10 mM HEPES pH 7, 100 mM KCl, 2 mM EDTA, 0.5% NP-40, 2 mM DTT, 50 U/ml RNaseOUT (Invitrogen) and Complete protease inhibitor cocktail) for 15 min on ice and the lysate was cleared by centrifugation at 13 000g for 15 min at 4°C. .. Protein concentration was determined using Bradford reagent.

    Article Title: Fragment Screening of Human Aquaporin 1
    Article Snippet: .. The cell pellet was resuspended in 5 mL/g lysis buffer (20 mM Tris-HCl pH 8, 300 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 10% glycerol (v /v ), benzonase (25 U/mL) (Novagen, Madison, WI, USA), complete protease inhibitor cocktail and 2% n -Octyl-β-d -Glucopyranoside (OG, Anagrade, Affymetrix, Singapore)). .. Cell lysis was achieved with 10 min of sonication followed by passing through a microfluidizer (Microfluidics, Westwood, MA, USA) 5 times.

    Article Title:
    Article Snippet: .. Plates were placed on ice, PBS was aspirated, and cells were lysed by adding 50 μ l of lysis buffer (Pierce, Thermo Scientific Rockford, IL), supplemented with 1% phosphatase inhibitor cocktail 3, 100 nM okadaic acid, and 1% protease inhibitor (Pierce, Thermo Scientific) per well. .. Cells were scraped and centrifuged at 2000 rpm at 4°C for 3 minutes, and the supernatant was collected and prepared for SDS-PAGE following the NuPAGE protocol (Novex, Life Technologies Grand Island, NY).

    Article Title: FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules
    Article Snippet: .. Western blotting Tissue samples were homogenized in RIPA lysis buffer containing 1 mM DTT, 0.2 mM PMSF and 1X protease inhibitor cocktail, and the lysates were cleared by centrifugation at 14000 × g for 5 min. For ontogenesis studies protein concentration was measured using Pierce BCA protein assay kit (Life Technologies, 23227); absorbance was measured with a Victor2 plate reader (Wallac, Turku, Finland). .. Samples diluted in Laemmli buffer were incubated 5 min at 95°C before loading them on the gel.

    Plasmid Preparation:

    Article Title: FIH-1/c-Kit Signaling: A Novel Contributor to Corneal Epithelial Glycogen Metabolism
    Article Snippet: .. HCEKs transduced with retroviral supernatants carrying either LZRS-empty vector or FIH-124 with or without SCF were lysed with radioimmunoprecipitation assay (RIPA) buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.25% deoxycholic acid, 1% Tergitol Nonidet P-40 (NP-40), 1 mM EDTA, 2 mM dithiothreitol, 1 mM phenylmethylsulphonyl fluoride, phosphatase inhibitor cocktail (Thermo Scientific, West Palm Beach, FL), and protease inhibitor cocktail (Thermo Scientific), followed by centrifugation at 15,000 g for 15 minutes. ..

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