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Nacalai protease inhibitor mix
Protease Inhibitor Mix, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protease inhibitor mix/product/Nacalai
Average 92 stars, based on 12 article reviews
Price from $9.99 to $1999.99
protease inhibitor mix - by Bioz Stars, 2020-11
92/100 stars

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Related Articles

Nucleic Acid Electrophoresis:

Article Title: Intramembrane Processing by Signal Peptide Peptidase Regulates the Membrane Localization of Hepatitis C Virus Core Protein and Viral Propagation ▿
Article Snippet: .. Cells were lysed on ice in Triton lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton-X 100, 10% glycerol) supplemented with a protease inhibitor mix (Nacalai Tesque, Kyoto, Japan) at 24 or 48 h after transfection and were then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Tris-glycine buffer and Western blotting using appropriate antibodies as previously described ( ). .. The stained protein bands were visualized using the SuperSignal West Femto enhanced-chemiluminescence substrate (Pierce, Rockford, IL) and an LAS3000 imaging system (Fuji Photo Film, Tokyo, Japan).

Transfection:

Article Title: Intramembrane Processing by Signal Peptide Peptidase Regulates the Membrane Localization of Hepatitis C Virus Core Protein and Viral Propagation ▿
Article Snippet: .. Cells were lysed on ice in Triton lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton-X 100, 10% glycerol) supplemented with a protease inhibitor mix (Nacalai Tesque, Kyoto, Japan) at 24 or 48 h after transfection and were then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Tris-glycine buffer and Western blotting using appropriate antibodies as previously described ( ). .. The stained protein bands were visualized using the SuperSignal West Femto enhanced-chemiluminescence substrate (Pierce, Rockford, IL) and an LAS3000 imaging system (Fuji Photo Film, Tokyo, Japan).

Radio Immunoprecipitation:

Article Title: Small extracellular vesicles from rat plasma promote migration and proliferation of vascular smooth muscle cells
Article Snippet: .. Protein lysates of sEV were extracted using a radio-immunoprecipitation assay buffer containing 20 mM Tris-HCl (pH7.5), 150 mM NaCl, 10 mM MgCl2 , 1% Triton-X, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 1% protease inhibitor mixture, and 1% phosphatase inhibitor mixture (Nacalai Tesque) (4°C, 10 min). .. Protein concentration was measured by using a Pierce BCA protein assay kit (Pierce, Rockford, IL, USA).

Protease Inhibitor:

Article Title: Intramembrane Processing by Signal Peptide Peptidase Regulates the Membrane Localization of Hepatitis C Virus Core Protein and Viral Propagation ▿
Article Snippet: .. Cells were lysed on ice in Triton lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton-X 100, 10% glycerol) supplemented with a protease inhibitor mix (Nacalai Tesque, Kyoto, Japan) at 24 or 48 h after transfection and were then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Tris-glycine buffer and Western blotting using appropriate antibodies as previously described ( ). .. The stained protein bands were visualized using the SuperSignal West Femto enhanced-chemiluminescence substrate (Pierce, Rockford, IL) and an LAS3000 imaging system (Fuji Photo Film, Tokyo, Japan).

Article Title: Quasispecies of Hepatitis C Virus Participate in Cell-Specific Infectivity
Article Snippet: .. Immunoblotting Cells lysed on ice in lysis buffer (20 mM Tris-HCl [pH7.4], 135 mM NaCl, 1% Triton-X 100, 10% glycerol) supplemented with a protease inhibitor mix (Nacalai Tesque) were boiled in loading buffer and subjected to 5–20% gradient SDS-PAGE. .. The proteins were transferred to polyvinylidene difluoride membranes (Millipore) and reacted with the appropriate antibodies.

Article Title: N-Glycosylation of the I-like Domain of ?1 Integrin Is Essential for ?1 Integrin Expression and Biological Function
Article Snippet: .. Immunoprecipitation and Western Blot —Cells were washed with ice-cold phosphate-buffered saline (PBS) and lysed in lysis buffer containing 20 m m Tris-HCl (pH 7.4), 150 m m NaCl, 1% (w/v) Triton X-100, and Protease Inhibitor Mixture (Nacalai Tesque, Kyoto, Japan). .. The supernatants were collected, and protein concentrations were determined using the BCA protein assay kit (Pierce).

Article Title: Intramembrane Processing by Signal Peptide Peptidase Regulates the Membrane Localization of Hepatitis C Virus Core Protein and Viral Propagation ▿
Article Snippet: .. Collected cells were suspended in 0.6 ml of TNE buffer (25 mM Tris-HCl [pH 7.4] containing 150 mM NaCl, a protease inhibitor mix [Nacalai Tesque], and 5 mM EDTA) and then homogenated with a Dounce homogenizer or suspended with a 24-gauge needle. .. Each homogenate was incubated for 30 min on ice with or without 1% Triton X-100.

Article Title: Small extracellular vesicles from rat plasma promote migration and proliferation of vascular smooth muscle cells
Article Snippet: .. Protein lysates of sEV were extracted using a radio-immunoprecipitation assay buffer containing 20 mM Tris-HCl (pH7.5), 150 mM NaCl, 10 mM MgCl2 , 1% Triton-X, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 1% protease inhibitor mixture, and 1% phosphatase inhibitor mixture (Nacalai Tesque) (4°C, 10 min). .. Protein concentration was measured by using a Pierce BCA protein assay kit (Pierce, Rockford, IL, USA).

Article Title: Diverse distribution of tyrosine receptor kinase B isoforms in rat multiple tissues
Article Snippet: .. Protein lysates were obtained from mashed rat tissues using Cell Lysis Buffer (Cell Signaling Technology) containing 20 mM Tris-HCl (pH7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton-X, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4 and 1 µ g/ml leupeptin supplemented with 0.1% protease inhibitor mixture (Nacalai Tesque, Kyoto, Japan) on ice. .. Protein concentration was determined by using a bicinchoninic acid method (Pierce, Rockford, IL, U.S.A.).

Article Title: Acquisition of Complement Resistance through Incorporation of CD55/Decay-Accelerating Factor into Viral Particles Bearing Baculovirus GP64 ▿
Article Snippet: .. Collected cells were suspended in 1 ml of TNE buffer (25 mM Tris-HCl [pH 7.4] containing 150 mM NaCl, a protease inhibitor mix [Nacalai Tesque, Tokyo, Japan], and 5 mM EDTA) and then homogenized with a Dounce homogenizer or suspended with a 24-gauge needle. .. Each homogenate was incubated for 30 min on ice with 0.2% Triton X-100 (TX-100).

Article Title: Predominant localization of phosphatidylserine at the cytoplasmic leaflet of the ER, and its TMEM16K-dependent redistribution
Article Snippet: .. The yeast cell walls were removed by treating the replicas for 2 h at 30 °C with 0.5% Westase (Takara Bio) in McIlvain citrate-phosphate buffer (pH 6.0) containing 10 mM EDTA, 30% FCS, and a protease inhibitor mixture (Nacalai), followed by treatment at 60 °C overnight with 2.5% SDS. ..

Immunoprecipitation:

Article Title: N-Glycosylation of the I-like Domain of ?1 Integrin Is Essential for ?1 Integrin Expression and Biological Function
Article Snippet: .. Immunoprecipitation and Western Blot —Cells were washed with ice-cold phosphate-buffered saline (PBS) and lysed in lysis buffer containing 20 m m Tris-HCl (pH 7.4), 150 m m NaCl, 1% (w/v) Triton X-100, and Protease Inhibitor Mixture (Nacalai Tesque, Kyoto, Japan). .. The supernatants were collected, and protein concentrations were determined using the BCA protein assay kit (Pierce).

Lysis:

Article Title: Intramembrane Processing by Signal Peptide Peptidase Regulates the Membrane Localization of Hepatitis C Virus Core Protein and Viral Propagation ▿
Article Snippet: .. Cells were lysed on ice in Triton lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton-X 100, 10% glycerol) supplemented with a protease inhibitor mix (Nacalai Tesque, Kyoto, Japan) at 24 or 48 h after transfection and were then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Tris-glycine buffer and Western blotting using appropriate antibodies as previously described ( ). .. The stained protein bands were visualized using the SuperSignal West Femto enhanced-chemiluminescence substrate (Pierce, Rockford, IL) and an LAS3000 imaging system (Fuji Photo Film, Tokyo, Japan).

Article Title: Quasispecies of Hepatitis C Virus Participate in Cell-Specific Infectivity
Article Snippet: .. Immunoblotting Cells lysed on ice in lysis buffer (20 mM Tris-HCl [pH7.4], 135 mM NaCl, 1% Triton-X 100, 10% glycerol) supplemented with a protease inhibitor mix (Nacalai Tesque) were boiled in loading buffer and subjected to 5–20% gradient SDS-PAGE. .. The proteins were transferred to polyvinylidene difluoride membranes (Millipore) and reacted with the appropriate antibodies.

Article Title: N-Glycosylation of the I-like Domain of ?1 Integrin Is Essential for ?1 Integrin Expression and Biological Function
Article Snippet: .. Immunoprecipitation and Western Blot —Cells were washed with ice-cold phosphate-buffered saline (PBS) and lysed in lysis buffer containing 20 m m Tris-HCl (pH 7.4), 150 m m NaCl, 1% (w/v) Triton X-100, and Protease Inhibitor Mixture (Nacalai Tesque, Kyoto, Japan). .. The supernatants were collected, and protein concentrations were determined using the BCA protein assay kit (Pierce).

Article Title: Diverse distribution of tyrosine receptor kinase B isoforms in rat multiple tissues
Article Snippet: .. Protein lysates were obtained from mashed rat tissues using Cell Lysis Buffer (Cell Signaling Technology) containing 20 mM Tris-HCl (pH7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton-X, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4 and 1 µ g/ml leupeptin supplemented with 0.1% protease inhibitor mixture (Nacalai Tesque, Kyoto, Japan) on ice. .. Protein concentration was determined by using a bicinchoninic acid method (Pierce, Rockford, IL, U.S.A.).

Western Blot:

Article Title: Intramembrane Processing by Signal Peptide Peptidase Regulates the Membrane Localization of Hepatitis C Virus Core Protein and Viral Propagation ▿
Article Snippet: .. Cells were lysed on ice in Triton lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton-X 100, 10% glycerol) supplemented with a protease inhibitor mix (Nacalai Tesque, Kyoto, Japan) at 24 or 48 h after transfection and were then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Tris-glycine buffer and Western blotting using appropriate antibodies as previously described ( ). .. The stained protein bands were visualized using the SuperSignal West Femto enhanced-chemiluminescence substrate (Pierce, Rockford, IL) and an LAS3000 imaging system (Fuji Photo Film, Tokyo, Japan).

Article Title: N-Glycosylation of the I-like Domain of ?1 Integrin Is Essential for ?1 Integrin Expression and Biological Function
Article Snippet: .. Immunoprecipitation and Western Blot —Cells were washed with ice-cold phosphate-buffered saline (PBS) and lysed in lysis buffer containing 20 m m Tris-HCl (pH 7.4), 150 m m NaCl, 1% (w/v) Triton X-100, and Protease Inhibitor Mixture (Nacalai Tesque, Kyoto, Japan). .. The supernatants were collected, and protein concentrations were determined using the BCA protein assay kit (Pierce).

SDS Page:

Article Title: Intramembrane Processing by Signal Peptide Peptidase Regulates the Membrane Localization of Hepatitis C Virus Core Protein and Viral Propagation ▿
Article Snippet: .. Cells were lysed on ice in Triton lysis buffer (20 mM Tris-HCl [pH 7.4], 135 mM NaCl, 1% Triton-X 100, 10% glycerol) supplemented with a protease inhibitor mix (Nacalai Tesque, Kyoto, Japan) at 24 or 48 h after transfection and were then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Tris-glycine buffer and Western blotting using appropriate antibodies as previously described ( ). .. The stained protein bands were visualized using the SuperSignal West Femto enhanced-chemiluminescence substrate (Pierce, Rockford, IL) and an LAS3000 imaging system (Fuji Photo Film, Tokyo, Japan).

Article Title: Quasispecies of Hepatitis C Virus Participate in Cell-Specific Infectivity
Article Snippet: .. Immunoblotting Cells lysed on ice in lysis buffer (20 mM Tris-HCl [pH7.4], 135 mM NaCl, 1% Triton-X 100, 10% glycerol) supplemented with a protease inhibitor mix (Nacalai Tesque) were boiled in loading buffer and subjected to 5–20% gradient SDS-PAGE. .. The proteins were transferred to polyvinylidene difluoride membranes (Millipore) and reacted with the appropriate antibodies.

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  • 93
    Nacalai protease inhibitor mixture
    Protease Inhibitor Mixture, supplied by Nacalai, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor mixture/product/Nacalai
    Average 93 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor mixture - by Bioz Stars, 2020-11
    93/100 stars
      Buy from Supplier

    91
    Nacalai phosphatase inhibitor mixture
    Phosphatase Inhibitor Mixture, supplied by Nacalai, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase inhibitor mixture/product/Nacalai
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitor mixture - by Bioz Stars, 2020-11
    91/100 stars
      Buy from Supplier

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