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Boehringer Mannheim protease inhibitor mix
Protease Inhibitor Mix, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protease inhibitor mix/product/Boehringer Mannheim
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
protease inhibitor mix - by Bioz Stars, 2020-11
92/100 stars

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Radio Immunoprecipitation:

Article Title: CCAAT/Enhancer-Binding Protein Phosphorylation Biases Cortical Precursors to Generate Neurons Rather Than Astrocytes In Vivo
Article Snippet: .. For biochemical analysis, cells were washed with ice-cold HBSS and lysed directly in the dish with radioimmunoprecipitation assay (RIPA) buffer (50 m m Tris, pH 7.2, 150 m m NaCl, 2 m m EDTA, 1% NP-40, 1% Na deoxycholate, and 0.1% v/v SDS) supplemented with protease inhibitor mixture (Boehringer Mannheim) and 1.5 m m sodium vanadate. .. Lysates were scraped into Eppendorf tubes, rocked for 10 min at 4°C, and cleared by centrifugation.

Protease Inhibitor:

Article Title: Ca2+ permeability and Na+ conductance in cellular toxicity caused by hyperactive DEG/ENaC channels
Article Snippet: .. Three days after injection, 30 oocytes were resuspended in 0.3 M sucrose plus 10 mM sodium phosphate (pH 7.4) containing a protease inhibitor mixture (Complete; Boehringer-Mannheim), and homogenized with 20 strokes in a glass homogeneizer. .. Pelleted membranes were then resuspended in solubilization buffer containing (in mM): 150 NaCl, 50 Tris·HCl, 1 EDTA, and 1% Triton X-100; pH 7.5 plus protease inhibitor mixture and subjected to SDS-PAGE ( ).

Article Title: Anti-Inflammatory Drug Therapy Alters β-Amyloid Processing and Deposition in an Animal Model of Alzheimer's Disease
Article Snippet: .. Half of the brain was homogenized in 1 ml of PBS with 3× protease inhibitor mixture (Boehringer Mannheim, Mannheim, Germany). ..

Article Title: Extracellular matrix composition determines the transcriptional response to epidermal growth factor receptor activation
Article Snippet: .. Detergent extracts of whole cells were prepared by solubilization in 55 mM Tris/HCl, pH 7.4/132 mM NaCl/22 mM NaF/1 mM Na3 VO4 /11 mM sodium pyrophosphate/0.1% Triton X-100 containing complete protease inhibitor mixture (Boehringer Mannheim). .. Cell lysates were centrifuged at 13,000 × g for 5 min and supernatants assayed for protein content by using the Bradford dye-binding method (Bio-Rad).

Article Title: Structural and Functional Analysis of a Novel Coiled-Coil Protein Involved in Ypt6 GTPase-regulated Protein Transport in Yeast
Article Snippet: .. Thirty OD600 units of cells were collected and suspended in ice-cold lysis buffer (0.2 M Tris-Cl, pH 8.0, 6 mM MgCl2 , 1 mM EGTA, 1 mM DTT) containing 4 mM Pefabloc (Boehringer Mannheim) and 1/25 vol of protease inhibitor mixture (Complete TM, Boehringer Mannheim). ..

Article Title: CCAAT/Enhancer-Binding Protein Phosphorylation Biases Cortical Precursors to Generate Neurons Rather Than Astrocytes In Vivo
Article Snippet: .. For biochemical analysis, cells were washed with ice-cold HBSS and lysed directly in the dish with radioimmunoprecipitation assay (RIPA) buffer (50 m m Tris, pH 7.2, 150 m m NaCl, 2 m m EDTA, 1% NP-40, 1% Na deoxycholate, and 0.1% v/v SDS) supplemented with protease inhibitor mixture (Boehringer Mannheim) and 1.5 m m sodium vanadate. .. Lysates were scraped into Eppendorf tubes, rocked for 10 min at 4°C, and cleared by centrifugation.

Article Title: The plug domain of FepA, a TonB-dependent transport protein from Escherichia coli, binds its siderophore in the absence of the transmembrane barrel domain
Article Snippet: .. Cell pellets were resuspended in a 50% (wt/vol) mixture with a lysis buffer containing 1 mg/ml of chicken egg white lysozyme (Sigma), 50 mM Tris (pH 8.0), protease inhibitor mixture (one tablet per 100 ml of buffer, Boehringer Mannheim), 50 mM NaCl, and 6% glycerol, then sonicated while chilled on wet ice to lyse them. .. After lysis, 40 mg of protamine sulfate (Sigma) dissolved in water was added to precipitate nucleic acids.

Article Title: The Influence of EGFR Inactivation on the Radiation Response in High Grade Glioma
Article Snippet: .. Western Blotting Cells were lysed in lysis buffer (150 mM NaCl, 20 mM Tris–HCl (pH 7.5), 1% NP40, 1 mM EDTA, 1 mM EGTA, 1 mM sodium orthovanadate, and protease inhibitor mixture (Boehringer Mannheim Co., Barcelona, Spain) at 4 °C. ..

Article Title: Role of the cytoplasmic tyrosines of ?1A integrins in transformation by v-src
Article Snippet: .. Briefly, cells were incubated on ice in lysis buffer containing 100 mM sodium chloride, 2 mM EDTA, 1 mM sodium orthovanadate, 0.5 mM sodium molybdate, protease inhibitor mixture (Boehringer Mannheim), 10 mM Tris⋅HCl (pH 7.6), and 0.5% (wt/vol) 3-[(3-cholamidopropyl)dimethylammonio]-1-peopanesulfonate. .. After centrifugation, the supernatants were precleared with agarose beads coupled with antibodies to rat IgG and IgM (American Qualex, La Mirada, CA) and subsequently incubated with rat MB1.2 antibody against mouse β1.

Incubation:

Article Title: Role of the cytoplasmic tyrosines of ?1A integrins in transformation by v-src
Article Snippet: .. Briefly, cells were incubated on ice in lysis buffer containing 100 mM sodium chloride, 2 mM EDTA, 1 mM sodium orthovanadate, 0.5 mM sodium molybdate, protease inhibitor mixture (Boehringer Mannheim), 10 mM Tris⋅HCl (pH 7.6), and 0.5% (wt/vol) 3-[(3-cholamidopropyl)dimethylammonio]-1-peopanesulfonate. .. After centrifugation, the supernatants were precleared with agarose beads coupled with antibodies to rat IgG and IgM (American Qualex, La Mirada, CA) and subsequently incubated with rat MB1.2 antibody against mouse β1.

Sonication:

Article Title: The plug domain of FepA, a TonB-dependent transport protein from Escherichia coli, binds its siderophore in the absence of the transmembrane barrel domain
Article Snippet: .. Cell pellets were resuspended in a 50% (wt/vol) mixture with a lysis buffer containing 1 mg/ml of chicken egg white lysozyme (Sigma), 50 mM Tris (pH 8.0), protease inhibitor mixture (one tablet per 100 ml of buffer, Boehringer Mannheim), 50 mM NaCl, and 6% glycerol, then sonicated while chilled on wet ice to lyse them. .. After lysis, 40 mg of protamine sulfate (Sigma) dissolved in water was added to precipitate nucleic acids.

Western Blot:

Article Title: The Influence of EGFR Inactivation on the Radiation Response in High Grade Glioma
Article Snippet: .. Western Blotting Cells were lysed in lysis buffer (150 mM NaCl, 20 mM Tris–HCl (pH 7.5), 1% NP40, 1 mM EDTA, 1 mM EGTA, 1 mM sodium orthovanadate, and protease inhibitor mixture (Boehringer Mannheim Co., Barcelona, Spain) at 4 °C. ..

Lysis:

Article Title: Structural and Functional Analysis of a Novel Coiled-Coil Protein Involved in Ypt6 GTPase-regulated Protein Transport in Yeast
Article Snippet: .. Thirty OD600 units of cells were collected and suspended in ice-cold lysis buffer (0.2 M Tris-Cl, pH 8.0, 6 mM MgCl2 , 1 mM EGTA, 1 mM DTT) containing 4 mM Pefabloc (Boehringer Mannheim) and 1/25 vol of protease inhibitor mixture (Complete TM, Boehringer Mannheim). ..

Article Title: The plug domain of FepA, a TonB-dependent transport protein from Escherichia coli, binds its siderophore in the absence of the transmembrane barrel domain
Article Snippet: .. Cell pellets were resuspended in a 50% (wt/vol) mixture with a lysis buffer containing 1 mg/ml of chicken egg white lysozyme (Sigma), 50 mM Tris (pH 8.0), protease inhibitor mixture (one tablet per 100 ml of buffer, Boehringer Mannheim), 50 mM NaCl, and 6% glycerol, then sonicated while chilled on wet ice to lyse them. .. After lysis, 40 mg of protamine sulfate (Sigma) dissolved in water was added to precipitate nucleic acids.

Article Title: The Influence of EGFR Inactivation on the Radiation Response in High Grade Glioma
Article Snippet: .. Western Blotting Cells were lysed in lysis buffer (150 mM NaCl, 20 mM Tris–HCl (pH 7.5), 1% NP40, 1 mM EDTA, 1 mM EGTA, 1 mM sodium orthovanadate, and protease inhibitor mixture (Boehringer Mannheim Co., Barcelona, Spain) at 4 °C. ..

Article Title: Role of the cytoplasmic tyrosines of ?1A integrins in transformation by v-src
Article Snippet: .. Briefly, cells were incubated on ice in lysis buffer containing 100 mM sodium chloride, 2 mM EDTA, 1 mM sodium orthovanadate, 0.5 mM sodium molybdate, protease inhibitor mixture (Boehringer Mannheim), 10 mM Tris⋅HCl (pH 7.6), and 0.5% (wt/vol) 3-[(3-cholamidopropyl)dimethylammonio]-1-peopanesulfonate. .. After centrifugation, the supernatants were precleared with agarose beads coupled with antibodies to rat IgG and IgM (American Qualex, La Mirada, CA) and subsequently incubated with rat MB1.2 antibody against mouse β1.

Injection:

Article Title: Ca2+ permeability and Na+ conductance in cellular toxicity caused by hyperactive DEG/ENaC channels
Article Snippet: .. Three days after injection, 30 oocytes were resuspended in 0.3 M sucrose plus 10 mM sodium phosphate (pH 7.4) containing a protease inhibitor mixture (Complete; Boehringer-Mannheim), and homogenized with 20 strokes in a glass homogeneizer. .. Pelleted membranes were then resuspended in solubilization buffer containing (in mM): 150 NaCl, 50 Tris·HCl, 1 EDTA, and 1% Triton X-100; pH 7.5 plus protease inhibitor mixture and subjected to SDS-PAGE ( ).

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    Boehringer Mannheim protease inhibitor mixture tablets
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    https://www.bioz.com/result/protease inhibitor mixture tablets/product/Boehringer Mannheim
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