protease inhibitor mix  (Bio-Rad)

 
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  • 99
    Name:
    Protease
    Description:
    1 3 ml reagent for use with PV92 PCR Informatics kit 1662100EDU for extracting DNA from hair follicles education use only
    Catalog Number:
    1662003EDU
    Price:
    None
    Category:
    PV92 PCR Informatics Kit
    Buy from Supplier


    Structured Review

    Bio-Rad protease inhibitor mix
    Protease
    1 3 ml reagent for use with PV92 PCR Informatics kit 1662100EDU for extracting DNA from hair follicles education use only
    https://www.bioz.com/result/protease inhibitor mix/product/Bio-Rad
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor mix - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Phage, Protease, Peptide: A novel trifecta enabling multiplex detection of viable bacterial pathogens
    Article Snippet: .. The TEV protease cassette was amplified with the iProof high-fidelity PCR kit (Bio-Rad Laboratories, Hercules, CA) using standard M13 forward and reverse primers. .. The PCR product was purified using QIAquick PCR Purification Kit (Qiagen, Valencia, CA), and then digested with restriction enzymes Eco RI and Hin dIII, both purchased from New England Biolabs (Ipswich, MA).

    Solubility:

    Article Title: Screening Fusion Tags for Improved Recombinant Protein Expression in E. coli with the Expresso® Solubility and Expression Screening System
    Article Snippet: .. *SelecTEV Protease, 10 U/µL *20× SelecTEV Protease buffer *100 mM DTT * Included in Expresso Solubility and Expression Screening System kits, or available separately (Lucigen) Purified fusion protein from Basic Protocol 3 SDS-PAGE gel (e.g. BioRad MiniProtean TGX, 4–20%) SDS-PAGE gel running buffer SDS-PAGE sample loading buffer ..

    Purification:

    Article Title: The Expression of Soluble and Active Recombinant Haemophilus influenzae IgA1 Protease in E. coli
    Article Snippet: .. The activity of purified IgA1 protease was analyzed with an Experion automated electrophoresis system (Bio-Rad). .. Eight μ L of purified IgA1 (1600 ng/μ L) was added to a PCR tube containing 1 μ L of IgA1 protease sample, and the resulting mixture was incubated in a heat block at 37°C for 1 min.

    Article Title: Screening Fusion Tags for Improved Recombinant Protein Expression in E. coli with the Expresso® Solubility and Expression Screening System
    Article Snippet: .. *SelecTEV Protease, 10 U/µL *20× SelecTEV Protease buffer *100 mM DTT * Included in Expresso Solubility and Expression Screening System kits, or available separately (Lucigen) Purified fusion protein from Basic Protocol 3 SDS-PAGE gel (e.g. BioRad MiniProtean TGX, 4–20%) SDS-PAGE gel running buffer SDS-PAGE sample loading buffer ..

    Electrophoresis:

    Article Title: The Expression of Soluble and Active Recombinant Haemophilus influenzae IgA1 Protease in E. coli
    Article Snippet: .. The activity of purified IgA1 protease was analyzed with an Experion automated electrophoresis system (Bio-Rad). .. Eight μ L of purified IgA1 (1600 ng/μ L) was added to a PCR tube containing 1 μ L of IgA1 protease sample, and the resulting mixture was incubated in a heat block at 37°C for 1 min.

    Activity Assay:

    Article Title: The Expression of Soluble and Active Recombinant Haemophilus influenzae IgA1 Protease in E. coli
    Article Snippet: .. The activity of purified IgA1 protease was analyzed with an Experion automated electrophoresis system (Bio-Rad). .. Eight μ L of purified IgA1 (1600 ng/μ L) was added to a PCR tube containing 1 μ L of IgA1 protease sample, and the resulting mixture was incubated in a heat block at 37°C for 1 min.

    Expressing:

    Article Title: Superior Therapeutic Index in Lymphoma Therapy: CD30+ CD34+ Hematopoietic Stem Cells Resist a Chimeric Antigen Receptor T-cell Attack
    Article Snippet: .. Expression of the granzyme B inactivating serine protease PI-9/Sp6 was monitored by intracellular cell staining utilizing the anti-PI-9 mAb (clone 7D8; AbD Serotec, Oxford, UK) and the Intrashure intracellular staining kit (BD Bioscience) according the manufacturer's recommendations. ..

    Article Title: Screening Fusion Tags for Improved Recombinant Protein Expression in E. coli with the Expresso® Solubility and Expression Screening System
    Article Snippet: .. *SelecTEV Protease, 10 U/µL *20× SelecTEV Protease buffer *100 mM DTT * Included in Expresso Solubility and Expression Screening System kits, or available separately (Lucigen) Purified fusion protein from Basic Protocol 3 SDS-PAGE gel (e.g. BioRad MiniProtean TGX, 4–20%) SDS-PAGE gel running buffer SDS-PAGE sample loading buffer ..

    Polymerase Chain Reaction:

    Article Title: Phage, Protease, Peptide: A novel trifecta enabling multiplex detection of viable bacterial pathogens
    Article Snippet: .. The TEV protease cassette was amplified with the iProof high-fidelity PCR kit (Bio-Rad Laboratories, Hercules, CA) using standard M13 forward and reverse primers. .. The PCR product was purified using QIAquick PCR Purification Kit (Qiagen, Valencia, CA), and then digested with restriction enzymes Eco RI and Hin dIII, both purchased from New England Biolabs (Ipswich, MA).

    Staining:

    Article Title: Superior Therapeutic Index in Lymphoma Therapy: CD30+ CD34+ Hematopoietic Stem Cells Resist a Chimeric Antigen Receptor T-cell Attack
    Article Snippet: .. Expression of the granzyme B inactivating serine protease PI-9/Sp6 was monitored by intracellular cell staining utilizing the anti-PI-9 mAb (clone 7D8; AbD Serotec, Oxford, UK) and the Intrashure intracellular staining kit (BD Bioscience) according the manufacturer's recommendations. ..

    SPR Assay:

    Article Title: Existing drugs as broad-spectrum and potent inhibitors for Zika virus by targeting NS2B-NS3 interaction
    Article Snippet: .. Affinity and kinetic analyses of the interactions between each drug and the His-NS3 protease domain were determined using a ProteOn XPR36 SPR instrument (Bio-Rad) at 25 °C. .. Refolded His-NS3 was immobilized (∼15 000 RU) onto a ProteOn GLH sensor chip (Bio-Rad).

    SDS Page:

    Article Title: Screening Fusion Tags for Improved Recombinant Protein Expression in E. coli with the Expresso® Solubility and Expression Screening System
    Article Snippet: .. *SelecTEV Protease, 10 U/µL *20× SelecTEV Protease buffer *100 mM DTT * Included in Expresso Solubility and Expression Screening System kits, or available separately (Lucigen) Purified fusion protein from Basic Protocol 3 SDS-PAGE gel (e.g. BioRad MiniProtean TGX, 4–20%) SDS-PAGE gel running buffer SDS-PAGE sample loading buffer ..

    Software:

    Article Title: Analysis of Prion Strains by PrPSc Profiling in Sporadic Creutzfeldt-Jakob DiseaseToward a Better Understanding of Human Prion Diseases
    Article Snippet: .. Cases were typed according to the size of the protease-resistant unglycosylated PrP fragment [ , ], and relative quantification of the signal was performed employing Quantity One software (Bio-Rad). .. To quantify PrPSc , serial dilutions of a standard sCJD brain homogenate (33, 16.5, and 8.25 μg of protein) were used on each Western blot to generate a standard curve.

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