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Nacalai protease inhibitor cocktail
Protease Inhibitor Cocktail, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protease inhibitor cocktail/product/Nacalai
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
protease inhibitor cocktail - by Bioz Stars, 2021-03
86/100 stars

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Protease Inhibitor:

Article Title: Staphylococcus epidermidis Esp Degrades Specific Proteins Associated with Staphylococcus aureus Biofilm Formation and Host-Pathogen Interaction
Article Snippet: .. Biofilms of various S. aureus strains formed in the test dish were detached by mixing with washing buffer composed of 10 mM Tris-HCl (pH 8.0) and protease inhibitor cocktail (Nacalai) and transferred into a test tube. ..

Article Title: Eucalyptus oil reduces allergic reactions and suppresses mast cell degranulation by downregulating IgE-FcεRI signalling
Article Snippet: Histamine concentrations in cell culture supernatants were measured at 30 min after stimulation using an ELISA kit (Cayman Chemical) according to the manufacturer’s instructions. .. Immunoblot analysis BMMCs were washed and lysed with iced lysis buffer (1% Triton X-100, 50 mM Tris–HCl [pH 7.5], 150 mM NaCl, protease inhibitor cocktail and phosphatase inhibitor cocktail; Nacalai Tesque) . .. The lysates were subjected to SDS-PAGE and immunoblotting.

Article Title: TRPC3-GEF-H1 axis mediates pressure overload-induced cardiac fibrosis
Article Snippet: E. Coli were then sonicated and lysed in lysis buffer containing 20 mM HEPES (pH 7.4), 150 mM NaCl, 5 mM MgCl2 , 1% (v/v) triton X-100, 1 mM dithiothreitol (DTT), 0.2 μM phenylmethylsulfonyl fluoride, 0.2 μg ml−1 aprotinin and 0.2 μg ml−1 leupeptin, and the GST-RBD was purified from the lysate by incubation with glutathione-Sepharose 4B beads (GE Healthcare) at 4 °C for 1 h. Active RhoA-pull-down experiments were carried out as described previously . .. Hearts and cardiac cells were homogenized and lysed in RBD buffer containing 50 mM Tris-HCl (pH 7.6), 500 mM NaCl, 10 mM MgCl2 , 1% (v/v) Triton X-100, 0.1% (w/v) SDS, 0.5% (w/v) deoxycholate, 50 mM NaF, 2 mM Na3 VO4 and a protease inhibitor cocktail (Nacalai). ..

Article Title: Staurosporine synergistically potentiates the deoxycholate‐mediated induction of COX‐2 expression
Article Snippet: A 100‐mmol/L sodium deoxycholate (Sigma‐Aldrich) solution was prepared in sterile water. .. The protease inhibitor cocktail was purchased from Nacalai Tesque (Kyoto, Japan). .. The rabbit anti‐COX‐2 polyclonal antibody and the mouse anti‐COX‐2 monoclonal antibody were purchased from Cell Signaling Technology and Cayman Chemical, respectively.

Article Title: Leukotriene B4 receptor type 2 protects against pneumolysin-dependent acute lung injury
Article Snippet: Briefly, the PLY plasmid was introduced into E. coli strain M15[pREP4] (QIAGEN), and the bacteria were cultured at 30 °C for 18 h. IPTG was then added to a final concentration of 0.1 mM to induce PLY expression. .. After 5 h of induction, cells were collected and lysed with X Tractor buffer® (Clontech) containing 2 unit/mL DNase I (Roche) and a protease inhibitor cocktail (nacalai). .. The lysate was centrifuged at 10,000 × g for 20 min, and the supernatant was collected and applied to TALON® metal affinity resin (Clontech).

Article Title: TRPC3-Nox2 complex mediates doxorubicin-induced myocardial atrophy
Article Snippet: .. Mouse hearts were homogenized in RIPA buffer containing 0.1% SDS, 0.5% sodium deoxycholate, 1% NP-40, 150 mM NaCl, 50 mM Tris-HCl (pH 7.4), and protease inhibitor cocktail (Nacalai). .. Samples (10 μg) were then fractionated by SDS-PAGE and transferred onto PVDF membranes (Millipore).

Lysis:

Article Title: Eucalyptus oil reduces allergic reactions and suppresses mast cell degranulation by downregulating IgE-FcεRI signalling
Article Snippet: Histamine concentrations in cell culture supernatants were measured at 30 min after stimulation using an ELISA kit (Cayman Chemical) according to the manufacturer’s instructions. .. Immunoblot analysis BMMCs were washed and lysed with iced lysis buffer (1% Triton X-100, 50 mM Tris–HCl [pH 7.5], 150 mM NaCl, protease inhibitor cocktail and phosphatase inhibitor cocktail; Nacalai Tesque) . .. The lysates were subjected to SDS-PAGE and immunoblotting.

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    Nacalai protease inhibitor cocktail
    Protease Inhibitor Cocktail, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor cocktail/product/Nacalai
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor cocktail - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Nacalai phosphatase inhibitor cocktail
    Phosphatase Inhibitor Cocktail, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase inhibitor cocktail/product/Nacalai
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitor cocktail - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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