protease inhibitor cocktail roche  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Protease Inhibitor Cocktail
    Description:

    Catalog Number:
    p8340
    Price:
    None
    Applications:
    Protease Inhibitor Cocktail has been used-. as a buffer component during sonication of GFP (green fluorescent protein)-huntingtin-transfected HEK 293 cells in GST (glutathione S-transferase) pull down assay. as a component of lysis buffer. as a component of radioimmunoprecipitation assay buffer (RIPA)
    Buy from Supplier


    Structured Review

    Millipore protease inhibitor cocktail roche

    https://www.bioz.com/result/protease inhibitor cocktail roche/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor cocktail roche - by Bioz Stars, 2020-04
    99/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: G-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A
    Article Snippet: Two wells with 293T cells were washed in PBS and lysed by sonication in immunoprecipitation buffer (150 mM NaCl, 50 mM Tris pH 7.5, 0.5% Triton X-100, 1 × protease inhibitor cocktail (Sigma P8340), 1 mM PMSF (Sigma P7626) and 1 mM Na3 VO4 (Sigma S6508)). .. The lysates were cleared by centrifugation and adjusted to equal protein concentration (between 2.5 - 3.0 μg protein/μl) by the Bradford method (BioRad 500-0006).

    Article Title: Phosphorylation and Proteolytic Cleavage of Gag Proteins in Budded Simian Immunodeficiency Virus
    Article Snippet: Radiolabeled virus was purified by centrifugation at 133,000 × g for 2 h at 4°C through a cushion of 20% sucrose in TNE buffer (20 mM Tris [pH 8.0], 150 mM NaCl, and 2 mM EDTA). .. Virus pellets were lysed in modified radioimmunoprecipitation assay (RIPA) buffer (phosphate-buffered saline with 1% [vol/vol] Igepal [NP-40], 0.5% [wt/vol] sodium deoxycholate, 0.1% [wt/vol] sodium dodecyl sulfate [SDS], 1 mM sodium orthovanadate, and protease inhibitor cocktail III [Calbiochem, San Diego, Calif.]).

    Immunocytochemistry:

    Article Title: The CaVβ Subunit Protects the I-II Loop of the Voltage-gated Calcium Channel CaV2.2 from Proteasomal Degradation but Not Oligoubiquitination *
    Article Snippet: For immunocytochemistry and co-immunoprecipitation experiments, transfections were carried out using PolyJet (SignaGen) according to the manufacturer's instructions using equal ratios of constructs unless otherwise stated. .. When included, the protease inhibitor MG132 (Calbiochem) was added at a concentration of 4 μm 24 h after transfection, and the cells were harvested or fixed a further 16–18 h afterward.

    Stable Transfection:

    Article Title: The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin
    Article Snippet: Cellular treatment with protease inhibitors MDCK cells stably expressing uromodulin were grown on coverslip. .. After 24 h, the medium was replaced with Optimem supplemented with 0.1% protease inhibitor cocktail (PIC) (P8340, Sigma-Aldrich, Saint Louis, MO), 0.1% DMSO, 0.1 mM AEBSF, 0.08 μM aprotinin, 1.4 μM E64, 4 μM bestatin, 2 μM leupeptin and 1.5 μM pepstatin A (Sigma-Aldrich).

    Article Title: Mutations associated with Charcot-Marie-Tooth disease cause SIMPLE protein mislocalization and degradation by the proteasome and aggresome-autophagy pathways
    Article Snippet: .. Stably transfected HEK293 cells expressing WT or mutant SIMPLE were incubated for 24 hours at 37°C with protease inhibitor MG132 (20 μM, Sigma), lysosome inhibitor NH4 Cl (50 mM, Sigma), lysosome inhibitor chloroquine (100 μM, Sigma), autophagy inhibitor 3-MA (10 mM, Sigma), autophagy activator rapamycin (100 nM, Sigma) or vehicle (0.1% DMSO) as described previously ( ). ..

    Article Title: Role of the carboxyl terminal di-leucine in phosphorylation and internalization of C5a receptor
    Article Snippet: Stably transfected CHO cells were placed in fresh complete medium for 15 min, and then stimulated with 50 nM C5a for 10 min (pulse). .. After the experiment, cells were briefly washed with ice-cold PBS, collected in PBS with protease inhibitor cocktail III (Calbiochem) and sonicated briefly.

    Construct:

    Article Title: The CaVβ Subunit Protects the I-II Loop of the Voltage-gated Calcium Channel CaV2.2 from Proteasomal Degradation but Not Oligoubiquitination *
    Article Snippet: For immunocytochemistry and co-immunoprecipitation experiments, transfections were carried out using PolyJet (SignaGen) according to the manufacturer's instructions using equal ratios of constructs unless otherwise stated. .. When included, the protease inhibitor MG132 (Calbiochem) was added at a concentration of 4 μm 24 h after transfection, and the cells were harvested or fixed a further 16–18 h afterward.

    Incubation:

    Article Title: G-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A
    Article Snippet: Two wells with 293T cells were washed in PBS and lysed by sonication in immunoprecipitation buffer (150 mM NaCl, 50 mM Tris pH 7.5, 0.5% Triton X-100, 1 × protease inhibitor cocktail (Sigma P8340), 1 mM PMSF (Sigma P7626) and 1 mM Na3 VO4 (Sigma S6508)). .. The lysates were precleared with Dynabeads Protein G (Invitrogen 100-04D, 1:10) and incubated over night with anti-GPKOW (0.8 μg, Atlas Antibodies HPA001894).

    Article Title: Phosphorylation and Proteolytic Cleavage of Gag Proteins in Budded Simian Immunodeficiency Virus
    Article Snippet: The medium was replaced with fresh phosphate-free DMEM containing [32 P]orthophosphate (Perkin-Elmer Life and Analytical Sciences, Inc., Boston, Mass.) at ∼1.0 mCi/ml, and cells were incubated for 2 h at 37°C. .. Virus pellets were lysed in modified radioimmunoprecipitation assay (RIPA) buffer (phosphate-buffered saline with 1% [vol/vol] Igepal [NP-40], 0.5% [wt/vol] sodium deoxycholate, 0.1% [wt/vol] sodium dodecyl sulfate [SDS], 1 mM sodium orthovanadate, and protease inhibitor cocktail III [Calbiochem, San Diego, Calif.]).

    Article Title: The CaVβ Subunit Protects the I-II Loop of the Voltage-gated Calcium Channel CaV2.2 from Proteasomal Degradation but Not Oligoubiquitination *
    Article Snippet: When included, the protease inhibitor MG132 (Calbiochem) was added at a concentration of 4 μm 24 h after transfection, and the cells were harvested or fixed a further 16–18 h afterward. .. At the concentration of MG132 used (4 μm ) and the time of incubation (16–18 h), MG132 was found to be effective at inhibiting proteasomal degradation without being detrimental to cell survival.

    Article Title: Mutations associated with Charcot-Marie-Tooth disease cause SIMPLE protein mislocalization and degradation by the proteasome and aggresome-autophagy pathways
    Article Snippet: .. Stably transfected HEK293 cells expressing WT or mutant SIMPLE were incubated for 24 hours at 37°C with protease inhibitor MG132 (20 μM, Sigma), lysosome inhibitor NH4 Cl (50 mM, Sigma), lysosome inhibitor chloroquine (100 μM, Sigma), autophagy inhibitor 3-MA (10 mM, Sigma), autophagy activator rapamycin (100 nM, Sigma) or vehicle (0.1% DMSO) as described previously ( ). ..

    Article Title: The C-Terminal Extension Unique to the Long Isoform of the Shelterin Component TIN2 Enhances Its Interaction with TRF2 in a Phosphorylation- and Dyskeratosis Congenita Cluster-Dependent Fashion
    Article Snippet: .. Cells were resuspended in ice-cold lysis buffer (50 mM Tris-HCl at pH 7.5, 1 mM EDTA, 400 mM NaCl, 1% Triton X-100, 0.1% SDS, 1 mM dithiothreitol [DTT], 1 mM phenylmethylsulfonyl fluoride [PMSF], and 1× protease inhibitor cocktail III [Calbiochem]) and incubated for 10 min on ice prior to addition of an equal amount of ice-cold water. .. Protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce).

    Cell Culture:

    Article Title: The CaVβ Subunit Protects the I-II Loop of the Voltage-gated Calcium Channel CaV2.2 from Proteasomal Degradation but Not Oligoubiquitination *
    Article Snippet: Paragraph title: Cell Culture and Transfection ... When included, the protease inhibitor MG132 (Calbiochem) was added at a concentration of 4 μm 24 h after transfection, and the cells were harvested or fixed a further 16–18 h afterward.

    Article Title: Metalloproteinase-mediated Shedding of Integrin \u03b22 Promotes Macrophage Efflux from Inflammatory Sites
    Article Snippet: Paragraph title: Cell Culture of Mouse Macrophages ... The metalloproteinase inhibitor GM6001 (Elastin Products; 50 μ m in DMSO) or a mixture of metalloproteinase-sparing protease inhibitors (Sigma P8340; containing 4-(2-aminoethyl)benzenesulfonyl fluoride, aprotinin, leupeptin, bestatin, pepstatin A, and E-64) were added in some experiments.

    Article Title: SUMO-specific protease 2 (SENP2) functions as a tumor suppressor in osteosarcoma via SOX9 degradation
    Article Snippet: Paragraph title: Samples, cell lines, cell culture and transfection ... After 36 h, a total of 20 µM protease inhibitor MG132 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) were added for additional 4 h.

    Article Title: MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation
    Article Snippet: Paragraph title: Reagents and cell culture ... MMP13 inhibitors WAY170523 (Tocris Bioscience), CL82198 (Cayman) and SC205756 (Santa Cruz Biotechnology), FGF/PDGF/VEGF RTK inhibitor 341610 (Merk Millipore), PI3 kinase inhibitors LY294002 (Sigma Aldrich), eIF4E/eIF4G interaction inhibitor 4EGI1 (Selleck), protease inhibitor MG132 (Sigma), the transcriptional inhibitor actinomycin D (ActD, Sigma) and protein biosynthesis inhibitor cycloheximide (CHX, Sigma) were dissolved in dimethyl sulphoxide (DMSO) and lysosomal inhibitor chloroquine (CQ, Sigma) was dissolved in sterilized double-distilled H2 O.

    Expressing:

    Article Title: The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin
    Article Snippet: Cellular treatment with protease inhibitors MDCK cells stably expressing uromodulin were grown on coverslip. .. After 24 h, the medium was replaced with Optimem supplemented with 0.1% protease inhibitor cocktail (PIC) (P8340, Sigma-Aldrich, Saint Louis, MO), 0.1% DMSO, 0.1 mM AEBSF, 0.08 μM aprotinin, 1.4 μM E64, 4 μM bestatin, 2 μM leupeptin and 1.5 μM pepstatin A (Sigma-Aldrich).

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
    Article Snippet: Co-immunoprecipitation Uninfected or E. chaffeensis -infected THP-1 cells at 2 d p.i. were lysed in modified lysis buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 1% NP40 [USB, 19638], 5% glycerol, and 1% protease inhibitor cocktail III [Calbiochem, 539134]) for 15 min and immunoprecipitated for 2 h with rabbit anti-Etf-1, -BECN1, or -PIK3C3, or mouse anti-RAB5 IgG that was cross-linked to protein A/G-magnetic beads (Pierce, 88805). .. HEK293 cells expressing codon-optimized Etf-1-HA and GFP-RAB5 (WT, DN, or CA) or GFP control were lysed in modified lysis buffer as described above.

    Article Title: Mutations associated with Charcot-Marie-Tooth disease cause SIMPLE protein mislocalization and degradation by the proteasome and aggresome-autophagy pathways
    Article Snippet: .. Stably transfected HEK293 cells expressing WT or mutant SIMPLE were incubated for 24 hours at 37°C with protease inhibitor MG132 (20 μM, Sigma), lysosome inhibitor NH4 Cl (50 mM, Sigma), lysosome inhibitor chloroquine (100 μM, Sigma), autophagy inhibitor 3-MA (10 mM, Sigma), autophagy activator rapamycin (100 nM, Sigma) or vehicle (0.1% DMSO) as described previously ( ). ..

    BIA-KA:

    Article Title: Parkin Protects Against Misfolded SOD1 Toxicity by Promoting Its Aggresome Formation and Autophagic Clearance
    Article Snippet: For measurement of steady-state protein levels, cells were either untreated or treated for 24 h with protease inhibitor MG132 (20 μM, Sigma), lysosome inhibitor chloroquine (CQ, 100 μM, Sigma), autophagy inhibitor 3-methyl-adenine (3MA, 10 mM, Sigma), or 0.1 % dimethylsulfoxide (DMSO, Fisher) as a vehicle control. .. Cells were then lysed with 1.1 % SDS, and protein concentrations were determined using a bicinchoninic acid (BCA) Assay Kit (Pierce).

    Article Title: The C-Terminal Extension Unique to the Long Isoform of the Shelterin Component TIN2 Enhances Its Interaction with TRF2 in a Phosphorylation- and Dyskeratosis Congenita Cluster-Dependent Fashion
    Article Snippet: Cells were resuspended in ice-cold lysis buffer (50 mM Tris-HCl at pH 7.5, 1 mM EDTA, 400 mM NaCl, 1% Triton X-100, 0.1% SDS, 1 mM dithiothreitol [DTT], 1 mM phenylmethylsulfonyl fluoride [PMSF], and 1× protease inhibitor cocktail III [Calbiochem]) and incubated for 10 min on ice prior to addition of an equal amount of ice-cold water. .. Protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce).

    Modification:

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
    Article Snippet: .. Co-immunoprecipitation Uninfected or E. chaffeensis -infected THP-1 cells at 2 d p.i. were lysed in modified lysis buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 1% NP40 [USB, 19638], 5% glycerol, and 1% protease inhibitor cocktail III [Calbiochem, 539134]) for 15 min and immunoprecipitated for 2 h with rabbit anti-Etf-1, -BECN1, or -PIK3C3, or mouse anti-RAB5 IgG that was cross-linked to protein A/G-magnetic beads (Pierce, 88805). .. Normal rabbit (Santa Cruz Biotechnology, sc-2027) or mouse IgG (Santa Cruz Biotechnology, sc-2025) was used as a negative control for immunoprecipitation.

    Article Title: Phosphorylation and Proteolytic Cleavage of Gag Proteins in Budded Simian Immunodeficiency Virus
    Article Snippet: .. Virus pellets were lysed in modified radioimmunoprecipitation assay (RIPA) buffer (phosphate-buffered saline with 1% [vol/vol] Igepal [NP-40], 0.5% [wt/vol] sodium deoxycholate, 0.1% [wt/vol] sodium dodecyl sulfate [SDS], 1 mM sodium orthovanadate, and protease inhibitor cocktail III [Calbiochem, San Diego, Calif.]). .. Radiolabeled cells were washed once with ice-cold phosphate-buffered saline and lysed in RIPA buffer, and cell lysates were clarified by sonication and centrifugation.

    Article Title: The CaVβ Subunit Protects the I-II Loop of the Voltage-gated Calcium Channel CaV2.2 from Proteasomal Degradation but Not Oligoubiquitination *
    Article Snippet: Cell Culture and Transfection tsA-201 cells (European Collection of Authenticated Cell Cultures (ECACC)) were cultured in Dulbecco's modified Eagle's medium in the presence of 10% fetal bovine serum, penicillin, streptomycin, and 2% GlutaMAX (Invitrogen). .. When included, the protease inhibitor MG132 (Calbiochem) was added at a concentration of 4 μm 24 h after transfection, and the cells were harvested or fixed a further 16–18 h afterward.

    Western Blot:

    Article Title: Strategy and rationale for urine collection protocols employed in the NEPTUNE study
    Article Snippet: Cellular pellet proteins We also examined protein stability in cellular pellets for selected proteins using Western analysis, with and without the above protease inhibitors (Additional file : Figure S1). .. There was substantially better detection of the podocyte marker of interest PODXL in cellular pellets stored with protease inhibitors, and again found that Sigma protease inhibitor product may in fact be superior to the more expensive Roche protease inhibitor cocktail (Additional file : Figure S1).

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
    Article Snippet: Co-immunoprecipitation Uninfected or E. chaffeensis -infected THP-1 cells at 2 d p.i. were lysed in modified lysis buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 1% NP40 [USB, 19638], 5% glycerol, and 1% protease inhibitor cocktail III [Calbiochem, 539134]) for 15 min and immunoprecipitated for 2 h with rabbit anti-Etf-1, -BECN1, or -PIK3C3, or mouse anti-RAB5 IgG that was cross-linked to protein A/G-magnetic beads (Pierce, 88805). .. Bound proteins were eluted by 2× SDS sample buffer and subjected to western blotting.

    Article Title: The CaVβ Subunit Protects the I-II Loop of the Voltage-gated Calcium Channel CaV2.2 from Proteasomal Degradation but Not Oligoubiquitination *
    Article Snippet: When included, the protease inhibitor MG132 (Calbiochem) was added at a concentration of 4 μm 24 h after transfection, and the cells were harvested or fixed a further 16–18 h afterward. .. Total protein yields from transfections in the absence and presence of MG132 were measured (for Western blotting and co-immunoprecipitation analysis) and found to be similar.

    Article Title: Metabolic Alterations in a Slow-Paced Model of Pancreatic Cancer-Induced Wasting
    Article Snippet: Samples were lysed in 0.5 ml buffer A (50 mM Tris, 100 mM KCl, 5 mM MgCl2 , 1.8 mM ATP, and 1 mM EDTA (pH 7.2)), supplemented with protease inhibitor cocktail III (Calbiochem), 1 mM PMSF, and 250 mM NaF. .. A 50 μ l aliquot was sonicated and used for the measurement of protein content or Western blotting; the remaining part was stored at −80°C.

    Transfection:

    Article Title: The CaVβ Subunit Protects the I-II Loop of the Voltage-gated Calcium Channel CaV2.2 from Proteasomal Degradation but Not Oligoubiquitination *
    Article Snippet: .. When included, the protease inhibitor MG132 (Calbiochem) was added at a concentration of 4 μm 24 h after transfection, and the cells were harvested or fixed a further 16–18 h afterward. .. At the concentration of MG132 used (4 μm ) and the time of incubation (16–18 h), MG132 was found to be effective at inhibiting proteasomal degradation without being detrimental to cell survival.

    Article Title: SUMO-specific protease 2 (SENP2) functions as a tumor suppressor in osteosarcoma via SOX9 degradation
    Article Snippet: Paragraph title: Samples, cell lines, cell culture and transfection ... After 36 h, a total of 20 µM protease inhibitor MG132 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) were added for additional 4 h.

    Article Title: Mutations associated with Charcot-Marie-Tooth disease cause SIMPLE protein mislocalization and degradation by the proteasome and aggresome-autophagy pathways
    Article Snippet: .. Stably transfected HEK293 cells expressing WT or mutant SIMPLE were incubated for 24 hours at 37°C with protease inhibitor MG132 (20 μM, Sigma), lysosome inhibitor NH4 Cl (50 mM, Sigma), lysosome inhibitor chloroquine (100 μM, Sigma), autophagy inhibitor 3-MA (10 mM, Sigma), autophagy activator rapamycin (100 nM, Sigma) or vehicle (0.1% DMSO) as described previously ( ). ..

    Article Title: Role of the carboxyl terminal di-leucine in phosphorylation and internalization of C5a receptor
    Article Snippet: Stably transfected CHO cells were placed in fresh complete medium for 15 min, and then stimulated with 50 nM C5a for 10 min (pulse). .. After the experiment, cells were briefly washed with ice-cold PBS, collected in PBS with protease inhibitor cocktail III (Calbiochem) and sonicated briefly.

    Pulse Chase:

    Article Title: Role of the carboxyl terminal di-leucine in phosphorylation and internalization of C5a receptor
    Article Snippet: Paragraph title: Pulse-chase dephosphorylation assay ... After the experiment, cells were briefly washed with ice-cold PBS, collected in PBS with protease inhibitor cocktail III (Calbiochem) and sonicated briefly.

    Concentration Assay:

    Article Title: The CaVβ Subunit Protects the I-II Loop of the Voltage-gated Calcium Channel CaV2.2 from Proteasomal Degradation but Not Oligoubiquitination *
    Article Snippet: .. When included, the protease inhibitor MG132 (Calbiochem) was added at a concentration of 4 μm 24 h after transfection, and the cells were harvested or fixed a further 16–18 h afterward. .. At the concentration of MG132 used (4 μm ) and the time of incubation (16–18 h), MG132 was found to be effective at inhibiting proteasomal degradation without being detrimental to cell survival.

    Article Title: MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation
    Article Snippet: MMP13 inhibitors WAY170523 (Tocris Bioscience), CL82198 (Cayman) and SC205756 (Santa Cruz Biotechnology), FGF/PDGF/VEGF RTK inhibitor 341610 (Merk Millipore), PI3 kinase inhibitors LY294002 (Sigma Aldrich), eIF4E/eIF4G interaction inhibitor 4EGI1 (Selleck), protease inhibitor MG132 (Sigma), the transcriptional inhibitor actinomycin D (ActD, Sigma) and protein biosynthesis inhibitor cycloheximide (CHX, Sigma) were dissolved in dimethyl sulphoxide (DMSO) and lysosomal inhibitor chloroquine (CQ, Sigma) was dissolved in sterilized double-distilled H2 O. .. The ultimate concentration of DMSO was not more than 1:2000.

    Protease Inhibitor:

    Article Title: G-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A
    Article Snippet: .. Two wells with 293T cells were washed in PBS and lysed by sonication in immunoprecipitation buffer (150 mM NaCl, 50 mM Tris pH 7.5, 0.5% Triton X-100, 1 × protease inhibitor cocktail (Sigma P8340), 1 mM PMSF (Sigma P7626) and 1 mM Na3 VO4 (Sigma S6508)). .. The lysates were cleared by centrifugation and adjusted to equal protein concentration (between 2.5 - 3.0 μg protein/μl) by the Bradford method (BioRad 500-0006).

    Article Title: The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin
    Article Snippet: .. After 24 h, the medium was replaced with Optimem supplemented with 0.1% protease inhibitor cocktail (PIC) (P8340, Sigma-Aldrich, Saint Louis, MO), 0.1% DMSO, 0.1 mM AEBSF, 0.08 μM aprotinin, 1.4 μM E64, 4 μM bestatin, 2 μM leupeptin and 1.5 μM pepstatin A (Sigma-Aldrich). .. Immunofluorescence analysis was performed after 24 hr of treatment.

    Article Title: Strategy and rationale for urine collection protocols employed in the NEPTUNE study
    Article Snippet: .. There was substantially better detection of the podocyte marker of interest PODXL in cellular pellets stored with protease inhibitors, and again found that Sigma protease inhibitor product may in fact be superior to the more expensive Roche protease inhibitor cocktail (Additional file : Figure S1). .. Without the presence of protease inhibitor, the proteins we examined (podocalyxin, fibrocystin and smoothened) did not survive 12 months of storage (6 month representative Western shown), or a freeze/thaw cycle at −80 °C.

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
    Article Snippet: .. Co-immunoprecipitation Uninfected or E. chaffeensis -infected THP-1 cells at 2 d p.i. were lysed in modified lysis buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 1% NP40 [USB, 19638], 5% glycerol, and 1% protease inhibitor cocktail III [Calbiochem, 539134]) for 15 min and immunoprecipitated for 2 h with rabbit anti-Etf-1, -BECN1, or -PIK3C3, or mouse anti-RAB5 IgG that was cross-linked to protein A/G-magnetic beads (Pierce, 88805). .. Normal rabbit (Santa Cruz Biotechnology, sc-2027) or mouse IgG (Santa Cruz Biotechnology, sc-2025) was used as a negative control for immunoprecipitation.

    Article Title: Phosphorylation and Proteolytic Cleavage of Gag Proteins in Budded Simian Immunodeficiency Virus
    Article Snippet: .. Virus pellets were lysed in modified radioimmunoprecipitation assay (RIPA) buffer (phosphate-buffered saline with 1% [vol/vol] Igepal [NP-40], 0.5% [wt/vol] sodium deoxycholate, 0.1% [wt/vol] sodium dodecyl sulfate [SDS], 1 mM sodium orthovanadate, and protease inhibitor cocktail III [Calbiochem, San Diego, Calif.]). .. Radiolabeled cells were washed once with ice-cold phosphate-buffered saline and lysed in RIPA buffer, and cell lysates were clarified by sonication and centrifugation.

    Article Title: The CaVβ Subunit Protects the I-II Loop of the Voltage-gated Calcium Channel CaV2.2 from Proteasomal Degradation but Not Oligoubiquitination *
    Article Snippet: .. When included, the protease inhibitor MG132 (Calbiochem) was added at a concentration of 4 μm 24 h after transfection, and the cells were harvested or fixed a further 16–18 h afterward. .. At the concentration of MG132 used (4 μm ) and the time of incubation (16–18 h), MG132 was found to be effective at inhibiting proteasomal degradation without being detrimental to cell survival.

    Article Title: SUMO-specific protease 2 (SENP2) functions as a tumor suppressor in osteosarcoma via SOX9 degradation
    Article Snippet: .. After 36 h, a total of 20 µM protease inhibitor MG132 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) were added for additional 4 h. .. U2OS cells were cultured to 70–80% confluence in 10 cm dishes and co-transfected with Flag-SENP2 (4 µg) and/or HA-SOX9 (4 µg) and pcDNA 3.1 (4 µg) control plasmids as above described.

    Article Title: Mutations associated with Charcot-Marie-Tooth disease cause SIMPLE protein mislocalization and degradation by the proteasome and aggresome-autophagy pathways
    Article Snippet: .. Stably transfected HEK293 cells expressing WT or mutant SIMPLE were incubated for 24 hours at 37°C with protease inhibitor MG132 (20 μM, Sigma), lysosome inhibitor NH4 Cl (50 mM, Sigma), lysosome inhibitor chloroquine (100 μM, Sigma), autophagy inhibitor 3-MA (10 mM, Sigma), autophagy activator rapamycin (100 nM, Sigma) or vehicle (0.1% DMSO) as described previously ( ). ..

    Article Title: Parkin Protects Against Misfolded SOD1 Toxicity by Promoting Its Aggresome Formation and Autophagic Clearance
    Article Snippet: .. For measurement of steady-state protein levels, cells were either untreated or treated for 24 h with protease inhibitor MG132 (20 μM, Sigma), lysosome inhibitor chloroquine (CQ, 100 μM, Sigma), autophagy inhibitor 3-methyl-adenine (3MA, 10 mM, Sigma), or 0.1 % dimethylsulfoxide (DMSO, Fisher) as a vehicle control. .. Cells were then lysed with 1.1 % SDS, and protein concentrations were determined using a bicinchoninic acid (BCA) Assay Kit (Pierce).

    Article Title: Metabolic Alterations in a Slow-Paced Model of Pancreatic Cancer-Induced Wasting
    Article Snippet: .. Samples were lysed in 0.5 ml buffer A (50 mM Tris, 100 mM KCl, 5 mM MgCl2 , 1.8 mM ATP, and 1 mM EDTA (pH 7.2)), supplemented with protease inhibitor cocktail III (Calbiochem), 1 mM PMSF, and 250 mM NaF. ..

    Article Title: MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation
    Article Snippet: .. MMP13 inhibitors WAY170523 (Tocris Bioscience), CL82198 (Cayman) and SC205756 (Santa Cruz Biotechnology), FGF/PDGF/VEGF RTK inhibitor 341610 (Merk Millipore), PI3 kinase inhibitors LY294002 (Sigma Aldrich), eIF4E/eIF4G interaction inhibitor 4EGI1 (Selleck), protease inhibitor MG132 (Sigma), the transcriptional inhibitor actinomycin D (ActD, Sigma) and protein biosynthesis inhibitor cycloheximide (CHX, Sigma) were dissolved in dimethyl sulphoxide (DMSO) and lysosomal inhibitor chloroquine (CQ, Sigma) was dissolved in sterilized double-distilled H2 O. .. The ultimate concentration of DMSO was not more than 1:2000.

    Article Title: ER Stress-Induced Clearance of Misfolded GPI-Anchored Proteins via the Secretory Pathway
    Article Snippet: .. Drug Treatments Working concentrations are 0.1 μM thapsigargin, 0.5 mM dithiothreitol, 5 mM methyl-β-cyclodextrin, and 1 μM brefeldin A. Lysosomal protease inhibitors refer to 125 μM leupeptin + protease inhibitor cocktail (Sigma P8340) diluted 1:100. .. Microscopy All fixed and live-cell imaging, excluding FRAP, were performed using the Marianas spinning disk confocal system (Intelligent Imaging Innovations) attached to a Zeiss Observer.Z1 microscope (Carl Zeiss MicroImaging) and analyzed by Slidebook software.

    Article Title: The C-Terminal Extension Unique to the Long Isoform of the Shelterin Component TIN2 Enhances Its Interaction with TRF2 in a Phosphorylation- and Dyskeratosis Congenita Cluster-Dependent Fashion
    Article Snippet: .. Cells were resuspended in ice-cold lysis buffer (50 mM Tris-HCl at pH 7.5, 1 mM EDTA, 400 mM NaCl, 1% Triton X-100, 0.1% SDS, 1 mM dithiothreitol [DTT], 1 mM phenylmethylsulfonyl fluoride [PMSF], and 1× protease inhibitor cocktail III [Calbiochem]) and incubated for 10 min on ice prior to addition of an equal amount of ice-cold water. .. Protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce).

    Article Title: Role of the carboxyl terminal di-leucine in phosphorylation and internalization of C5a receptor
    Article Snippet: .. After the experiment, cells were briefly washed with ice-cold PBS, collected in PBS with protease inhibitor cocktail III (Calbiochem) and sonicated briefly. .. Protein samples were lysed in Laemmli sample buffer, heated at 60°C for 15 min, separated on 8% or 10% SDS-polyacrylamide gel, and transferred to a 0.45 μm nitrocellulose membrane using a semi-wet unit (Bio-Rad).

    Infection:

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
    Article Snippet: .. Co-immunoprecipitation Uninfected or E. chaffeensis -infected THP-1 cells at 2 d p.i. were lysed in modified lysis buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 1% NP40 [USB, 19638], 5% glycerol, and 1% protease inhibitor cocktail III [Calbiochem, 539134]) for 15 min and immunoprecipitated for 2 h with rabbit anti-Etf-1, -BECN1, or -PIK3C3, or mouse anti-RAB5 IgG that was cross-linked to protein A/G-magnetic beads (Pierce, 88805). .. Normal rabbit (Santa Cruz Biotechnology, sc-2027) or mouse IgG (Santa Cruz Biotechnology, sc-2025) was used as a negative control for immunoprecipitation.

    Protein Concentration:

    Article Title: G-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A
    Article Snippet: Two wells with 293T cells were washed in PBS and lysed by sonication in immunoprecipitation buffer (150 mM NaCl, 50 mM Tris pH 7.5, 0.5% Triton X-100, 1 × protease inhibitor cocktail (Sigma P8340), 1 mM PMSF (Sigma P7626) and 1 mM Na3 VO4 (Sigma S6508)). .. The lysates were cleared by centrifugation and adjusted to equal protein concentration (between 2.5 - 3.0 μg protein/μl) by the Bradford method (BioRad 500-0006).

    Article Title: The C-Terminal Extension Unique to the Long Isoform of the Shelterin Component TIN2 Enhances Its Interaction with TRF2 in a Phosphorylation- and Dyskeratosis Congenita Cluster-Dependent Fashion
    Article Snippet: Cells were resuspended in ice-cold lysis buffer (50 mM Tris-HCl at pH 7.5, 1 mM EDTA, 400 mM NaCl, 1% Triton X-100, 0.1% SDS, 1 mM dithiothreitol [DTT], 1 mM phenylmethylsulfonyl fluoride [PMSF], and 1× protease inhibitor cocktail III [Calbiochem]) and incubated for 10 min on ice prior to addition of an equal amount of ice-cold water. .. Protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce).

    Sonication:

    Article Title: G-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A
    Article Snippet: .. Two wells with 293T cells were washed in PBS and lysed by sonication in immunoprecipitation buffer (150 mM NaCl, 50 mM Tris pH 7.5, 0.5% Triton X-100, 1 × protease inhibitor cocktail (Sigma P8340), 1 mM PMSF (Sigma P7626) and 1 mM Na3 VO4 (Sigma S6508)). .. The lysates were cleared by centrifugation and adjusted to equal protein concentration (between 2.5 - 3.0 μg protein/μl) by the Bradford method (BioRad 500-0006).

    Article Title: Phosphorylation and Proteolytic Cleavage of Gag Proteins in Budded Simian Immunodeficiency Virus
    Article Snippet: Virus pellets were lysed in modified radioimmunoprecipitation assay (RIPA) buffer (phosphate-buffered saline with 1% [vol/vol] Igepal [NP-40], 0.5% [wt/vol] sodium deoxycholate, 0.1% [wt/vol] sodium dodecyl sulfate [SDS], 1 mM sodium orthovanadate, and protease inhibitor cocktail III [Calbiochem, San Diego, Calif.]). .. Radiolabeled cells were washed once with ice-cold phosphate-buffered saline and lysed in RIPA buffer, and cell lysates were clarified by sonication and centrifugation.

    Article Title: Metabolic Alterations in a Slow-Paced Model of Pancreatic Cancer-Induced Wasting
    Article Snippet: Samples were lysed in 0.5 ml buffer A (50 mM Tris, 100 mM KCl, 5 mM MgCl2 , 1.8 mM ATP, and 1 mM EDTA (pH 7.2)), supplemented with protease inhibitor cocktail III (Calbiochem), 1 mM PMSF, and 250 mM NaF. .. A 50 μ l aliquot was sonicated and used for the measurement of protein content or Western blotting; the remaining part was stored at −80°C.

    Article Title: Role of the carboxyl terminal di-leucine in phosphorylation and internalization of C5a receptor
    Article Snippet: .. After the experiment, cells were briefly washed with ice-cold PBS, collected in PBS with protease inhibitor cocktail III (Calbiochem) and sonicated briefly. .. Protein samples were lysed in Laemmli sample buffer, heated at 60°C for 15 min, separated on 8% or 10% SDS-polyacrylamide gel, and transferred to a 0.45 μm nitrocellulose membrane using a semi-wet unit (Bio-Rad).

    Immunofluorescence:

    Article Title: The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin
    Article Snippet: After 24 h, the medium was replaced with Optimem supplemented with 0.1% protease inhibitor cocktail (PIC) (P8340, Sigma-Aldrich, Saint Louis, MO), 0.1% DMSO, 0.1 mM AEBSF, 0.08 μM aprotinin, 1.4 μM E64, 4 μM bestatin, 2 μM leupeptin and 1.5 μM pepstatin A (Sigma-Aldrich). .. Immunofluorescence analysis was performed after 24 hr of treatment.

    Mutagenesis:

    Article Title: Mutations associated with Charcot-Marie-Tooth disease cause SIMPLE protein mislocalization and degradation by the proteasome and aggresome-autophagy pathways
    Article Snippet: .. Stably transfected HEK293 cells expressing WT or mutant SIMPLE were incubated for 24 hours at 37°C with protease inhibitor MG132 (20 μM, Sigma), lysosome inhibitor NH4 Cl (50 mM, Sigma), lysosome inhibitor chloroquine (100 μM, Sigma), autophagy inhibitor 3-MA (10 mM, Sigma), autophagy activator rapamycin (100 nM, Sigma) or vehicle (0.1% DMSO) as described previously ( ). ..

    Isolation:

    Article Title: Metabolic Alterations in a Slow-Paced Model of Pancreatic Cancer-Induced Wasting
    Article Snippet: Paragraph title: 2.12. Mitochondrial Isolation ... Samples were lysed in 0.5 ml buffer A (50 mM Tris, 100 mM KCl, 5 mM MgCl2 , 1.8 mM ATP, and 1 mM EDTA (pH 7.2)), supplemented with protease inhibitor cocktail III (Calbiochem), 1 mM PMSF, and 250 mM NaF.

    Labeling:

    Article Title: Phosphorylation and Proteolytic Cleavage of Gag Proteins in Budded Simian Immunodeficiency Virus
    Article Snippet: Paragraph title: Metabolic labeling and immunoprecipitation of Gag proteins. ... Virus pellets were lysed in modified radioimmunoprecipitation assay (RIPA) buffer (phosphate-buffered saline with 1% [vol/vol] Igepal [NP-40], 0.5% [wt/vol] sodium deoxycholate, 0.1% [wt/vol] sodium dodecyl sulfate [SDS], 1 mM sodium orthovanadate, and protease inhibitor cocktail III [Calbiochem, San Diego, Calif.]).

    Purification:

    Article Title: Phosphorylation and Proteolytic Cleavage of Gag Proteins in Budded Simian Immunodeficiency Virus
    Article Snippet: Radiolabeled virus was purified by centrifugation at 133,000 × g for 2 h at 4°C through a cushion of 20% sucrose in TNE buffer (20 mM Tris [pH 8.0], 150 mM NaCl, and 2 mM EDTA). .. Virus pellets were lysed in modified radioimmunoprecipitation assay (RIPA) buffer (phosphate-buffered saline with 1% [vol/vol] Igepal [NP-40], 0.5% [wt/vol] sodium deoxycholate, 0.1% [wt/vol] sodium dodecyl sulfate [SDS], 1 mM sodium orthovanadate, and protease inhibitor cocktail III [Calbiochem, San Diego, Calif.]).

    De-Phosphorylation Assay:

    Article Title: G-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A
    Article Snippet: Paragraph title: Dephosphorylation assay ... Two wells with 293T cells were washed in PBS and lysed by sonication in immunoprecipitation buffer (150 mM NaCl, 50 mM Tris pH 7.5, 0.5% Triton X-100, 1 × protease inhibitor cocktail (Sigma P8340), 1 mM PMSF (Sigma P7626) and 1 mM Na3 VO4 (Sigma S6508)).

    Article Title: Role of the carboxyl terminal di-leucine in phosphorylation and internalization of C5a receptor
    Article Snippet: Paragraph title: Pulse-chase dephosphorylation assay ... After the experiment, cells were briefly washed with ice-cold PBS, collected in PBS with protease inhibitor cocktail III (Calbiochem) and sonicated briefly.

    SDS Page:

    Article Title: Parkin Protects Against Misfolded SOD1 Toxicity by Promoting Its Aggresome Formation and Autophagic Clearance
    Article Snippet: For measurement of steady-state protein levels, cells were either untreated or treated for 24 h with protease inhibitor MG132 (20 μM, Sigma), lysosome inhibitor chloroquine (CQ, 100 μM, Sigma), autophagy inhibitor 3-methyl-adenine (3MA, 10 mM, Sigma), or 0.1 % dimethylsulfoxide (DMSO, Fisher) as a vehicle control. .. Equal amounts of total proteins from each lysate were subjected to SDS-PAGE and immunoblotting using the indicated antibodies.

    Negative Control:

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
    Article Snippet: Co-immunoprecipitation Uninfected or E. chaffeensis -infected THP-1 cells at 2 d p.i. were lysed in modified lysis buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 1% NP40 [USB, 19638], 5% glycerol, and 1% protease inhibitor cocktail III [Calbiochem, 539134]) for 15 min and immunoprecipitated for 2 h with rabbit anti-Etf-1, -BECN1, or -PIK3C3, or mouse anti-RAB5 IgG that was cross-linked to protein A/G-magnetic beads (Pierce, 88805). .. Normal rabbit (Santa Cruz Biotechnology, sc-2027) or mouse IgG (Santa Cruz Biotechnology, sc-2025) was used as a negative control for immunoprecipitation.

    Radio Immunoprecipitation:

    Article Title: Phosphorylation and Proteolytic Cleavage of Gag Proteins in Budded Simian Immunodeficiency Virus
    Article Snippet: .. Virus pellets were lysed in modified radioimmunoprecipitation assay (RIPA) buffer (phosphate-buffered saline with 1% [vol/vol] Igepal [NP-40], 0.5% [wt/vol] sodium deoxycholate, 0.1% [wt/vol] sodium dodecyl sulfate [SDS], 1 mM sodium orthovanadate, and protease inhibitor cocktail III [Calbiochem, San Diego, Calif.]). .. Radiolabeled cells were washed once with ice-cold phosphate-buffered saline and lysed in RIPA buffer, and cell lysates were clarified by sonication and centrifugation.

    Immunoprecipitation:

    Article Title: G-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A
    Article Snippet: .. Two wells with 293T cells were washed in PBS and lysed by sonication in immunoprecipitation buffer (150 mM NaCl, 50 mM Tris pH 7.5, 0.5% Triton X-100, 1 × protease inhibitor cocktail (Sigma P8340), 1 mM PMSF (Sigma P7626) and 1 mM Na3 VO4 (Sigma S6508)). .. The lysates were cleared by centrifugation and adjusted to equal protein concentration (between 2.5 - 3.0 μg protein/μl) by the Bradford method (BioRad 500-0006).

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
    Article Snippet: .. Co-immunoprecipitation Uninfected or E. chaffeensis -infected THP-1 cells at 2 d p.i. were lysed in modified lysis buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 1% NP40 [USB, 19638], 5% glycerol, and 1% protease inhibitor cocktail III [Calbiochem, 539134]) for 15 min and immunoprecipitated for 2 h with rabbit anti-Etf-1, -BECN1, or -PIK3C3, or mouse anti-RAB5 IgG that was cross-linked to protein A/G-magnetic beads (Pierce, 88805). .. Normal rabbit (Santa Cruz Biotechnology, sc-2027) or mouse IgG (Santa Cruz Biotechnology, sc-2025) was used as a negative control for immunoprecipitation.

    Article Title: Phosphorylation and Proteolytic Cleavage of Gag Proteins in Budded Simian Immunodeficiency Virus
    Article Snippet: Paragraph title: Metabolic labeling and immunoprecipitation of Gag proteins. ... Virus pellets were lysed in modified radioimmunoprecipitation assay (RIPA) buffer (phosphate-buffered saline with 1% [vol/vol] Igepal [NP-40], 0.5% [wt/vol] sodium deoxycholate, 0.1% [wt/vol] sodium dodecyl sulfate [SDS], 1 mM sodium orthovanadate, and protease inhibitor cocktail III [Calbiochem, San Diego, Calif.]).

    Marker:

    Article Title: Strategy and rationale for urine collection protocols employed in the NEPTUNE study
    Article Snippet: .. There was substantially better detection of the podocyte marker of interest PODXL in cellular pellets stored with protease inhibitors, and again found that Sigma protease inhibitor product may in fact be superior to the more expensive Roche protease inhibitor cocktail (Additional file : Figure S1). .. Without the presence of protease inhibitor, the proteins we examined (podocalyxin, fibrocystin and smoothened) did not survive 12 months of storage (6 month representative Western shown), or a freeze/thaw cycle at −80 °C.

    Lysis:

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
    Article Snippet: .. Co-immunoprecipitation Uninfected or E. chaffeensis -infected THP-1 cells at 2 d p.i. were lysed in modified lysis buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 1% NP40 [USB, 19638], 5% glycerol, and 1% protease inhibitor cocktail III [Calbiochem, 539134]) for 15 min and immunoprecipitated for 2 h with rabbit anti-Etf-1, -BECN1, or -PIK3C3, or mouse anti-RAB5 IgG that was cross-linked to protein A/G-magnetic beads (Pierce, 88805). .. Normal rabbit (Santa Cruz Biotechnology, sc-2027) or mouse IgG (Santa Cruz Biotechnology, sc-2025) was used as a negative control for immunoprecipitation.

    Article Title: Metalloproteinase-mediated Shedding of Integrin \u03b22 Promotes Macrophage Efflux from Inflammatory Sites
    Article Snippet: The metalloproteinase inhibitor GM6001 (Elastin Products; 50 μ m in DMSO) or a mixture of metalloproteinase-sparing protease inhibitors (Sigma P8340; containing 4-(2-aminoethyl)benzenesulfonyl fluoride, aprotinin, leupeptin, bestatin, pepstatin A, and E-64) were added in some experiments. .. Macrophage lysates were prepared in Nonidet P-40 lysis buffer.

    Article Title: The C-Terminal Extension Unique to the Long Isoform of the Shelterin Component TIN2 Enhances Its Interaction with TRF2 in a Phosphorylation- and Dyskeratosis Congenita Cluster-Dependent Fashion
    Article Snippet: .. Cells were resuspended in ice-cold lysis buffer (50 mM Tris-HCl at pH 7.5, 1 mM EDTA, 400 mM NaCl, 1% Triton X-100, 0.1% SDS, 1 mM dithiothreitol [DTT], 1 mM phenylmethylsulfonyl fluoride [PMSF], and 1× protease inhibitor cocktail III [Calbiochem]) and incubated for 10 min on ice prior to addition of an equal amount of ice-cold water. .. Protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore edta free protease inhibitor cocktail
    Edta Free Protease Inhibitor Cocktail, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta free protease inhibitor cocktail/product/Millipore
    Average 99 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    edta free protease inhibitor cocktail - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore phosphatase inhibitor cocktails
    Phosphatase Inhibitor Cocktails, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase inhibitor cocktails/product/Millipore
    Average 99 stars, based on 633 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitor cocktails - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results