protease inhibitor cocktail roche molecular biochemicals penzberg germany  (Roche)

 
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    Structured Review

    Roche protease inhibitor cocktail roche molecular biochemicals penzberg germany
    Protease Inhibitor Cocktail Roche Molecular Biochemicals Penzberg Germany, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor cocktail roche molecular biochemicals penzberg germany/product/Roche
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor cocktail roche molecular biochemicals penzberg germany - by Bioz Stars, 2020-04
    80/100 stars

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    Related Articles

    Protease Inhibitor:

    Article Title: Androgen receptor degraders overcome common resistance mechanisms developed during prostate cancer treatment
    Article Snippet: .. Whole-cell lysates collected from cells seeded at 1 × 106 cells per well of a 6 well plate (Becton, Dickinson and Company, Franklin Lakes, New Jersey) were lysed in RIPA-PIC buffer [150 mM sodium chloride, 1.0% Igepal CA-630 (Sigma-Aldrich), 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0, 1 × protease inhibitor cocktail (Roche Molecular Biochemicals; Penzberg, Germany)], scraped, and sonicated (Fisher Scientific; Hampton, NH; model FB-120 Sonic Dismembrator). .. Protein was quantified by BCA assay (Thermo-Fisher Scientific); 30 μg of protein were loaded per lane, separated by SDS-PAGE and transferred onto nitrocellulose membrane (GE Healthcare, Chicago, IL).

    Blocking Assay:

    Article Title: Androgen receptor degraders overcome common resistance mechanisms developed during prostate cancer treatment
    Article Snippet: Whole-cell lysates collected from cells seeded at 1 × 106 cells per well of a 6 well plate (Becton, Dickinson and Company, Franklin Lakes, New Jersey) were lysed in RIPA-PIC buffer [150 mM sodium chloride, 1.0% Igepal CA-630 (Sigma-Aldrich), 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0, 1 × protease inhibitor cocktail (Roche Molecular Biochemicals; Penzberg, Germany)], scraped, and sonicated (Fisher Scientific; Hampton, NH; model FB-120 Sonic Dismembrator). .. The membrane was incubated for 1 hour in blocking buffer [Tris-buffered saline, 0.1% Tween (TBS-T), 5% nonfat dry milk] followed by incubation overnight at 4 °C with the primary antibody.

    Incubation:

    Article Title: Androgen receptor degraders overcome common resistance mechanisms developed during prostate cancer treatment
    Article Snippet: Whole-cell lysates collected from cells seeded at 1 × 106 cells per well of a 6 well plate (Becton, Dickinson and Company, Franklin Lakes, New Jersey) were lysed in RIPA-PIC buffer [150 mM sodium chloride, 1.0% Igepal CA-630 (Sigma-Aldrich), 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0, 1 × protease inhibitor cocktail (Roche Molecular Biochemicals; Penzberg, Germany)], scraped, and sonicated (Fisher Scientific; Hampton, NH; model FB-120 Sonic Dismembrator). .. The membrane was incubated for 1 hour in blocking buffer [Tris-buffered saline, 0.1% Tween (TBS-T), 5% nonfat dry milk] followed by incubation overnight at 4 °C with the primary antibody.

    BIA-KA:

    Article Title: Androgen receptor degraders overcome common resistance mechanisms developed during prostate cancer treatment
    Article Snippet: Whole-cell lysates collected from cells seeded at 1 × 106 cells per well of a 6 well plate (Becton, Dickinson and Company, Franklin Lakes, New Jersey) were lysed in RIPA-PIC buffer [150 mM sodium chloride, 1.0% Igepal CA-630 (Sigma-Aldrich), 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0, 1 × protease inhibitor cocktail (Roche Molecular Biochemicals; Penzberg, Germany)], scraped, and sonicated (Fisher Scientific; Hampton, NH; model FB-120 Sonic Dismembrator). .. Protein was quantified by BCA assay (Thermo-Fisher Scientific); 30 μg of protein were loaded per lane, separated by SDS-PAGE and transferred onto nitrocellulose membrane (GE Healthcare, Chicago, IL).

    Sonication:

    Article Title: Androgen receptor degraders overcome common resistance mechanisms developed during prostate cancer treatment
    Article Snippet: .. Whole-cell lysates collected from cells seeded at 1 × 106 cells per well of a 6 well plate (Becton, Dickinson and Company, Franklin Lakes, New Jersey) were lysed in RIPA-PIC buffer [150 mM sodium chloride, 1.0% Igepal CA-630 (Sigma-Aldrich), 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0, 1 × protease inhibitor cocktail (Roche Molecular Biochemicals; Penzberg, Germany)], scraped, and sonicated (Fisher Scientific; Hampton, NH; model FB-120 Sonic Dismembrator). .. Protein was quantified by BCA assay (Thermo-Fisher Scientific); 30 μg of protein were loaded per lane, separated by SDS-PAGE and transferred onto nitrocellulose membrane (GE Healthcare, Chicago, IL).

    SDS Page:

    Article Title: Androgen receptor degraders overcome common resistance mechanisms developed during prostate cancer treatment
    Article Snippet: Whole-cell lysates collected from cells seeded at 1 × 106 cells per well of a 6 well plate (Becton, Dickinson and Company, Franklin Lakes, New Jersey) were lysed in RIPA-PIC buffer [150 mM sodium chloride, 1.0% Igepal CA-630 (Sigma-Aldrich), 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0, 1 × protease inhibitor cocktail (Roche Molecular Biochemicals; Penzberg, Germany)], scraped, and sonicated (Fisher Scientific; Hampton, NH; model FB-120 Sonic Dismembrator). .. Protein was quantified by BCA assay (Thermo-Fisher Scientific); 30 μg of protein were loaded per lane, separated by SDS-PAGE and transferred onto nitrocellulose membrane (GE Healthcare, Chicago, IL).

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    Roche protease inhibitor cocktail roche molecular biochemicals penzberg germany
    Protease Inhibitor Cocktail Roche Molecular Biochemicals Penzberg Germany, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor cocktail roche molecular biochemicals penzberg germany/product/Roche
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor cocktail roche molecular biochemicals penzberg germany - by Bioz Stars, 2020-04
    80/100 stars
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