protease inhibitor cocktail roche cat no 11836153001  (Roche)

 
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    Structured Review

    Roche protease inhibitor cocktail roche cat no 11836153001
    Protease Inhibitor Cocktail Roche Cat No 11836153001, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor cocktail roche cat no 11836153001/product/Roche
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor cocktail roche cat no 11836153001 - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    Sample Prep:

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: Paragraph title: 2.1. Sample preparation ... Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used.

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: .. 2.1 Sample preparation Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

    Protease Inhibitor:

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: .. Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: .. 2.1 Sample preparation Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

    Acid Assay:

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: .. Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: .. 2.1 Sample preparation Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

    Concentration Assay:

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: 2.1 Sample preparation Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

    BIA-KA:

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. Added 200 ul of BCA reagent and mixed thoroughly on the shaker.

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: 2.1 Sample preparation Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. Added 200 ul of BCA reagent and mixed thoroughly on the shaker.

    Sonication:

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: .. Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: .. 2.1 Sample preparation Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

    Lysis:

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: .. Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: .. 2.1 Sample preparation Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: .. Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

    Article Title: Data of phosphoproteomic analysis of non-functioning pituitary adenoma
    Article Snippet: .. 2.1 Sample preparation Tumors from twenty male patients of NFPAs have been collected and protein was extracted using lysis buffer 2%SDS, 1 mM sodiumorthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, protease inhibitor cocktail (Roche, Cat.No.11836153001), sonicated and centrifuged at 16,000g at 15 °C for 20 min. To measure the protein amount in lysates, Bicinchoninic acid assay (Pierce, Waltham, MA Cat #23225) was used. .. A series of dilutions (100 ug/ml to 2000 ug/ml) of known concentration were prepared from the Bovine Serum Albumin to make the standard curve.

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