# propidium iodide pi staining solutions (Nexcelom Bioscience)

## Structured Review

Propidium Iodide Pi Staining Solutions, supplied by Nexcelom Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/propidium iodide pi staining solutions/product/Nexcelom Bioscience

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

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# propidium iodide pi uptake (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

## Structured Review

Propidium Iodide Pi Uptake, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/propidium iodide pi uptake/product/ATCC

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

# propidium iodide pi (Danaher Inc)

Danaher Inc is a verified supplier

Danaher Inc manufactures this product

## Structured Review

Propidium Iodide Pi, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/propidium iodide pi/product/Danaher Inc

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

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# propidium iodide pi (Millipore)

## Structured Review

Propidium Iodide Pi, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/propidium iodide pi/product/Millipore

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

# propidium iodide pi (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

## Structured Review

Propidium Iodide Pi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/propidium iodide pi/product/Thermo Fisher

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

# nuclear markers propidium iodide pi (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

## Structured Review

Nuclear Markers Propidium Iodide Pi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/nuclear markers propidium iodide pi/product/Thermo Fisher

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

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# propidium iodide pi (Millipore)

## Structured Review

Propidium Iodide Pi, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/propidium iodide pi/product/Millipore

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

### 1) Product Images from "Integrative analysis of patient-derived tumoroids and ex vivo organoid modeling of ARID1A loss in bladder cancer reveals therapeutic molecular targets"

**Article Title: **Integrative analysis of patient-derived tumoroids and ex vivo organoid modeling of ARID1A loss in bladder cancer reveals therapeutic molecular targets

**Journal: **bioRxiv

**doi: **10.1101/2024.07.29.601702

**Figure Legend Snippet:**A) Bar graphs summarizing cell cycle distribution of ARID1A-expressing (PDT20, PDT36, PDT39) and ARID1A-deficient (PDT3, PDT30, and PDT37) tumoroids at baseline (untreated control, left), and after treatment with 32nM prexasertib (middle) or 50nM YM155 (right). Data are represented as mean ± SEM. B) Flow cytometry of ARID1A-expressing (PDT36) and ARID1A-deficient (PDT30) tumoroids treated with 32nM prexasertib or 50nM YM155 for four days. Gated on the single, live population followed by analysis of propidium iodide (cell cycle) and phosphorylated H2Ax (yH2Ax) to identify the yH2Ax+ fraction (red). C) Bar graphs depicting percentage of yH2Ax cells in ARID1A-expressing tumoroids (PDT20, PDT36, PDT39) and ARID1A-deficient tumoroids (PDT3, PDT30, PDT37), treated with 32nM prexasertib or 50nM YM155 for two days. (mean ± SEM, two-way ANOVA, *p<0.05, **p<0.005). D) Dose-response curves for ARID1A-expressing (blue) and ARID1A-deficient (orange) PDTs treated with CHEK1 -inhibitor prexasertib (left), and BIRC5-inhibitor YM155 (right). Cell viability was measured through CellTiter-Glo 3D. (technical triplicates of two independent experiments; mean ± SEM). E) Left: IC50 values estimated from non-linear fit of dose-response depicted in D. YM155 IC50 concentration could not be determined for PDT10.1 due to the line’s high resistance. Right: Area under the curve (AUC) of dose-response depicted in D. two-way ANOVA, *p<0.05, ** p<0.005. F) Fluorescence staining of ARID1A-expressing (PDT36) and ARID1A-deficient (PDT30) PDTs, treated with 32nM prexasertib or 50nM YM155 for two days. Tumoroids were stained with fixable life/dead staining (LD), nuclei were counter stained with Hoechst. (Scale bar = 25 μm). G) Bar graph depicting relative caspase 3/7 activity measured by Caspase-Glo in ARID1A-expressing (PDT20, PDT36, PDT39) and ARID1A-deficient (PDT3, PDT30, PDT37) PDTs following treatment with 32nM prexasertib or 50nM YM155 for two days. (triplicates of two independent experiments; mean ± SEM), two-way ANOVA, ***p<0.0005, ****p<0.00005.) H) Fluorescence staining of caspase activity reporter on ARID1A-expressing (PDT36) and ARID1A-deficient (PDT30) PDTs treated with 32nM prexasertib or 50nM YM155 for two days, nuclei were counter stained with Hoechst. (Scale bar = 25 μm).

**Techniques Used: **Expressing, Control, Flow Cytometry, Concentration Assay, Fluorescence, Staining, Activity Assay

# annexin v propidium iodide pi kit (Becton Dickinson)

## Structured Review

Annexin V Propidium Iodide Pi Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/annexin v propidium iodide pi kit/product/Becton Dickinson

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

# propidium iodide pi (Becton Dickinson)

## Structured Review

Propidium Iodide Pi, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/propidium iodide pi/product/Becton Dickinson

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99

## Images

### 1) Product Images from "Cyclin A/B RxL Macrocyclic Inhibitors to Treat Cancers with High E2F Activity"

**Article Title: **Cyclin A/B RxL Macrocyclic Inhibitors to Treat Cancers with High E2F Activity

**Journal: **bioRxiv

**doi: **10.1101/2024.08.01.605889

**Figure Legend Snippet:**A) Structure of cyclin A (PDB code: 1JSU) with blue indicating basic, red acidic and white neutral surfaces. “HP” indicates the Hydrophobic Patch formed in part by the highly conserved MRAIL motif in cyclins and “S” indicates the smaller adjacent hydrophobic pocket. B) Docked model of CIRc-004 filled-in structure in green bound to cyclin A. Shown next to it is the detailed “stick” structure representation of CIRc-004 docked to cyclin A. C) Biochemical activity of cyclin RxL macrocyclic inhibitors in cyclin A1/Cdk2, cyclinE1/Cdk2 and cyclin B/CDK1 complexes measured by Fluorescence Polarization. D ) GI 50 waterfall plot of 46 human SCLC cell lines panel. E ) Heatmap displays the activity patterns of various Hallmark pathways within a panel of 46 SCLC cell lines. The Gene Set Variation Analysis (GSVA) method, utilizing the MSigDb Hallmark collection of RNA-seq data, was employed to calculate scores for each pathway. Each column represents a distinct SCLC cell line ordered from most sensitive to least sensitive based on GI 50 values for cyclin RxL A/B inhibitor (CIRc-004). p-value <0.05 are shown and calculated by Wilcoxon rank sum exact test. F ) Dose response assays of the indicated human SCLC cell lines (NCI-H1048, NCI-H446, and NCI-H69) and human NSCLC cell lines (A549, HCC4006, and NCI-H1299) treated for 6 days with increasing doses of the cyclin A/B RxL inhibitor (CIRc-004). Average half-maximal effective concentration (EC50) for cyclin A/B RxL inhibitor (CIRc-004) shown next to respective cell line. G ) Representative flow cytometry analysis of cleaved PARP in NCI-H1048 cells treated with CIRc-004 (200 nM) or DMSO (vehicle) for 3 days. H ) Quantitation of cleaved PARP positive cells analyzed by flow cytometric analysis in the different SCLC and NSCLC lung cancer cell lines treated for 3 days with the indicated doses of the cyclin A/B RxL inhibitor (CIRc-004). I ) Cell cycle distribution of the indicated SCLC and NSCLC cell lines treated with CIRc-004 (200 nM) or DMSO (vehicle) for 24 hours and then stained with propidium iodide (PI). For F, H and I , n=3 biological independent experiments and data are mean +/− SD. Arrow in F indicates DMSO-treated sample which was used for normalization. Statistical significance for H was calculated using unpaired, two-tailed students t -test. Where indicated, *=p<0,05, **=p<0.01, ***=p<0.001, ****=p<0.000.

**Techniques Used: **Activity Assay, Fluorescence, RNA Sequencing Assay, Concentration Assay, Flow Cytometry, Quantitation Assay, Staining, Two Tailed Test

# 40x propidium iodide pi (Millipore)

## Structured Review

40x Propidium Iodide Pi, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

https://www.bioz.com/result/40x propidium iodide pi/product/Millipore

Average 86 stars, based on 1 article reviews

Price from $9.99 to $1999.99