propac pa 1 analytical column  (Thermo Fisher)


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    Name:
    ProPac PA1 LC Columns
    Description:
    Efficiently analyze and purify hydrophilic anionic proteins and peptides with Thermo Scientific ProPac PA1 LC Columns These strong anion exchange columns provide high resolution separations of proteins with pI values from 3 to 11
    Catalog Number:
    039657
    Price:
    None
    Category:
    Chromatography Columns Resins Spin Filters
    Applications:
    Industrial & Applied Science|Industrial Chromatography
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    Structured Review

    Thermo Fisher propac pa 1 analytical column
    Efficiently analyze and purify hydrophilic anionic proteins and peptides with Thermo Scientific ProPac PA1 LC Columns These strong anion exchange columns provide high resolution separations of proteins with pI values from 3 to 11
    https://www.bioz.com/result/propac pa 1 analytical column/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    propac pa 1 analytical column - by Bioz Stars, 2021-06
    93/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Comparative Assessment of the Effects of Gender-specific Heparan Sulfates on Mesenchymal Stem Cells *
    Article Snippet: .. The lyase-derived disaccharides were resolved by SAX-HPLC on a ProPac PA1 analytical column (4 × 250 mm) linked to a Dionex ICS-3000 HPLC system. .. After equilibration in the mobile phase (double-distilled water adjusted to pH 3.5 with HCl) at 1 ml/min, samples were injected, and disaccharides eluted with a linear gradient of sodium chloride from 0 to 1 m over 60 min in the same mobile phase.

    Article Title: Evolutionary Differences in Glycosaminoglycan Fine Structure Detected by Quantitative Glycan Reductive Isotope Labeling *Evolutionary Differences in Glycosaminoglycan Fine Structure Detected by Quantitative Glycan Reductive Isotope Labeling * S⃞
    Article Snippet: .. Heparin (Sigma) disaccharides were also separated by high-performance liquid chromatography on a ProPac PA1 anion-exchange column (Dionex) with a linear gradient of sodium chloride (50 m m to 1 m , pH 3.5, 60 min). ..

    Article Title: A mutant cell library for systematic analysis of heparan sulfate structure-function relationships
    Article Snippet: After inactivation at 95°C for 10 min, the released glycosaminoglycan was enriched by passing through a Q-column, desalting and then digested with heparin lyases I, II, and III (Sigma-Aldrich) overnight at 37 °C. .. The resultant HS disaccharides were collected by filtering the digested glycosaminoglycan though a 3000 MW cut-off filter (Thermo-Fisher), labeled with 2-aminobenzide and then subjected to separation by an Agilent 1260 HPLC system with a Propac PA1 (4 × 250 mm, DIONEX) column connected with a Propac PA1 Guard column (4 × 50 mm, DIONEX). .. The separated disaccharides were detected using a fluorescence detector with excitation at 348 nm and emission at 440 nm.

    Article Title: Melatonin protects lung mitochondria from aging
    Article Snippet: .. Adenine nucleotides were determined by HPLC with a ProPac PA1 column (4 × 250 mm; Dionex, Bannockburn, IL, USA) and a binary gradient of 0.3 M ammonium carbonate and water (Pissarek et al. ). .. Purified mitochondria were rapidly resuspended in ice-cold 0.5 M perchloric acid, mixed during 120 s in vortex (to break the mitochondrial membranes) and centrifuged at 25,000 × g for 15 min at 2°C to precipitate proteins.

    Article Title: Endothelial Heparan Sulfate 6-O-Sulfation Levels Regulate Angiogenic Responses of Endothelial Cells to Fibroblast Growth Factor 2 and Vascular Endothelial Growth Factor *
    Article Snippet: Residual chondroitin/dermatan sulfate was removed by digestion with 50 mIU chondroitinase ABC followed by DEAE-Sephacel chromatography. .. 3 H-Labeled HS was digested to disaccharides using a mixture of 2 mIU of each heparinase I, II, and III (Grampian Enzymes), and disaccharides were applied to a Propac PA1 SAX-HPLC column (4.6 × 250 mm; Dionex) and run on an Agilent 1200 Series HPLC system. .. Disaccharides were eluted using 45 ml of linear gradients of 0–1 m NaCl (pH 3.5) at a flow rate of 1 ml/min.

    Fluorescence:

    Article Title: Sulf1 and Sulf2 Differentially Modulate Heparan Sulfate Proteoglycan Sulfation during Postnatal Cerebellum Development: Evidence for Neuroprotective and Neurite Outgrowth Promoting Functions
    Article Snippet: .. HS disaccharides were labelled with Bodipy™ FL hydrazide (Invitrogen), separated by SAX-HPLC on a Propac PA-1 column (Dionex, UK), and detected in-line using a Shimadzu RF-551 fluorescence detector, as previously described [ , , ]. .. Disaccharide peaks were identified by reference to the consistent elution positions of authentic disaccharide standards, and peak quantification performed using Shimadzu software with baseline subtraction, using previously calculated correction factors to account for differential labeling [ ].

    Labeling:

    Article Title: A mutant cell library for systematic analysis of heparan sulfate structure-function relationships
    Article Snippet: After inactivation at 95°C for 10 min, the released glycosaminoglycan was enriched by passing through a Q-column, desalting and then digested with heparin lyases I, II, and III (Sigma-Aldrich) overnight at 37 °C. .. The resultant HS disaccharides were collected by filtering the digested glycosaminoglycan though a 3000 MW cut-off filter (Thermo-Fisher), labeled with 2-aminobenzide and then subjected to separation by an Agilent 1260 HPLC system with a Propac PA1 (4 × 250 mm, DIONEX) column connected with a Propac PA1 Guard column (4 × 50 mm, DIONEX). .. The separated disaccharides were detected using a fluorescence detector with excitation at 348 nm and emission at 440 nm.

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  • 93
    Thermo Fisher propac pa 1 analytical column
    Propac Pa 1 Analytical Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/propac pa 1 analytical column/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    propac pa 1 analytical column - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

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