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Promega promega purification kit
Promega Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/promega purification kit/product/Promega
Average 96 stars, based on 3 article reviews
Price from $9.99 to $1999.99
promega purification kit - by Bioz Stars, 2020-04
96/100 stars

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Clone Assay:

Article Title: Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Article Snippet: Captured fragments were amplified with AP11 primers and PCR products were cleaned up using the Promega Purification Kit. .. The transformed cells were cultured in LB agar with 70 μg/ml X-gal, 80 μM IPTG, 50 μg/ml Carbenicyllin, and 15 μg/ml Tetracyclin for 15–18 h, after which white clones were picked and cultured in LB broth with 50 μg/ml Carbenicyllin and 15 μg/ml Tetracyclin.

Article Title: Diversity and Inheritance of Intergenic Spacer Sequences of 45S Ribosomal DNA among Accessions of Brassica oleracea L. var. capitata
Article Snippet: Paragraph title: 4.3. PCR Amplification Cloning and Sequencing ... The amplified DNA fragments were purified using Promega Purification kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions.

Amplification:

Article Title: Identification and molecular characterization of Corynebacterium xerosis isolated from a sheep cutaneous abscess: first case report in Mexico
Article Snippet: As expected for the putative C. xerosis , a single PCR 446 bp amplicon band, corresponding to the rpoB gene fragment was amplified, and the intergenic 16S–23S gene and pld gene fragments were not amplified. .. RpoB gene amplicons of all the three samples were purified using the Promega purification kit (Wizard® SV Gel and PCR Clean-Up System) and were sent for automatic sequencing by Macrogen (Rockville, MD, USA).

Article Title: Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Article Snippet: .. Captured fragments were amplified with AP11 primers and PCR products were cleaned up using the Promega Purification Kit. .. The purified PCR products were ligated into pSTBlue-1 AccepTor Vector, and then transferred to NovaBlue SingleTM Competent Cells (Novagen, Merck Bioscience, Nottingham, GB).

Article Title: Diversity and Inheritance of Intergenic Spacer Sequences of 45S Ribosomal DNA among Accessions of Brassica oleracea L. var. capitata
Article Snippet: .. The amplified DNA fragments were purified using Promega Purification kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions. .. Cloning was performed using the TOPO TA cloning kit (Invitrogen, Carlsbad, CA, USA).

TA Cloning:

Article Title: Diversity and Inheritance of Intergenic Spacer Sequences of 45S Ribosomal DNA among Accessions of Brassica oleracea L. var. capitata
Article Snippet: The amplified DNA fragments were purified using Promega Purification kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions. .. Cloning was performed using the TOPO TA cloning kit (Invitrogen, Carlsbad, CA, USA).

Real-time Polymerase Chain Reaction:

Article Title: Immiscible Phase Nucleic Acid Purification Eliminates PCR Inhibitors with a Single Pass of Paramagnetic Particles through a Hydrophobic Liquid
Article Snippet: To demonstrate that IPF can process such challenging samples, we developed a qPCR assay to detect proviral HIV-1 DNA integrated into peripheral blood mononuclear cells. .. The Bland–Altman plot of the proviral DNA PCR assays shows the differences between the standard method using the Promega purification kit and the IPF method .

Incubation:

Article Title: Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Article Snippet: Captured fragments were amplified with AP11 primers and PCR products were cleaned up using the Promega Purification Kit. .. Fifty microliter bacteria liquid was incubated at 99°C for 5 min to lyse the bacteria and destroy the DNase.

Article Title: Diversity and Inheritance of Intergenic Spacer Sequences of 45S Ribosomal DNA among Accessions of Brassica oleracea L. var. capitata
Article Snippet: PCR amplifications were carried out by incubation at 95 °C for 3 min followed by 35 cycles of denaturation, annealing and extension. .. The amplified DNA fragments were purified using Promega Purification kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions.

Formalin-fixed Paraffin-Embedded:

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: However, the DNA quality was high enough for NGS when DNA was extracted using the other magnetic resin-based DNA extraction kit (Agencourt FormaPure DNA kit), although this kit showed lower extraction efficiency than the Promega purification kit. .. In terms of whole exome sequences from FFPE tissues, DNA extracted using the QIAamp DNA FFPE tissue kit and GeneRead DNA FFPE kit (both are membrane-based extraction kits from Qiagen) previously demonstrated higher DNA quality (in terms of DNA integrity), a higher variant calling frequency, and higher coverage of genes of interest than those extracted using the Promega purification kit [ ].

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: .. In terms of whole exome sequences from FFPE tissues, DNA extracted using the QIAamp DNA FFPE tissue kit and GeneRead DNA FFPE kit (both are membrane-based extraction kits from Qiagen) previously demonstrated higher DNA quality (in terms of DNA integrity), a higher variant calling frequency, and higher coverage of genes of interest than those extracted using the Promega purification kit [ ]. .. It is possible that the differences in variant calling and gene coverage, attributed to the different extraction methods, were not detected in our study owing to the small number of target genes analyzed.

Western Blot:

Article Title: Immiscible Phase Nucleic Acid Purification Eliminates PCR Inhibitors with a Single Pass of Paramagnetic Particles through a Hydrophobic Liquid
Article Snippet: HIV-1 proviral DNA was effectively purified from a 25-μl WB sample using the IPF method with 1.11 mg of PMPs from the Promega Magnesil gDNA purification kit. .. The Bland–Altman plot of the proviral DNA PCR assays shows the differences between the standard method using the Promega purification kit and the IPF method .

Transformation Assay:

Article Title: Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Article Snippet: Captured fragments were amplified with AP11 primers and PCR products were cleaned up using the Promega Purification Kit. .. The transformed cells were cultured in LB agar with 70 μg/ml X-gal, 80 μM IPTG, 50 μg/ml Carbenicyllin, and 15 μg/ml Tetracyclin for 15–18 h, after which white clones were picked and cultured in LB broth with 50 μg/ml Carbenicyllin and 15 μg/ml Tetracyclin.

Cell Culture:

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: Discussion In the present study, we clearly demonstrated that DNA obtained from cultured cells and clinical LBC specimens fixed with CytoRich Red solution were suitable for cancer genome analysis using NGS, even after the samples were stored for up to 1 year. .. However, the DNA quality was high enough for NGS when DNA was extracted using the other magnetic resin-based DNA extraction kit (Agencourt FormaPure DNA kit), although this kit showed lower extraction efficiency than the Promega purification kit.

Article Title: Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Article Snippet: Captured fragments were amplified with AP11 primers and PCR products were cleaned up using the Promega Purification Kit. .. The transformed cells were cultured in LB agar with 70 μg/ml X-gal, 80 μM IPTG, 50 μg/ml Carbenicyllin, and 15 μg/ml Tetracyclin for 15–18 h, after which white clones were picked and cultured in LB broth with 50 μg/ml Carbenicyllin and 15 μg/ml Tetracyclin.

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: In terms of whole exome sequences from FFPE tissues, DNA extracted using the QIAamp DNA FFPE tissue kit and GeneRead DNA FFPE kit (both are membrane-based extraction kits from Qiagen) previously demonstrated higher DNA quality (in terms of DNA integrity), a higher variant calling frequency, and higher coverage of genes of interest than those extracted using the Promega purification kit [ ]. .. Alternatively, it is possible that these previous results were not comparable to ours because we used cultured cells fixed with an alcohol-based fixative, rather than FFPE.

Polymerase Chain Reaction:

Article Title: Identification and molecular characterization of Corynebacterium xerosis isolated from a sheep cutaneous abscess: first case report in Mexico
Article Snippet: .. RpoB gene amplicons of all the three samples were purified using the Promega purification kit (Wizard® SV Gel and PCR Clean-Up System) and were sent for automatic sequencing by Macrogen (Rockville, MD, USA). .. Multiple sequence alignments obtained from a BLAST (NCBI) were analysed along with our isolate sequences using Clustal W analysis from Mega 6.0.6 (Fig. ).

Article Title: Immiscible Phase Nucleic Acid Purification Eliminates PCR Inhibitors with a Single Pass of Paramagnetic Particles through a Hydrophobic Liquid
Article Snippet: .. The Bland–Altman plot of the proviral DNA PCR assays shows the differences between the standard method using the Promega purification kit and the IPF method . ..

Article Title: Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Article Snippet: .. Captured fragments were amplified with AP11 primers and PCR products were cleaned up using the Promega Purification Kit. .. The purified PCR products were ligated into pSTBlue-1 AccepTor Vector, and then transferred to NovaBlue SingleTM Competent Cells (Novagen, Merck Bioscience, Nottingham, GB).

Article Title: Diversity and Inheritance of Intergenic Spacer Sequences of 45S Ribosomal DNA among Accessions of Brassica oleracea L. var. capitata
Article Snippet: Paragraph title: 4.3. PCR Amplification Cloning and Sequencing ... The amplified DNA fragments were purified using Promega Purification kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions.

Cellular Antioxidant Activity Assay:

Article Title: Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Article Snippet: The products were hybridized with the biotinylated SSR oligos (GA)13, (CA)13, (CAT)8, (CCA)8, (CAA)8, and (GAA)8. .. Captured fragments were amplified with AP11 primers and PCR products were cleaned up using the Promega Purification Kit.

DNA Extraction:

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: .. However, the DNA quality was high enough for NGS when DNA was extracted using the other magnetic resin-based DNA extraction kit (Agencourt FormaPure DNA kit), although this kit showed lower extraction efficiency than the Promega purification kit. ..

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: However, the DNA quality was high enough for NGS when DNA was extracted using the other magnetic resin-based DNA extraction kit (Agencourt FormaPure DNA kit), although this kit showed lower extraction efficiency than the Promega purification kit. .. In terms of whole exome sequences from FFPE tissues, DNA extracted using the QIAamp DNA FFPE tissue kit and GeneRead DNA FFPE kit (both are membrane-based extraction kits from Qiagen) previously demonstrated higher DNA quality (in terms of DNA integrity), a higher variant calling frequency, and higher coverage of genes of interest than those extracted using the Promega purification kit [ ].

Magnetic Beads:

Article Title: Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Article Snippet: Hybridized fragments were captured by streptavidin-coated magnetic beads (Dynal) and residual biotinylated SSR oligos removed with Microcon YM-100 columns (Millipore). .. Captured fragments were amplified with AP11 primers and PCR products were cleaned up using the Promega Purification Kit.

Multiplex PCR:

Article Title: Identification and molecular characterization of Corynebacterium xerosis isolated from a sheep cutaneous abscess: first case report in Mexico
Article Snippet: A Multiplex-PCR technique for amplification of partial sequences of the pld , 16S–23S and rpoB genes was performed using the protocol published by Pacheco in 2007 [ ]. .. RpoB gene amplicons of all the three samples were purified using the Promega purification kit (Wizard® SV Gel and PCR Clean-Up System) and were sent for automatic sequencing by Macrogen (Rockville, MD, USA).

Purification:

Article Title: Identification and molecular characterization of Corynebacterium xerosis isolated from a sheep cutaneous abscess: first case report in Mexico
Article Snippet: .. RpoB gene amplicons of all the three samples were purified using the Promega purification kit (Wizard® SV Gel and PCR Clean-Up System) and were sent for automatic sequencing by Macrogen (Rockville, MD, USA). .. Multiple sequence alignments obtained from a BLAST (NCBI) were analysed along with our isolate sequences using Clustal W analysis from Mega 6.0.6 (Fig. ).

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: .. However, the DNA quality was high enough for NGS when DNA was extracted using the other magnetic resin-based DNA extraction kit (Agencourt FormaPure DNA kit), although this kit showed lower extraction efficiency than the Promega purification kit. ..

Article Title: Immiscible Phase Nucleic Acid Purification Eliminates PCR Inhibitors with a Single Pass of Paramagnetic Particles through a Hydrophobic Liquid
Article Snippet: .. The Bland–Altman plot of the proviral DNA PCR assays shows the differences between the standard method using the Promega purification kit and the IPF method . ..

Article Title: Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Article Snippet: .. Captured fragments were amplified with AP11 primers and PCR products were cleaned up using the Promega Purification Kit. .. The purified PCR products were ligated into pSTBlue-1 AccepTor Vector, and then transferred to NovaBlue SingleTM Competent Cells (Novagen, Merck Bioscience, Nottingham, GB).

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: .. In terms of whole exome sequences from FFPE tissues, DNA extracted using the QIAamp DNA FFPE tissue kit and GeneRead DNA FFPE kit (both are membrane-based extraction kits from Qiagen) previously demonstrated higher DNA quality (in terms of DNA integrity), a higher variant calling frequency, and higher coverage of genes of interest than those extracted using the Promega purification kit [ ]. .. It is possible that the differences in variant calling and gene coverage, attributed to the different extraction methods, were not detected in our study owing to the small number of target genes analyzed.

Article Title: Diversity and Inheritance of Intergenic Spacer Sequences of 45S Ribosomal DNA among Accessions of Brassica oleracea L. var. capitata
Article Snippet: .. The amplified DNA fragments were purified using Promega Purification kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions. .. Cloning was performed using the TOPO TA cloning kit (Invitrogen, Carlsbad, CA, USA).

Sequencing:

Article Title: Identification and molecular characterization of Corynebacterium xerosis isolated from a sheep cutaneous abscess: first case report in Mexico
Article Snippet: .. RpoB gene amplicons of all the three samples were purified using the Promega purification kit (Wizard® SV Gel and PCR Clean-Up System) and were sent for automatic sequencing by Macrogen (Rockville, MD, USA). .. Multiple sequence alignments obtained from a BLAST (NCBI) were analysed along with our isolate sequences using Clustal W analysis from Mega 6.0.6 (Fig. ).

Article Title: Diversity and Inheritance of Intergenic Spacer Sequences of 45S Ribosomal DNA among Accessions of Brassica oleracea L. var. capitata
Article Snippet: Paragraph title: 4.3. PCR Amplification Cloning and Sequencing ... The amplified DNA fragments were purified using Promega Purification kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions.

Chloramphenicol Acetyltransferase Assay:

Article Title: Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Article Snippet: The products were hybridized with the biotinylated SSR oligos (GA)13, (CA)13, (CAT)8, (CCA)8, (CAA)8, and (GAA)8. .. Captured fragments were amplified with AP11 primers and PCR products were cleaned up using the Promega Purification Kit.

Plasmid Preparation:

Article Title: Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Article Snippet: Captured fragments were amplified with AP11 primers and PCR products were cleaned up using the Promega Purification Kit. .. The purified PCR products were ligated into pSTBlue-1 AccepTor Vector, and then transferred to NovaBlue SingleTM Competent Cells (Novagen, Merck Bioscience, Nottingham, GB).

Multiplex Assay:

Article Title: Identification and molecular characterization of Corynebacterium xerosis isolated from a sheep cutaneous abscess: first case report in Mexico
Article Snippet: Reactions were carried out using a commercial multiplex PCR kit (QIAGEN Multiplex PCR) following the manufacturer’s specifications. .. RpoB gene amplicons of all the three samples were purified using the Promega purification kit (Wizard® SV Gel and PCR Clean-Up System) and were sent for automatic sequencing by Macrogen (Rockville, MD, USA).

Selection:

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: In terms of whole exome sequences from FFPE tissues, DNA extracted using the QIAamp DNA FFPE tissue kit and GeneRead DNA FFPE kit (both are membrane-based extraction kits from Qiagen) previously demonstrated higher DNA quality (in terms of DNA integrity), a higher variant calling frequency, and higher coverage of genes of interest than those extracted using the Promega purification kit [ ]. .. In terms of whole exome sequences from FFPE tissues, DNA extracted using the QIAamp DNA FFPE tissue kit and GeneRead DNA FFPE kit (both are membrane-based extraction kits from Qiagen) previously demonstrated higher DNA quality (in terms of DNA integrity), a higher variant calling frequency, and higher coverage of genes of interest than those extracted using the Promega purification kit [ ].

Next-Generation Sequencing:

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: .. However, the DNA quality was high enough for NGS when DNA was extracted using the other magnetic resin-based DNA extraction kit (Agencourt FormaPure DNA kit), although this kit showed lower extraction efficiency than the Promega purification kit. ..

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: However, the DNA quality was high enough for NGS when DNA was extracted using the other magnetic resin-based DNA extraction kit (Agencourt FormaPure DNA kit), although this kit showed lower extraction efficiency than the Promega purification kit. .. In terms of whole exome sequences from FFPE tissues, DNA extracted using the QIAamp DNA FFPE tissue kit and GeneRead DNA FFPE kit (both are membrane-based extraction kits from Qiagen) previously demonstrated higher DNA quality (in terms of DNA integrity), a higher variant calling frequency, and higher coverage of genes of interest than those extracted using the Promega purification kit [ ].

Concentration Assay:

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: A higher density of 1 × 105 cells/mL resulted in a reduced concentration of amplifiable DNA, indicating that the DNA extraction kits displayed certain use limitations in our experimental conditions. .. However, the DNA quality was high enough for NGS when DNA was extracted using the other magnetic resin-based DNA extraction kit (Agencourt FormaPure DNA kit), although this kit showed lower extraction efficiency than the Promega purification kit.

Lysis:

Article Title: Immiscible Phase Nucleic Acid Purification Eliminates PCR Inhibitors with a Single Pass of Paramagnetic Particles through a Hydrophobic Liquid
Article Snippet: We adapted the Promega Magnesil gDNA purification kit, which consists of 10 steps (lysis, seven washes, drying, and elution), for use with the IPF, which consists of three steps (lysis, PMP transport through liquid wax, and elution). .. The Bland–Altman plot of the proviral DNA PCR assays shows the differences between the standard method using the Promega purification kit and the IPF method .

Variant Assay:

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: However, the DNA quality was high enough for NGS when DNA was extracted using the other magnetic resin-based DNA extraction kit (Agencourt FormaPure DNA kit), although this kit showed lower extraction efficiency than the Promega purification kit. .. In terms of whole exome sequences from FFPE tissues, DNA extracted using the QIAamp DNA FFPE tissue kit and GeneRead DNA FFPE kit (both are membrane-based extraction kits from Qiagen) previously demonstrated higher DNA quality (in terms of DNA integrity), a higher variant calling frequency, and higher coverage of genes of interest than those extracted using the Promega purification kit [ ].

Article Title: Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis
Article Snippet: .. In terms of whole exome sequences from FFPE tissues, DNA extracted using the QIAamp DNA FFPE tissue kit and GeneRead DNA FFPE kit (both are membrane-based extraction kits from Qiagen) previously demonstrated higher DNA quality (in terms of DNA integrity), a higher variant calling frequency, and higher coverage of genes of interest than those extracted using the Promega purification kit [ ]. .. It is possible that the differences in variant calling and gene coverage, attributed to the different extraction methods, were not detected in our study owing to the small number of target genes analyzed.

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  • 86
    Promega wizard genomic dna purificationn kit
    Wizard Genomic Dna Purificationn Kit, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard genomic dna purificationn kit/product/Promega
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wizard genomic dna purificationn kit - by Bioz Stars, 2020-04
    86/100 stars
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    87
    Promega genomic dna purificaion kit
    Genomic Dna Purificaion Kit, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna purificaion kit/product/Promega
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    genomic dna purificaion kit - by Bioz Stars, 2020-04
    87/100 stars
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    95
    Promega wizard genomic dna purificatio n kit
    Wizard Genomic Dna Purificatio N Kit, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard genomic dna purificatio n kit/product/Promega
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wizard genomic dna purificatio n kit - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

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