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TaKaRa primestar takara dntp tofinal
Primestar Takara Dntp Tofinal, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primestar takara dntp tofinal/product/TaKaRa
Average 87 stars, based on 1 article reviews
Price from $9.99 to $1999.99
primestar takara dntp tofinal - by Bioz Stars, 2020-04
87/100 stars

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pcr
amplicons
primestar buffer
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Related Articles

Clone Assay:

Article Title: Pea3 transcription factor promotes neurite outgrowth
Article Snippet: Amplification products of NFM and NFL promoters (both spanning a region from -953 to +64 for hNF-L and from -654 to +10 for hNF-M ) were cloned into KpnI - HindIII sites of pGL2 - Basic vector (Promega). .. Amplicons from first step of PCR were run at 46°C for six cycles with 10× PrimeStar buffer (Takara) to final concentration 1×, PrimeStar DNA polymerase (Takara) to final concentration 1.25 U and PrimeStar (Takara) dNTP tofinal concentration 200 μM each to initiate annealing of overlapping mutant regions.

Amplification:

Article Title: Pea3 transcription factor promotes neurite outgrowth
Article Snippet: Amplification products of NFM and NFL promoters (both spanning a region from -953 to +64 for hNF-L and from -654 to +10 for hNF-M ) were cloned into KpnI - HindIII sites of pGL2 - Basic vector (Promega). .. Amplicons from first step of PCR were run at 46°C for six cycles with 10× PrimeStar buffer (Takara) to final concentration 1×, PrimeStar DNA polymerase (Takara) to final concentration 1.25 U and PrimeStar (Takara) dNTP tofinal concentration 200 μM each to initiate annealing of overlapping mutant regions.

Concentration Assay:

Article Title: Pea3 transcription factor promotes neurite outgrowth
Article Snippet: .. Amplicons from first step of PCR were run at 46°C for six cycles with 10× PrimeStar buffer (Takara) to final concentration 1×, PrimeStar DNA polymerase (Takara) to final concentration 1.25 U and PrimeStar (Takara) dNTP tofinal concentration 200 μM each to initiate annealing of overlapping mutant regions. .. To obtain the full length Pea3 gene coding sequence, second step of PCR was run at 56°C for 30 cycles with the previous reaction mix completed with ultimate forward and reverse primers and additional PrimeStar DNA polymerase and PrimeStar buffer.

Mutagenesis:

Article Title: Pea3 transcription factor promotes neurite outgrowth
Article Snippet: .. Amplicons from first step of PCR were run at 46°C for six cycles with 10× PrimeStar buffer (Takara) to final concentration 1×, PrimeStar DNA polymerase (Takara) to final concentration 1.25 U and PrimeStar (Takara) dNTP tofinal concentration 200 μM each to initiate annealing of overlapping mutant regions. .. To obtain the full length Pea3 gene coding sequence, second step of PCR was run at 56°C for 30 cycles with the previous reaction mix completed with ultimate forward and reverse primers and additional PrimeStar DNA polymerase and PrimeStar buffer.

Polymerase Chain Reaction:

Article Title: Pea3 transcription factor promotes neurite outgrowth
Article Snippet: .. Amplicons from first step of PCR were run at 46°C for six cycles with 10× PrimeStar buffer (Takara) to final concentration 1×, PrimeStar DNA polymerase (Takara) to final concentration 1.25 U and PrimeStar (Takara) dNTP tofinal concentration 200 μM each to initiate annealing of overlapping mutant regions. .. To obtain the full length Pea3 gene coding sequence, second step of PCR was run at 56°C for 30 cycles with the previous reaction mix completed with ultimate forward and reverse primers and additional PrimeStar DNA polymerase and PrimeStar buffer.

Sequencing:

Article Title: Pea3 transcription factor promotes neurite outgrowth
Article Snippet: In the first step of PCR, 100 ng pcDNA3-mPea3 was mixed with the 1.25 U Pfu DNA polymerase (Promega), Pfu buffer (Promega) to final concentration 1X, dNTP mix to final concentration 200 μM each, and forward and reverse primers (at varied concentrations) with mutant codons were paired with ultimate reverse and ultimate forward primers that constraints wild type mouse Pea3 coding sequence, respectively, run at the 56°C annealing temperature. .. Amplicons from first step of PCR were run at 46°C for six cycles with 10× PrimeStar buffer (Takara) to final concentration 1×, PrimeStar DNA polymerase (Takara) to final concentration 1.25 U and PrimeStar (Takara) dNTP tofinal concentration 200 μM each to initiate annealing of overlapping mutant regions.

Binding Assay:

Article Title: Pea3 transcription factor promotes neurite outgrowth
Article Snippet: Cloning of wild type NFL and NFM promoters were cloned and mutations of the putative Pea3 binding regions on these promoters were introduced by using primers in Table . .. Amplicons from first step of PCR were run at 46°C for six cycles with 10× PrimeStar buffer (Takara) to final concentration 1×, PrimeStar DNA polymerase (Takara) to final concentration 1.25 U and PrimeStar (Takara) dNTP tofinal concentration 200 μM each to initiate annealing of overlapping mutant regions.

Plasmid Preparation:

Article Title: Pea3 transcription factor promotes neurite outgrowth
Article Snippet: Amplification products of NFM and NFL promoters (both spanning a region from -953 to +64 for hNF-L and from -654 to +10 for hNF-M ) were cloned into KpnI - HindIII sites of pGL2 - Basic vector (Promega). .. Amplicons from first step of PCR were run at 46°C for six cycles with 10× PrimeStar buffer (Takara) to final concentration 1×, PrimeStar DNA polymerase (Takara) to final concentration 1.25 U and PrimeStar (Takara) dNTP tofinal concentration 200 μM each to initiate annealing of overlapping mutant regions.

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    TaKaRa primestar takara dntp tofinal
    Primestar Takara Dntp Tofinal, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primestar takara dntp tofinal/product/TaKaRa
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primestar takara dntp tofinal - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

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