primestar hs dna polymerase standard protocol  (TaKaRa)

 
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    Name:
    PrimeSTAR HS DNA Polymerase
    Description:
    PrimeSTAR HS DNA Polymerase is a novel high fidelity DNA polymerase that allows high efficiency amplification of large DNA products up to 8 5 kb for human genomic DNA up to 22 kb for lambda DNA Its excellent performance is achieved by superior proofreading ability due to a robust 3 → 5 exonuclease activity The antibody mediated hot start HS feature provides improved specificity thus reducing background and proving useful during highly demanding cloning and sequencing applications PrimeSTAR HS is available in flexible and convenient formats
    Catalog Number:
    r010b
    Price:
    None
    Size:
    1 000 Units
    Category:
    PrimeSTAR HS DNA Polymerase High fidelity PCR PCR
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    Structured Review

    TaKaRa primestar hs dna polymerase standard protocol
    PrimeSTAR HS DNA Polymerase is a novel high fidelity DNA polymerase that allows high efficiency amplification of large DNA products up to 8 5 kb for human genomic DNA up to 22 kb for lambda DNA Its excellent performance is achieved by superior proofreading ability due to a robust 3 → 5 exonuclease activity The antibody mediated hot start HS feature provides improved specificity thus reducing background and proving useful during highly demanding cloning and sequencing applications PrimeSTAR HS is available in flexible and convenient formats
    https://www.bioz.com/result/primestar hs dna polymerase standard protocol/product/TaKaRa
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    primestar hs dna polymerase standard protocol - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Cloning of the Lycopene β-cyclase Gene in Nicotiana tabacum and Its Overexpression Confers Salt and Drought Tolerance
    Article Snippet: .. Cloning of Ntβ-LCY1 and Vector Construction The coding sequence of Ntβ-LCY1 gene was amplified by PCR using high-fidelity DNA polymerase (PrimeSTAR® HS DNA Polymerase, Takara, Otsu, Japan) from CB-1 leaves using the primers of β-LCY1-F & β-LCY1-R, and then cloned into the T vector (Takara). .. Clones containing the Ntβ-LCY1 gene were further sequenced to confirm their sequences.

    Amplification:

    Article Title: Cloning of the Lycopene β-cyclase Gene in Nicotiana tabacum and Its Overexpression Confers Salt and Drought Tolerance
    Article Snippet: .. Cloning of Ntβ-LCY1 and Vector Construction The coding sequence of Ntβ-LCY1 gene was amplified by PCR using high-fidelity DNA polymerase (PrimeSTAR® HS DNA Polymerase, Takara, Otsu, Japan) from CB-1 leaves using the primers of β-LCY1-F & β-LCY1-R, and then cloned into the T vector (Takara). .. Clones containing the Ntβ-LCY1 gene were further sequenced to confirm their sequences.

    Article Title: Regulation of Srpr Expression by miR-330-5p Controls Proliferation of Mouse Epidermal Keratinocyte
    Article Snippet: .. Plasmid Construction The cDNAs of the full length 3’ UTR of Srpr was amplified from the dorsal skin cDNA of wild-type mice by PCR using PrimeSTAR DNA Polymerase (Takara). ..

    Knock-Out:

    Article Title: Genetic Knock-Down of Hdac3 Does Not Modify Disease-Related Phenotypes in a Mouse Model of Huntington's Disease
    Article Snippet: .. The 5′ homology arm (4.7 kb), the 3′ homology arm (4.9 kb) and the knock-out region (4.5 kb) were generated by PCR using high fidelity Prime Star HS DNA polymerase (Takara) and RP23-24H23 BAC clone as a template. .. Aside from the homologous arms, the final vector (HighQ1B-mHdac3) also contains loxP sequences flanking the knock-out region (4.5 kb), a neomycine resistance cassette (Neo, for positive selection of the ES cells), and a diphtheria toxin gene cassette (DTA, for negative selection of the ES cells).

    Mouse Assay:

    Article Title: Regulation of Srpr Expression by miR-330-5p Controls Proliferation of Mouse Epidermal Keratinocyte
    Article Snippet: .. Plasmid Construction The cDNAs of the full length 3’ UTR of Srpr was amplified from the dorsal skin cDNA of wild-type mice by PCR using PrimeSTAR DNA Polymerase (Takara). ..

    Sequencing:

    Article Title: Cloning of the Lycopene β-cyclase Gene in Nicotiana tabacum and Its Overexpression Confers Salt and Drought Tolerance
    Article Snippet: .. Cloning of Ntβ-LCY1 and Vector Construction The coding sequence of Ntβ-LCY1 gene was amplified by PCR using high-fidelity DNA polymerase (PrimeSTAR® HS DNA Polymerase, Takara, Otsu, Japan) from CB-1 leaves using the primers of β-LCY1-F & β-LCY1-R, and then cloned into the T vector (Takara). .. Clones containing the Ntβ-LCY1 gene were further sequenced to confirm their sequences.

    Article Title: Cell Wall Anchoring of the Campylobacter Antigens to Lactococcus lactis
    Article Snippet: .. Construction of the Vectors Carrying the Fusion of the Campylobacter Genes to the DNA Fragment Encoding the Cell Wall Anchor Region of L. lactis YndF The primers UspAx and UspBx were used to amplify the DNA region encoding the signal sequence of Usp45 (amino acids 1–30) from the chromosome of L. lactis IL1403 [PrimeStar HS DNA Polymerase (TaKaRa)]. ..

    Generated:

    Article Title: Genetic Knock-Down of Hdac3 Does Not Modify Disease-Related Phenotypes in a Mouse Model of Huntington's Disease
    Article Snippet: .. The 5′ homology arm (4.7 kb), the 3′ homology arm (4.9 kb) and the knock-out region (4.5 kb) were generated by PCR using high fidelity Prime Star HS DNA polymerase (Takara) and RP23-24H23 BAC clone as a template. .. Aside from the homologous arms, the final vector (HighQ1B-mHdac3) also contains loxP sequences flanking the knock-out region (4.5 kb), a neomycine resistance cassette (Neo, for positive selection of the ES cells), and a diphtheria toxin gene cassette (DTA, for negative selection of the ES cells).

    BAC Assay:

    Article Title: Genetic Knock-Down of Hdac3 Does Not Modify Disease-Related Phenotypes in a Mouse Model of Huntington's Disease
    Article Snippet: .. The 5′ homology arm (4.7 kb), the 3′ homology arm (4.9 kb) and the knock-out region (4.5 kb) were generated by PCR using high fidelity Prime Star HS DNA polymerase (Takara) and RP23-24H23 BAC clone as a template. .. Aside from the homologous arms, the final vector (HighQ1B-mHdac3) also contains loxP sequences flanking the knock-out region (4.5 kb), a neomycine resistance cassette (Neo, for positive selection of the ES cells), and a diphtheria toxin gene cassette (DTA, for negative selection of the ES cells).

    Polymerase Chain Reaction:

    Article Title: Cloning of the Lycopene β-cyclase Gene in Nicotiana tabacum and Its Overexpression Confers Salt and Drought Tolerance
    Article Snippet: .. Cloning of Ntβ-LCY1 and Vector Construction The coding sequence of Ntβ-LCY1 gene was amplified by PCR using high-fidelity DNA polymerase (PrimeSTAR® HS DNA Polymerase, Takara, Otsu, Japan) from CB-1 leaves using the primers of β-LCY1-F & β-LCY1-R, and then cloned into the T vector (Takara). .. Clones containing the Ntβ-LCY1 gene were further sequenced to confirm their sequences.

    Article Title: Regulation of Srpr Expression by miR-330-5p Controls Proliferation of Mouse Epidermal Keratinocyte
    Article Snippet: .. Plasmid Construction The cDNAs of the full length 3’ UTR of Srpr was amplified from the dorsal skin cDNA of wild-type mice by PCR using PrimeSTAR DNA Polymerase (Takara). ..

    Article Title: Mechanical properties of DNA-like polymers
    Article Snippet: .. For analogs 3 , 4 , 5 , 6 and 9 , PCR reactions (100 µl) included 20 ng template, 0.4 mM forward and reverse primers, PrimeSTAR GC buffer (Takara), 0.2 mM each dNTP (again with dTTP completely replaced with analog, except for variant 9 , which completely replaced dATP), 2 M betaine (Sigma-Aldrich) and 5 U PrimeSTAR HS DNA polymerase (Takara). .. For analogs 7 and 8 , PCR reactions (100 µl) included 20 ng template, 0.4 mM forward and reverse primers, Pwo PCR buffer (Roche), GC-rich solution (Roche), 0.2 mM each dNTP (dTTP completely replaced with analog), 2 M betaine (Sigma-Aldrich) and 5 U Pwo SuperYield DNA Polymerase (Roche).

    Article Title: Characterization of a single mutation in TraQ in a strain of Escherichia coli partially resistant to Qβ infection
    Article Snippet: .. PCR was performed using the resultant cDNA as the template, PrimeSTAR®; HS DNA polymerase (Takara Bio Inc., Shiga, Japan), and the primers pACYC_rev2 and traA_r2. .. To determine the 3′-terminal sequence of traA mRNA, universal miRNA cloning linker (5′-rAppCTGTAGGCACCATCAAT-NH2-3′; New England Biolabs) was ligated with the 3′-terminus of total RNA of Anc(C) using T4 RNA ligase 1 (New England Biolabs).

    Article Title: An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species
    Article Snippet: .. Standard PCR amplifications were performed with Prime-Star DNA polymerase (Takara, Beijing, China). ..

    Article Title: Genetic Knock-Down of Hdac3 Does Not Modify Disease-Related Phenotypes in a Mouse Model of Huntington's Disease
    Article Snippet: .. The 5′ homology arm (4.7 kb), the 3′ homology arm (4.9 kb) and the knock-out region (4.5 kb) were generated by PCR using high fidelity Prime Star HS DNA polymerase (Takara) and RP23-24H23 BAC clone as a template. .. Aside from the homologous arms, the final vector (HighQ1B-mHdac3) also contains loxP sequences flanking the knock-out region (4.5 kb), a neomycine resistance cassette (Neo, for positive selection of the ES cells), and a diphtheria toxin gene cassette (DTA, for negative selection of the ES cells).

    Article Title: Initiation, extension, and termination of RNA synthesis by a paramyxovirus polymerase
    Article Snippet: .. The 3XFLAG tag was added to the N-terminus of the NiV L protein by nested polymerase chain reaction (PCR) (two rounds of PCR with overlapping oligos containing the 3XFLAG tag; Eton Bioscience, San Diego, CA) with PrimeSTAR DNA polymerase (Takara Bio, Shiga, Japan). .. The 5′ SacI / HindIII region from the untagged NiV L was then replaced by the 3XFLAG-L PCR fragment.

    Variant Assay:

    Article Title: Mechanical properties of DNA-like polymers
    Article Snippet: .. For analogs 3 , 4 , 5 , 6 and 9 , PCR reactions (100 µl) included 20 ng template, 0.4 mM forward and reverse primers, PrimeSTAR GC buffer (Takara), 0.2 mM each dNTP (again with dTTP completely replaced with analog, except for variant 9 , which completely replaced dATP), 2 M betaine (Sigma-Aldrich) and 5 U PrimeSTAR HS DNA polymerase (Takara). .. For analogs 7 and 8 , PCR reactions (100 µl) included 20 ng template, 0.4 mM forward and reverse primers, Pwo PCR buffer (Roche), GC-rich solution (Roche), 0.2 mM each dNTP (dTTP completely replaced with analog), 2 M betaine (Sigma-Aldrich) and 5 U Pwo SuperYield DNA Polymerase (Roche).

    Nested PCR:

    Article Title: Initiation, extension, and termination of RNA synthesis by a paramyxovirus polymerase
    Article Snippet: .. The 3XFLAG tag was added to the N-terminus of the NiV L protein by nested polymerase chain reaction (PCR) (two rounds of PCR with overlapping oligos containing the 3XFLAG tag; Eton Bioscience, San Diego, CA) with PrimeSTAR DNA polymerase (Takara Bio, Shiga, Japan). .. The 5′ SacI / HindIII region from the untagged NiV L was then replaced by the 3XFLAG-L PCR fragment.

    Plasmid Preparation:

    Article Title: Cloning of the Lycopene β-cyclase Gene in Nicotiana tabacum and Its Overexpression Confers Salt and Drought Tolerance
    Article Snippet: .. Cloning of Ntβ-LCY1 and Vector Construction The coding sequence of Ntβ-LCY1 gene was amplified by PCR using high-fidelity DNA polymerase (PrimeSTAR® HS DNA Polymerase, Takara, Otsu, Japan) from CB-1 leaves using the primers of β-LCY1-F & β-LCY1-R, and then cloned into the T vector (Takara). .. Clones containing the Ntβ-LCY1 gene were further sequenced to confirm their sequences.

    Article Title: Regulation of Srpr Expression by miR-330-5p Controls Proliferation of Mouse Epidermal Keratinocyte
    Article Snippet: .. Plasmid Construction The cDNAs of the full length 3’ UTR of Srpr was amplified from the dorsal skin cDNA of wild-type mice by PCR using PrimeSTAR DNA Polymerase (Takara). ..

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  • News
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  • Contact
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  • 99
    TaKaRa primestar hs dna polymerase standard protocol
    Primestar Hs Dna Polymerase Standard Protocol, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primestar hs dna polymerase standard protocol/product/TaKaRa
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    primestar hs dna polymerase standard protocol - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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