primestar high sensitivity hs dna polymerase  (TaKaRa)

 
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    Name:
    PrimeSTAR HS DNA Polymerase
    Description:
    PrimeSTAR HS DNA Polymerase is a novel high fidelity DNA polymerase that allows high efficiency amplification of large DNA products up to 8 5 kb for human genomic DNA up to 22 kb for lambda DNA Its excellent performance is achieved by superior proofreading ability due to a robust 3 → 5 exonuclease activity The antibody mediated hot start HS feature provides improved specificity thus reducing background and proving useful during highly demanding cloning and sequencing applications PrimeSTAR HS is available in flexible and convenient formats
    Catalog Number:
    r010b
    Price:
    None
    Size:
    1 000 Units
    Category:
    PrimeSTAR HS DNA Polymerase High fidelity PCR PCR
    Buy from Supplier


    Structured Review

    TaKaRa primestar high sensitivity hs dna polymerase
    PrimeSTAR HS DNA Polymerase is a novel high fidelity DNA polymerase that allows high efficiency amplification of large DNA products up to 8 5 kb for human genomic DNA up to 22 kb for lambda DNA Its excellent performance is achieved by superior proofreading ability due to a robust 3 → 5 exonuclease activity The antibody mediated hot start HS feature provides improved specificity thus reducing background and proving useful during highly demanding cloning and sequencing applications PrimeSTAR HS is available in flexible and convenient formats
    https://www.bioz.com/result/primestar high sensitivity hs dna polymerase/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primestar high sensitivity hs dna polymerase - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Cloning of the Lycopene β-cyclase Gene in Nicotiana tabacum and Its Overexpression Confers Salt and Drought Tolerance
    Article Snippet: .. Cloning of Ntβ-LCY1 and Vector Construction The coding sequence of Ntβ-LCY1 gene was amplified by PCR using high-fidelity DNA polymerase (PrimeSTAR® HS DNA Polymerase, Takara, Otsu, Japan) from CB-1 leaves using the primers of β-LCY1-F & β-LCY1-R, and then cloned into the T vector (Takara). .. Clones containing the Ntβ-LCY1 gene were further sequenced to confirm their sequences.

    Amplification:

    Article Title: Cloning of the Lycopene β-cyclase Gene in Nicotiana tabacum and Its Overexpression Confers Salt and Drought Tolerance
    Article Snippet: .. Cloning of Ntβ-LCY1 and Vector Construction The coding sequence of Ntβ-LCY1 gene was amplified by PCR using high-fidelity DNA polymerase (PrimeSTAR® HS DNA Polymerase, Takara, Otsu, Japan) from CB-1 leaves using the primers of β-LCY1-F & β-LCY1-R, and then cloned into the T vector (Takara). .. Clones containing the Ntβ-LCY1 gene were further sequenced to confirm their sequences.

    Article Title: Regulation of the alternative splicing of tau exon 10 by SC35 and Dyrk1A
    Article Snippet: .. The crosslinking was reversed by incubation with 200 mM NaCl at 65°C for at least 2 h. After digestion with 0.4 mg/ml Proteinase K (Invitrogen) at 42°C for 45 min and 1 mg/ml of RQ1 Rnase-free Dnase (Promega) at 37°C for 15 min, respectively, RNA was extracted by RNeasy Mini Kit (Qiagen) and subjected to first-strand cDNA synthesis with random primer or Oligo-(dT)15–18 by using the Omniscript Reverse Transcription Kit (Qiagen). cDNA was amplified by PrimeSTARTM HS DNA Polymerase (Takara Bio Inc.) with two sets of primers against tau introns 9 and 10: one set primers: Forward 5′-AGGCGGGTCCAGGGTGGCGTGTCACTCATCC-3′, Reverse 5′-CTAATAATTCAAGCCACAGCACGGCGCATGGGACG-3′; another set of primers: Forward 5′-AGGGTGGCGCATGTCACTCATCGAAAGTGGAGGCG-3′, Reverse 5′-GGATTTATTCTATG CAGTGTCTCGCAAGTGTACGC-3′. .. PCR products were separated on a 1.5% agarose gel and visualized by ethidium bromide staining.

    Article Title: Solution structure of the Big domain from Streptococcus pneumoniae reveals a novel Ca2+-binding module
    Article Snippet: .. The plasmid pET22b(+)-SP0498 Big domain was used as a template, amplified by PCR using PrimeSTARTM HS DNA polymerase (TaKaRa, Dalian, China) and two complementary (partially overlapping) primers containing the desired mutation. .. The 50 μL PCR reaction was carried out with 50–100 ng templates, 10 μM primer pair, 200 μM dNTPs and 2 U of DNA polymerase, and started at 95°C for 3 min, followed by 20 cycles of 95°C for 45 s, 55°C for 45 s, and a final extension at 72°C for 8 min. After PCR reaction, the PCR product was digested with DpnI (TaKaRa, Dalian, China) overnight to remove methylated parental non-mutated plasmid, and then transformed into Escherichia coli BL21 (DE3).

    Mutagenesis:

    Article Title: Solution structure of the Big domain from Streptococcus pneumoniae reveals a novel Ca2+-binding module
    Article Snippet: .. The plasmid pET22b(+)-SP0498 Big domain was used as a template, amplified by PCR using PrimeSTARTM HS DNA polymerase (TaKaRa, Dalian, China) and two complementary (partially overlapping) primers containing the desired mutation. .. The 50 μL PCR reaction was carried out with 50–100 ng templates, 10 μM primer pair, 200 μM dNTPs and 2 U of DNA polymerase, and started at 95°C for 3 min, followed by 20 cycles of 95°C for 45 s, 55°C for 45 s, and a final extension at 72°C for 8 min. After PCR reaction, the PCR product was digested with DpnI (TaKaRa, Dalian, China) overnight to remove methylated parental non-mutated plasmid, and then transformed into Escherichia coli BL21 (DE3).

    Incubation:

    Article Title: Regulation of the alternative splicing of tau exon 10 by SC35 and Dyrk1A
    Article Snippet: .. The crosslinking was reversed by incubation with 200 mM NaCl at 65°C for at least 2 h. After digestion with 0.4 mg/ml Proteinase K (Invitrogen) at 42°C for 45 min and 1 mg/ml of RQ1 Rnase-free Dnase (Promega) at 37°C for 15 min, respectively, RNA was extracted by RNeasy Mini Kit (Qiagen) and subjected to first-strand cDNA synthesis with random primer or Oligo-(dT)15–18 by using the Omniscript Reverse Transcription Kit (Qiagen). cDNA was amplified by PrimeSTARTM HS DNA Polymerase (Takara Bio Inc.) with two sets of primers against tau introns 9 and 10: one set primers: Forward 5′-AGGCGGGTCCAGGGTGGCGTGTCACTCATCC-3′, Reverse 5′-CTAATAATTCAAGCCACAGCACGGCGCATGGGACG-3′; another set of primers: Forward 5′-AGGGTGGCGCATGTCACTCATCGAAAGTGGAGGCG-3′, Reverse 5′-GGATTTATTCTATG CAGTGTCTCGCAAGTGTACGC-3′. .. PCR products were separated on a 1.5% agarose gel and visualized by ethidium bromide staining.

    Knock-Out:

    Article Title: Genetic Knock-Down of Hdac3 Does Not Modify Disease-Related Phenotypes in a Mouse Model of Huntington's Disease
    Article Snippet: .. The 5′ homology arm (4.7 kb), the 3′ homology arm (4.9 kb) and the knock-out region (4.5 kb) were generated by PCR using high fidelity Prime Star HS DNA polymerase (Takara) and RP23-24H23 BAC clone as a template. .. Aside from the homologous arms, the final vector (HighQ1B-mHdac3) also contains loxP sequences flanking the knock-out region (4.5 kb), a neomycine resistance cassette (Neo, for positive selection of the ES cells), and a diphtheria toxin gene cassette (DTA, for negative selection of the ES cells).

    Sequencing:

    Article Title: Cloning of the Lycopene β-cyclase Gene in Nicotiana tabacum and Its Overexpression Confers Salt and Drought Tolerance
    Article Snippet: .. Cloning of Ntβ-LCY1 and Vector Construction The coding sequence of Ntβ-LCY1 gene was amplified by PCR using high-fidelity DNA polymerase (PrimeSTAR® HS DNA Polymerase, Takara, Otsu, Japan) from CB-1 leaves using the primers of β-LCY1-F & β-LCY1-R, and then cloned into the T vector (Takara). .. Clones containing the Ntβ-LCY1 gene were further sequenced to confirm their sequences.

    Article Title: Cell Wall Anchoring of the Campylobacter Antigens to Lactococcus lactis
    Article Snippet: .. Construction of the Vectors Carrying the Fusion of the Campylobacter Genes to the DNA Fragment Encoding the Cell Wall Anchor Region of L. lactis YndF The primers UspAx and UspBx were used to amplify the DNA region encoding the signal sequence of Usp45 (amino acids 1–30) from the chromosome of L. lactis IL1403 [PrimeStar HS DNA Polymerase (TaKaRa)]. ..

    Generated:

    Article Title: Genetic Knock-Down of Hdac3 Does Not Modify Disease-Related Phenotypes in a Mouse Model of Huntington's Disease
    Article Snippet: .. The 5′ homology arm (4.7 kb), the 3′ homology arm (4.9 kb) and the knock-out region (4.5 kb) were generated by PCR using high fidelity Prime Star HS DNA polymerase (Takara) and RP23-24H23 BAC clone as a template. .. Aside from the homologous arms, the final vector (HighQ1B-mHdac3) also contains loxP sequences flanking the knock-out region (4.5 kb), a neomycine resistance cassette (Neo, for positive selection of the ES cells), and a diphtheria toxin gene cassette (DTA, for negative selection of the ES cells).

    BAC Assay:

    Article Title: Genetic Knock-Down of Hdac3 Does Not Modify Disease-Related Phenotypes in a Mouse Model of Huntington's Disease
    Article Snippet: .. The 5′ homology arm (4.7 kb), the 3′ homology arm (4.9 kb) and the knock-out region (4.5 kb) were generated by PCR using high fidelity Prime Star HS DNA polymerase (Takara) and RP23-24H23 BAC clone as a template. .. Aside from the homologous arms, the final vector (HighQ1B-mHdac3) also contains loxP sequences flanking the knock-out region (4.5 kb), a neomycine resistance cassette (Neo, for positive selection of the ES cells), and a diphtheria toxin gene cassette (DTA, for negative selection of the ES cells).

    Polymerase Chain Reaction:

    Article Title: Cloning of the Lycopene β-cyclase Gene in Nicotiana tabacum and Its Overexpression Confers Salt and Drought Tolerance
    Article Snippet: .. Cloning of Ntβ-LCY1 and Vector Construction The coding sequence of Ntβ-LCY1 gene was amplified by PCR using high-fidelity DNA polymerase (PrimeSTAR® HS DNA Polymerase, Takara, Otsu, Japan) from CB-1 leaves using the primers of β-LCY1-F & β-LCY1-R, and then cloned into the T vector (Takara). .. Clones containing the Ntβ-LCY1 gene were further sequenced to confirm their sequences.

    Article Title: Mechanical properties of DNA-like polymers
    Article Snippet: .. For analogs 3 , 4 , 5 , 6 and 9 , PCR reactions (100 µl) included 20 ng template, 0.4 mM forward and reverse primers, PrimeSTAR GC buffer (Takara), 0.2 mM each dNTP (again with dTTP completely replaced with analog, except for variant 9 , which completely replaced dATP), 2 M betaine (Sigma-Aldrich) and 5 U PrimeSTAR HS DNA polymerase (Takara). .. For analogs 7 and 8 , PCR reactions (100 µl) included 20 ng template, 0.4 mM forward and reverse primers, Pwo PCR buffer (Roche), GC-rich solution (Roche), 0.2 mM each dNTP (dTTP completely replaced with analog), 2 M betaine (Sigma-Aldrich) and 5 U Pwo SuperYield DNA Polymerase (Roche).

    Article Title: Characterization of a single mutation in TraQ in a strain of Escherichia coli partially resistant to Qβ infection
    Article Snippet: .. PCR was performed using the resultant cDNA as the template, PrimeSTAR®; HS DNA polymerase (Takara Bio Inc., Shiga, Japan), and the primers pACYC_rev2 and traA_r2. .. To determine the 3′-terminal sequence of traA mRNA, universal miRNA cloning linker (5′-rAppCTGTAGGCACCATCAAT-NH2-3′; New England Biolabs) was ligated with the 3′-terminus of total RNA of Anc(C) using T4 RNA ligase 1 (New England Biolabs).

    Article Title: An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species
    Article Snippet: .. Standard PCR amplifications were performed with Prime-Star DNA polymerase (Takara, Beijing, China). ..

    Article Title: Solution structure of the Big domain from Streptococcus pneumoniae reveals a novel Ca2+-binding module
    Article Snippet: .. The plasmid pET22b(+)-SP0498 Big domain was used as a template, amplified by PCR using PrimeSTARTM HS DNA polymerase (TaKaRa, Dalian, China) and two complementary (partially overlapping) primers containing the desired mutation. .. The 50 μL PCR reaction was carried out with 50–100 ng templates, 10 μM primer pair, 200 μM dNTPs and 2 U of DNA polymerase, and started at 95°C for 3 min, followed by 20 cycles of 95°C for 45 s, 55°C for 45 s, and a final extension at 72°C for 8 min. After PCR reaction, the PCR product was digested with DpnI (TaKaRa, Dalian, China) overnight to remove methylated parental non-mutated plasmid, and then transformed into Escherichia coli BL21 (DE3).

    Article Title: Genetic Knock-Down of Hdac3 Does Not Modify Disease-Related Phenotypes in a Mouse Model of Huntington's Disease
    Article Snippet: .. The 5′ homology arm (4.7 kb), the 3′ homology arm (4.9 kb) and the knock-out region (4.5 kb) were generated by PCR using high fidelity Prime Star HS DNA polymerase (Takara) and RP23-24H23 BAC clone as a template. .. Aside from the homologous arms, the final vector (HighQ1B-mHdac3) also contains loxP sequences flanking the knock-out region (4.5 kb), a neomycine resistance cassette (Neo, for positive selection of the ES cells), and a diphtheria toxin gene cassette (DTA, for negative selection of the ES cells).

    Variant Assay:

    Article Title: Mechanical properties of DNA-like polymers
    Article Snippet: .. For analogs 3 , 4 , 5 , 6 and 9 , PCR reactions (100 µl) included 20 ng template, 0.4 mM forward and reverse primers, PrimeSTAR GC buffer (Takara), 0.2 mM each dNTP (again with dTTP completely replaced with analog, except for variant 9 , which completely replaced dATP), 2 M betaine (Sigma-Aldrich) and 5 U PrimeSTAR HS DNA polymerase (Takara). .. For analogs 7 and 8 , PCR reactions (100 µl) included 20 ng template, 0.4 mM forward and reverse primers, Pwo PCR buffer (Roche), GC-rich solution (Roche), 0.2 mM each dNTP (dTTP completely replaced with analog), 2 M betaine (Sigma-Aldrich) and 5 U Pwo SuperYield DNA Polymerase (Roche).

    Plasmid Preparation:

    Article Title: Cloning of the Lycopene β-cyclase Gene in Nicotiana tabacum and Its Overexpression Confers Salt and Drought Tolerance
    Article Snippet: .. Cloning of Ntβ-LCY1 and Vector Construction The coding sequence of Ntβ-LCY1 gene was amplified by PCR using high-fidelity DNA polymerase (PrimeSTAR® HS DNA Polymerase, Takara, Otsu, Japan) from CB-1 leaves using the primers of β-LCY1-F & β-LCY1-R, and then cloned into the T vector (Takara). .. Clones containing the Ntβ-LCY1 gene were further sequenced to confirm their sequences.

    Article Title: Solution structure of the Big domain from Streptococcus pneumoniae reveals a novel Ca2+-binding module
    Article Snippet: .. The plasmid pET22b(+)-SP0498 Big domain was used as a template, amplified by PCR using PrimeSTARTM HS DNA polymerase (TaKaRa, Dalian, China) and two complementary (partially overlapping) primers containing the desired mutation. .. The 50 μL PCR reaction was carried out with 50–100 ng templates, 10 μM primer pair, 200 μM dNTPs and 2 U of DNA polymerase, and started at 95°C for 3 min, followed by 20 cycles of 95°C for 45 s, 55°C for 45 s, and a final extension at 72°C for 8 min. After PCR reaction, the PCR product was digested with DpnI (TaKaRa, Dalian, China) overnight to remove methylated parental non-mutated plasmid, and then transformed into Escherichia coli BL21 (DE3).

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    TaKaRa primestar high sensitivity hs dna polymerase
    Primestar High Sensitivity Hs Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primestar high sensitivity hs dna polymerase/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primestar high sensitivity hs dna polymerase - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    90
    TaKaRa primerstar high sensitivity hs dna polymerase
    Primerstar High Sensitivity Hs Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primerstar high sensitivity hs dna polymerase/product/TaKaRa
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primerstar high sensitivity hs dna polymerase - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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