primescript rt master mix perfect real time kit  (TaKaRa)

 
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    Name:
    PrimeScript RT Master Mix
    Description:
    To minimize sample handling time look no further than this real time RT PCR kit PrimeScript RT Master Mix Perfect Real Time is designed for fast efficient reverse transcription prior to real time RT PCR qPCR with a streamlined workflow cDNA synthesis reactions are easy to assemble just add RNA template and water The real time RT PCR kit contains a 5X master mix reagent that includes all reagents required for cDNA synthesis PrimeScript RTase RNase inhibitor random 6mers oligo dT primer dNTPs and reaction buffer Because this real time RT PCR kit is powered by PrimeScript Reverse Transcriptase it enables full length cDNA synthesis for real time RT PCR qPCR in just 15 minutes This kit is designed for two step real time RT PCR Use it upstream of a TB Green or probe based qPCR premix from Takara Bio such as TB Green Premix Ex Taq II Tli RNase H TB Green Premix Ex Taq Tli RNase H or Premix Ex Taq Probe qPCR
    Catalog Number:
    rr036b
    Price:
    None
    Size:
    800 Rxns
    Category:
    Convenient master mix for real time PCR Reverse transcription prior to qPCR Real time PCR
    Buy from Supplier


    Structured Review

    TaKaRa primescript rt master mix perfect real time kit
    To minimize sample handling time look no further than this real time RT PCR kit PrimeScript RT Master Mix Perfect Real Time is designed for fast efficient reverse transcription prior to real time RT PCR qPCR with a streamlined workflow cDNA synthesis reactions are easy to assemble just add RNA template and water The real time RT PCR kit contains a 5X master mix reagent that includes all reagents required for cDNA synthesis PrimeScript RTase RNase inhibitor random 6mers oligo dT primer dNTPs and reaction buffer Because this real time RT PCR kit is powered by PrimeScript Reverse Transcriptase it enables full length cDNA synthesis for real time RT PCR qPCR in just 15 minutes This kit is designed for two step real time RT PCR Use it upstream of a TB Green or probe based qPCR premix from Takara Bio such as TB Green Premix Ex Taq II Tli RNase H TB Green Premix Ex Taq Tli RNase H or Premix Ex Taq Probe qPCR
    https://www.bioz.com/result/primescript rt master mix perfect real time kit/product/TaKaRa
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    primescript rt master mix perfect real time kit - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Delivery of inhibitor of growth 4 (ING4) gene significantly inhibits proliferation and invasion and promotes apoptosis of human osteosarcoma cells
    Article Snippet: .. Quantitative Real-time PCR (qRT-PCR) PrimeScript RT Master Mix and SYBR Premix Ex Taq II (Takara) were used for cDNA synthesis and SYBR Green qRT-PCR according to the manufacturer's protocols, respectively. .. The qRT-PCR assays were performed by Rotor gene Q (Qiagen).

    RNA Extraction:

    Article Title: Effect of overexpression of Oct4 and Sox2 genes on the biological and oncological characteristics of gastric cancer cells
    Article Snippet: .. Drugs and reagents We obtained Roswell Park Memorial Institute (RPMI) 1640 medium, TRizol total RNA extraction reagent, and diethyl pyrocarbonate (DEPC) water (TIANGEN BIOTECH, Beijing, China); Trans2K Plus II DNA Marker and FBS (Qiagen, Dusseldorf, Germany); goat anti-mouse secondary antibody, Oct4 antibody, Sox2 antibody, CD44 antibody, E-cadherin antibody, and β-actin antibody (Abcam, Cambridge, USA); PrimeScript RT Enzyme Mix I (x 200) and SYBR Green 820A (Takara, Tokyo, Japan); and Oct4 primer, Sox2 primer, CD44 primer, E-cadherin primer, KLF4 primer, c-Myc primer, B27 additive, human epidermal growth factor (EGF), and human basic fibroblast growth factor (bFGF; Invitrogen, Carlsbad, USA). ..

    Quantitative RT-PCR:

    Article Title: Delivery of inhibitor of growth 4 (ING4) gene significantly inhibits proliferation and invasion and promotes apoptosis of human osteosarcoma cells
    Article Snippet: .. Quantitative Real-time PCR (qRT-PCR) PrimeScript RT Master Mix and SYBR Premix Ex Taq II (Takara) were used for cDNA synthesis and SYBR Green qRT-PCR according to the manufacturer's protocols, respectively. .. The qRT-PCR assays were performed by Rotor gene Q (Qiagen).

    Article Title: MAZ mediates the cross-talk between CT-1 and NOTCH1 signaling during gliogenesis
    Article Snippet: .. The levels of ADAM10 mRNA were determined with qRT-PCR kit (Takara RR036A and RR820A). ..

    Article Title: Knockdown of SNHG16 suppresses the proliferation and induces the apoptosis of leukemia cells via miR-193a-5p/CDK8
    Article Snippet: .. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) The relative mRNA expression levels in the cells were determined by RT-qPCR. .. In brief, total RNA was extracted from the cells using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.).

    SYBR Green Assay:

    Article Title: Delivery of inhibitor of growth 4 (ING4) gene significantly inhibits proliferation and invasion and promotes apoptosis of human osteosarcoma cells
    Article Snippet: .. Quantitative Real-time PCR (qRT-PCR) PrimeScript RT Master Mix and SYBR Premix Ex Taq II (Takara) were used for cDNA synthesis and SYBR Green qRT-PCR according to the manufacturer's protocols, respectively. .. The qRT-PCR assays were performed by Rotor gene Q (Qiagen).

    Article Title: Effect of overexpression of Oct4 and Sox2 genes on the biological and oncological characteristics of gastric cancer cells
    Article Snippet: .. Drugs and reagents We obtained Roswell Park Memorial Institute (RPMI) 1640 medium, TRizol total RNA extraction reagent, and diethyl pyrocarbonate (DEPC) water (TIANGEN BIOTECH, Beijing, China); Trans2K Plus II DNA Marker and FBS (Qiagen, Dusseldorf, Germany); goat anti-mouse secondary antibody, Oct4 antibody, Sox2 antibody, CD44 antibody, E-cadherin antibody, and β-actin antibody (Abcam, Cambridge, USA); PrimeScript RT Enzyme Mix I (x 200) and SYBR Green 820A (Takara, Tokyo, Japan); and Oct4 primer, Sox2 primer, CD44 primer, E-cadherin primer, KLF4 primer, c-Myc primer, B27 additive, human epidermal growth factor (EGF), and human basic fibroblast growth factor (bFGF; Invitrogen, Carlsbad, USA). ..

    Marker:

    Article Title: Effect of overexpression of Oct4 and Sox2 genes on the biological and oncological characteristics of gastric cancer cells
    Article Snippet: .. Drugs and reagents We obtained Roswell Park Memorial Institute (RPMI) 1640 medium, TRizol total RNA extraction reagent, and diethyl pyrocarbonate (DEPC) water (TIANGEN BIOTECH, Beijing, China); Trans2K Plus II DNA Marker and FBS (Qiagen, Dusseldorf, Germany); goat anti-mouse secondary antibody, Oct4 antibody, Sox2 antibody, CD44 antibody, E-cadherin antibody, and β-actin antibody (Abcam, Cambridge, USA); PrimeScript RT Enzyme Mix I (x 200) and SYBR Green 820A (Takara, Tokyo, Japan); and Oct4 primer, Sox2 primer, CD44 primer, E-cadherin primer, KLF4 primer, c-Myc primer, B27 additive, human epidermal growth factor (EGF), and human basic fibroblast growth factor (bFGF; Invitrogen, Carlsbad, USA). ..

    other:

    Article Title: Inhibition of Calpains Protects Mn-Induced Neurotransmitter release disorders in Synaptosomes from Mice: Involvement of SNARE Complex and Synaptic Vesicle Fusion
    Article Snippet: PrimeScript® RT Enzyme Mix I and SYBR® Premix Ex TaqTM II kits were from TaKaRa Biotech.

    Expressing:

    Article Title: Knockdown of SNHG16 suppresses the proliferation and induces the apoptosis of leukemia cells via miR-193a-5p/CDK8
    Article Snippet: .. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) The relative mRNA expression levels in the cells were determined by RT-qPCR. .. In brief, total RNA was extracted from the cells using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.).

    Polymerase Chain Reaction:

    Article Title: Knockdown of SNHG16 suppresses the proliferation and induces the apoptosis of leukemia cells via miR-193a-5p/CDK8
    Article Snippet: .. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) The relative mRNA expression levels in the cells were determined by RT-qPCR. .. In brief, total RNA was extracted from the cells using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.).

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  • 99
    TaKaRa primescript rt master mix perfect real time kit
    Primescript Rt Master Mix Perfect Real Time Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primescript rt master mix perfect real time kit/product/TaKaRa
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    primescript rt master mix perfect real time kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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