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TaKaRa mrna expression
Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) measurements of gene expression in abomasal tissue of resistant and susceptible goats. ( a ), ( b ), ( c ), ( d ), ( e ) and ( f ): are the relative <t>mRNA</t> expression of Major histocompatibility complex class II molecules ( CIITA ), heat stress protein 70 ( HSPA8 ), signal transducer activator of transcription 5B ( STAT5B ), extended synaptotagmin-1 ( ESYT1 ), serpin peptidase inhibitor clade G member 1 ( SERPING1 ), and the endoplasmic Ca2+ pump ( <t>ATP2A3</t> ), respectively. Black colour bar represent the resistant group and ash colour bar represent the susceptible group of goats mRNA expression level. Error bars represent standard errors of the means. ** P
Mrna Expression, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 6984 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats"

Article Title: Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats

Journal: Journal of Animal Science and Biotechnology

doi: 10.1186/s40104-019-0327-8

Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) measurements of gene expression in abomasal tissue of resistant and susceptible goats. ( a ), ( b ), ( c ), ( d ), ( e ) and ( f ): are the relative mRNA expression of Major histocompatibility complex class II molecules ( CIITA ), heat stress protein 70 ( HSPA8 ), signal transducer activator of transcription 5B ( STAT5B ), extended synaptotagmin-1 ( ESYT1 ), serpin peptidase inhibitor clade G member 1 ( SERPING1 ), and the endoplasmic Ca2+ pump ( ATP2A3 ), respectively. Black colour bar represent the resistant group and ash colour bar represent the susceptible group of goats mRNA expression level. Error bars represent standard errors of the means. ** P
Figure Legend Snippet: Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) measurements of gene expression in abomasal tissue of resistant and susceptible goats. ( a ), ( b ), ( c ), ( d ), ( e ) and ( f ): are the relative mRNA expression of Major histocompatibility complex class II molecules ( CIITA ), heat stress protein 70 ( HSPA8 ), signal transducer activator of transcription 5B ( STAT5B ), extended synaptotagmin-1 ( ESYT1 ), serpin peptidase inhibitor clade G member 1 ( SERPING1 ), and the endoplasmic Ca2+ pump ( ATP2A3 ), respectively. Black colour bar represent the resistant group and ash colour bar represent the susceptible group of goats mRNA expression level. Error bars represent standard errors of the means. ** P

Techniques Used: Polymerase Chain Reaction, Quantitative RT-PCR, Expressing

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Amplification:

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Synthesized:

Article Title: Co-treatment with therapeutic neural stem cells expressing carboxyl esterase and CPT-11 inhibit growth of primary and metastatic lung cancers in mice
Article Snippet: .. Briefly, 1 μg of RNA from HB1.F3.CE and A549 lung cancer cells was reverse transcribed with murine leukemia virus reverse transcriptase (MMLV-RT; iNtRON Biotechnology, Sungnam, Kyeonggido, Korea), 10 pM dNTP (Bioneer, Deajeon, Korea), 200 pM monomer random primer (TaKaRa Bio., Shiga, Japan), 5 × RT buffer (iNtRON Biotechnology) and RNase inhibitor (iNtRON Biotechnology). cDNA was then synthesized at 37°C for one hour, after which the reaction was stopped by incubation at 95°C for 5 min. .. The cDNA produced from the extracted total RNA of HB1.F3.CE and A549 lung cancer cells was amplified by PCR to confirm the expression of human or rabbit CE genes.

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Northern Blot:

Article Title: Molecular Cloning and Functional Expression of the Potassium-Dependent Sodium–Calcium Exchanger from Human and Chicken Retinal Cone Photoreceptors
Article Snippet: .. Northern hybridization was performed using randomly labeled [α-32 P]dCTP-labeled cDNA probes (Prime-It II Random Primer Labeling Kit) in ExpressHyb hybridization solution (Clontech laboratories, Palo Alto, CA) at 68°C. .. A human normal brain mRNA blot loaded with mRNAs from the frontal lobe, temporal lobe, parietal lobe, occipital lobe, cerebellum, and lung (Northern Territory, Act-N, Invitrogen, Carlsbad, CA) was used according to the manufacturer's directions.

Incubation:

Article Title: Co-treatment with therapeutic neural stem cells expressing carboxyl esterase and CPT-11 inhibit growth of primary and metastatic lung cancers in mice
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Labeling:

Article Title: Molecular Cloning and Functional Expression of the Potassium-Dependent Sodium–Calcium Exchanger from Human and Chicken Retinal Cone Photoreceptors
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Article Title: Analysis of Expressed Sequence Tags and Characterization of a Novel Gene, Slmg7, in the Midgut of the Common Cutworm, Spodoptera litura
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Random Hexamer Labeling:

Article Title: Chemoresistance-related long non-coding RNA expression profiles in human breast cancer cells
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Cell Culture:

Article Title: Mammalian unfolded protein response inhibits cyclin D1 translation and cell-cycle progression
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Polymerase Chain Reaction:

Article Title: Two distinct aquaporin-4 cDNAs isolated from medullary cone of quail kidney ☆
Article Snippet: .. After synthesizing first-strand cDNA from the total RNA with a random hexamer primer, the expected AQP-related fragment was amplified with an AdvanTaq PCR Kit (Clontech Lab; Palo Alto, CA, USA) with a pair of degenerate primers selected from two conserved regions of the major intrinsic protein (MIP) family ( ; ). .. The fragment of the expected size (~ 370 bp) was subcloned into a PCR 2.1 T/A-vector (Invitrogen Life Tech.

Hybridization:

Article Title: Molecular Cloning and Functional Expression of the Potassium-Dependent Sodium–Calcium Exchanger from Human and Chicken Retinal Cone Photoreceptors
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