primer probe combinations  (Thermo Fisher)


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    Structured Review

    Thermo Fisher primer probe combinations
    Primer Probe Combinations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer probe combinations/product/Thermo Fisher
    Average 90 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    primer probe combinations - by Bioz Stars, 2020-07
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Reduced cholesterol and triglycerides in mice with a mutation in Mia2, a liver protein that localizes to ER exit sites [S]
    Article Snippet: .. Primer/probe combinations for quantitative reverse-transcriptase PCR (qRT-PCR) were purchased from Applied Biosystems (ABI), catalog number Mm00616368_m1 for mouse Mia2 and Mm00524692_m1 for mouse Ctage5 . qRT-PCR was done on an ABI 7900HT sequence detection system. ..

    Article Title: Analysis of Nkx3.1:Cre-driven Erk5 deletion reveals a profound spinal deformity which is linked to increased osteoclast activity
    Article Snippet: .. Specific primer/probe combinations (Table ) and Taqman Universal PCR Mastermix (Applied Biosystems) were added to cDNA samples (according to manufacturer’s instructions) before being run on an Applied Biosystems 7500 Fast Real-Time PCR System machine. ..

    Article Title: Mutant USA strain of porcine circovirus type 2 (mPCV2) exhibits similar virulence to the classical PCV2a and PCV2b strains in caesarean-derived, colostrum-deprived pigs
    Article Snippet: .. All serum samples, lung homogenates and superficial inguinal lymph node homogenates were tested for the presence of PCV2 DNA by qRT-PCR assays using primer/probe combinations as described ( ; ) with the following modifications: a commercially available master mix (TaqMan Universal PCR Master Mix; Applied Biosystems) was used, the reaction volume was 25 µl, only one replica was tested for each sample, and the thermal cycler conditions were 50 °C for 2 min, 95 °C for 10 min, followed by 40 cycles of 95 °C for 10 s and 60 °C for 1 min. ..

    Sequencing:

    Article Title: Reduced cholesterol and triglycerides in mice with a mutation in Mia2, a liver protein that localizes to ER exit sites [S]
    Article Snippet: .. Primer/probe combinations for quantitative reverse-transcriptase PCR (qRT-PCR) were purchased from Applied Biosystems (ABI), catalog number Mm00616368_m1 for mouse Mia2 and Mm00524692_m1 for mouse Ctage5 . qRT-PCR was done on an ABI 7900HT sequence detection system. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Unexpected patterns of Epstein–Barr virus transcription revealed by a High throughput PCR array for absolute quantification of viral mRNA
    Article Snippet: .. Design of TaqMan real-time PCR assays Forty five primer-probe combinations to detect selected EBV transcripts were designed using Primer Express (Life Technologies) under the default parameter settings. .. All primer-probe sequences (detailed in ) were based on the B95-8 reference genome ( ) and were chosen to avoid reported EBV sequence variations.

    Article Title: Analysis of Nkx3.1:Cre-driven Erk5 deletion reveals a profound spinal deformity which is linked to increased osteoclast activity
    Article Snippet: .. Specific primer/probe combinations (Table ) and Taqman Universal PCR Mastermix (Applied Biosystems) were added to cDNA samples (according to manufacturer’s instructions) before being run on an Applied Biosystems 7500 Fast Real-Time PCR System machine. ..

    Quantitative RT-PCR:

    Article Title: Reduced cholesterol and triglycerides in mice with a mutation in Mia2, a liver protein that localizes to ER exit sites [S]
    Article Snippet: .. Primer/probe combinations for quantitative reverse-transcriptase PCR (qRT-PCR) were purchased from Applied Biosystems (ABI), catalog number Mm00616368_m1 for mouse Mia2 and Mm00524692_m1 for mouse Ctage5 . qRT-PCR was done on an ABI 7900HT sequence detection system. ..

    Article Title: Mutant USA strain of porcine circovirus type 2 (mPCV2) exhibits similar virulence to the classical PCV2a and PCV2b strains in caesarean-derived, colostrum-deprived pigs
    Article Snippet: .. All serum samples, lung homogenates and superficial inguinal lymph node homogenates were tested for the presence of PCV2 DNA by qRT-PCR assays using primer/probe combinations as described ( ; ) with the following modifications: a commercially available master mix (TaqMan Universal PCR Master Mix; Applied Biosystems) was used, the reaction volume was 25 µl, only one replica was tested for each sample, and the thermal cycler conditions were 50 °C for 2 min, 95 °C for 10 min, followed by 40 cycles of 95 °C for 10 s and 60 °C for 1 min. ..

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  • 89
    Thermo Fisher primer probe combination
    Primer Probe Combination, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer probe combination/product/Thermo Fisher
    Average 89 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    primer probe combination - by Bioz Stars, 2020-07
    89/100 stars
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