primer moloney murine leukemia virus reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher primer moloney murine leukemia virus reverse transcriptase
    Primer Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer moloney murine leukemia virus reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primer moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Microarray analysis identifies a set of CXCR3 and CCR2 ligand chemokines as early IFN?-responsive genes in peripheral blood lymphocytes in vitro: an implication for IFN?-related adverse effects in multiple sclerosis
    Article Snippet: .. Quantitative real-time RT-PCR analysis DNase-treated total RNA was processed for cDNA synthesis using oligo(dT)12–18 primers and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA). cDNA was amplified by PCR in LightCycler ST300 (Roche Diagnostics, Tokyo, Japan) using SYBR Green I dye and the primer sets listed in Table . .. To calibrate the concentration of mRNA levels in test cDNA samples, serially-diluted purified PCR products generated by conventional RT-PCR (a 10-fold dilution from 1 pg/ml to 0.0001 pg/ml) were amplified in parallel.

    Amplification:

    Article Title: A survey of the sorghum transcriptome using single-molecule long reads
    Article Snippet: .. For APA validation, we used 3′ RACE (Invitrogen) strategy for cDNA synthesis and PCR amplification. cDNA was prepared using adaptor primer and PCR was carried out using an abridged universal (AUAP) reverse primer and gene-specific forward primer ( ). .. To confirm differential gene expression in response to PEG treatment, cDNA from control and treated tissues was used in qPCR with SYBR green master 1 mix (Roche) and gene-specific primers ( ).

    Article Title: Microarray analysis identifies a set of CXCR3 and CCR2 ligand chemokines as early IFN?-responsive genes in peripheral blood lymphocytes in vitro: an implication for IFN?-related adverse effects in multiple sclerosis
    Article Snippet: .. Quantitative real-time RT-PCR analysis DNase-treated total RNA was processed for cDNA synthesis using oligo(dT)12–18 primers and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA). cDNA was amplified by PCR in LightCycler ST300 (Roche Diagnostics, Tokyo, Japan) using SYBR Green I dye and the primer sets listed in Table . .. To calibrate the concentration of mRNA levels in test cDNA samples, serially-diluted purified PCR products generated by conventional RT-PCR (a 10-fold dilution from 1 pg/ml to 0.0001 pg/ml) were amplified in parallel.

    Article Title: BmDJ-1 Is a Key Regulator of Oxidative Modification in the Development of the Silkworm, Bombyx mori
    Article Snippet: .. DNase-treated total RNA was processed for cDNA synthesis using oligo(dT)12-18 primers and SuperScript II reverse transcriptase (Invitrogen), and cDNA was amplified by PCR using Pfu Turbo DNA polymerase (Stratagene, La Jolla, CA, USA) and the primers 5′-TCAAGAACAATGAGCAAGTCTGCG-3′ and 5′-TAATATTAGTACTGCGAGATTAAC-3′ . .. The amplified products were cloned into a cloning vector p3T (MoBiTec, Göttingen, Germany).

    Quantitative RT-PCR:

    Article Title: Microarray analysis identifies a set of CXCR3 and CCR2 ligand chemokines as early IFN?-responsive genes in peripheral blood lymphocytes in vitro: an implication for IFN?-related adverse effects in multiple sclerosis
    Article Snippet: .. Quantitative real-time RT-PCR analysis DNase-treated total RNA was processed for cDNA synthesis using oligo(dT)12–18 primers and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA). cDNA was amplified by PCR in LightCycler ST300 (Roche Diagnostics, Tokyo, Japan) using SYBR Green I dye and the primer sets listed in Table . .. To calibrate the concentration of mRNA levels in test cDNA samples, serially-diluted purified PCR products generated by conventional RT-PCR (a 10-fold dilution from 1 pg/ml to 0.0001 pg/ml) were amplified in parallel.

    Real-time Polymerase Chain Reaction:

    Article Title: Knockdown and replacement therapy mediated by artificial mirtrons in spinocerebellar ataxia 7
    Article Snippet: .. Small RNA qPCR assays for mirtrons were custom-designed by Life Technologies and carried out as above, except cDNA synthesis used gene-specific primers (supplied) with 100 ng total RNA, qPCR used TaqMan gene expression mastermix (Life Technologies) and values were normalized to GAPDH (Life Technologies). .. Natural miRNAs were assayed using Advanced miRNA Taqman assays (Thermo Scientific) for hsa-miR-16-1-3p (478727_miR), hsa-miR-21-3p (477973_miR), hsa-miR-124-3p (477879_miR) and hsa-miR-450b-5p (478914_miR), normalised to RNA18S5 (Hs03928985_g1).

    Random Hexamer Labeling:

    Article Title: Methods to create a stringent selection system for mammalian cell lines
    Article Snippet: .. Per sample 0.2 μg of RNA was treated with DNAseI followed by cDNA synthesis using random hexamer primers (SuperScript III, Invitrogen). .. The cDNA was diluted (1:100) followed by a real-time PCR (40 cycles in a two-step protocol) using the Cybr-Green Taq mix (Bio-Rad).

    Expressing:

    Article Title: Knockdown and replacement therapy mediated by artificial mirtrons in spinocerebellar ataxia 7
    Article Snippet: .. Small RNA qPCR assays for mirtrons were custom-designed by Life Technologies and carried out as above, except cDNA synthesis used gene-specific primers (supplied) with 100 ng total RNA, qPCR used TaqMan gene expression mastermix (Life Technologies) and values were normalized to GAPDH (Life Technologies). .. Natural miRNAs were assayed using Advanced miRNA Taqman assays (Thermo Scientific) for hsa-miR-16-1-3p (478727_miR), hsa-miR-21-3p (477973_miR), hsa-miR-124-3p (477879_miR) and hsa-miR-450b-5p (478914_miR), normalised to RNA18S5 (Hs03928985_g1).

    Polymerase Chain Reaction:

    Article Title: A survey of the sorghum transcriptome using single-molecule long reads
    Article Snippet: .. For APA validation, we used 3′ RACE (Invitrogen) strategy for cDNA synthesis and PCR amplification. cDNA was prepared using adaptor primer and PCR was carried out using an abridged universal (AUAP) reverse primer and gene-specific forward primer ( ). .. To confirm differential gene expression in response to PEG treatment, cDNA from control and treated tissues was used in qPCR with SYBR green master 1 mix (Roche) and gene-specific primers ( ).

    Article Title: Microarray analysis identifies a set of CXCR3 and CCR2 ligand chemokines as early IFN?-responsive genes in peripheral blood lymphocytes in vitro: an implication for IFN?-related adverse effects in multiple sclerosis
    Article Snippet: .. Quantitative real-time RT-PCR analysis DNase-treated total RNA was processed for cDNA synthesis using oligo(dT)12–18 primers and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA). cDNA was amplified by PCR in LightCycler ST300 (Roche Diagnostics, Tokyo, Japan) using SYBR Green I dye and the primer sets listed in Table . .. To calibrate the concentration of mRNA levels in test cDNA samples, serially-diluted purified PCR products generated by conventional RT-PCR (a 10-fold dilution from 1 pg/ml to 0.0001 pg/ml) were amplified in parallel.

    Article Title: BmDJ-1 Is a Key Regulator of Oxidative Modification in the Development of the Silkworm, Bombyx mori
    Article Snippet: .. DNase-treated total RNA was processed for cDNA synthesis using oligo(dT)12-18 primers and SuperScript II reverse transcriptase (Invitrogen), and cDNA was amplified by PCR using Pfu Turbo DNA polymerase (Stratagene, La Jolla, CA, USA) and the primers 5′-TCAAGAACAATGAGCAAGTCTGCG-3′ and 5′-TAATATTAGTACTGCGAGATTAAC-3′ . .. The amplified products were cloned into a cloning vector p3T (MoBiTec, Göttingen, Germany).

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  • 99
    Thermo Fisher moloney murine leukemia virus reverse transcriptase
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 682 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 682 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher moloney mouse leukaemia virus m mulv reverse transcriptase
    Moloney Mouse Leukaemia Virus M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney mouse leukaemia virus m mulv reverse transcriptase/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    moloney mouse leukaemia virus m mulv reverse transcriptase - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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    Thermo Fisher revertaidtm h m mulv first strand cdna synthesis kit
    Revertaidtm H M Mulv First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revertaidtm h m mulv first strand cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    revertaidtm h m mulv first strand cdna synthesis kit - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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