primer combinations  (Thermo Fisher)


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    Structured Review

    Thermo Fisher primer combinations
    Primer Combinations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer combinations/product/Thermo Fisher
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    primer combinations - by Bioz Stars, 2020-07
    91/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Selective interaction between nonribosomal peptide synthetases is facilitated by short communication-mediating domains
    Article Snippet: .. Gene fragments encoding TycA or C-terminal mutants thereof were amplified by using the primer combinations of 5′ tycA and different 3′ primers (see Table 2) and cloned into pTrcHis-TOPO expression vector (Invitrogen), providing an N-terminal hexahistidine tag. .. Similarly, the gene fragment encoding for TycB3 (domain organization A-PCP-E) was amplified by using oligonucleotides 5′ tycB3 and 3′ tycB3 and cloned into pTrcHis-TOPO.

    Article Title: The Arabidopsis NPF3 protein is a GA transporter
    Article Snippet: .. Cloning of NPF3 overexpression and reporter lines NPF3 was amplified with primer combinations listed in , cloned into pENTR/D-TOPO (Invitrogen K2400) and subsequently cloned into binary destination vectors using LR Gateway reaction (Invitrogen 11791). .. We have examined the phenotype and YFP fluorescence for 13 independent NPF3-YFP lines ( ).

    Amplification:

    Article Title: Selective interaction between nonribosomal peptide synthetases is facilitated by short communication-mediating domains
    Article Snippet: .. Gene fragments encoding TycA or C-terminal mutants thereof were amplified by using the primer combinations of 5′ tycA and different 3′ primers (see Table 2) and cloned into pTrcHis-TOPO expression vector (Invitrogen), providing an N-terminal hexahistidine tag. .. Similarly, the gene fragment encoding for TycB3 (domain organization A-PCP-E) was amplified by using oligonucleotides 5′ tycB3 and 3′ tycB3 and cloned into pTrcHis-TOPO.

    Article Title: Biased Gene Conversion in Rhizobium etli Is Caused by Preferential Double-Strand Breaks on One of the Recombining Homologs
    Article Snippet: .. For these primer combinations, the amplification protocol consisted of 30 cycles of denaturation (94°C, 40 s), annealing (44°C, 40 s), and extension (68°C, 40 s) using AccuPrime Taq DNA polymerase (Life Technologies). .. Both products were gel purified using a GeneJet extraction kit (Thermo Scientific).

    Article Title: Two Novel Genes Induced by Hard-Surface Contact of Colletotrichum gloeosporioides Conidia
    Article Snippet: .. A 532-bp segment amplified in the differential display with primer combinations of oligo(dT) primer HT11A and arbitrary 5′ decamer H-GP3 was subcloned into pCRII vector (Invitrogen, Carlsbad, Calif.) and designated CHIP2 (532). .. A 209-bp segment amplified in the differential display with primer combinations of oligo(dT) primer HT11A and arbitrary 5′ decamer H-AP1 was subcloned into pCRII vector and designated CHIP3 (209).

    Article Title: The Arabidopsis NPF3 protein is a GA transporter
    Article Snippet: .. Cloning of NPF3 overexpression and reporter lines NPF3 was amplified with primer combinations listed in , cloned into pENTR/D-TOPO (Invitrogen K2400) and subsequently cloned into binary destination vectors using LR Gateway reaction (Invitrogen 11791). .. We have examined the phenotype and YFP fluorescence for 13 independent NPF3-YFP lines ( ).

    Mutagenesis:

    Article Title: Evidence for dual targeting of Arabidopsis plastidial glucose-6-phosphate transporter GPT1 to peroxisomes via the ER
    Article Snippet: .. Site-directed Mutagenesis Single base changes, for destroying restriction sites or changing amino acids, were introduced by the Quick-Change PCR mutagenesis kit protocol (Stratagene), using the primer combinations listed in Supplemental Table 2 and PhusionTM High-Fidelity DNA Polymerase (Finnzymes). .. All base changes were confirmed by sequencing.

    Expressing:

    Article Title: Selective interaction between nonribosomal peptide synthetases is facilitated by short communication-mediating domains
    Article Snippet: .. Gene fragments encoding TycA or C-terminal mutants thereof were amplified by using the primer combinations of 5′ tycA and different 3′ primers (see Table 2) and cloned into pTrcHis-TOPO expression vector (Invitrogen), providing an N-terminal hexahistidine tag. .. Similarly, the gene fragment encoding for TycB3 (domain organization A-PCP-E) was amplified by using oligonucleotides 5′ tycB3 and 3′ tycB3 and cloned into pTrcHis-TOPO.

    Polymerase Chain Reaction:

    Article Title: Evidence for dual targeting of Arabidopsis plastidial glucose-6-phosphate transporter GPT1 to peroxisomes via the ER
    Article Snippet: .. Site-directed Mutagenesis Single base changes, for destroying restriction sites or changing amino acids, were introduced by the Quick-Change PCR mutagenesis kit protocol (Stratagene), using the primer combinations listed in Supplemental Table 2 and PhusionTM High-Fidelity DNA Polymerase (Finnzymes). .. All base changes were confirmed by sequencing.

    Over Expression:

    Article Title: The Arabidopsis NPF3 protein is a GA transporter
    Article Snippet: .. Cloning of NPF3 overexpression and reporter lines NPF3 was amplified with primer combinations listed in , cloned into pENTR/D-TOPO (Invitrogen K2400) and subsequently cloned into binary destination vectors using LR Gateway reaction (Invitrogen 11791). .. We have examined the phenotype and YFP fluorescence for 13 independent NPF3-YFP lines ( ).

    Plasmid Preparation:

    Article Title: Selective interaction between nonribosomal peptide synthetases is facilitated by short communication-mediating domains
    Article Snippet: .. Gene fragments encoding TycA or C-terminal mutants thereof were amplified by using the primer combinations of 5′ tycA and different 3′ primers (see Table 2) and cloned into pTrcHis-TOPO expression vector (Invitrogen), providing an N-terminal hexahistidine tag. .. Similarly, the gene fragment encoding for TycB3 (domain organization A-PCP-E) was amplified by using oligonucleotides 5′ tycB3 and 3′ tycB3 and cloned into pTrcHis-TOPO.

    Article Title: Two Novel Genes Induced by Hard-Surface Contact of Colletotrichum gloeosporioides Conidia
    Article Snippet: .. A 532-bp segment amplified in the differential display with primer combinations of oligo(dT) primer HT11A and arbitrary 5′ decamer H-GP3 was subcloned into pCRII vector (Invitrogen, Carlsbad, Calif.) and designated CHIP2 (532). .. A 209-bp segment amplified in the differential display with primer combinations of oligo(dT) primer HT11A and arbitrary 5′ decamer H-AP1 was subcloned into pCRII vector and designated CHIP3 (209).

    Software:

    Article Title: Mucin Muc2 Deficiency and Weaning Influences the Expression of the Innate Defense Genes Reg3?, Reg3? and Angiogenin-4
    Article Snippet: .. All primer combinations were designed using OLIGO 6.22 software (Molecular Biology Insights) and purchased from Invitrogen. ..

    Article Title: Rapid Diagnosis of Ebola Hemorrhagic Fever by Reverse Transcription-PCR in an Outbreak Setting and Assessment of Patient Viral Load as a Predictor of Outcome
    Article Snippet: .. The probe and primer combinations were designed by Primer Express software from Applied Biosystems. .. The reactions were thermocycled in an Applied Biosystems 7700 instrument by heating to 50°C for 2 min, followed by heating to 95°C for 10 min to activate the AmpliTaq polymerase.

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  • News
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  • 91
    Thermo Fisher primer combinations
    Primer Combinations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer combinations/product/Thermo Fisher
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    primer combinations - by Bioz Stars, 2020-07
    91/100 stars
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