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Seegene primer combinations
Primer Combinations, supplied by Seegene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primer combinations/product/Seegene
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
primer combinations - by Bioz Stars, 2020-07
91/100 stars

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Clone Assay:

Article Title: Gain and loss of polyadenylation signals during evolution of green algae
Article Snippet: .. Pyramimonas parkeae was cultured in modified ASP medium [ ]. mRNA was isolated from interphase cultures using the mRNA Isolation Kit (Roche Applied Science). mRNAs (50 ng) were converted into cDNA using the CapFishing™Full-length cDNA Premix Kit (Seegene) and then amplified by PCR with either of the following primer combinations: 5'-RACE (Seegene) and (5'-TTTTTTTTTTTTTTTTN3') (Coleochaete scutata ), or 3'-RACE (Seegene) and C1 (5'-NNNNNNNNNNNNNNNATG-3') (Klebsormidium subtile and Pyramimonas parkeae ). cDNA was size-fractioned by agarose gel electrophoresis and the smear between 500 bp-5000 bp was isolated and cloned into pGEM-T Easy vector (Promega). .. All libraries were transformed into ElectroMAX DH10B E. coli cells (Invitrogen) by electroporation.

Amplification:

Article Title: Gain and loss of polyadenylation signals during evolution of green algae
Article Snippet: .. Pyramimonas parkeae was cultured in modified ASP medium [ ]. mRNA was isolated from interphase cultures using the mRNA Isolation Kit (Roche Applied Science). mRNAs (50 ng) were converted into cDNA using the CapFishing™Full-length cDNA Premix Kit (Seegene) and then amplified by PCR with either of the following primer combinations: 5'-RACE (Seegene) and (5'-TTTTTTTTTTTTTTTTN3') (Coleochaete scutata ), or 3'-RACE (Seegene) and C1 (5'-NNNNNNNNNNNNNNNATG-3') (Klebsormidium subtile and Pyramimonas parkeae ). cDNA was size-fractioned by agarose gel electrophoresis and the smear between 500 bp-5000 bp was isolated and cloned into pGEM-T Easy vector (Promega). .. All libraries were transformed into ElectroMAX DH10B E. coli cells (Invitrogen) by electroporation.

Agarose Gel Electrophoresis:

Article Title: Gain and loss of polyadenylation signals during evolution of green algae
Article Snippet: .. Pyramimonas parkeae was cultured in modified ASP medium [ ]. mRNA was isolated from interphase cultures using the mRNA Isolation Kit (Roche Applied Science). mRNAs (50 ng) were converted into cDNA using the CapFishing™Full-length cDNA Premix Kit (Seegene) and then amplified by PCR with either of the following primer combinations: 5'-RACE (Seegene) and (5'-TTTTTTTTTTTTTTTTN3') (Coleochaete scutata ), or 3'-RACE (Seegene) and C1 (5'-NNNNNNNNNNNNNNNATG-3') (Klebsormidium subtile and Pyramimonas parkeae ). cDNA was size-fractioned by agarose gel electrophoresis and the smear between 500 bp-5000 bp was isolated and cloned into pGEM-T Easy vector (Promega). .. All libraries were transformed into ElectroMAX DH10B E. coli cells (Invitrogen) by electroporation.

Isolation:

Article Title: Gain and loss of polyadenylation signals during evolution of green algae
Article Snippet: .. Pyramimonas parkeae was cultured in modified ASP medium [ ]. mRNA was isolated from interphase cultures using the mRNA Isolation Kit (Roche Applied Science). mRNAs (50 ng) were converted into cDNA using the CapFishing™Full-length cDNA Premix Kit (Seegene) and then amplified by PCR with either of the following primer combinations: 5'-RACE (Seegene) and (5'-TTTTTTTTTTTTTTTTN3') (Coleochaete scutata ), or 3'-RACE (Seegene) and C1 (5'-NNNNNNNNNNNNNNNATG-3') (Klebsormidium subtile and Pyramimonas parkeae ). cDNA was size-fractioned by agarose gel electrophoresis and the smear between 500 bp-5000 bp was isolated and cloned into pGEM-T Easy vector (Promega). .. All libraries were transformed into ElectroMAX DH10B E. coli cells (Invitrogen) by electroporation.

Cell Culture:

Article Title: Gain and loss of polyadenylation signals during evolution of green algae
Article Snippet: .. Pyramimonas parkeae was cultured in modified ASP medium [ ]. mRNA was isolated from interphase cultures using the mRNA Isolation Kit (Roche Applied Science). mRNAs (50 ng) were converted into cDNA using the CapFishing™Full-length cDNA Premix Kit (Seegene) and then amplified by PCR with either of the following primer combinations: 5'-RACE (Seegene) and (5'-TTTTTTTTTTTTTTTTN3') (Coleochaete scutata ), or 3'-RACE (Seegene) and C1 (5'-NNNNNNNNNNNNNNNATG-3') (Klebsormidium subtile and Pyramimonas parkeae ). cDNA was size-fractioned by agarose gel electrophoresis and the smear between 500 bp-5000 bp was isolated and cloned into pGEM-T Easy vector (Promega). .. All libraries were transformed into ElectroMAX DH10B E. coli cells (Invitrogen) by electroporation.

Modification:

Article Title: Gain and loss of polyadenylation signals during evolution of green algae
Article Snippet: .. Pyramimonas parkeae was cultured in modified ASP medium [ ]. mRNA was isolated from interphase cultures using the mRNA Isolation Kit (Roche Applied Science). mRNAs (50 ng) were converted into cDNA using the CapFishing™Full-length cDNA Premix Kit (Seegene) and then amplified by PCR with either of the following primer combinations: 5'-RACE (Seegene) and (5'-TTTTTTTTTTTTTTTTN3') (Coleochaete scutata ), or 3'-RACE (Seegene) and C1 (5'-NNNNNNNNNNNNNNNATG-3') (Klebsormidium subtile and Pyramimonas parkeae ). cDNA was size-fractioned by agarose gel electrophoresis and the smear between 500 bp-5000 bp was isolated and cloned into pGEM-T Easy vector (Promega). .. All libraries were transformed into ElectroMAX DH10B E. coli cells (Invitrogen) by electroporation.

Polymerase Chain Reaction:

Article Title: Gain and loss of polyadenylation signals during evolution of green algae
Article Snippet: .. Pyramimonas parkeae was cultured in modified ASP medium [ ]. mRNA was isolated from interphase cultures using the mRNA Isolation Kit (Roche Applied Science). mRNAs (50 ng) were converted into cDNA using the CapFishing™Full-length cDNA Premix Kit (Seegene) and then amplified by PCR with either of the following primer combinations: 5'-RACE (Seegene) and (5'-TTTTTTTTTTTTTTTTN3') (Coleochaete scutata ), or 3'-RACE (Seegene) and C1 (5'-NNNNNNNNNNNNNNNATG-3') (Klebsormidium subtile and Pyramimonas parkeae ). cDNA was size-fractioned by agarose gel electrophoresis and the smear between 500 bp-5000 bp was isolated and cloned into pGEM-T Easy vector (Promega). .. All libraries were transformed into ElectroMAX DH10B E. coli cells (Invitrogen) by electroporation.

Plasmid Preparation:

Article Title: Gain and loss of polyadenylation signals during evolution of green algae
Article Snippet: .. Pyramimonas parkeae was cultured in modified ASP medium [ ]. mRNA was isolated from interphase cultures using the mRNA Isolation Kit (Roche Applied Science). mRNAs (50 ng) were converted into cDNA using the CapFishing™Full-length cDNA Premix Kit (Seegene) and then amplified by PCR with either of the following primer combinations: 5'-RACE (Seegene) and (5'-TTTTTTTTTTTTTTTTN3') (Coleochaete scutata ), or 3'-RACE (Seegene) and C1 (5'-NNNNNNNNNNNNNNNATG-3') (Klebsormidium subtile and Pyramimonas parkeae ). cDNA was size-fractioned by agarose gel electrophoresis and the smear between 500 bp-5000 bp was isolated and cloned into pGEM-T Easy vector (Promega). .. All libraries were transformed into ElectroMAX DH10B E. coli cells (Invitrogen) by electroporation.

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    Seegene primer combinations
    Primer Combinations, supplied by Seegene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer combinations/product/Seegene
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primer combinations - by Bioz Stars, 2020-07
    91/100 stars
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