primer combinations  (Millipore)


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    Structured Review

    Millipore primer combinations
    Primer Combinations, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer combinations/product/Millipore
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    primer combinations - by Bioz Stars, 2020-07
    91/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Isolation, characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)
    Article Snippet: .. Total of sixty recombinant clones i.e. thirty each from the two primer combinations were randomly picked and plasmid DNA from the transformed clones was isolated using plasmid elution kit (Sigma Aldrich) and confirmed by plasmid PCR. .. Plasmid DNAs were sequenced for further analysis.

    Article Title: A Novel Lactone-Forming Carboxylesterase: Molecular Identification of a Tuliposide A-Converting Enzyme in Tulip 1A Novel Lactone-Forming Carboxylesterase: Molecular Identification of a Tuliposide A-Converting Enzyme in Tulip 1 [W]
    Article Snippet: .. The second PCR yielded products of around 100 bp with all primer combinations, of which the products amplified by the primer combinations TCEA-N-F1/TCEA-Int-R1 for the first PCR and TCEA-N-F2/TCEA-Int-R2 for the second PCR were cloned into a pT7Blue T-vector (Novagen) and sequenced, resulting in the isolation of a 74-bp fragment. .. To isolate its 5′ and 3′ missing parts, 5′ and 3′ RACE-PCR was performed using the cDNAs synthesized above as templates.

    Article Title: Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion
    Article Snippet: .. For GST tagging of WarA and WarB the following primer combinations were used pWarAGST.1/pWarAGST.2 and pWarBGST.1/pWarBGST.2 and cloned into pET-41a-3CD, a vector derived from pET-41a by Novagen to produce fusion proteins with N-terminal GST-His-S tag. .. In all cases, transformed E. coli B834(DE3) cells were grown at 37 °C to an OD600 of about 0.6 in Tryptic Soy broth.

    Amplification:

    Article Title: A Novel Lactone-Forming Carboxylesterase: Molecular Identification of a Tuliposide A-Converting Enzyme in Tulip 1A Novel Lactone-Forming Carboxylesterase: Molecular Identification of a Tuliposide A-Converting Enzyme in Tulip 1 [W]
    Article Snippet: .. The second PCR yielded products of around 100 bp with all primer combinations, of which the products amplified by the primer combinations TCEA-N-F1/TCEA-Int-R1 for the first PCR and TCEA-N-F2/TCEA-Int-R2 for the second PCR were cloned into a pT7Blue T-vector (Novagen) and sequenced, resulting in the isolation of a 74-bp fragment. .. To isolate its 5′ and 3′ missing parts, 5′ and 3′ RACE-PCR was performed using the cDNAs synthesized above as templates.

    Mutagenesis:

    Article Title: Cohen Syndrome-associated Protein COH1 Physically and Functionally Interacts with the Small GTPase RAB6 at the Golgi Complex and Directs Neurite Outgrowth *
    Article Snippet: .. Using the resulting wild-type (wt) plasmids, T27N and Q72L mutants were obtained by overlap-PCR with primer combinations encoding the particular mutation and were subcloned into KpnI and NotI restriction sites of pFLAG-CMV6 (Sigma) or pFLAG-CMV5 (Sigma). .. For GFP-trap Co-IP, RAB6B wt and mutants were subcloned from pFLAG-CMV6 constructs into the restrictions sites HindIII and BamHI of pEGFPC1 (Clontech).

    Isolation:

    Article Title: Isolation, characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)
    Article Snippet: .. Total of sixty recombinant clones i.e. thirty each from the two primer combinations were randomly picked and plasmid DNA from the transformed clones was isolated using plasmid elution kit (Sigma Aldrich) and confirmed by plasmid PCR. .. Plasmid DNAs were sequenced for further analysis.

    Article Title: A Novel Lactone-Forming Carboxylesterase: Molecular Identification of a Tuliposide A-Converting Enzyme in Tulip 1A Novel Lactone-Forming Carboxylesterase: Molecular Identification of a Tuliposide A-Converting Enzyme in Tulip 1 [W]
    Article Snippet: .. The second PCR yielded products of around 100 bp with all primer combinations, of which the products amplified by the primer combinations TCEA-N-F1/TCEA-Int-R1 for the first PCR and TCEA-N-F2/TCEA-Int-R2 for the second PCR were cloned into a pT7Blue T-vector (Novagen) and sequenced, resulting in the isolation of a 74-bp fragment. .. To isolate its 5′ and 3′ missing parts, 5′ and 3′ RACE-PCR was performed using the cDNAs synthesized above as templates.

    Purification:

    Article Title: A generic, cost-effective, and scalable cell lineage analysis platform
    Article Snippet: .. Following dilution of the purified PCR from the first PCR (1:100), sample-specific barcoding was performed using standard PCR with forward and reverse primer combinations (Sigma). .. The indexes within the primer sequences and their dual combination annotated the original SC samples and produced a ready-to-run TruSeq HT NGS library using the standard Illumina sequences.

    Positron Emission Tomography:

    Article Title: Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion
    Article Snippet: .. For GST tagging of WarA and WarB the following primer combinations were used pWarAGST.1/pWarAGST.2 and pWarBGST.1/pWarBGST.2 and cloned into pET-41a-3CD, a vector derived from pET-41a by Novagen to produce fusion proteins with N-terminal GST-His-S tag. .. In all cases, transformed E. coli B834(DE3) cells were grown at 37 °C to an OD600 of about 0.6 in Tryptic Soy broth.

    Plasmid Preparation:

    Article Title: Isolation, characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)
    Article Snippet: .. Total of sixty recombinant clones i.e. thirty each from the two primer combinations were randomly picked and plasmid DNA from the transformed clones was isolated using plasmid elution kit (Sigma Aldrich) and confirmed by plasmid PCR. .. Plasmid DNAs were sequenced for further analysis.

    Article Title: Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion
    Article Snippet: .. For GST tagging of WarA and WarB the following primer combinations were used pWarAGST.1/pWarAGST.2 and pWarBGST.1/pWarBGST.2 and cloned into pET-41a-3CD, a vector derived from pET-41a by Novagen to produce fusion proteins with N-terminal GST-His-S tag. .. In all cases, transformed E. coli B834(DE3) cells were grown at 37 °C to an OD600 of about 0.6 in Tryptic Soy broth.

    Polymerase Chain Reaction:

    Article Title: Isolation, characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)
    Article Snippet: .. Total of sixty recombinant clones i.e. thirty each from the two primer combinations were randomly picked and plasmid DNA from the transformed clones was isolated using plasmid elution kit (Sigma Aldrich) and confirmed by plasmid PCR. .. Plasmid DNAs were sequenced for further analysis.

    Article Title: A generic, cost-effective, and scalable cell lineage analysis platform
    Article Snippet: .. Following dilution of the purified PCR from the first PCR (1:100), sample-specific barcoding was performed using standard PCR with forward and reverse primer combinations (Sigma). .. The indexes within the primer sequences and their dual combination annotated the original SC samples and produced a ready-to-run TruSeq HT NGS library using the standard Illumina sequences.

    Article Title: A Novel Lactone-Forming Carboxylesterase: Molecular Identification of a Tuliposide A-Converting Enzyme in Tulip 1A Novel Lactone-Forming Carboxylesterase: Molecular Identification of a Tuliposide A-Converting Enzyme in Tulip 1 [W]
    Article Snippet: .. The second PCR yielded products of around 100 bp with all primer combinations, of which the products amplified by the primer combinations TCEA-N-F1/TCEA-Int-R1 for the first PCR and TCEA-N-F2/TCEA-Int-R2 for the second PCR were cloned into a pT7Blue T-vector (Novagen) and sequenced, resulting in the isolation of a 74-bp fragment. .. To isolate its 5′ and 3′ missing parts, 5′ and 3′ RACE-PCR was performed using the cDNAs synthesized above as templates.

    Article Title: A Comparison of Sulfate and Selenium Accumulation in Relation to the Expression of Sulfate Transporter Genes in Astragalus Species 1 Species 1 [OA]
    Article Snippet: .. Subsequently, semiquantitative PCR was performed as a 15-μL reaction using 1 μL of each first-strand cDNA solution, specific primer combinations ( ) for the respective sulfate transporters, and REDTaq mix (Sigma-Aldrich). .. To equalize PCR conditions, the melting temperature of all primers was in the range of 61°C to 63°C and the amplicon size of the PCR fragments was between 470 and 520 bp ( ).

    Article Title: Influence of Sulfur Deficiency on the Expression of Specific Sulfate Transporters and the Distribution of Sulfur, Selenium, and Molybdenum in Wheat 1
    Article Snippet: .. Subsequently, semiquantitative PCR was performed as a 15- μ L reaction using 1 μ L of each first-strand cDNA solution, specific primer combinations for the respective sulfate transporters , and Red Taq mix (Sigma-Aldrich) containing loading dye. .. The PCR amplification for expression analysis was stopped during the linear amplification phase of the expressed analyzed sulfate transporter.

    Transformation Assay:

    Article Title: Isolation, characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)
    Article Snippet: .. Total of sixty recombinant clones i.e. thirty each from the two primer combinations were randomly picked and plasmid DNA from the transformed clones was isolated using plasmid elution kit (Sigma Aldrich) and confirmed by plasmid PCR. .. Plasmid DNAs were sequenced for further analysis.

    Recombinant:

    Article Title: Isolation, characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)
    Article Snippet: .. Total of sixty recombinant clones i.e. thirty each from the two primer combinations were randomly picked and plasmid DNA from the transformed clones was isolated using plasmid elution kit (Sigma Aldrich) and confirmed by plasmid PCR. .. Plasmid DNAs were sequenced for further analysis.

    Derivative Assay:

    Article Title: Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion
    Article Snippet: .. For GST tagging of WarA and WarB the following primer combinations were used pWarAGST.1/pWarAGST.2 and pWarBGST.1/pWarBGST.2 and cloned into pET-41a-3CD, a vector derived from pET-41a by Novagen to produce fusion proteins with N-terminal GST-His-S tag. .. In all cases, transformed E. coli B834(DE3) cells were grown at 37 °C to an OD600 of about 0.6 in Tryptic Soy broth.

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  • 85
    Millipore primer combinations a039 a064
    Primer Combinations A039 A064, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer combinations a039 a064/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primer combinations a039 a064 - by Bioz Stars, 2020-07
    85/100 stars
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