primer combination  (Thermo Fisher)


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    Structured Review

    Thermo Fisher primer combination
    Primer Combination, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer combination/product/Thermo Fisher
    Average 90 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    primer combination - by Bioz Stars, 2020-07
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: MHC class II DRB diversity, selection pattern and population structure in a neotropical bat species, Noctilio albiventris
    Article Snippet: .. At least 23 recombinant clones per primer combination were amplified using the vector primers T7for and M13rev, sequenced using BigDye Terminator v.3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and analysed on ABI PRISM 310 Automated Genetic Analyzer (Applied Biosystems). .. All primer combinations amplified the same two MHC class II DRB alleles in N. albiventris .

    Amplification:

    Article Title: Molecular evidence for hybridization in Colias (Lepidoptera: Pieridae): are Colias hybrids really hybrids?
    Article Snippet: .. AFLP amplicon data were collected on an ABI 3130XL Capillary Electrophoresis Genetic Analyzer (Applied Biosystems, Foster City, CA, United States) and compiled for each primer combination with ABI GeneMapper software version 3.7 (Life Technologies, Carlsbad, CA, United States). ..

    Article Title: Foot-and-Mouth Disease Virus-Associated Abortion and Vertical Transmission following Acute Infection in Cattle under Natural Conditions
    Article Snippet: .. For phylogenetic comparisons, PCR amplification of VP1 coding regions of FMDV were performed using the primer combination of ARS4 [ ] and NK61 in combination with Fermentas Pfu DNA polymerase (Thermo Fisher Scientific, Waltham, MA USA) as previously described [ ]. .. The PCR products were purified using QIA quick Gel Extraction Kit (QIAGEN, Hilden, Germany).

    Article Title: Molecular Approach to the Identification of Fish in the South China Sea
    Article Snippet: .. PCR amplifications were performed with the following conditions: 35 cycles of denaturation at 94°C for 45 s, annealing at 52–62°C (depending on the primer combination) for 50 s, and extension at 72°C for 1 min, with an initial denaturation at 94°C for 2 min and final extension at 72°C for 5 min. Amplified products were visualized in 1% agarose gel, and purified products were directly sequenced on an Applied Biosystems 3730 sequencer using the BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Biosystems, Inc.). ..

    Article Title: MHC class II DRB diversity, selection pattern and population structure in a neotropical bat species, Noctilio albiventris
    Article Snippet: .. At least 23 recombinant clones per primer combination were amplified using the vector primers T7for and M13rev, sequenced using BigDye Terminator v.3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and analysed on ABI PRISM 310 Automated Genetic Analyzer (Applied Biosystems). .. All primer combinations amplified the same two MHC class II DRB alleles in N. albiventris .

    Agarose Gel Electrophoresis:

    Article Title: Molecular Approach to the Identification of Fish in the South China Sea
    Article Snippet: .. PCR amplifications were performed with the following conditions: 35 cycles of denaturation at 94°C for 45 s, annealing at 52–62°C (depending on the primer combination) for 50 s, and extension at 72°C for 1 min, with an initial denaturation at 94°C for 2 min and final extension at 72°C for 5 min. Amplified products were visualized in 1% agarose gel, and purified products were directly sequenced on an Applied Biosystems 3730 sequencer using the BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Biosystems, Inc.). ..

    Purification:

    Article Title: Molecular Approach to the Identification of Fish in the South China Sea
    Article Snippet: .. PCR amplifications were performed with the following conditions: 35 cycles of denaturation at 94°C for 45 s, annealing at 52–62°C (depending on the primer combination) for 50 s, and extension at 72°C for 1 min, with an initial denaturation at 94°C for 2 min and final extension at 72°C for 5 min. Amplified products were visualized in 1% agarose gel, and purified products were directly sequenced on an Applied Biosystems 3730 sequencer using the BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Biosystems, Inc.). ..

    Article Title: Body Size Adaptations to Altitudinal Climatic Variation in Neotropical Grasshoppers of the Genus Sphenarium (Orthoptera: Pyrgomorphidae)
    Article Snippet: .. Long-PCR conditions included 2 min of initial denaturation at 92°C; 39 cycles of 92°C for 30 s, 50°C for 30 s, 60°C for 5 min; and a final extension at 60°C for 20 min. Other PCR reactions were performed using an initial denaturation at 92°C for 2 min, followed by 34 cycles of 92°C for 30 s, 30 s at the specific Tm for each primer combination (see ), 2 min at 60°C; and a final extension at 60°C for 10 min. Single-band PCR products were purified using PrepEase Purification 96-well plate kit (USB Corporation, Santa Clara, USA) and samples containing more than a single band were purified using QIAquick Gel Extraction Kit (Qiagen, Valencia, USA). .. We sequenced both strands of each purified products using BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) using Applied Biosystems 3730XL DNA Analyzer (Applied Biosystems, Foster City, USA).

    Electrophoresis:

    Article Title: Molecular evidence for hybridization in Colias (Lepidoptera: Pieridae): are Colias hybrids really hybrids?
    Article Snippet: .. AFLP amplicon data were collected on an ABI 3130XL Capillary Electrophoresis Genetic Analyzer (Applied Biosystems, Foster City, CA, United States) and compiled for each primer combination with ABI GeneMapper software version 3.7 (Life Technologies, Carlsbad, CA, United States). ..

    Polymerase Chain Reaction:

    Article Title: Foot-and-Mouth Disease Virus-Associated Abortion and Vertical Transmission following Acute Infection in Cattle under Natural Conditions
    Article Snippet: .. For phylogenetic comparisons, PCR amplification of VP1 coding regions of FMDV were performed using the primer combination of ARS4 [ ] and NK61 in combination with Fermentas Pfu DNA polymerase (Thermo Fisher Scientific, Waltham, MA USA) as previously described [ ]. .. The PCR products were purified using QIA quick Gel Extraction Kit (QIAGEN, Hilden, Germany).

    Article Title: Molecular Approach to the Identification of Fish in the South China Sea
    Article Snippet: .. PCR amplifications were performed with the following conditions: 35 cycles of denaturation at 94°C for 45 s, annealing at 52–62°C (depending on the primer combination) for 50 s, and extension at 72°C for 1 min, with an initial denaturation at 94°C for 2 min and final extension at 72°C for 5 min. Amplified products were visualized in 1% agarose gel, and purified products were directly sequenced on an Applied Biosystems 3730 sequencer using the BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Biosystems, Inc.). ..

    Article Title: Linkage mapping reveals sex-dimorphic map distances in a passerine bird
    Article Snippet: .. One primer in each primer combination was labelled with fluorescent dye and the PCR-products were separated and the alleles detected in an ABI 3730 capillary sequencer (Applied Biosystems). .. When possible, several loci per individual were run simultaneously in the sequencer, and each run included a size ladder (ABI GeneScan LIZ500; Applied Biosystems).

    Article Title: Host-associated differentiation in a highly polyphagous, sexually reproducing insect herbivore
    Article Snippet: .. Selective PCR amplifications were performed in a 21-μ L volume of 15 μ L platinum supermix (Life Technologies), 4 μ L of a 19-fold diluted pre-amplification reaction product, and one primer combination consisting of 1 μ L MseI-CAT (Life Technologies) and 1 μ L EcoRI-ACT (Life Technologies). .. All PCR amplifications were carried out in an ABI GeneAmp thermocycler (Life Technologies) using protocols from Barman et al. ( ).

    Article Title: Body Size Adaptations to Altitudinal Climatic Variation in Neotropical Grasshoppers of the Genus Sphenarium (Orthoptera: Pyrgomorphidae)
    Article Snippet: .. Long-PCR conditions included 2 min of initial denaturation at 92°C; 39 cycles of 92°C for 30 s, 50°C for 30 s, 60°C for 5 min; and a final extension at 60°C for 20 min. Other PCR reactions were performed using an initial denaturation at 92°C for 2 min, followed by 34 cycles of 92°C for 30 s, 30 s at the specific Tm for each primer combination (see ), 2 min at 60°C; and a final extension at 60°C for 10 min. Single-band PCR products were purified using PrepEase Purification 96-well plate kit (USB Corporation, Santa Clara, USA) and samples containing more than a single band were purified using QIAquick Gel Extraction Kit (Qiagen, Valencia, USA). .. We sequenced both strands of each purified products using BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) using Applied Biosystems 3730XL DNA Analyzer (Applied Biosystems, Foster City, USA).

    Sequencing:

    Article Title: Molecular Approach to the Identification of Fish in the South China Sea
    Article Snippet: .. PCR amplifications were performed with the following conditions: 35 cycles of denaturation at 94°C for 45 s, annealing at 52–62°C (depending on the primer combination) for 50 s, and extension at 72°C for 1 min, with an initial denaturation at 94°C for 2 min and final extension at 72°C for 5 min. Amplified products were visualized in 1% agarose gel, and purified products were directly sequenced on an Applied Biosystems 3730 sequencer using the BigDye Terminator Cycle Sequencing Ready Reaction Kit (PE Biosystems, Inc.). ..

    Article Title: MHC class II DRB diversity, selection pattern and population structure in a neotropical bat species, Noctilio albiventris
    Article Snippet: .. At least 23 recombinant clones per primer combination were amplified using the vector primers T7for and M13rev, sequenced using BigDye Terminator v.3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and analysed on ABI PRISM 310 Automated Genetic Analyzer (Applied Biosystems). .. All primer combinations amplified the same two MHC class II DRB alleles in N. albiventris .

    Gel Extraction:

    Article Title: Body Size Adaptations to Altitudinal Climatic Variation in Neotropical Grasshoppers of the Genus Sphenarium (Orthoptera: Pyrgomorphidae)
    Article Snippet: .. Long-PCR conditions included 2 min of initial denaturation at 92°C; 39 cycles of 92°C for 30 s, 50°C for 30 s, 60°C for 5 min; and a final extension at 60°C for 20 min. Other PCR reactions were performed using an initial denaturation at 92°C for 2 min, followed by 34 cycles of 92°C for 30 s, 30 s at the specific Tm for each primer combination (see ), 2 min at 60°C; and a final extension at 60°C for 10 min. Single-band PCR products were purified using PrepEase Purification 96-well plate kit (USB Corporation, Santa Clara, USA) and samples containing more than a single band were purified using QIAquick Gel Extraction Kit (Qiagen, Valencia, USA). .. We sequenced both strands of each purified products using BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) using Applied Biosystems 3730XL DNA Analyzer (Applied Biosystems, Foster City, USA).

    Recombinant:

    Article Title: MHC class II DRB diversity, selection pattern and population structure in a neotropical bat species, Noctilio albiventris
    Article Snippet: .. At least 23 recombinant clones per primer combination were amplified using the vector primers T7for and M13rev, sequenced using BigDye Terminator v.3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and analysed on ABI PRISM 310 Automated Genetic Analyzer (Applied Biosystems). .. All primer combinations amplified the same two MHC class II DRB alleles in N. albiventris .

    Plasmid Preparation:

    Article Title: MHC class II DRB diversity, selection pattern and population structure in a neotropical bat species, Noctilio albiventris
    Article Snippet: .. At least 23 recombinant clones per primer combination were amplified using the vector primers T7for and M13rev, sequenced using BigDye Terminator v.3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and analysed on ABI PRISM 310 Automated Genetic Analyzer (Applied Biosystems). .. All primer combinations amplified the same two MHC class II DRB alleles in N. albiventris .

    Software:

    Article Title: Molecular evidence for hybridization in Colias (Lepidoptera: Pieridae): are Colias hybrids really hybrids?
    Article Snippet: .. AFLP amplicon data were collected on an ABI 3130XL Capillary Electrophoresis Genetic Analyzer (Applied Biosystems, Foster City, CA, United States) and compiled for each primer combination with ABI GeneMapper software version 3.7 (Life Technologies, Carlsbad, CA, United States). ..

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
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  • 91
    Thermo Fisher primer combinations
    Primer Combinations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer combinations/product/Thermo Fisher
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    primer combinations - by Bioz Stars, 2020-07
    91/100 stars
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