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TaKaRa primer combination
Primer Combination, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primer combination/product/TaKaRa
Average 90 stars, based on 5 article reviews
Price from $9.99 to $1999.99
primer combination - by Bioz Stars, 2020-07
90/100 stars

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Polymerase Chain Reaction:

Article Title: Characterization of the Complete Mitochondrion Genome of Diurnal Moth Amata emma (Butler) (Lepidoptera: Erebidae) and Its Phylogenetic Implications
Article Snippet: .. PCR amplification conditions were as follows: an initial denaturation for 5 min at 95°C, followed by 35 cycles of denaturation for 30 s at 94°C, annealing for 30 s at 48–55°C (depending on primer combination), elongation for 1–3 min (depending on putative length of the fragments) at 68°C, and a final extension step of 72°C for 10 min. All amplifications applied Takara LA Taq (Takara Co., Dalian, China) and performed on an Eppendorf Mastercycler and Mastercycler gradient. .. The PCR products were resolved by electrophoresis in 1.0% agarose gel, purified using 3Spin PCR Product Purification Kit.

Article Title: Gene expression changes during caste-specific neuronal development in the damp-wood termite Hodotermopsis sjostedti
Article Snippet: .. Subcloning and sequencing Bands of interest were excised, gel slices were boiled in 100 μl of distilled water, and the DNA was re-amplified by PCR with the primer combination used in FDD with an Advantage cDNA PCR Kit (Clontech Laboratories, Inc.). ..

Article Title: Biochemical Characterization of CYP505D6, a Self-Sufficient Cytochrome P450 from the White-Rot Fungus Phanerochaete chrysosporium
Article Snippet: .. A full-length P. chrysosporium CYP505D6 gene was PCR amplified by using the primer combination specified in , using a 2400 DNA thermal cycler (TaKaRa Bio, Otsu, Japan), as follows: an initial denaturation step at 95°C for 3 min, followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 62°C for 30 s, and extension at 72°C for 3 min. ..

Sequencing:

Article Title: Gene expression changes during caste-specific neuronal development in the damp-wood termite Hodotermopsis sjostedti
Article Snippet: .. Subcloning and sequencing Bands of interest were excised, gel slices were boiled in 100 μl of distilled water, and the DNA was re-amplified by PCR with the primer combination used in FDD with an Advantage cDNA PCR Kit (Clontech Laboratories, Inc.). ..

Article Title: Dissecting Defense-Related and Developmental Transcriptional Responses of Maize during Ustilago maydis Infection and Subsequent Tumor Formation 1
Article Snippet: .. To confirm the deduced ORF sequence of Umi2 the entire ORF was amplified from the same cDNA preparation using the primer combination (5′-ACCATGGCTGCCCCAACACTAACTGC-3′/5′-CATGAGTACCGGCTTCACATAAAGC-3′) and Platinum Pfu polymerase (CLONTECH, Heidelberg). .. For RNA-blot analysis cloned fragments from differential display analysis were either isolated from pUC19 using Eco RI/ Pst I ( Umi4 , 5 , 6 , 12 , Umr1 ) or from pCR2.1-TOPO using Hin dIII/ Xba I ( Umi7 , 8 , 9 , 11 , Umr2 ).

Subcloning:

Article Title: Gene expression changes during caste-specific neuronal development in the damp-wood termite Hodotermopsis sjostedti
Article Snippet: .. Subcloning and sequencing Bands of interest were excised, gel slices were boiled in 100 μl of distilled water, and the DNA was re-amplified by PCR with the primer combination used in FDD with an Advantage cDNA PCR Kit (Clontech Laboratories, Inc.). ..

Amplification:

Article Title: Characterization of the Complete Mitochondrion Genome of Diurnal Moth Amata emma (Butler) (Lepidoptera: Erebidae) and Its Phylogenetic Implications
Article Snippet: .. PCR amplification conditions were as follows: an initial denaturation for 5 min at 95°C, followed by 35 cycles of denaturation for 30 s at 94°C, annealing for 30 s at 48–55°C (depending on primer combination), elongation for 1–3 min (depending on putative length of the fragments) at 68°C, and a final extension step of 72°C for 10 min. All amplifications applied Takara LA Taq (Takara Co., Dalian, China) and performed on an Eppendorf Mastercycler and Mastercycler gradient. .. The PCR products were resolved by electrophoresis in 1.0% agarose gel, purified using 3Spin PCR Product Purification Kit.

Article Title: Dissecting Defense-Related and Developmental Transcriptional Responses of Maize during Ustilago maydis Infection and Subsequent Tumor Formation 1
Article Snippet: .. To confirm the deduced ORF sequence of Umi2 the entire ORF was amplified from the same cDNA preparation using the primer combination (5′-ACCATGGCTGCCCCAACACTAACTGC-3′/5′-CATGAGTACCGGCTTCACATAAAGC-3′) and Platinum Pfu polymerase (CLONTECH, Heidelberg). .. For RNA-blot analysis cloned fragments from differential display analysis were either isolated from pUC19 using Eco RI/ Pst I ( Umi4 , 5 , 6 , 12 , Umr1 ) or from pCR2.1-TOPO using Hin dIII/ Xba I ( Umi7 , 8 , 9 , 11 , Umr2 ).

Article Title: Biochemical Characterization of CYP505D6, a Self-Sufficient Cytochrome P450 from the White-Rot Fungus Phanerochaete chrysosporium
Article Snippet: .. A full-length P. chrysosporium CYP505D6 gene was PCR amplified by using the primer combination specified in , using a 2400 DNA thermal cycler (TaKaRa Bio, Otsu, Japan), as follows: an initial denaturation step at 95°C for 3 min, followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 62°C for 30 s, and extension at 72°C for 3 min. ..

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    TaKaRa primer combinations
    Primer Combinations, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer combinations/product/TaKaRa
    Average 91 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    primer combinations - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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